Click here to switch to the map view.

The map label for this gene is gcvH

Identifier: 52787153

GI number: 52787153

Start: 3304300

End: 3304683

Strand: Reverse

Name: gcvH

Synonym: BLi03462

Alternate gene names: 52787153

Gene position: 3304683-3304300 (Counterclockwise)

Preceding gene: 52787154

Following gene: 52787152

Centisome position: 78.26

GC content: 46.88

Gene sequence:

>384_bases
ATGAGCACACCGAAAGAGTTGCGTTATTCAGAAGAACACGAATGGGTGAAAACCGAAGGAGATAAAGTCAGAATCGGCAT
TACAGACTTTGCACAATCTGAGCTTGGAGACATCGTATTTGTCGAGCTTCCTGAAGTGGGAGACGAAATCAAAGCGGACG
AGCCTTTTGGCAGCGTTGAATCCGTAAAGACCGTTTCTGAGCTGTATGCCCCGATCAACGGAAAAGTGGTCGAAGTGAAC
GAAGATCTGGAAGACAGCCCTGAATTCGTCAACGAGTCTCCGTATGAAAAGGCTTGGATGATTGTCGTCGAGCCGTCAGA
CGCTTCTGAAATCGAAAACCTGATGACAGCCGGGCAATATGAGGACATGATAAAAGAAGACTAA

Upstream 100 bases:

>100_bases
GGTTTTAAAGAAGATCAGTTTAAGACATACTGGTTATAACACATCAAAATATGATATCTTCAATGTAGGATCGATTAAAA
CACTTTATGGAGGGGTTAGC

Downstream 100 bases:

>100_bases
ACAGGATAGACATTCGTCGTTTGGATACTCTATATCAGGAGGGGGTGTCCAAATTGACGCTGAAAAAAGAACGTATTGAT
ATTACAGAACATGTGGAAAG

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 127; Mature: 126

Protein sequence:

>127_residues
MSTPKELRYSEEHEWVKTEGDKVRIGITDFAQSELGDIVFVELPEVGDEIKADEPFGSVESVKTVSELYAPINGKVVEVN
EDLEDSPEFVNESPYEKAWMIVVEPSDASEIENLMTAGQYEDMIKED

Sequences:

>Translated_127_residues
MSTPKELRYSEEHEWVKTEGDKVRIGITDFAQSELGDIVFVELPEVGDEIKADEPFGSVESVKTVSELYAPINGKVVEVN
EDLEDSPEFVNESPYEKAWMIVVEPSDASEIENLMTAGQYEDMIKED
>Mature_126_residues
STPKELRYSEEHEWVKTEGDKVRIGITDFAQSELGDIVFVELPEVGDEIKADEPFGSVESVKTVSELYAPINGKVVEVNE
DLEDSPEFVNESPYEKAWMIVVEPSDASEIENLMTAGQYEDMIKED

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=118, Percent_Identity=46.6101694915254, Blast_Score=120, Evalue=3e-28,
Organism=Homo sapiens, GI89057342, Length=118, Percent_Identity=45.7627118644068, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1789271, Length=127, Percent_Identity=46.4566929133858, Blast_Score=125, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI17551294, Length=119, Percent_Identity=43.6974789915966, Blast_Score=112, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17507493, Length=119, Percent_Identity=43.6974789915966, Blast_Score=110, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6319272, Length=128, Percent_Identity=42.96875, Blast_Score=111, Evalue=3e-26,
Organism=Drosophila melanogaster, GI17865652, Length=121, Percent_Identity=47.1074380165289, Blast_Score=123, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_BACLD (Q65F75)

Other databases:

- EMBL:   CP000002
- EMBL:   AE017333
- RefSeq:   YP_080558.1
- RefSeq:   YP_092982.1
- HSSP:   P16048
- ProteinModelPortal:   Q65F75
- SMR:   Q65F75
- STRING:   Q65F75
- EnsemblBacteria:   EBBACT00000056050
- EnsemblBacteria:   EBBACT00000060801
- GeneID:   3027665
- GeneID:   3099944
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi03462
- KEGG:   bli:BL02176
- NMPDR:   fig|279010.5.peg.3707
- eggNOG:   COG0509
- GeneTree:   EBGT00050000002634
- HOGENOM:   HBG693789
- OMA:   TPKELRY
- ProtClustDB:   PRK01202
- BioCyc:   BLIC279010-1:BLI03462-MONOMER
- BioCyc:   BLIC279010:BL02176-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 14319; Mature: 14188

Theoretical pI: Translated: 3.76; Mature: 3.76

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPKELRYSEEHEWVKTEGDKVRIGITDFAQSELGDIVFVELPEVGDEIKADEPFGSVE
CCCCHHCCCCCCCCEEECCCCEEEEEEHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCHH
SVKTVSELYAPINGKVVEVNEDLEDSPEFVNESPYEKAWMIVVEPSDASEIENLMTAGQY
HHHHHHHHHCCCCCEEEEECCCCCCCCHHCCCCCCCCEEEEEECCCCHHHHHHHHHCCHH
EDMIKED
HHHHCCC
>Mature Secondary Structure 
STPKELRYSEEHEWVKTEGDKVRIGITDFAQSELGDIVFVELPEVGDEIKADEPFGSVE
CCCHHCCCCCCCCEEECCCCEEEEEEHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCHH
SVKTVSELYAPINGKVVEVNEDLEDSPEFVNESPYEKAWMIVVEPSDASEIENLMTAGQY
HHHHHHHHHCCCCCEEEEECCCCCCCCHHCCCCCCCCEEEEEECCCCHHHHHHHHHCCHH
EDMIKED
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA