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The map label for this gene is lipA [H]

Identifier: 52787114

GI number: 52787114

Start: 3266795

End: 3267706

Strand: Reverse

Name: lipA [H]

Synonym: BLi03422

Alternate gene names: 52787114

Gene position: 3267706-3266795 (Counterclockwise)

Preceding gene: 52787116

Following gene: 52787112

Centisome position: 77.39

GC content: 49.45

Gene sequence:

>912_bases
ATGGAGATGATGGAATTGGCAAAGAAAGACGAGCACCTAAGAAAGCCCGAATGGCTTAAAATCAAGCTGAACACGAATGA
AAACTATACGGGCTTAAAAAAATTAATGCGGGAAAACAACCTCCATACGGTCTGCGAAGAGGCAAAATGCCCGAACATCC
ACGAATGCTGGGCCGTCAGACGTACCGCGACCTTTATGATTCTCGGATCTGTCTGTACGAGAGCCTGCCGCTTCTGTGCG
GTGAAGACGGGGCTGCCGACAGAGCTTGACTTGAATGAGCCGGAAAGAGTCGCAGATTCTGTGAGCATCATGAACCTGAA
ACACGCGGTCATTACAGCCGTTGCCCGTGACGATTTAAAAGACGGAGGAGCGGGGGTATTCGCCGAAACCGTACGCGCCG
TCCGGAGAAAAAGCCCATTTACAACAATCGAAGTTCTGCCGTCCGATATGGGCGGAGACTATAATAATTTGAAAACGCTG
ATGGATACGCGTCCGGACATTTTAAACCATAATATTGAAACGGTCCGCCGCCTGACGCCGAGAGTCCGTGCGAGAGCTAC
ATATGAAAGATCATTGGAATTTTTGCGCCGCGCCAAAGAGATGCAGCCGGACATCCCGACGAAATCCAGCATCATGATCG
GTCTTGGCGAAACAAAAGAAGAAATCATCGAAACAATGGACGACCTTCTTGCGAACAACGTCGACATCATGGCAATCGGC
CAGTATCTGCAGCCGTCCAAAAAACACATTAAAGTACAGAAATATTACCATCCTGACGAGTTTGCCGAACTGAAAGAAAT
CGCCATGGAAAAGGGCTTCAGCCACTGTGAAGCAGGACCGCTCGTCCGTTCTTCATACCATGCCGACGAACAGGTGAATG
AAGCGTCTAAAAAACGCCAGGCGCAGGCATAA

Upstream 100 bases:

>100_bases
AGATGAAGATTTAGCATTTTTTATTTAAGCGGTTCCAAAGCAGAGGGACTCATCCGGCGCTATGTCACAAATTTCGTATT
GAACATGACTACTATCCACA

Downstream 100 bases:

>100_bases
CATTGGATGAAAAAACGCCAGCCATATGCGCTGGCGTTTTTCTTATTTTCAGGTCAAGATTCGGTCGCCTTTCCTTTTCC
GTCAAGGTCGTTTCCGTTTT

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MEMMELAKKDEHLRKPEWLKIKLNTNENYTGLKKLMRENNLHTVCEEAKCPNIHECWAVRRTATFMILGSVCTRACRFCA
VKTGLPTELDLNEPERVADSVSIMNLKHAVITAVARDDLKDGGAGVFAETVRAVRRKSPFTTIEVLPSDMGGDYNNLKTL
MDTRPDILNHNIETVRRLTPRVRARATYERSLEFLRRAKEMQPDIPTKSSIMIGLGETKEEIIETMDDLLANNVDIMAIG
QYLQPSKKHIKVQKYYHPDEFAELKEIAMEKGFSHCEAGPLVRSSYHADEQVNEASKKRQAQA

Sequences:

>Translated_303_residues
MEMMELAKKDEHLRKPEWLKIKLNTNENYTGLKKLMRENNLHTVCEEAKCPNIHECWAVRRTATFMILGSVCTRACRFCA
VKTGLPTELDLNEPERVADSVSIMNLKHAVITAVARDDLKDGGAGVFAETVRAVRRKSPFTTIEVLPSDMGGDYNNLKTL
MDTRPDILNHNIETVRRLTPRVRARATYERSLEFLRRAKEMQPDIPTKSSIMIGLGETKEEIIETMDDLLANNVDIMAIG
QYLQPSKKHIKVQKYYHPDEFAELKEIAMEKGFSHCEAGPLVRSSYHADEQVNEASKKRQAQA
>Mature_303_residues
MEMMELAKKDEHLRKPEWLKIKLNTNENYTGLKKLMRENNLHTVCEEAKCPNIHECWAVRRTATFMILGSVCTRACRFCA
VKTGLPTELDLNEPERVADSVSIMNLKHAVITAVARDDLKDGGAGVFAETVRAVRRKSPFTTIEVLPSDMGGDYNNLKTL
MDTRPDILNHNIETVRRLTPRVRARATYERSLEFLRRAKEMQPDIPTKSSIMIGLGETKEEIIETMDDLLANNVDIMAIG
QYLQPSKKHIKVQKYYHPDEFAELKEIAMEKGFSHCEAGPLVRSSYHADEQVNEASKKRQAQA

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=289, Percent_Identity=43.9446366782007, Blast_Score=259, Evalue=2e-69,
Organism=Homo sapiens, GI37577164, Length=251, Percent_Identity=44.2231075697211, Blast_Score=224, Evalue=6e-59,
Organism=Escherichia coli, GI1786846, Length=281, Percent_Identity=53.0249110320285, Blast_Score=299, Evalue=1e-82,
Organism=Caenorhabditis elegans, GI32564533, Length=296, Percent_Identity=45.2702702702703, Blast_Score=249, Evalue=2e-66,
Organism=Saccharomyces cerevisiae, GI6324770, Length=297, Percent_Identity=46.8013468013468, Blast_Score=271, Evalue=9e-74,
Organism=Drosophila melanogaster, GI221513272, Length=302, Percent_Identity=46.3576158940397, Blast_Score=265, Evalue=4e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 34517; Mature: 34517

Theoretical pI: Translated: 7.82; Mature: 7.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMMELAKKDEHLRKPEWLKIKLNTNENYTGLKKLMRENNLHTVCEEAKCPNIHECWAVR
CCHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHH
RTATFMILGSVCTRACRFCAVKTGLPTELDLNEPERVADSVSIMNLKHAVITAVARDDLK
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
DGGAGVFAETVRAVRRKSPFTTIEVLPSDMGGDYNNLKTLMDTRPDILNHNIETVRRLTP
CCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHCCHHHHCCCHHHHHHHHH
RVRARATYERSLEFLRRAKEMQPDIPTKSSIMIGLGETKEEIIETMDDLLANNVDIMAIG
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCEEEC
QYLQPSKKHIKVQKYYHPDEFAELKEIAMEKGFSHCEAGPLVRSSYHADEQVNEASKKRQ
HHHCCCHHCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCHHHHHHHHHHHH
AQA
CCC
>Mature Secondary Structure
MEMMELAKKDEHLRKPEWLKIKLNTNENYTGLKKLMRENNLHTVCEEAKCPNIHECWAVR
CCHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHH
RTATFMILGSVCTRACRFCAVKTGLPTELDLNEPERVADSVSIMNLKHAVITAVARDDLK
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
DGGAGVFAETVRAVRRKSPFTTIEVLPSDMGGDYNNLKTLMDTRPDILNHNIETVRRLTP
CCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHCCHHHHCCCHHHHHHHHH
RVRARATYERSLEFLRRAKEMQPDIPTKSSIMIGLGETKEEIIETMDDLLANNVDIMAIG
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCEEEC
QYLQPSKKHIKVQKYYHPDEFAELKEIAMEKGFSHCEAGPLVRSSYHADEQVNEASKKRQ
HHHCCCHHCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCHHHHHHHHHHHH
AQA
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA