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The map label for this gene is dapF

Identifier: 52787092

GI number: 52787092

Start: 3247958

End: 3248815

Strand: Reverse

Name: dapF

Synonym: BLi03399

Alternate gene names: 52787092

Gene position: 3248815-3247958 (Counterclockwise)

Preceding gene: 52787093

Following gene: 52787091

Centisome position: 76.94

GC content: 47.32

Gene sequence:

>858_bases
ATGAATTCTTTTCGTTTTACTAAAATGCACGGCTTAGGAAACAGCTATATATACGTCAATCAGTTTGAAGAACATCTCCC
TGAGGAGCTGTTGTCGGATTTGGCCGTCAAAGTGTCTTCCGTTTACACCGGAATCGGTTCAGACGGAATGATTCTCATCT
GTCCTTCAGATCGAGCGCCGGTTAAAATGCGGATTTTCAACAGCGACGGATCTGAAGGCAAAAACTGCGGAAACGGTTTG
CGCTGCGTTGCAAAATACGCGTATGAACATAAACTGGTAGAGGAAACGTCCTTTTTAATTGAAACCCTTTCAGGCTTGGT
AAAGGCTCAGGTTGAGGTGGATGAAGGAAAAGTTGTATCGGCTACGGTCGATATGGGCGAACCGCGACTTCTCAAATCAG
ATATGCCGATGCGGGGTGAACAGGGTTCAGAGACGATCAATGAAAAGATGATATTCGGCGGCCGTGAAATGAAGGGTACC
GCTGTATCCATGGGCAATCCCCACATCGTCTTTTATTTAGACGATATTGAAAAAGCCCCGCTGACGACGATGGGACCGCT
CATTGAAAAAGATGAAAGGTTTCCGGAAGGGGTTAATGTAGAATTTGTTGAAGTTGAAAACGAAAATGAACTCCATTTCC
GCGTATGGGAAAGGGGATCAGGAATTACCCAGGCTTGCGGAACAGGCGCCTGCGCAGCCGCAGTGAGTTCGGTGCTCAAC
GGCTATTCCAAACGGGACACGGACATTACCGTCCATCTCGCCGGCGGCGATTTGATCATTAATTGGAAAAACGACGGACG
CGTCATGATGACGGGACCCGCTGAAACGGTATGTGAAGGCGAGTTTTTTATATCATAA

Upstream 100 bases:

>100_bases
ATGCAGGGAGCTTTTATGTATGAAAAACGGGGGATAGCCATATTGTATCCATCCCTTTCAGCTTTTATACTAGAAGAAAG
AAAAAAATGTGGAGGCGGCT

Downstream 100 bases:

>100_bases
GTCGGTCTGTTTGAGAAATCGGACGTGCGACTTTATAATAAAGGTACACATGAGCTTTTAGGAGGTGTAATCTCATGAGC
GAAAAAACGGTCACGGTTAC

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MNSFRFTKMHGLGNSYIYVNQFEEHLPEELLSDLAVKVSSVYTGIGSDGMILICPSDRAPVKMRIFNSDGSEGKNCGNGL
RCVAKYAYEHKLVEETSFLIETLSGLVKAQVEVDEGKVVSATVDMGEPRLLKSDMPMRGEQGSETINEKMIFGGREMKGT
AVSMGNPHIVFYLDDIEKAPLTTMGPLIEKDERFPEGVNVEFVEVENENELHFRVWERGSGITQACGTGACAAAVSSVLN
GYSKRDTDITVHLAGGDLIINWKNDGRVMMTGPAETVCEGEFFIS

Sequences:

>Translated_285_residues
MNSFRFTKMHGLGNSYIYVNQFEEHLPEELLSDLAVKVSSVYTGIGSDGMILICPSDRAPVKMRIFNSDGSEGKNCGNGL
RCVAKYAYEHKLVEETSFLIETLSGLVKAQVEVDEGKVVSATVDMGEPRLLKSDMPMRGEQGSETINEKMIFGGREMKGT
AVSMGNPHIVFYLDDIEKAPLTTMGPLIEKDERFPEGVNVEFVEVENENELHFRVWERGSGITQACGTGACAAAVSSVLN
GYSKRDTDITVHLAGGDLIINWKNDGRVMMTGPAETVCEGEFFIS
>Mature_285_residues
MNSFRFTKMHGLGNSYIYVNQFEEHLPEELLSDLAVKVSSVYTGIGSDGMILICPSDRAPVKMRIFNSDGSEGKNCGNGL
RCVAKYAYEHKLVEETSFLIETLSGLVKAQVEVDEGKVVSATVDMGEPRLLKSDMPMRGEQGSETINEKMIFGGREMKGT
AVSMGNPHIVFYLDDIEKAPLTTMGPLIEKDERFPEGVNVEFVEVENENELHFRVWERGSGITQACGTGACAAAVSSVLN
GYSKRDTDITVHLAGGDLIINWKNDGRVMMTGPAETVCEGEFFIS

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=285, Percent_Identity=36.8421052631579, Blast_Score=173, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_BACLD (Q65FD6)

Other databases:

- EMBL:   CP000002
- EMBL:   AE017333
- RefSeq:   YP_080498.1
- RefSeq:   YP_092921.1
- HSSP:   P44859
- ProteinModelPortal:   Q65FD6
- SMR:   Q65FD6
- STRING:   Q65FD6
- EnsemblBacteria:   EBBACT00000057179
- EnsemblBacteria:   EBBACT00000059593
- GeneID:   3027547
- GeneID:   3099737
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi03399
- KEGG:   bli:BL03150
- NMPDR:   fig|279010.5.peg.3646
- eggNOG:   COG0253
- GeneTree:   EBGT00050000002647
- HOGENOM:   HBG399442
- OMA:   HTGIGFD
- ProtClustDB:   PRK00450
- BioCyc:   BLIC279010-1:BLI03399-MONOMER
- BioCyc:   BLIC279010:BL03150-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 31268; Mature: 31268

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 76-76 ACT_SITE 226-226

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSFRFTKMHGLGNSYIYVNQFEEHLPEELLSDLAVKVSSVYTGIGSDGMILICPSDRAP
CCCCEEEEECCCCCCEEEEECHHHHCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCC
VKMRIFNSDGSEGKNCGNGLRCVAKYAYEHKLVEETSFLIETLSGLVKAQVEVDEGKVVS
EEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEEE
ATVDMGEPRLLKSDMPMRGEQGSETINEKMIFGGREMKGTAVSMGNPHIVFYLDDIEKAP
EEECCCCCEEECCCCCCCCCCCCHHHHHHEEECCCCCCCEEEECCCCEEEEEECCCCCCC
LTTMGPLIEKDERFPEGVNVEFVEVENENELHFRVWERGSGITQACGTGACAAAVSSVLN
CCHHCCHHHCCCCCCCCCCEEEEEECCCCCEEEEEEECCCCCHHHCCCCHHHHHHHHHHC
GYSKRDTDITVHLAGGDLIINWKNDGRVMMTGPAETVCEGEFFIS
CCCCCCCEEEEEEECCEEEEEECCCCEEEEECCCCCEECCEEEEC
>Mature Secondary Structure
MNSFRFTKMHGLGNSYIYVNQFEEHLPEELLSDLAVKVSSVYTGIGSDGMILICPSDRAP
CCCCEEEEECCCCCCEEEEECHHHHCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCC
VKMRIFNSDGSEGKNCGNGLRCVAKYAYEHKLVEETSFLIETLSGLVKAQVEVDEGKVVS
EEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEEE
ATVDMGEPRLLKSDMPMRGEQGSETINEKMIFGGREMKGTAVSMGNPHIVFYLDDIEKAP
EEECCCCCEEECCCCCCCCCCCCHHHHHHEEECCCCCCCEEEECCCCEEEEEECCCCCCC
LTTMGPLIEKDERFPEGVNVEFVEVENENELHFRVWERGSGITQACGTGACAAAVSSVLN
CCHHCCHHHCCCCCCCCCCEEEEEECCCCCEEEEEEECCCCCHHHCCCCHHHHHHHHHHC
GYSKRDTDITVHLAGGDLIINWKNDGRVMMTGPAETVCEGEFFIS
CCCCCCCEEEEEEECCEEEEEECCCCEEEEECCCCCEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA