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The map label for this gene is yueD [H]

Identifier: 52787068

GI number: 52787068

Start: 3221458

End: 3222189

Strand: Reverse

Name: yueD [H]

Synonym: BLi03373

Alternate gene names: 52787068

Gene position: 3222189-3221458 (Counterclockwise)

Preceding gene: 52787069

Following gene: 52787067

Centisome position: 76.31

GC content: 46.86

Gene sequence:

>732_bases
ATGCATTTATCCATTGTTACCGGAGCGTCAAAAGGGCTTGGCGCCGCGATGGCCGAAGGGCTTTTGCAAAAAGGCCATAT
TGTTCATGCTGTTTCAAGGACCAAGTCTGATATCAGTCATGACAGGCTGATCCAGCATGAGGCCGATCTGGCGCAGCTCG
CCGAAACAGAAAAGTGGTTTCGGCAATTCTCAGATTCTGTCCAAGCCCATGATTTTGCTTCGATCACCTTGATCAATAAT
GCGGGAATGGTGACGCCGATCAAGCGCGCCGGCGAAGCTGAACCGGAAGAACTGAGGCTGCATTATGACTTGAATTTGTT
AGCGCCGGTGATGCTCAGCCAGCTGTTTACCAAATGGCTCAAGCCATATAAAGGCAAAAAATCGATTGTCAATATATCAT
CAGGCGCGGCCAAAAACCCTTATAAAGGATGGAGTGCCTACTGCAGCTCAAAAGCGGGGCTCGATATGTTCACGAAAACA
TTCGGCTTTGAACAGGAAGATGAGTCTGATCCGGTGAAGATGATTTCTTTCTCCCCCGGGATCATGGATACCGGCATGCA
AGAGGTTATCCGTTCATCATCGAAAAAAGATTTTCATGAAATTGAACGATTCAAAACCTATCAAACAGAAGGAAAGCTGA
GAAGCACCTCTTATGTGGCCGGCATTCTGCTCGACCTGCTTGAGACCGACTTTGAAAACGGCAGAGTGTATGATATTAAA
GAGTTTTTGTAA

Upstream 100 bases:

>100_bases
TGCGGCTGTCTTTTGGTTGGCTGTTCTTAGCGCAGAAAACCGCAGCACTTGAGAAGCGGGCTGATGAATCAGTACAATAA
TGGATAAGGAGGGACGCCGT

Downstream 100 bases:

>100_bases
AAAATAAAAAAACAAGCACATCGAATCGATTGTGCTTGTTTTTTATTTATCAATTTTGAATATGATGTGCCAGATCAATT
GAGATTTGCATGAAAATATG

Product: short chain dehydrogenase

Products: 2,3-Dihydroxybenzoate; H+; Nicotinamide adenine dinucleotide - reduced [C]

Alternate protein names: NA

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MHLSIVTGASKGLGAAMAEGLLQKGHIVHAVSRTKSDISHDRLIQHEADLAQLAETEKWFRQFSDSVQAHDFASITLINN
AGMVTPIKRAGEAEPEELRLHYDLNLLAPVMLSQLFTKWLKPYKGKKSIVNISSGAAKNPYKGWSAYCSSKAGLDMFTKT
FGFEQEDESDPVKMISFSPGIMDTGMQEVIRSSSKKDFHEIERFKTYQTEGKLRSTSYVAGILLDLLETDFENGRVYDIK
EFL

Sequences:

>Translated_243_residues
MHLSIVTGASKGLGAAMAEGLLQKGHIVHAVSRTKSDISHDRLIQHEADLAQLAETEKWFRQFSDSVQAHDFASITLINN
AGMVTPIKRAGEAEPEELRLHYDLNLLAPVMLSQLFTKWLKPYKGKKSIVNISSGAAKNPYKGWSAYCSSKAGLDMFTKT
FGFEQEDESDPVKMISFSPGIMDTGMQEVIRSSSKKDFHEIERFKTYQTEGKLRSTSYVAGILLDLLETDFENGRVYDIK
EFL
>Mature_243_residues
MHLSIVTGASKGLGAAMAEGLLQKGHIVHAVSRTKSDISHDRLIQHEADLAQLAETEKWFRQFSDSVQAHDFASITLINN
AGMVTPIKRAGEAEPEELRLHYDLNLLAPVMLSQLFTKWLKPYKGKKSIVNISSGAAKNPYKGWSAYCSSKAGLDMFTKT
FGFEQEDESDPVKMISFSPGIMDTGMQEVIRSSSKKDFHEIERFKTYQTEGKLRSTSYVAGILLDLLETDFENGRVYDIK
EFL

Specific function: Reduces benzil stereospecifically to (S)-benzoin [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI4507185, Length=202, Percent_Identity=28.2178217821782, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI7705925, Length=227, Percent_Identity=28.6343612334802, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI115534694, Length=191, Percent_Identity=28.2722513089005, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6322227, Length=241, Percent_Identity=28.2157676348548, Blast_Score=90, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6322226, Length=213, Percent_Identity=28.6384976525822, Blast_Score=82, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24640597, Length=199, Percent_Identity=27.1356783919598, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI23397609, Length=196, Percent_Identity=30.6122448979592, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.3.1.28 [C]

Molecular weight: Translated: 27185; Mature: 27185

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLSIVTGASKGLGAAMAEGLLQKGHIVHAVSRTKSDISHDRLIQHEADLAQLAETEKWF
CEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RQFSDSVQAHDFASITLINNAGMVTPIKRAGEAEPEELRLHYDLNLLAPVMLSQLFTKWL
HHHHHHHHHCCCEEEEEECCCCCCCCHHHCCCCCHHHEEEEECHHHHHHHHHHHHHHHHH
KPYKGKKSIVNISSGAAKNPYKGWSAYCSSKAGLDMFTKTFGFEQEDESDPVKMISFSPG
CCCCCCCCEEEECCCCCCCCCCCHHHHHCCCCCHHHHHHHHCCCCCCCCCCEEEEEECCC
IMDTGMQEVIRSSSKKDFHEIERFKTYQTEGKLRSTSYVAGILLDLLETDFENGRVYDIK
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEHH
EFL
HCC
>Mature Secondary Structure
MHLSIVTGASKGLGAAMAEGLLQKGHIVHAVSRTKSDISHDRLIQHEADLAQLAETEKWF
CEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RQFSDSVQAHDFASITLINNAGMVTPIKRAGEAEPEELRLHYDLNLLAPVMLSQLFTKWL
HHHHHHHHHCCCEEEEEECCCCCCCCHHHCCCCCHHHEEEEECHHHHHHHHHHHHHHHHH
KPYKGKKSIVNISSGAAKNPYKGWSAYCSSKAGLDMFTKTFGFEQEDESDPVKMISFSPG
CCCCCCCCEEEECCCCCCCCCCCHHHHHCCCCCHHHHHHHHCCCCCCCCCCEEEEEECCC
IMDTGMQEVIRSSSKKDFHEIERFKTYQTEGKLRSTSYVAGILLDLLETDFENGRVYDIK
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEHH
EFL
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 5550 [C]

Specific activity: 66

Km value (mM): 0.26 {methyl-2,3-dehydro-3-hydroxybenzoate}} 0.2 {3-oxocyclohexane-1-carboxylic} 0.3 {2,3-dihydro-2,3-dihydroxybenzoate}} [C]

Substrates: 2,3-Dihydro-2,3-dihydroxybenzoate; Nicotinamide adenine dinucleotide [C]

Specific reaction: 2,3-Dihydro-2,3-dihydroxybenzoate + Nicotinamide adenine dinucleotide <==> 2,3-Dihydroxybenzoate + H+ + Nicotinamide adenine dinucleotide - reduced [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]