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The map label for this gene is pckA

Identifier: 52786921

GI number: 52786921

Start: 3080948

End: 3082531

Strand: Direct

Name: pckA

Synonym: BLi03197

Alternate gene names: 52786921

Gene position: 3080948-3082531 (Clockwise)

Preceding gene: 52786918

Following gene: 52786922

Centisome position: 72.96

GC content: 49.31

Gene sequence:

>1584_bases
ATGAATTCTGTAGATTTAACAACTGACTTACAAACGCTATTAACGGGCGAAAACGTTCAGATGAACTTATCCGTTCCCCA
TTTGGTTGAAAAGATCCTTGAGCGCAAAGAAGGAGTCTTGACATCTTCCGGAGCCGTGCGGGCAACAACCGGTACATACA
CCGGCCGCTCGCCAAAAGACAAATTCATTGTGGAAGAAGCGAGCACAAAGGACAAAATCGATTGGGGCGCAGTAAACCAG
CCGATTTCAGAAGCCGCCTTTGACCGGCTGTACGCAAAAGTGCTCGGCTATTTAAAGGAACGCGATGAATTGTATGTATT
TGAAGGGTTTGCAGGAGCAGACGAAAAATACCGGCTTCCGATTACTGTTGTCAACGAGTTCGCCTGGCACAACCTGTTTG
CAAGACAGTTGTTCATCAGACCTGAAGAACATGATAAAAAACCTGAAGAACAGCCGTTTACGATTCTTTCAGCTCCGCAT
TTCAAAGCCGATCCGCTCGTTGACGGAACCAGATCTGAAACGTTTATCATCGTGTCGTTTGAGAAGCGGACGATCTTAAT
CGGAGGCACCGAATATGCCGGAGAGATGAAAAAATCGATTTTTTCTATTATGAATTTTCTTCTGCCTGAACAAAACATTC
TACCGATGCACTGTTCGGCAAACATTGGCGAGGAAGGCGGAACTGCTCTCTTCTTCGGATTGTCAGGTACAGGCAAAACG
ACGCTTTCCGCCGATCCGAAGCGGAAGCTGATCGGCGACGACGAACACGGCTGGTCCAGCACAGGCGTTTTCAACATTGA
AGGAGGCTGCTACGCCAAATGCATTAACTTAAGCGAAGAAAAAGAACCGCAGATTTACAATGCGATCCGCTTCGGTTCCG
TTCTCGAAAATGTCGTGGTTGACAGCAAAACCGGAGAGCCGGACTATTCCGACTCATTCTACACGGAAAACACTAGAGCG
GCTTACCCGATTGACGCGATTGACAACATCGTCAAGCCGAGCATCGCAGCGCACCCGCAGACCATCGTATTTTTGACCGC
AGATGCTTTCGGCGTGCTTCCTCCGATCAGCAAGTTAACGAAAGAACAAGCCATGTATCATTTCCTGAGCGGATATACAA
GCAAGCTCGCCGGCACCGAACGCGGGATCACCACTCCTGAAGCCACATTCTCGACCTGCTTCGGTTCACCGTTCCTCCCG
CTTCCGGCACATGTGTACGCTGAAATGCTCGGAAGAAAAATCGATGAGCACGGGGTCGGCGTCTTTCTCGTCAATACAGG
CTGGACCGGCGGCGGATACGGAACCGGGGAAAGAATGAGCCTCGCATATACGAGAGCGATGGTTCAGGCCGCAATAGAAG
GAGAACTGGATAAAGCCGAAATGAGGACTGACCGCATTTTCGGACTTCATTCACCTGTCCACGTTCCGGGTGTGCCTGAT
CAAGTGCTTGACCCGGCCAAAACTTGGACAGATGAAAACGAATACGAGGAAAAAGCGATCCACCTGGCGAAAGCATTCAA
ACAGAATTTCAAAAAGTTCTCCAATACGGAAAACATCGAAAAAGCCGGAGGACCGCTCGTTTAA

Upstream 100 bases:

>100_bases
CGAAAAATGAAGAAACGGTATAGACTATAAGAGTAAATGTGTTATACTAATTCTACATTAATGTTATACACATTAAAAAA
CACGCAAAGGAAGGTTTTTT

Downstream 100 bases:

>100_bases
CTCAGCCTGCTTGCATCAGCGCAGATCTTCTGCGCTGATCCAGTTTATCGAAAACCTCTTCGCCTATCAGCGGCCGATGC
CATTTCCTGGGCAATGATCA

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase

Number of amino acids: Translated: 527; Mature: 527

Protein sequence:

>527_residues
MNSVDLTTDLQTLLTGENVQMNLSVPHLVEKILERKEGVLTSSGAVRATTGTYTGRSPKDKFIVEEASTKDKIDWGAVNQ
PISEAAFDRLYAKVLGYLKERDELYVFEGFAGADEKYRLPITVVNEFAWHNLFARQLFIRPEEHDKKPEEQPFTILSAPH
FKADPLVDGTRSETFIIVSFEKRTILIGGTEYAGEMKKSIFSIMNFLLPEQNILPMHCSANIGEEGGTALFFGLSGTGKT
TLSADPKRKLIGDDEHGWSSTGVFNIEGGCYAKCINLSEEKEPQIYNAIRFGSVLENVVVDSKTGEPDYSDSFYTENTRA
AYPIDAIDNIVKPSIAAHPQTIVFLTADAFGVLPPISKLTKEQAMYHFLSGYTSKLAGTERGITTPEATFSTCFGSPFLP
LPAHVYAEMLGRKIDEHGVGVFLVNTGWTGGGYGTGERMSLAYTRAMVQAAIEGELDKAEMRTDRIFGLHSPVHVPGVPD
QVLDPAKTWTDENEYEEKAIHLAKAFKQNFKKFSNTENIEKAGGPLV

Sequences:

>Translated_527_residues
MNSVDLTTDLQTLLTGENVQMNLSVPHLVEKILERKEGVLTSSGAVRATTGTYTGRSPKDKFIVEEASTKDKIDWGAVNQ
PISEAAFDRLYAKVLGYLKERDELYVFEGFAGADEKYRLPITVVNEFAWHNLFARQLFIRPEEHDKKPEEQPFTILSAPH
FKADPLVDGTRSETFIIVSFEKRTILIGGTEYAGEMKKSIFSIMNFLLPEQNILPMHCSANIGEEGGTALFFGLSGTGKT
TLSADPKRKLIGDDEHGWSSTGVFNIEGGCYAKCINLSEEKEPQIYNAIRFGSVLENVVVDSKTGEPDYSDSFYTENTRA
AYPIDAIDNIVKPSIAAHPQTIVFLTADAFGVLPPISKLTKEQAMYHFLSGYTSKLAGTERGITTPEATFSTCFGSPFLP
LPAHVYAEMLGRKIDEHGVGVFLVNTGWTGGGYGTGERMSLAYTRAMVQAAIEGELDKAEMRTDRIFGLHSPVHVPGVPD
QVLDPAKTWTDENEYEEKAIHLAKAFKQNFKKFSNTENIEKAGGPLV
>Mature_527_residues
MNSVDLTTDLQTLLTGENVQMNLSVPHLVEKILERKEGVLTSSGAVRATTGTYTGRSPKDKFIVEEASTKDKIDWGAVNQ
PISEAAFDRLYAKVLGYLKERDELYVFEGFAGADEKYRLPITVVNEFAWHNLFARQLFIRPEEHDKKPEEQPFTILSAPH
FKADPLVDGTRSETFIIVSFEKRTILIGGTEYAGEMKKSIFSIMNFLLPEQNILPMHCSANIGEEGGTALFFGLSGTGKT
TLSADPKRKLIGDDEHGWSSTGVFNIEGGCYAKCINLSEEKEPQIYNAIRFGSVLENVVVDSKTGEPDYSDSFYTENTRA
AYPIDAIDNIVKPSIAAHPQTIVFLTADAFGVLPPISKLTKEQAMYHFLSGYTSKLAGTERGITTPEATFSTCFGSPFLP
LPAHVYAEMLGRKIDEHGVGVFLVNTGWTGGGYGTGERMSLAYTRAMVQAAIEGELDKAEMRTDRIFGLHSPVHVPGVPD
QVLDPAKTWTDENEYEEKAIHLAKAFKQNFKKFSNTENIEKAGGPLV

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family

Homologues:

Organism=Escherichia coli, GI1789807, Length=504, Percent_Identity=49.4047619047619, Blast_Score=469, Evalue=1e-133,
Organism=Saccharomyces cerevisiae, GI6322950, Length=513, Percent_Identity=50.8771929824561, Blast_Score=499, Evalue=1e-142,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): PCKA_BACLD (Q65FV7)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_080333.1
- RefSeq:   YP_092750.1
- ProteinModelPortal:   Q65FV7
- SMR:   Q65FV7
- STRING:   Q65FV7
- EnsemblBacteria:   EBBACT00000057170
- EnsemblBacteria:   EBBACT00000059011
- GeneID:   3027890
- GeneID:   3099051
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi03197
- KEGG:   bli:BL00839
- NMPDR:   fig|279010.5.peg.785
- eggNOG:   COG1866
- GeneTree:   EBGT00050000001183
- HOGENOM:   HBG293387
- OMA:   MFIRPTE
- ProtClustDB:   PRK09344
- BioCyc:   BLIC279010-1:BLI03197-MONOMER
- BioCyc:   BLIC279010:BL00839-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- HAMAP:   MF_00453
- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- Gene3D:   G3DSA:3.90.228.20
- Gene3D:   G3DSA:3.40.449.10
- PIRSF:   PIRSF006294
- TIGRFAMs:   TIGR00224

Pfam domain/function: PF01293 PEPCK_ATP; SSF68923 PEP_carboxykinase_N

EC number: =4.1.1.49

Molecular weight: Translated: 58167; Mature: 58167

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00532 PEPCK_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSVDLTTDLQTLLTGENVQMNLSVPHLVEKILERKEGVLTSSGAVRATTGTYTGRSPKD
CCCCCCHHHHHHHHCCCCEEEECCHHHHHHHHHHHHCCCEECCCCEEEECCCCCCCCCCC
KFIVEEASTKDKIDWGAVNQPISEAAFDRLYAKVLGYLKERDELYVFEGFAGADEKYRLP
CEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEECC
ITVVNEFAWHNLFARQLFIRPEEHDKKPEEQPFTILSAPHFKADPLVDGTRSETFIIVSF
HHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEE
EKRTILIGGTEYAGEMKKSIFSIMNFLLPEQNILPMHCSANIGEEGGTALFFGLSGTGKT
CCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEEECCCCCC
TLSADPKRKLIGDDEHGWSSTGVFNIEGGCYAKCINLSEEKEPQIYNAIRFGSVLENVVV
EECCCCCHHCCCCCCCCCCCCCEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHC
DSKTGEPDYSDSFYTENTRAAYPIDAIDNIVKPSIAAHPQTIVFLTADAFGVLPPISKLT
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCHHHHH
KEQAMYHFLSGYTSKLAGTERGITTPEATFSTCFGSPFLPLPAHVYAEMLGRKIDEHGVG
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCE
VFLVNTGWTGGGYGTGERMSLAYTRAMVQAAIEGELDKAEMRTDRIFGLHSPVHVPGVPD
EEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHEEECCCCCCCCCCCH
QVLDPAKTWTDENEYEEKAIHLAKAFKQNFKKFSNTENIEKAGGPLV
HHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCC
>Mature Secondary Structure
MNSVDLTTDLQTLLTGENVQMNLSVPHLVEKILERKEGVLTSSGAVRATTGTYTGRSPKD
CCCCCCHHHHHHHHCCCCEEEECCHHHHHHHHHHHHCCCEECCCCEEEECCCCCCCCCCC
KFIVEEASTKDKIDWGAVNQPISEAAFDRLYAKVLGYLKERDELYVFEGFAGADEKYRLP
CEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEECC
ITVVNEFAWHNLFARQLFIRPEEHDKKPEEQPFTILSAPHFKADPLVDGTRSETFIIVSF
HHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEE
EKRTILIGGTEYAGEMKKSIFSIMNFLLPEQNILPMHCSANIGEEGGTALFFGLSGTGKT
CCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEEECCCCCC
TLSADPKRKLIGDDEHGWSSTGVFNIEGGCYAKCINLSEEKEPQIYNAIRFGSVLENVVV
EECCCCCHHCCCCCCCCCCCCCEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHC
DSKTGEPDYSDSFYTENTRAAYPIDAIDNIVKPSIAAHPQTIVFLTADAFGVLPPISKLT
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCHHHHH
KEQAMYHFLSGYTSKLAGTERGITTPEATFSTCFGSPFLPLPAHVYAEMLGRKIDEHGVG
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCE
VFLVNTGWTGGGYGTGERMSLAYTRAMVQAAIEGELDKAEMRTDRIFGLHSPVHVPGVPD
EEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHEEECCCCCCCCCCCH
QVLDPAKTWTDENEYEEKAIHLAKAFKQNFKKFSNTENIEKAGGPLV
HHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA