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The map label for this gene is metK [H]

Identifier: 52786920

GI number: 52786920

Start: 3079081

End: 3080283

Strand: Reverse

Name: metK [H]

Synonym: BLi03196

Alternate gene names: 52786920

Gene position: 3080283-3079081 (Counterclockwise)

Preceding gene: 52786923

Following gene: 52786919

Centisome position: 72.95

GC content: 48.38

Gene sequence:

>1203_bases
ATGAGCAAAAACCGTCGGTTATTTACATCAGAGTCAGTTACGGAGGGACATCCGGACAAAATTTGCGACCAGATTTCGGA
TAGTATATTAGACGAAATTTTAAAGAAAGATCCGAATGCCCGCGTTGCCTGTGAAACATCTGTGACAACCGGATTGGTGC
TGGTAAGCGGTGAAATCACGACGTCCACATACGTCGATATTCCGAAAACAGTCCGCGAAACGATTAAAGAAATTGGCTAC
ACACGTGCAAAATACGGTTTTGATGCCGAAACTTGCGCGGTCTTAACGTCTATAGATGAACAGTCTCCAGACATTGCAAT
GGGAGTCGACCAGGCGCTTGAAGCGCGTGAAGGAGCAATGAGCGACGCTGAAATTGAAGCGATCGGAGCCGGAGACCAAG
GTTTAATGTTCGGTTTTGCCTGCAACGAAACAAAGGAACTTATGCCGCTGCCGATTTCGCTTGCCCATAAATTGTCTCGC
CGTTTGACAGAAGTCCGCAAAGAGGAAATTCTGCCGTATCTGCGTCCGGACGGAAAGACACAGGTAACCGTTGAGTATGA
TGAGAACAACAAGCCGATTCGCATCGATACAATCGTCATTTCGACTCAGCATCATCCTGAGATTTCGCTTGAACAAATCC
AGCGCAATCTGAAAGAGCACGTCATCAATCCGGTAGTTCCAAAAGAGCTGATCGATGAAAACACGAAATACTTCATTAAT
CCTACGGGCCGTTTTGTTATCGGCGGACCGCAGGGGGATGCCGGTTTAACAGGGCGCAAAATCATCGTTGACACCTATGG
CGGCTATGCCCGTCACGGAGGCGGAGCGTTTTCCGGCAAGGATGCGACAAAGGTTGACAGATCTGCAGCTTATGCAGCGA
GATATGTAGCGAAAAATATCGTCGCCGCAGGCCTTGCTGATTCCTGTGAAGTACAGCTTGCCTACGCGATCGGTGTCGCT
CAGCCGGTGTCCATTTCAATCGATACATTCGGTACAGGCAAAGCGAGTGAAGAAACATTGATCGAAGTGGTTCGCAAAAA
CTTTGATCTAAGACCTGCCGGCATTATTAAAATGCTCGATCTTCGCCGTCCGATTTACAAACAAACGGCAGCTTACGGAC
ATTTCGGCCGCCTCGATCTCGATCTGCCTTGGGAGCGCACAGACAAAGCAGATCAGCTGAAGAAAGATGCATTAGGAGAA
TAA

Upstream 100 bases:

>100_bases
AGAGCGTATGAAGCCCGAGTATCGTCATATAATCAAGAGGGACGAGTTCCGTATCATACTTAAGATCACGGCTGATCTTC
ATATACAGGAGGAAACATAA

Downstream 100 bases:

>100_bases
CAACCGCATAGCCGCTTTAATAAGCGGCTTTGTTTTTCGATGGCAAAATGCGGGCGGATGTTGAAACTTGAAAAATATCC
AGATTTAATATTGATGTGAT

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MSKNRRLFTSESVTEGHPDKICDQISDSILDEILKKDPNARVACETSVTTGLVLVSGEITTSTYVDIPKTVRETIKEIGY
TRAKYGFDAETCAVLTSIDEQSPDIAMGVDQALEAREGAMSDAEIEAIGAGDQGLMFGFACNETKELMPLPISLAHKLSR
RLTEVRKEEILPYLRPDGKTQVTVEYDENNKPIRIDTIVISTQHHPEISLEQIQRNLKEHVINPVVPKELIDENTKYFIN
PTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDATKVDRSAAYAARYVAKNIVAAGLADSCEVQLAYAIGVA
QPVSISIDTFGTGKASEETLIEVVRKNFDLRPAGIIKMLDLRRPIYKQTAAYGHFGRLDLDLPWERTDKADQLKKDALGE

Sequences:

>Translated_400_residues
MSKNRRLFTSESVTEGHPDKICDQISDSILDEILKKDPNARVACETSVTTGLVLVSGEITTSTYVDIPKTVRETIKEIGY
TRAKYGFDAETCAVLTSIDEQSPDIAMGVDQALEAREGAMSDAEIEAIGAGDQGLMFGFACNETKELMPLPISLAHKLSR
RLTEVRKEEILPYLRPDGKTQVTVEYDENNKPIRIDTIVISTQHHPEISLEQIQRNLKEHVINPVVPKELIDENTKYFIN
PTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDATKVDRSAAYAARYVAKNIVAAGLADSCEVQLAYAIGVA
QPVSISIDTFGTGKASEETLIEVVRKNFDLRPAGIIKMLDLRRPIYKQTAAYGHFGRLDLDLPWERTDKADQLKKDALGE
>Mature_399_residues
SKNRRLFTSESVTEGHPDKICDQISDSILDEILKKDPNARVACETSVTTGLVLVSGEITTSTYVDIPKTVRETIKEIGYT
RAKYGFDAETCAVLTSIDEQSPDIAMGVDQALEAREGAMSDAEIEAIGAGDQGLMFGFACNETKELMPLPISLAHKLSRR
LTEVRKEEILPYLRPDGKTQVTVEYDENNKPIRIDTIVISTQHHPEISLEQIQRNLKEHVINPVVPKELIDENTKYFINP
TGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDATKVDRSAAYAARYVAKNIVAAGLADSCEVQLAYAIGVAQ
PVSISIDTFGTGKASEETLIEVVRKNFDLRPAGIIKMLDLRRPIYKQTAAYGHFGRLDLDLPWERTDKADQLKKDALGE

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557737, Length=388, Percent_Identity=59.7938144329897, Blast_Score=452, Evalue=1e-127,
Organism=Homo sapiens, GI5174529, Length=382, Percent_Identity=59.9476439790576, Blast_Score=444, Evalue=1e-124,
Organism=Escherichia coli, GI1789311, Length=395, Percent_Identity=63.2911392405063, Blast_Score=507, Evalue=1e-145,
Organism=Caenorhabditis elegans, GI25145633, Length=392, Percent_Identity=56.3775510204082, Blast_Score=431, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17538498, Length=389, Percent_Identity=56.0411311053985, Blast_Score=429, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17538494, Length=389, Percent_Identity=55.2699228791774, Blast_Score=424, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17551082, Length=386, Percent_Identity=53.1088082901554, Blast_Score=413, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI32565905, Length=389, Percent_Identity=50.6426735218509, Blast_Score=369, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI32565911, Length=338, Percent_Identity=54.7337278106509, Blast_Score=356, Evalue=1e-98,
Organism=Caenorhabditis elegans, GI25145635, Length=341, Percent_Identity=54.2521994134897, Blast_Score=353, Evalue=1e-97,
Organism=Caenorhabditis elegans, GI32565903, Length=311, Percent_Identity=54.3408360128617, Blast_Score=323, Evalue=7e-89,
Organism=Caenorhabditis elegans, GI32565909, Length=87, Percent_Identity=55.1724137931034, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI32565907, Length=44, Percent_Identity=68.1818181818182, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6320710, Length=393, Percent_Identity=56.4885496183206, Blast_Score=441, Evalue=1e-125,
Organism=Saccharomyces cerevisiae, GI6323209, Length=383, Percent_Identity=57.4412532637076, Blast_Score=441, Evalue=1e-125,
Organism=Drosophila melanogaster, GI45552159, Length=382, Percent_Identity=57.0680628272251, Blast_Score=417, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24580529, Length=382, Percent_Identity=57.0680628272251, Blast_Score=417, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24580537, Length=382, Percent_Identity=57.0680628272251, Blast_Score=417, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24580533, Length=382, Percent_Identity=57.0680628272251, Blast_Score=417, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24580535, Length=382, Percent_Identity=57.0680628272251, Blast_Score=417, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24580531, Length=382, Percent_Identity=57.0680628272251, Blast_Score=417, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24580527, Length=382, Percent_Identity=56.020942408377, Blast_Score=410, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24580525, Length=382, Percent_Identity=56.020942408377, Blast_Score=410, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24580541, Length=370, Percent_Identity=54.8648648648649, Blast_Score=388, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580539, Length=127, Percent_Identity=55.1181102362205, Blast_Score=137, Evalue=1e-32,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 43888; Mature: 43757

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKNRRLFTSESVTEGHPDKICDQISDSILDEILKKDPNARVACETSVTTGLVLVSGEIT
CCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEEECCCC
TSTYVDIPKTVRETIKEIGYTRAKYGFDAETCAVLTSIDEQSPDIAMGVDQALEAREGAM
CCEEECCCHHHHHHHHHCCCCHHHCCCCHHHHHHHHHCCCCCCCHHHCHHHHHHHHHCCC
SDAEIEAIGAGDQGLMFGFACNETKELMPLPISLAHKLSRRLTEVRKEEILPYLRPDGKT
CCCCEEEECCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCE
QVTVEYDENNKPIRIDTIVISTQHHPEISLEQIQRNLKEHVINPVVPKELIDENTKYFIN
EEEEEECCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCHHHHCCCCEEEEC
PTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDATKVDRSAAYAARYVAKNI
CCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
VAAGLADSCEVQLAYAIGVAQPVSISIDTFGTGKASEETLIEVVRKNFDLRPAGIIKMLD
HHHCCCCCCCEEEEEEECCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHH
LRRPIYKQTAAYGHFGRLDLDLPWERTDKADQLKKDALGE
HCCHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure 
SKNRRLFTSESVTEGHPDKICDQISDSILDEILKKDPNARVACETSVTTGLVLVSGEIT
CCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEEECCCC
TSTYVDIPKTVRETIKEIGYTRAKYGFDAETCAVLTSIDEQSPDIAMGVDQALEAREGAM
CCEEECCCHHHHHHHHHCCCCHHHCCCCHHHHHHHHHCCCCCCCHHHCHHHHHHHHHCCC
SDAEIEAIGAGDQGLMFGFACNETKELMPLPISLAHKLSRRLTEVRKEEILPYLRPDGKT
CCCCEEEECCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCE
QVTVEYDENNKPIRIDTIVISTQHHPEISLEQIQRNLKEHVINPVVPKELIDENTKYFIN
EEEEEECCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCHHHHCCCCEEEEC
PTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDATKVDRSAAYAARYVAKNI
CCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
VAAGLADSCEVQLAYAIGVAQPVSISIDTFGTGKASEETLIEVVRKNFDLRPAGIIKMLD
HHHCCCCCCCEEEEEEECCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHH
LRRPIYKQTAAYGHFGRLDLDLPWERTDKADQLKKDALGE
HCCHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA