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The map label for this gene is asnB [H]

Identifier: 52786919

GI number: 52786919

Start: 3076953

End: 3078851

Strand: Reverse

Name: asnB [H]

Synonym: BLi03195

Alternate gene names: 52786919

Gene position: 3078851-3076953 (Counterclockwise)

Preceding gene: 52786920

Following gene: 52786915

Centisome position: 72.91

GC content: 45.55

Gene sequence:

>1899_bases
ATGTGTGGATTTGTAGGGGTATTCAATCATCGCCCATCATCCGAGACAGCAGCACAGGAAGAACTGATTAAACAAATGAA
TGAAATGATTGTACACCGCGGTCCGGACGATGATGGGTACTATCATGATGAGCATGTAGGCTTCGGATTTAGAAGGCTCA
GCATCATCGATGTGGAAAATGGAGGACAGCCTCTGTCTTATGAAGACGACTCCTATTGGATTATTTTTAATGGAGAAATC
TATAACTATATAGAACTGAAAGACGAACTTCTTTCTAAAGGATACGAGTTCAAAACCGATTCAGATACGGAAGTTTTGCT
TGCGACTTACCGCCACTACAAACAGGAAGCCGCTTCAAAGCTGCGCGGTATGTTTGCTTTTTTAATCTGGGATAAAAAAG
AGCAGCTTCTATACGGAGCGCGCGATCCGTTCGGCATTAAGCCGCTCTATTTTACGAAAACGGACGGCCACGTTTATTTT
GCGTCAGAAAGAAAGAGCCTGATGGCTGTCGATGCTGACTTGGAATTGAATGAAAAAGCGCTTCAGCAGTATACATCGTT
CCAATTCGTTCCGGAGCCTGAGACGCTGGACAAAAAGGTGCAAAAAGTAGAGTCGGGCCATCAGTTTACCGTCCGCCCGG
GTGAGGATATTCAATTTAAAACATACTGGAAAGTTCAATTCAAGCCGGTTCAGACAGAAGAAGACAAGCTTGTTCAGGAA
GTAAGAGACGCGATCTTTGATTCTGTAAAAGTTCATATGCGAAGCGATGTGCCTGTAGGTTCCTTCCTTTCCGGGGGCAT
TGACTCGTCCTTTATCGTGTCAGTGGCGAAGCAATTCCATCCCAACTTAAAGACGTTCTCTGTCGGCTTTGAACATGAAG
GCTTCAGCGAAGTTGACGTTGCGAAAGAAACCGCTGATAAGGTCGGCGTGGAAAACTTCAGCGCGATCATTTCCCCTGAG
GAATATATGAACGAGCTTCCAAAGATCGTCTGGCATCTGGATGATCCTCTGGCAGACCCTGCTGCGATTCCTTTGTATTT
TGTCGCGAAGGAAGCGAAGAAGCAAGTTACGGTTGTTTTGTCAGGAGAAGGGGCAGATGAGCTGTTCGGCGGTTACAATA
TTTACCGAGAGCCGCTTTCATTAAAGCCGTTTGAGCGGATCCCGTCCCCGCTGAAAAAGATGCTTCTGCGCGTAGCTTCA
GCGATGCCGGAAGGAATGAAAGGGAAAAGCTTTTTAATGAGGGGCTGTACTCCTCTTGAAGATAGATATATCGGCAATGC
AAAGATTTTTGAAGAAGGCATGAAATCAAAACTTCTTCGCCAATATGACAGCAACCTGTCTTACTGTGACGTGACAAAGC
CATATTTTGAAGAAAGCAGGTCCTACAGCGAAATCAATAAAATGCAGTATGTCGATATCCACACATGGCTGCGCGGCGAT
ATTTTGCTGAAGGCCGACAAAATGACGATGGCCAATTCGCTGGAGCTTCGCGTTCCGTTTTTGGACAAAGTCGTATTTGA
AGCGGCTTCCAAAATTCCCGAAGAGCTGAAAACGAAAAACGGCACGACAAAATATCTTCTTAGAAAAGCGGCGGAAGGCA
TCGTTCCAGAACACGTGCTAAACCGCAAAAAACTAGGGTTCCCTGTGCCAATCCGCCACTGGCTGAAAAACGAAATGCAT
GACTGGGCCGTGAACATCATTAAAGAGAGCGAGACAGATGCATATATCCATAAAGATTACGTGCTTCAGCTTCTTGAAGA
CCACTGCGCCAACAAAGCGGATAACAGCCGCAAAATCTGGACGGTTCTCATCTTTATGATCTGGCACAGCATTTTCGTTG
AAAAACGCTTTGTACCGGAAGAGCTGAACCATCAGCCAAAAGAGGTCATTATTGTTTAA

Upstream 100 bases:

>100_bases
CATTTTTCAAGTTTCTTGAAACGTTTTTCTTTAAACACTTGTCTAATCAAGAAGCAGACTTTGATTTCTGCACGAAATTT
AAAAAATCGGAGGCAATACT

Downstream 100 bases:

>100_bases
GCCAAAAAAAGAAGGTTTTCGATTAACCGAAAACCTTCTTTTTTTATGCCGGTTTTTCTGCTGATAAATCTGCAAGCACG
CTTTCACATTCGGAGAGGAG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 632; Mature: 632

Protein sequence:

>632_residues
MCGFVGVFNHRPSSETAAQEELIKQMNEMIVHRGPDDDGYYHDEHVGFGFRRLSIIDVENGGQPLSYEDDSYWIIFNGEI
YNYIELKDELLSKGYEFKTDSDTEVLLATYRHYKQEAASKLRGMFAFLIWDKKEQLLYGARDPFGIKPLYFTKTDGHVYF
ASERKSLMAVDADLELNEKALQQYTSFQFVPEPETLDKKVQKVESGHQFTVRPGEDIQFKTYWKVQFKPVQTEEDKLVQE
VRDAIFDSVKVHMRSDVPVGSFLSGGIDSSFIVSVAKQFHPNLKTFSVGFEHEGFSEVDVAKETADKVGVENFSAIISPE
EYMNELPKIVWHLDDPLADPAAIPLYFVAKEAKKQVTVVLSGEGADELFGGYNIYREPLSLKPFERIPSPLKKMLLRVAS
AMPEGMKGKSFLMRGCTPLEDRYIGNAKIFEEGMKSKLLRQYDSNLSYCDVTKPYFEESRSYSEINKMQYVDIHTWLRGD
ILLKADKMTMANSLELRVPFLDKVVFEAASKIPEELKTKNGTTKYLLRKAAEGIVPEHVLNRKKLGFPVPIRHWLKNEMH
DWAVNIIKESETDAYIHKDYVLQLLEDHCANKADNSRKIWTVLIFMIWHSIFVEKRFVPEELNHQPKEVIIV

Sequences:

>Translated_632_residues
MCGFVGVFNHRPSSETAAQEELIKQMNEMIVHRGPDDDGYYHDEHVGFGFRRLSIIDVENGGQPLSYEDDSYWIIFNGEI
YNYIELKDELLSKGYEFKTDSDTEVLLATYRHYKQEAASKLRGMFAFLIWDKKEQLLYGARDPFGIKPLYFTKTDGHVYF
ASERKSLMAVDADLELNEKALQQYTSFQFVPEPETLDKKVQKVESGHQFTVRPGEDIQFKTYWKVQFKPVQTEEDKLVQE
VRDAIFDSVKVHMRSDVPVGSFLSGGIDSSFIVSVAKQFHPNLKTFSVGFEHEGFSEVDVAKETADKVGVENFSAIISPE
EYMNELPKIVWHLDDPLADPAAIPLYFVAKEAKKQVTVVLSGEGADELFGGYNIYREPLSLKPFERIPSPLKKMLLRVAS
AMPEGMKGKSFLMRGCTPLEDRYIGNAKIFEEGMKSKLLRQYDSNLSYCDVTKPYFEESRSYSEINKMQYVDIHTWLRGD
ILLKADKMTMANSLELRVPFLDKVVFEAASKIPEELKTKNGTTKYLLRKAAEGIVPEHVLNRKKLGFPVPIRHWLKNEMH
DWAVNIIKESETDAYIHKDYVLQLLEDHCANKADNSRKIWTVLIFMIWHSIFVEKRFVPEELNHQPKEVIIV
>Mature_632_residues
MCGFVGVFNHRPSSETAAQEELIKQMNEMIVHRGPDDDGYYHDEHVGFGFRRLSIIDVENGGQPLSYEDDSYWIIFNGEI
YNYIELKDELLSKGYEFKTDSDTEVLLATYRHYKQEAASKLRGMFAFLIWDKKEQLLYGARDPFGIKPLYFTKTDGHVYF
ASERKSLMAVDADLELNEKALQQYTSFQFVPEPETLDKKVQKVESGHQFTVRPGEDIQFKTYWKVQFKPVQTEEDKLVQE
VRDAIFDSVKVHMRSDVPVGSFLSGGIDSSFIVSVAKQFHPNLKTFSVGFEHEGFSEVDVAKETADKVGVENFSAIISPE
EYMNELPKIVWHLDDPLADPAAIPLYFVAKEAKKQVTVVLSGEGADELFGGYNIYREPLSLKPFERIPSPLKKMLLRVAS
AMPEGMKGKSFLMRGCTPLEDRYIGNAKIFEEGMKSKLLRQYDSNLSYCDVTKPYFEESRSYSEINKMQYVDIHTWLRGD
ILLKADKMTMANSLELRVPFLDKVVFEAASKIPEELKTKNGTTKYLLRKAAEGIVPEHVLNRKKLGFPVPIRHWLKNEMH
DWAVNIIKESETDAYIHKDYVLQLLEDHCANKADNSRKIWTVLIFMIWHSIFVEKRFVPEELNHQPKEVIIV

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=414, Percent_Identity=28.743961352657, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI168229252, Length=414, Percent_Identity=28.743961352657, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI168229250, Length=414, Percent_Identity=28.743961352657, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI296010848, Length=385, Percent_Identity=29.3506493506493, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI296010852, Length=313, Percent_Identity=28.1150159744409, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI296010850, Length=313, Percent_Identity=28.1150159744409, Blast_Score=97, Evalue=3e-20,
Organism=Escherichia coli, GI1786889, Length=392, Percent_Identity=30.6122448979592, Blast_Score=180, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI71993933, Length=397, Percent_Identity=31.9899244332494, Blast_Score=171, Evalue=9e-43,
Organism=Caenorhabditis elegans, GI25147557, Length=397, Percent_Identity=31.7380352644836, Blast_Score=171, Evalue=9e-43,
Organism=Caenorhabditis elegans, GI17560178, Length=403, Percent_Identity=29.2803970223325, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI25147560, Length=320, Percent_Identity=30, Blast_Score=122, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6325403, Length=423, Percent_Identity=29.0780141843972, Blast_Score=160, Evalue=7e-40,
Organism=Saccharomyces cerevisiae, GI6321563, Length=422, Percent_Identity=27.7251184834123, Blast_Score=149, Evalue=1e-36,
Organism=Drosophila melanogaster, GI45553209, Length=410, Percent_Identity=32.1951219512195, Blast_Score=176, Evalue=6e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 72828; Mature: 72828

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGFVGVFNHRPSSETAAQEELIKQMNEMIVHRGPDDDGYYHDEHVGFGFRRLSIIDVEN
CCCEEECCCCCCCCHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCEEEEEEEEECC
GGQPLSYEDDSYWIIFNGEIYNYIELKDELLSKGYEFKTDSDTEVLLATYRHYKQEAASK
CCCCCCCCCCCEEEEECCCEEEHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LRGMFAFLIWDKKEQLLYGARDPFGIKPLYFTKTDGHVYFASERKSLMAVDADLELNEKA
HHHHEEEEEECCHHHHHCCCCCCCCCCEEEEEECCCEEEEECCCCEEEEEECCCCCCHHH
LQQYTSFQFVPEPETLDKKVQKVESGHQFTVRPGEDIQFKTYWKVQFKPVQTEEDKLVQE
HHHHHCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCEEEEEEEEEEEECCCHHHHHHHH
VRDAIFDSVKVHMRSDVPVGSFLSGGIDSSFIVSVAKQFHPNLKTFSVGFEHEGFSEVDV
HHHHHHHHHHHEECCCCCHHHHHHCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHH
AKETADKVGVENFSAIISPEEYMNELPKIVWHLDDPLADPAAIPLYFVAKEAKKQVTVVL
HHHHHHHHCHHHHHHEECCHHHHHHHHHHEEECCCCCCCCCCCHHHEEEHHCCCEEEEEE
SGEGADELFGGYNIYREPLSLKPFERIPSPLKKMLLRVASAMPEGMKGKSFLMRGCTPLE
ECCCHHHHHCCCHHHCCCCCCCCHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCHH
DRYIGNAKIFEEGMKSKLLRQYDSNLSYCDVTKPYFEESRSYSEINKMQYVDIHTWLRGD
HHCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHCCCHHHHHHEEEEEEHHEECCC
ILLKADKMTMANSLELRVPFLDKVVFEAASKIPEELKTKNGTTKYLLRKAAEGIVPEHVL
EEEEECCEECCCCCEEECCHHHHHHHHHHHHCHHHHHCCCCHHHHHHHHHHHCCCCHHHH
NRKKLGFPVPIRHWLKNEMHDWAVNIIKESETDAYIHKDYVLQLLEDHCANKADNSRKIW
CHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHH
TVLIFMIWHSIFVEKRFVPEELNHQPKEVIIV
HHHHHHHHHHHHHHHCCCCHHHCCCCCEEEEC
>Mature Secondary Structure
MCGFVGVFNHRPSSETAAQEELIKQMNEMIVHRGPDDDGYYHDEHVGFGFRRLSIIDVEN
CCCEEECCCCCCCCHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCEEEEEEEEECC
GGQPLSYEDDSYWIIFNGEIYNYIELKDELLSKGYEFKTDSDTEVLLATYRHYKQEAASK
CCCCCCCCCCCEEEEECCCEEEHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LRGMFAFLIWDKKEQLLYGARDPFGIKPLYFTKTDGHVYFASERKSLMAVDADLELNEKA
HHHHEEEEEECCHHHHHCCCCCCCCCCEEEEEECCCEEEEECCCCEEEEEECCCCCCHHH
LQQYTSFQFVPEPETLDKKVQKVESGHQFTVRPGEDIQFKTYWKVQFKPVQTEEDKLVQE
HHHHHCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCEEEEEEEEEEEECCCHHHHHHHH
VRDAIFDSVKVHMRSDVPVGSFLSGGIDSSFIVSVAKQFHPNLKTFSVGFEHEGFSEVDV
HHHHHHHHHHHEECCCCCHHHHHHCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHH
AKETADKVGVENFSAIISPEEYMNELPKIVWHLDDPLADPAAIPLYFVAKEAKKQVTVVL
HHHHHHHHCHHHHHHEECCHHHHHHHHHHEEECCCCCCCCCCCHHHEEEHHCCCEEEEEE
SGEGADELFGGYNIYREPLSLKPFERIPSPLKKMLLRVASAMPEGMKGKSFLMRGCTPLE
ECCCHHHHHCCCHHHCCCCCCCCHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCHH
DRYIGNAKIFEEGMKSKLLRQYDSNLSYCDVTKPYFEESRSYSEINKMQYVDIHTWLRGD
HHCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHCCCHHHHHHEEEEEEHHEECCC
ILLKADKMTMANSLELRVPFLDKVVFEAASKIPEELKTKNGTTKYLLRKAAEGIVPEHVL
EEEEECCEECCCCCEEECCHHHHHHHHHHHHCHHHHHCCCCHHHHHHHHHHHCCCCHHHH
NRKKLGFPVPIRHWLKNEMHDWAVNIIKESETDAYIHKDYVLQLLEDHCANKADNSRKIW
CHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHH
TVLIFMIWHSIFVEKRFVPEELNHQPKEVIIV
HHHHHHHHHHHHHHHCCCCHHHCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]