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The map label for this gene is ytpR [H]

Identifier: 52786858

GI number: 52786858

Start: 3016369

End: 3016974

Strand: Reverse

Name: ytpR [H]

Synonym: BLi03133

Alternate gene names: 52786858

Gene position: 3016974-3016369 (Counterclockwise)

Preceding gene: 52786859

Following gene: 52786857

Centisome position: 71.45

GC content: 46.86

Gene sequence:

>606_bases
ATGAACGCATTTTATAATCCAGAAGGCGTAGGCGATACGCTGCTCATCTCATTGAACGATATTCCACGTGAAGAGATCGC
TTTTGAGCGGTACGGAGACGTCGTGAAAATTTTTCATAAAGACACAAAGGAAACAGCAGGCTATAACATTTTTAATGCCT
CCTCATATTTGAGCATTGAGGAAAGCGGAGCTATTGCGCTTTCCGAATCCTTTGTCCAGGATGTGAATGAAATTCTGGTC
CGCAACGGTTTGAAAGACGAGCTGACCGTCGACCTTTCTCCGAAGTTTGTGGTCGGATATGTTGAAGAAAAAGAAAAGCA
TCCGAATGCCGATAAATTAAGTGTTTGCAAAGTGAATGTAGGGAGCGAGACGCTGCAAATCGTCTGCGGAGCTCCCAATG
TTGAAGCCGGCCAAAAAGTCGTCGTTGCCAAAGTCGGCGCCGTGATGCCTAGCGGTATGATCATTAAAGATGCGGAACTT
CGCGGAGTGGCGTCAAGCGGCATGATTTGTTCTGCCAAGGAGCTGAATCTCCCAGACGCGCCGAAGGAAAAAGGCATTCT
TGTTCTTGACGACGGCCGTAAAGCAGGAGAAGCCTTTACATTTTAA

Upstream 100 bases:

>100_bases
CACTGCGCTTTCTTTCCTGTATGAGGATGGAAAGCTTGAACCGATCTTTATTCTGGCTAAAAACCGCCCTAAAAAGAAAT
GATAGAAAGGAATTTTTCTC

Downstream 100 bases:

>100_bases
CGGCATAAAATGAACAAAACTTTCGAATAGATGTAAAAAGAAGCCGCTGGGTAGACAAACTTGGCGGCTTTTTTATTCGG
AGGGCGGACGCTTGGAGAAG

Product: YtpR

Products: AMP; diphosphate; L-phenylalanyl-tRNAPhe [C]

Alternate protein names: Partial phenylalanyl-tRNA synthetase; Partial PheRS; pFRS [H]

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MNAFYNPEGVGDTLLISLNDIPREEIAFERYGDVVKIFHKDTKETAGYNIFNASSYLSIEESGAIALSESFVQDVNEILV
RNGLKDELTVDLSPKFVVGYVEEKEKHPNADKLSVCKVNVGSETLQIVCGAPNVEAGQKVVVAKVGAVMPSGMIIKDAEL
RGVASSGMICSAKELNLPDAPKEKGILVLDDGRKAGEAFTF

Sequences:

>Translated_201_residues
MNAFYNPEGVGDTLLISLNDIPREEIAFERYGDVVKIFHKDTKETAGYNIFNASSYLSIEESGAIALSESFVQDVNEILV
RNGLKDELTVDLSPKFVVGYVEEKEKHPNADKLSVCKVNVGSETLQIVCGAPNVEAGQKVVVAKVGAVMPSGMIIKDAEL
RGVASSGMICSAKELNLPDAPKEKGILVLDDGRKAGEAFTF
>Mature_201_residues
MNAFYNPEGVGDTLLISLNDIPREEIAFERYGDVVKIFHKDTKETAGYNIFNASSYLSIEESGAIALSESFVQDVNEILV
RNGLKDELTVDLSPKFVVGYVEEKEKHPNADKLSVCKVNVGSETLQIVCGAPNVEAGQKVVVAKVGAVMPSGMIIKDAEL
RGVASSGMICSAKELNLPDAPKEKGILVLDDGRKAGEAFTF

Specific function: Unknown

COG id: COG0073

COG function: function code R; EMAP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788006, Length=75, Percent_Identity=57.3333333333333, Blast_Score=85, Evalue=3e-18,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR002547 [H]

Pfam domain/function: PF01588 tRNA_bind [H]

EC number: 6.1.1.20 [C]

Molecular weight: Translated: 21669; Mature: 21669

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAFYNPEGVGDTLLISLNDIPREEIAFERYGDVVKIFHKDTKETAGYNIFNASSYLSIE
CCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCEEEEEECCCHHHCCCEEECCCCEEEEE
ESGAIALSESFVQDVNEILVRNGLKDELTVDLSPKFVVGYVEEKEKHPNADKLSVCKVNV
CCCCEEEHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEEHHCCCCCCCCEEEEEEEC
GSETLQIVCGAPNVEAGQKVVVAKVGAVMPSGMIIKDAELRGVASSGMICSAKELNLPDA
CCCEEEEEECCCCCCCCCEEEEEECCCCCCCCEEEECCCCCCCCCCCEEEEHHCCCCCCC
PKEKGILVLDDGRKAGEAFTF
CCCCCEEEEECCCCCCCCCCC
>Mature Secondary Structure
MNAFYNPEGVGDTLLISLNDIPREEIAFERYGDVVKIFHKDTKETAGYNIFNASSYLSIE
CCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCEEEEEECCCHHHCCCEEECCCCEEEEE
ESGAIALSESFVQDVNEILVRNGLKDELTVDLSPKFVVGYVEEKEKHPNADKLSVCKVNV
CCCCEEEHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEEHHCCCCCCCCEEEEEEEC
GSETLQIVCGAPNVEAGQKVVVAKVGAVMPSGMIIKDAELRGVASSGMICSAKELNLPDA
CCCEEEEEECCCCCCCCCEEEEEECCCCCCCCEEEECCCCCCCCCCCEEEEHHCCCCCCC
PKEKGILVLDDGRKAGEAFTF
CCCCCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.125 {2'-deoxyadenosine} 0.2 {2-chloroadenosine} 0.11 {2-chloroadenosine} 0.009 {ATP}} [C]

Substrates: ATP; @PHEN01.txt*phenylalanine!; tRNAPhe [C]

Specific reaction: ATP + @PHEN01.txt*phenylalanine! + tRNAPhe = AMP + diphosphate + L-phenylalanyl-tRNAPhe [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 3'-Deoxyadenosine 5'-triphosphate; 3'-Methyl adenosine 5'-triphosphate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]