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The map label for this gene is nifS [H]

Identifier: 52786647

GI number: 52786647

Start: 2802696

End: 2803874

Strand: Direct

Name: nifS [H]

Synonym: BLi02915

Alternate gene names: 52786647

Gene position: 2802696-2803874 (Clockwise)

Preceding gene: 52786639

Following gene: 52786648

Centisome position: 66.37

GC content: 49.19

Gene sequence:

>1179_bases
GTGATTTATCTTGATTATGCAGCGACGACACCGGCATCAAAAGAAGCCTTGGACGCGTTTCAAAAACTTAGCACAGACGT
ATACGGAAATTCGAGCAGTCTGCACGATGCAGGAGGGAAAGCCGAAAACATTTTGCGCTGCTGCCGCCAAAGGATGGCAG
AACTTTTATCCGGAGAAGAAGAAGGCGTTTATTTTACGAGCGGAGGCACCGAAGCCAACTTTTTAGCGATTCACTCCATC
TTAAATGGACTTCCCGCCGGGAAAAAGCGCTTTTTAATGACAGAAATGGAGCATCAGTCGATCCATATTTTGAAACGGCA
TCTCGAGATGCTGGGATTTGATGTCGACTTGATGAAGCCGAATGCTTCAGGCATCATTACAACGGATATACTTGAACGGC
ACCTCCGCCCGGATACGGGTCTGGTTTCGATTCAGCATGCCAATTCAGAAACAGGCGTCATCCAGCCTCTGGCAAAGCTC
TCGGCTTACCTGCATAAGCGGGACGTGCTCTTGCACAGCGACTGCGTACAGACATTTGCCAAGATTCCGGTCCGCATTGA
TGAGCTCGGCGCTGATGCCTTATCGGTCTCAAGCCACAAAGTATACGGCCCAAAAGGAGTTGGGGCTGTCTATATTCGGC
CGGGTGTAAACTGGAAACCCGTCTACCCGGGCACTTTGCATGAAAACGGCTTTAAAAGCGGCACCGTCAATGTCCCTGGA
ATCGGCGCTTTCATTACCGCTGCAGATTCGACAATAAACCGCATGGATGCCGCGCATCTCAAATTCCAGGAGCTGCGCGC
AGGTTTTCTGGAAGGAGTAAAAGACCGGAAGCTTCCCGTCTCCCTTGCTGTGCCCGATTCGGCAAATTCGCATGTTCTGC
CGCATATTATAGGTTGTTTTTTTCATTCCTTTGAAGGACAATATACTATGCTGGAATGTAATCGGAGAGGAATTTGCATT
TCTACCGGAAGCGCGTGTTCAGCGGGCTATCATGAGCCTTCGAATACGATGAAAGCCCTTTTGATCCCTAGGGAGCTCGC
GCTTCAATTCGTCCGGATTTCATTCGGATGCGATACGACAAGCGAACATGTTGATAGGCTGTTGGACGTTCTTGAAGATA
TGATGAATGAGAAAGGAGACCATCGGATTGACAGACGGATTAAAACTTACGGGAGCTGA

Upstream 100 bases:

>100_bases
GTCGACTCCTCCTGTAAATTTACATGTGTCTTGACACCTATATTTACACATCGATAAAATAAACTCAAGTATTTTTTATA
AAGAATGGGAGACGAGCGCC

Downstream 100 bases:

>100_bases
GCGGCGCCAGCAGCTGCTGGCTTGGCTGAAGGAGTCAGAGGCTCCGCTTACAGGAAGCGAGCTGGCCAAAAGAGCATCTG
TTTCACGGCAGGTAATAGTA

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MIYLDYAATTPASKEALDAFQKLSTDVYGNSSSLHDAGGKAENILRCCRQRMAELLSGEEEGVYFTSGGTEANFLAIHSI
LNGLPAGKKRFLMTEMEHQSIHILKRHLEMLGFDVDLMKPNASGIITTDILERHLRPDTGLVSIQHANSETGVIQPLAKL
SAYLHKRDVLLHSDCVQTFAKIPVRIDELGADALSVSSHKVYGPKGVGAVYIRPGVNWKPVYPGTLHENGFKSGTVNVPG
IGAFITAADSTINRMDAAHLKFQELRAGFLEGVKDRKLPVSLAVPDSANSHVLPHIIGCFFHSFEGQYTMLECNRRGICI
STGSACSAGYHEPSNTMKALLIPRELALQFVRISFGCDTTSEHVDRLLDVLEDMMNEKGDHRIDRRIKTYGS

Sequences:

>Translated_392_residues
MIYLDYAATTPASKEALDAFQKLSTDVYGNSSSLHDAGGKAENILRCCRQRMAELLSGEEEGVYFTSGGTEANFLAIHSI
LNGLPAGKKRFLMTEMEHQSIHILKRHLEMLGFDVDLMKPNASGIITTDILERHLRPDTGLVSIQHANSETGVIQPLAKL
SAYLHKRDVLLHSDCVQTFAKIPVRIDELGADALSVSSHKVYGPKGVGAVYIRPGVNWKPVYPGTLHENGFKSGTVNVPG
IGAFITAADSTINRMDAAHLKFQELRAGFLEGVKDRKLPVSLAVPDSANSHVLPHIIGCFFHSFEGQYTMLECNRRGICI
STGSACSAGYHEPSNTMKALLIPRELALQFVRISFGCDTTSEHVDRLLDVLEDMMNEKGDHRIDRRIKTYGS
>Mature_392_residues
MIYLDYAATTPASKEALDAFQKLSTDVYGNSSSLHDAGGKAENILRCCRQRMAELLSGEEEGVYFTSGGTEANFLAIHSI
LNGLPAGKKRFLMTEMEHQSIHILKRHLEMLGFDVDLMKPNASGIITTDILERHLRPDTGLVSIQHANSETGVIQPLAKL
SAYLHKRDVLLHSDCVQTFAKIPVRIDELGADALSVSSHKVYGPKGVGAVYIRPGVNWKPVYPGTLHENGFKSGTVNVPG
IGAFITAADSTINRMDAAHLKFQELRAGFLEGVKDRKLPVSLAVPDSANSHVLPHIIGCFFHSFEGQYTMLECNRRGICI
STGSACSAGYHEPSNTMKALLIPRELALQFVRISFGCDTTSEHVDRLLDVLEDMMNEKGDHRIDRRIKTYGS

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine. Seems to be required for NAD biosynthesis [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=367, Percent_Identity=33.7874659400545, Blast_Score=204, Evalue=2e-52,
Organism=Homo sapiens, GI156713448, Length=424, Percent_Identity=28.3018867924528, Blast_Score=172, Evalue=7e-43,
Organism=Escherichia coli, GI48994898, Length=375, Percent_Identity=33.0666666666667, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1787970, Length=216, Percent_Identity=24.0740740740741, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI25143064, Length=376, Percent_Identity=32.7127659574468, Blast_Score=191, Evalue=7e-49,
Organism=Caenorhabditis elegans, GI17533177, Length=332, Percent_Identity=32.8313253012048, Blast_Score=113, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6319831, Length=375, Percent_Identity=32.5333333333333, Blast_Score=205, Evalue=8e-54,
Organism=Drosophila melanogaster, GI20129463, Length=387, Percent_Identity=32.8165374677003, Blast_Score=192, Evalue=2e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 43051; Mature: 43051

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYLDYAATTPASKEALDAFQKLSTDVYGNSSSLHDAGGKAENILRCCRQRMAELLSGEE
CEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
EGVYFTSGGTEANFLAIHSILNGLPAGKKRFLMTEMEHQSIHILKRHLEMLGFDVDLMKP
CCEEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECC
NASGIITTDILERHLRPDTGLVSIQHANSETGVIQPLAKLSAYLHKRDVLLHSDCVQTFA
CCCCEEEHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KIPVRIDELGADALSVSSHKVYGPKGVGAVYIRPGVNWKPVYPGTLHENGFKSGTVNVPG
HCCEEEHHCCCCCEECCCCEEECCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
IGAFITAADSTINRMDAAHLKFQELRAGFLEGVKDRKLPVSLAVPDSANSHVLPHIIGCF
CCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHHH
FHSFEGQYTMLECNRRGICISTGSACSAGYHEPSNTMKALLIPRELALQFVRISFGCDTT
HHHCCCCEEEEEECCCEEEEECCCCCCCCCCCCCCCHHEEECCHHHHHHHHHHEECCCCH
SEHVDRLLDVLEDMMNEKGDHRIDRRIKTYGS
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MIYLDYAATTPASKEALDAFQKLSTDVYGNSSSLHDAGGKAENILRCCRQRMAELLSGEE
CEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
EGVYFTSGGTEANFLAIHSILNGLPAGKKRFLMTEMEHQSIHILKRHLEMLGFDVDLMKP
CCEEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECC
NASGIITTDILERHLRPDTGLVSIQHANSETGVIQPLAKLSAYLHKRDVLLHSDCVQTFA
CCCCEEEHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KIPVRIDELGADALSVSSHKVYGPKGVGAVYIRPGVNWKPVYPGTLHENGFKSGTVNVPG
HCCEEEHHCCCCCEECCCCEEECCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
IGAFITAADSTINRMDAAHLKFQELRAGFLEGVKDRKLPVSLAVPDSANSHVLPHIIGCF
CCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHHH
FHSFEGQYTMLECNRRGICISTGSACSAGYHEPSNTMKALLIPRELALQFVRISFGCDTT
HHHCCCCEEEEEECCCEEEEECCCCCCCCCCCCCCCHHEEECCHHHHHHHHHHEECCCCH
SEHVDRLLDVLEDMMNEKGDHRIDRRIKTYGS
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8444804; 9384377 [H]