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The map label for this gene is aroE

Identifier: 52786494

GI number: 52786494

Start: 2652904

End: 2653740

Strand: Reverse

Name: aroE

Synonym: BLi02759

Alternate gene names: 52786494

Gene position: 2653740-2652904 (Counterclockwise)

Preceding gene: 52786495

Following gene: 52786493

Centisome position: 62.85

GC content: 50.06

Gene sequence:

>837_bases
ATGAAACCGATATACGGACTGATTGGCAATCCGGTGGCCCATTCCATGTCTCCGGACATCCATAATGCGGCTTTGAAGGA
TCTTTCTTTAGAAGGGCATTATCACGCTTTTCGCGTGGAAAACGAAGATCTTGAAGATGCTGTGAAAGGTATGCGGGCCC
TTGGCATTCAAGGGTTTAATGTGACCGTCCCCCACAAAGTATCGATCATGAAGCATCTCGACCGTATTGATGAAAGCGCC
GAAGCTCTCGGGGCGGTAAACACTGTCAGAAGAGAAAAAGAAGGGCTGGTCGGATATAATACAGATGGAGCAGGGTTTCT
TAAATCGCTGAAGCCATCCCTTGATCGTCCGCTTTCTGAGCTCTCGATACTGTTGATCGGAGCAGGCGGTGCGGCGAGGG
CGATATTTACCACTCTGGCAGCCGAGACGCCCAAGCGGCTTGATGTGGCGAATCGTACGCCTGAAAAGGCATTAGCATTC
ACTCAGCGTTTTGATGGAGAGGCACGCGCCTTGTCACTGCAGGAAGCGGAAGCAGACTTGTCAGCATATGATATCGTGAT
TCAAACGACGTCGGTCGGGATGCATCCGAATGTCGAAGCTGCCCCGCTTTCATTGGCGAATGCGAAAGAAACGTGCCTTG
TATGCGATATTATCTACAATCCGCTGAAAACGGCCCTGCTGCATGAGGCTGAAGCAAAAGGCTTGAAAACATTGGACGGA
GTCGGCATGTTCATCGGCCAGGCAGCCCTTGCTTTTGAGCTGTGGACAGGACATGAGCCGAATATGGAAAAAATGAAATC
AATTGTGTTGCAGCAACTAGGAGGAAAATCATGCTAA

Upstream 100 bases:

>100_bases
ACGGTCCAGGGGGCAAACCAAAAAATTGTCGCCCACGCCCCTAAAGGCGTTCACGTGTTTTTGCGCAGATCTCTAATATA
AGTGCTAAGGGGAGAGAGTT

Downstream 100 bases:

>100_bases
CAGGAAAACAAAAACGCTATTTGCGCCGGGAAGCGCATCATCTGTCACCGATTTTTCAAGTCGGCAAAGGCGGAGTCAAC
GAAAATATGGTCAAACAAAT

Product: shikimate 5-dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MKPIYGLIGNPVAHSMSPDIHNAALKDLSLEGHYHAFRVENEDLEDAVKGMRALGIQGFNVTVPHKVSIMKHLDRIDESA
EALGAVNTVRREKEGLVGYNTDGAGFLKSLKPSLDRPLSELSILLIGAGGAARAIFTTLAAETPKRLDVANRTPEKALAF
TQRFDGEARALSLQEAEADLSAYDIVIQTTSVGMHPNVEAAPLSLANAKETCLVCDIIYNPLKTALLHEAEAKGLKTLDG
VGMFIGQAALAFELWTGHEPNMEKMKSIVLQQLGGKSC

Sequences:

>Translated_278_residues
MKPIYGLIGNPVAHSMSPDIHNAALKDLSLEGHYHAFRVENEDLEDAVKGMRALGIQGFNVTVPHKVSIMKHLDRIDESA
EALGAVNTVRREKEGLVGYNTDGAGFLKSLKPSLDRPLSELSILLIGAGGAARAIFTTLAAETPKRLDVANRTPEKALAF
TQRFDGEARALSLQEAEADLSAYDIVIQTTSVGMHPNVEAAPLSLANAKETCLVCDIIYNPLKTALLHEAEAKGLKTLDG
VGMFIGQAALAFELWTGHEPNMEKMKSIVLQQLGGKSC
>Mature_278_residues
MKPIYGLIGNPVAHSMSPDIHNAALKDLSLEGHYHAFRVENEDLEDAVKGMRALGIQGFNVTVPHKVSIMKHLDRIDESA
EALGAVNTVRREKEGLVGYNTDGAGFLKSLKPSLDRPLSELSILLIGAGGAARAIFTTLAAETPKRLDVANRTPEKALAF
TQRFDGEARALSLQEAEADLSAYDIVIQTTSVGMHPNVEAAPLSLANAKETCLVCDIIYNPLKTALLHEAEAKGLKTLDG
VGMFIGQAALAFELWTGHEPNMEKMKSIVLQQLGGKSC

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; fourth step. [C]

COG id: COG0169

COG function: function code E; Shikimate 5-dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate dehydrogenase family

Homologues:

Organism=Escherichia coli, GI1787983, Length=277, Percent_Identity=35.7400722021661, Blast_Score=160, Evalue=9e-41,
Organism=Escherichia coli, GI1789675, Length=274, Percent_Identity=37.5912408759124, Blast_Score=141, Evalue=5e-35,
Organism=Saccharomyces cerevisiae, GI6320332, Length=267, Percent_Identity=26.2172284644195, Blast_Score=78, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROE_BACLD (Q65H34)

Other databases:

- EMBL:   CP000002
- EMBL:   AE017333
- RefSeq:   YP_079907.1
- RefSeq:   YP_092323.1
- HSSP:   Q58484
- ProteinModelPortal:   Q65H34
- SMR:   Q65H34
- STRING:   Q65H34
- EnsemblBacteria:   EBBACT00000056930
- EnsemblBacteria:   EBBACT00000060687
- GeneID:   3028801
- GeneID:   3097844
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi02759
- KEGG:   bli:BL02079
- NMPDR:   fig|279010.5.peg.3030
- eggNOG:   COG0169
- GeneTree:   EBGT00050000000826
- HOGENOM:   HBG553408
- OMA:   NTDGPGY
- ProtClustDB:   PRK00258
- BioCyc:   BLIC279010-1:BLI02759-MONOMER
- BioCyc:   BLIC279010:BL02079-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00222
- InterPro:   IPR016040
- InterPro:   IPR011342
- InterPro:   IPR013708
- InterPro:   IPR022893
- InterPro:   IPR006151
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR00507

Pfam domain/function: PF01488 Shikimate_DH; PF08501 Shikimate_dh_N

EC number: =1.1.1.25

Molecular weight: Translated: 29910; Mature: 29910

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: ACT_SITE 66-66

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPIYGLIGNPVAHSMSPDIHNAALKDLSLEGHYHAFRVENEDLEDAVKGMRALGIQGFN
CCCCCCCCCCCHHHCCCCCHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCE
VTVPHKVSIMKHLDRIDESAEALGAVNTVRREKEGLVGYNTDGAGFLKSLKPSLDRPLSE
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHCHHHCCCHHH
LSILLIGAGGAARAIFTTLAAETPKRLDVANRTPEKALAFTQRFDGEARALSLQEAEADL
EEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCHHEEEHHHHHCCC
SAYDIVIQTTSVGMHPNVEAAPLSLANAKETCLVCDIIYNPLKTALLHEAEAKGLKTLDG
CCEEEEEEEECCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCHHHHH
VGMFIGQAALAFELWTGHEPNMEKMKSIVLQQLGGKSC
HHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKPIYGLIGNPVAHSMSPDIHNAALKDLSLEGHYHAFRVENEDLEDAVKGMRALGIQGFN
CCCCCCCCCCCHHHCCCCCHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCE
VTVPHKVSIMKHLDRIDESAEALGAVNTVRREKEGLVGYNTDGAGFLKSLKPSLDRPLSE
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHCHHHCCCHHH
LSILLIGAGGAARAIFTTLAAETPKRLDVANRTPEKALAFTQRFDGEARALSLQEAEADL
EEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCHHEEEHHHHHCCC
SAYDIVIQTTSVGMHPNVEAAPLSLANAKETCLVCDIIYNPLKTALLHEAEAKGLKTLDG
CCEEEEEEEECCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCHHHHH
VGMFIGQAALAFELWTGHEPNMEKMKSIVLQQLGGKSC
HHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA