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The map label for this gene is glyS [H]

Identifier: 52786452

GI number: 52786452

Start: 2612910

End: 2614949

Strand: Reverse

Name: glyS [H]

Synonym: BLi02717

Alternate gene names: 52786452

Gene position: 2614949-2612910 (Counterclockwise)

Preceding gene: 52786453

Following gene: 52786451

Centisome position: 61.93

GC content: 49.12

Gene sequence:

>2040_bases
ATGAGTAATCAAGATTTGCTTTTAGAAATCGGATTAGAAGAAATGCCGGCGCGCTTCATGCATGACAGCATGACTCAGCT
GGGTGAGAAGCTGGAAAGCTGGCTCAAGGAAAAAAATATTGCCTTCGGTTCCGTCAAGCTGTATAACTCCCCTAGAAGAC
TGGCCGTTCTTGTCGAGAACGTGGCGGAGAAGCAGGATGATGTGAAAGAAGAAGCGAAAGGACCGGCAAAGAAAATCGCC
CTTGACGAAAACGGCGAATGGACAAAGGCGGCCATCGGCTTTTCACGGGGGCAAGGAGCAAGTCCGGAAGATTTGTATTT
TAAAGACATCAAAGGAACGGAATATGTATTTGTGCAAAAATTCCAAAAAGGCCAGGATACGTTTTCATTGCTTCCTGAGC
TCCGCGATTTGGTGACAAGCCTCCACTTTCCGAAAAACATGCGCTGGGGCAGCGAAGATTTAAAATATATCAGGCCGATT
AAATGGATCGTCGCGCTGTTCGGAAGTGAAGTCGTTCCGTTTTCAGTCGCTCATGTCGAAACCGGACGCACAACGCAGGG
ACACCGCTTCCTCGGAAAAGCAGCCGACTTGAGCGCACCTTCTGAATACGAAGCGACACTGGAAAGCCAGTTTGTGATTG
CCGATCCGGCCAAACGGAAGCAGCTGATCGTTAAGCAGCTTGACGAACTGGCTGCTGAGAAGTCCTGGGACATTCCGAGA
GACGAGGATCTGCTGGATGAGGTCAACCATCTGGTTGAATATCCAACGGTACTGTTCGGTTCTTACGAAGAGGAATTTCT
CGCAATTCCTGAAGAGGTGCTCGTGACAACAATGAAAGAGCATCAGCGTTATTTCCCTGTCAAAGATGAAAACGGCAGCT
TGCTTCCTTACTTTGTGACCGTCCGCAACGGAGACAGCCGCGCCCTTGAAAACGTGGCGAGAGGGAATGAAAAAGTGCTG
AGAGCAAGGCTTTCAGACGCTTCCTTCTTCTATAAAGAAGATCAAAAATTAAACATTGACGAGAATGTGAGAAAGCTCGA
AAAGATCGTTTTCCATGAAGAACTGGGGTCGCTCGGAGATAAAGTGAGACGCACTGAGGCGCTTGCTGAACGGATCGCTG
AAGTCATCGGTGCCGATCAAGAGACGGTGCAGCTGATCAAGCGCGCTGCGCACATTTCAAAATTCGACCTTGTGACACAC
ATGGTTTACGAATTTCCTGAGCTGCAGGGCATCATGGGTGAAAAATATGCGCGGATGCTCGGTGAGAAAGAAGAAGTTGC
GCTCGCAGTCAATGAGCACTATATGCCGAGACAAGCCGGAGGACAAACGCCTTCATCAATCGCCGGCGCCGTCGTCGCAT
TGGCGGACAAACTCGATACGGTCGCTTCATTCTTCAAAATCGGCGTCATTCCGACAGGATCTCAGGATCCGTATGGACTG
AGAAGACAGGCGAGCGGCATCGTCCAAATCCTGCTTGACCGGAACTGGGGCATTTCATTCAAAGAACTGGCTGCGTTTGC
CGGACAGGAAGCAAACAGCGAACTGCTCGAATTCTTTAAACAGCGCCTCAAATATGTATTAACAGCGGAAAACATCCGAT
ACGACGTCGTGGATGCCGTGCTCGAAAGCACGAACCTTGAGCCGTATTCGGCTGTCAAAAAAGCCGCCGTCCTTGAATCG
GCTGCAGCCAAACCGGAATTTAAAGAAACGGCTGAAGCGCTGGCAAGGGTTATTTCGATCAGCAAAAAGAGCGAAACGAG
CGCCATTGACGCTTCACTGTTTGAAAACCCTCAAGAAGAGGAGCTTTTCAAAGCTTATGAGGCGTGCAGGCAGCAAATAG
AGACCCTTTACCAAGCAAAAGATTATGAGGGCGCCTTCAGTGAGCTGTCCAAGCTGAAAGAGCCGATTGAAGCGTATTTT
GATCATACGATGGTACATGCCGAAGATGAAAGATTAAAAGCAAACCGCCTGGCGCAAATGGCAAGCTTGGCGGAGCTGAT
CCGATCTTTTGCAAACATCAATGCCATCATTGTAAAATAA

Upstream 100 bases:

>100_bases
ATCGCAAGGGTGAGAAACCTGGCCAGAAAAGTGGCGAAAACGTATTATGATGAACGCGAAAAGTTGGGTTTCCCAATGCT
GAAGGAGGAAGAGGCTTCAC

Downstream 100 bases:

>100_bases
ACACCGCAGAAGACGGTTGCTGACAGGCTGGCAATCGTCTTTTTGCTCAAATCGATTTTTCACATCCTTGTCAAATACCG
CCTGTCTTTTTCATAGAATT

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 679; Mature: 678

Protein sequence:

>679_residues
MSNQDLLLEIGLEEMPARFMHDSMTQLGEKLESWLKEKNIAFGSVKLYNSPRRLAVLVENVAEKQDDVKEEAKGPAKKIA
LDENGEWTKAAIGFSRGQGASPEDLYFKDIKGTEYVFVQKFQKGQDTFSLLPELRDLVTSLHFPKNMRWGSEDLKYIRPI
KWIVALFGSEVVPFSVAHVETGRTTQGHRFLGKAADLSAPSEYEATLESQFVIADPAKRKQLIVKQLDELAAEKSWDIPR
DEDLLDEVNHLVEYPTVLFGSYEEEFLAIPEEVLVTTMKEHQRYFPVKDENGSLLPYFVTVRNGDSRALENVARGNEKVL
RARLSDASFFYKEDQKLNIDENVRKLEKIVFHEELGSLGDKVRRTEALAERIAEVIGADQETVQLIKRAAHISKFDLVTH
MVYEFPELQGIMGEKYARMLGEKEEVALAVNEHYMPRQAGGQTPSSIAGAVVALADKLDTVASFFKIGVIPTGSQDPYGL
RRQASGIVQILLDRNWGISFKELAAFAGQEANSELLEFFKQRLKYVLTAENIRYDVVDAVLESTNLEPYSAVKKAAVLES
AAAKPEFKETAEALARVISISKKSETSAIDASLFENPQEEELFKAYEACRQQIETLYQAKDYEGAFSELSKLKEPIEAYF
DHTMVHAEDERLKANRLAQMASLAELIRSFANINAIIVK

Sequences:

>Translated_679_residues
MSNQDLLLEIGLEEMPARFMHDSMTQLGEKLESWLKEKNIAFGSVKLYNSPRRLAVLVENVAEKQDDVKEEAKGPAKKIA
LDENGEWTKAAIGFSRGQGASPEDLYFKDIKGTEYVFVQKFQKGQDTFSLLPELRDLVTSLHFPKNMRWGSEDLKYIRPI
KWIVALFGSEVVPFSVAHVETGRTTQGHRFLGKAADLSAPSEYEATLESQFVIADPAKRKQLIVKQLDELAAEKSWDIPR
DEDLLDEVNHLVEYPTVLFGSYEEEFLAIPEEVLVTTMKEHQRYFPVKDENGSLLPYFVTVRNGDSRALENVARGNEKVL
RARLSDASFFYKEDQKLNIDENVRKLEKIVFHEELGSLGDKVRRTEALAERIAEVIGADQETVQLIKRAAHISKFDLVTH
MVYEFPELQGIMGEKYARMLGEKEEVALAVNEHYMPRQAGGQTPSSIAGAVVALADKLDTVASFFKIGVIPTGSQDPYGL
RRQASGIVQILLDRNWGISFKELAAFAGQEANSELLEFFKQRLKYVLTAENIRYDVVDAVLESTNLEPYSAVKKAAVLES
AAAKPEFKETAEALARVISISKKSETSAIDASLFENPQEEELFKAYEACRQQIETLYQAKDYEGAFSELSKLKEPIEAYF
DHTMVHAEDERLKANRLAQMASLAELIRSFANINAIIVK
>Mature_678_residues
SNQDLLLEIGLEEMPARFMHDSMTQLGEKLESWLKEKNIAFGSVKLYNSPRRLAVLVENVAEKQDDVKEEAKGPAKKIAL
DENGEWTKAAIGFSRGQGASPEDLYFKDIKGTEYVFVQKFQKGQDTFSLLPELRDLVTSLHFPKNMRWGSEDLKYIRPIK
WIVALFGSEVVPFSVAHVETGRTTQGHRFLGKAADLSAPSEYEATLESQFVIADPAKRKQLIVKQLDELAAEKSWDIPRD
EDLLDEVNHLVEYPTVLFGSYEEEFLAIPEEVLVTTMKEHQRYFPVKDENGSLLPYFVTVRNGDSRALENVARGNEKVLR
ARLSDASFFYKEDQKLNIDENVRKLEKIVFHEELGSLGDKVRRTEALAERIAEVIGADQETVQLIKRAAHISKFDLVTHM
VYEFPELQGIMGEKYARMLGEKEEVALAVNEHYMPRQAGGQTPSSIAGAVVALADKLDTVASFFKIGVIPTGSQDPYGLR
RQASGIVQILLDRNWGISFKELAAFAGQEANSELLEFFKQRLKYVLTAENIRYDVVDAVLESTNLEPYSAVKKAAVLESA
AAKPEFKETAEALARVISISKKSETSAIDASLFENPQEEELFKAYEACRQQIETLYQAKDYEGAFSELSKLKEPIEAYFD
HTMVHAEDERLKANRLAQMASLAELIRSFANINAIIVK

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=693, Percent_Identity=35.3535353535354, Blast_Score=419, Evalue=1e-118,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 76544; Mature: 76413

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNQDLLLEIGLEEMPARFMHDSMTQLGEKLESWLKEKNIAFGSVKLYNSPRRLAVLVEN
CCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHH
VAEKQDDVKEEAKGPAKKIALDENGEWTKAAIGFSRGQGASPEDLYFKDIKGTEYVFVQK
HHHHHHHHHHHHCCCHHEEEECCCCCEEHHHHCCCCCCCCCCCCCEEECCCCCHHHHHHH
FQKGQDTFSLLPELRDLVTSLHFPKNMRWGSEDLKYIRPIKWIVALFGSEVVPFSVAHVE
HHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEC
TGRTTQGHRFLGKAADLSAPSEYEATLESQFVIADPAKRKQLIVKQLDELAAEKSWDIPR
CCCCCCCHHHHCCHHCCCCCCHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHCCCCCCC
DEDLLDEVNHLVEYPTVLFGSYEEEFLAIPEEVLVTTMKEHQRYFPVKDENGSLLPYFVT
CHHHHHHHHHHHHCCHHHHCCCCHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEE
VRNGDSRALENVARGNEKVLRARLSDASFFYKEDQKLNIDENVRKLEKIVFHEELGSLGD
EECCCHHHHHHHHCCHHHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
KVRRTEALAERIAEVIGADQETVQLIKRAAHISKFDLVTHMVYEFPELQGIMGEKYARML
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GEKEEVALAVNEHYMPRQAGGQTPSSIAGAVVALADKLDTVASFFKIGVIPTGSQDPYGL
CCCHHEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCH
RRQASGIVQILLDRNWGISFKELAAFAGQEANSELLEFFKQRLKYVLTAENIRYDVVDAV
HHHHHHHHHHEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHH
LESTNLEPYSAVKKAAVLESAAAKPEFKETAEALARVISISKKSETSAIDASLFENPQEE
HHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHH
ELFKAYEACRQQIETLYQAKDYEGAFSELSKLKEPIEAYFDHTMVHAEDERLKANRLAQM
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHH
ASLAELIRSFANINAIIVK
HHHHHHHHHHHCCCEEEEC
>Mature Secondary Structure 
SNQDLLLEIGLEEMPARFMHDSMTQLGEKLESWLKEKNIAFGSVKLYNSPRRLAVLVEN
CCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHH
VAEKQDDVKEEAKGPAKKIALDENGEWTKAAIGFSRGQGASPEDLYFKDIKGTEYVFVQK
HHHHHHHHHHHHCCCHHEEEECCCCCEEHHHHCCCCCCCCCCCCCEEECCCCCHHHHHHH
FQKGQDTFSLLPELRDLVTSLHFPKNMRWGSEDLKYIRPIKWIVALFGSEVVPFSVAHVE
HHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEC
TGRTTQGHRFLGKAADLSAPSEYEATLESQFVIADPAKRKQLIVKQLDELAAEKSWDIPR
CCCCCCCHHHHCCHHCCCCCCHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHCCCCCCC
DEDLLDEVNHLVEYPTVLFGSYEEEFLAIPEEVLVTTMKEHQRYFPVKDENGSLLPYFVT
CHHHHHHHHHHHHCCHHHHCCCCHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEE
VRNGDSRALENVARGNEKVLRARLSDASFFYKEDQKLNIDENVRKLEKIVFHEELGSLGD
EECCCHHHHHHHHCCHHHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
KVRRTEALAERIAEVIGADQETVQLIKRAAHISKFDLVTHMVYEFPELQGIMGEKYARML
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GEKEEVALAVNEHYMPRQAGGQTPSSIAGAVVALADKLDTVASFFKIGVIPTGSQDPYGL
CCCHHEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCH
RRQASGIVQILLDRNWGISFKELAAFAGQEANSELLEFFKQRLKYVLTAENIRYDVVDAV
HHHHHHHHHHEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHH
LESTNLEPYSAVKKAAVLESAAAKPEFKETAEALARVISISKKSETSAIDASLFENPQEE
HHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHH
ELFKAYEACRQQIETLYQAKDYEGAFSELSKLKEPIEAYFDHTMVHAEDERLKANRLAQM
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHH
ASLAELIRSFANINAIIVK
HHHHHHHHHHHCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]