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The map label for this gene is dnaG [H]

Identifier: 52786448

GI number: 52786448

Start: 2608624

End: 2610447

Strand: Reverse

Name: dnaG [H]

Synonym: BLi02713

Alternate gene names: 52786448

Gene position: 2610447-2608624 (Counterclockwise)

Preceding gene: 52786449

Following gene: 52786447

Centisome position: 61.82

GC content: 46.82

Gene sequence:

>1824_bases
ATGGGCAATCGTATACCAGACGAAATTGTAGAACAGGTGCAGAAATCAGCTGATATCGTAGAAGTTATCGGAGAGTACGT
CCAGTTAAGAAAACAGGGGCGTAATTATTTTGGGCTTTGTCCTTTTCATGGTGAGAACACACCATCTTTTTCTGTTTCTG
GAGAAAAACAGATTTTTCATTGTTTTGGCTGCGGAGCGGGAGGGAACGTTTTTTCATTTTTAAGACAAATGGAAGGCTAT
TCATTTACCGAGGCTGTCTCTCACCTGGCGGACAAATATCAAATAGATCTGCCGGAGCAGGTCGCAGCTGTATCAGCATA
CAGCACAGGCAGTTCGGATGAACAGAAAATGGCGGAAGCTCATGATCTGTTAAAGAAATTTTATCATCATTTGCTCGTCA
ATACAAAGGAAGGCCAGGCAGCGCTCGATTATTTGCTTGAAAGAGGCTTTAAGATGGAGCAGATCGAGCAGTTTGAAATC
GGATGCGCGCTTGACTCGTGGGATTTCATCACGAAGTTTTTGGAACGGAGAGGTTTTGATCTTGCGCTGATGGAAAGAGC
TGGACTCATCATCAGACGCGAGAACGGCGACGGATATTTTGACCGTTTCAGAAACAGGATTATGTTTCCGATTCATGATC
ATCACGGCACAACGGTCGCTTTTTCCGGAAGGACGTTCACCGGGGAGCAGCCAAAATACATGAACAGCCCGGAAACGCCG
CTTTTTCGAAAAAGCAAGCTTCTCTATCATTTCCATCAGGCCCGCCTTCACATTCGGAAAGAAGAAAGGGCCGTCCTTTT
TGAAGGATTTGCCGATGTCATTTCAGCCGTCACCTCCGGTGTGAAAGAATCGATCGCCACGATGGGTACATCGCTGACGG
AAGAACATGCGAAGATCATCAGGCGAAATGTCGGAGAGGTCATACTGTGCTATGACTCGGATCATGCCGGTTATGAAGCC
ACGCTGAAAGCGGCGGAGATTCTTCAGAAAAAAGGCTGCAACGTCAAGGTGGCCATGATACCTGACGGGCTGGATCCCGA
TGACTACATCAAAAAATTCGGCAGCGAGAAATTCAAAAATGATGTAATAGGGGCCAGCGTTACGATGATGACGTTTAAAA
TGAACTATTTCCGCAAGGGCAGAAACCTGTCTGATGAAGGAGACCGTTTAGCCTATATCAACGATGTACTGAAGGAAGTC
AGCCTTCTCAGCGGTCCTTTGGAACAGGAAATATACATCAAACAGCTGGCAAATGAATTTTCAATCTCATTTGAATCCTT
GAAGGATCAGCTTTCCGTATTTGAAAAGCAGGCTGCAAGCCGCAGCAGGGAAAGCAGCCGCCAGGACTCTAAAGAGCGGC
AGGCGGCCGTATCGCGCGCCCCTCGGAGAAAGACGGGGCTTCGGCCGGCTCATGAGAATGCGGAGCGGATGCTCCTGGCC
CACATGATTCATGACCGGGACGTCATTCGAAAGGTTTTGGAACGGGTGGGCTTTGAATTCAACCTTGATCAGCACCGAGC
GATTGCCGCTTATTTGTACGCCATGTATGAAGAAGGAGCGGACATTTCCATCCAGGAGCTCTTTAAGAGAGTCGAGGATC
AGGAACTTGGCCAGCTTCTGACAGATATCTTAATGCTTCAGGTGAACGAAGAATTAAGTGAAGCGGAATTATCTGATTAT
GTAAAAAAAGTGTTGAATTATCGAAATTGGTCAATGATAAAAGAAAAAGAAGCTGCAAGGGCGGAAGCCGAGAGGCAAAA
AGACTTTATCAAAGCGGCGACTTTGGCGCAGGAAATTGTTAAGTTGAACCGTTCGCTGAAATAA

Upstream 100 bases:

>100_bases
AAGACCGGGAGCGATTTGTCAAAGAGCTGCAAAAAATCTTGTCGAAAATTGAAGGATTTACGTACTAAGCAGCGAATTAT
GTACGACGGAGTGTTGGAAC

Downstream 100 bases:

>100_bases
ACCGTTATATATAATAGGAAAAGCTCTTGGCAATGAATCCTAACGGAGGAGCAAAAGCTGCTTGTCCTAATCAATATATT
GTCAATTTATGATTTGCTTT

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 607; Mature: 606

Protein sequence:

>607_residues
MGNRIPDEIVEQVQKSADIVEVIGEYVQLRKQGRNYFGLCPFHGENTPSFSVSGEKQIFHCFGCGAGGNVFSFLRQMEGY
SFTEAVSHLADKYQIDLPEQVAAVSAYSTGSSDEQKMAEAHDLLKKFYHHLLVNTKEGQAALDYLLERGFKMEQIEQFEI
GCALDSWDFITKFLERRGFDLALMERAGLIIRRENGDGYFDRFRNRIMFPIHDHHGTTVAFSGRTFTGEQPKYMNSPETP
LFRKSKLLYHFHQARLHIRKEERAVLFEGFADVISAVTSGVKESIATMGTSLTEEHAKIIRRNVGEVILCYDSDHAGYEA
TLKAAEILQKKGCNVKVAMIPDGLDPDDYIKKFGSEKFKNDVIGASVTMMTFKMNYFRKGRNLSDEGDRLAYINDVLKEV
SLLSGPLEQEIYIKQLANEFSISFESLKDQLSVFEKQAASRSRESSRQDSKERQAAVSRAPRRKTGLRPAHENAERMLLA
HMIHDRDVIRKVLERVGFEFNLDQHRAIAAYLYAMYEEGADISIQELFKRVEDQELGQLLTDILMLQVNEELSEAELSDY
VKKVLNYRNWSMIKEKEAARAEAERQKDFIKAATLAQEIVKLNRSLK

Sequences:

>Translated_607_residues
MGNRIPDEIVEQVQKSADIVEVIGEYVQLRKQGRNYFGLCPFHGENTPSFSVSGEKQIFHCFGCGAGGNVFSFLRQMEGY
SFTEAVSHLADKYQIDLPEQVAAVSAYSTGSSDEQKMAEAHDLLKKFYHHLLVNTKEGQAALDYLLERGFKMEQIEQFEI
GCALDSWDFITKFLERRGFDLALMERAGLIIRRENGDGYFDRFRNRIMFPIHDHHGTTVAFSGRTFTGEQPKYMNSPETP
LFRKSKLLYHFHQARLHIRKEERAVLFEGFADVISAVTSGVKESIATMGTSLTEEHAKIIRRNVGEVILCYDSDHAGYEA
TLKAAEILQKKGCNVKVAMIPDGLDPDDYIKKFGSEKFKNDVIGASVTMMTFKMNYFRKGRNLSDEGDRLAYINDVLKEV
SLLSGPLEQEIYIKQLANEFSISFESLKDQLSVFEKQAASRSRESSRQDSKERQAAVSRAPRRKTGLRPAHENAERMLLA
HMIHDRDVIRKVLERVGFEFNLDQHRAIAAYLYAMYEEGADISIQELFKRVEDQELGQLLTDILMLQVNEELSEAELSDY
VKKVLNYRNWSMIKEKEAARAEAERQKDFIKAATLAQEIVKLNRSLK
>Mature_606_residues
GNRIPDEIVEQVQKSADIVEVIGEYVQLRKQGRNYFGLCPFHGENTPSFSVSGEKQIFHCFGCGAGGNVFSFLRQMEGYS
FTEAVSHLADKYQIDLPEQVAAVSAYSTGSSDEQKMAEAHDLLKKFYHHLLVNTKEGQAALDYLLERGFKMEQIEQFEIG
CALDSWDFITKFLERRGFDLALMERAGLIIRRENGDGYFDRFRNRIMFPIHDHHGTTVAFSGRTFTGEQPKYMNSPETPL
FRKSKLLYHFHQARLHIRKEERAVLFEGFADVISAVTSGVKESIATMGTSLTEEHAKIIRRNVGEVILCYDSDHAGYEAT
LKAAEILQKKGCNVKVAMIPDGLDPDDYIKKFGSEKFKNDVIGASVTMMTFKMNYFRKGRNLSDEGDRLAYINDVLKEVS
LLSGPLEQEIYIKQLANEFSISFESLKDQLSVFEKQAASRSRESSRQDSKERQAAVSRAPRRKTGLRPAHENAERMLLAH
MIHDRDVIRKVLERVGFEFNLDQHRAIAAYLYAMYEEGADISIQELFKRVEDQELGQLLTDILMLQVNEELSEAELSDYV
KKVLNYRNWSMIKEKEAARAEAERQKDFIKAATLAQEIVKLNRSLK

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=538, Percent_Identity=31.7843866171004, Blast_Score=259, Evalue=2e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007693
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 69357; Mature: 69226

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNRIPDEIVEQVQKSADIVEVIGEYVQLRKQGRNYFGLCPFHGENTPSFSVSGEKQIFH
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCEEE
CFGCGAGGNVFSFLRQMEGYSFTEAVSHLADKYQIDLPEQVAAVSAYSTGSSDEQKMAEA
EECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHH
HDLLKKFYHHLLVNTKEGQAALDYLLERGFKMEQIEQFEIGCALDSWDFITKFLERRGFD
HHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCC
LALMERAGLIIRRENGDGYFDRFRNRIMFPIHDHHGTTVAFSGRTFTGEQPKYMNSPETP
EEEHHHCCEEEEECCCCCHHHHHCCCEEEEEECCCCCEEEECCCEECCCCCCCCCCCCCC
LFRKSKLLYHFHQARLHIRKEERAVLFEGFADVISAVTSGVKESIATMGTSLTEEHAKII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
RRNVGEVILCYDSDHAGYEATLKAAEILQKKGCNVKVAMIPDGLDPDDYIKKFGSEKFKN
HHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCHHHHH
DVIGASVTMMTFKMNYFRKGRNLSDEGDRLAYINDVLKEVSLLSGPLEQEIYIKQLANEF
CCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
SISFESLKDQLSVFEKQAASRSRESSRQDSKERQAAVSRAPRRKTGLRPAHENAERMLLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHH
HMIHDRDVIRKVLERVGFEFNLDQHRAIAAYLYAMYEEGADISIQELFKRVEDQELGQLL
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCHHHHHHHH
TDILMLQVNEELSEAELSDYVKKVLNYRNWSMIKEKEAARAEAERQKDFIKAATLAQEIV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLNRSLK
HHHHCCC
>Mature Secondary Structure 
GNRIPDEIVEQVQKSADIVEVIGEYVQLRKQGRNYFGLCPFHGENTPSFSVSGEKQIFH
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCEEE
CFGCGAGGNVFSFLRQMEGYSFTEAVSHLADKYQIDLPEQVAAVSAYSTGSSDEQKMAEA
EECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHH
HDLLKKFYHHLLVNTKEGQAALDYLLERGFKMEQIEQFEIGCALDSWDFITKFLERRGFD
HHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCC
LALMERAGLIIRRENGDGYFDRFRNRIMFPIHDHHGTTVAFSGRTFTGEQPKYMNSPETP
EEEHHHCCEEEEECCCCCHHHHHCCCEEEEEECCCCCEEEECCCEECCCCCCCCCCCCCC
LFRKSKLLYHFHQARLHIRKEERAVLFEGFADVISAVTSGVKESIATMGTSLTEEHAKII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
RRNVGEVILCYDSDHAGYEATLKAAEILQKKGCNVKVAMIPDGLDPDDYIKKFGSEKFKN
HHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCHHHHH
DVIGASVTMMTFKMNYFRKGRNLSDEGDRLAYINDVLKEVSLLSGPLEQEIYIKQLANEF
CCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
SISFESLKDQLSVFEKQAASRSRESSRQDSKERQAAVSRAPRRKTGLRPAHENAERMLLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHH
HMIHDRDVIRKVLERVGFEFNLDQHRAIAAYLYAMYEEGADISIQELFKRVEDQELGQLL
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCHHHHHHHH
TDILMLQVNEELSEAELSDYVKKVLNYRNWSMIKEKEAARAEAERQKDFIKAATLAQEIV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLNRSLK
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3919021; 3086839; 8969508; 9384377 [H]