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The map label for this gene is thyA

Identifier: 52785784

GI number: 52785784

Start: 1974955

End: 1975794

Strand: Direct

Name: thyA

Synonym: BLi02029

Alternate gene names: 52785784

Gene position: 1974955-1975794 (Clockwise)

Preceding gene: 52785783

Following gene: 52785785

Centisome position: 46.77

GC content: 44.17

Gene sequence:

>840_bases
ATGAGCCATTATGACCAGCAATACAATGCAATCATACAAAAAATTATTGAATCAGGGATCTCCGATGAAGAATATCAGGT
TCGGACGAAGTGGGATTCAGACGGAACGCCGGCTCATACGCTGAGCATCATGAGCGAAAAAATGCGGTTTGACAATTCAG
AAGTGCCGATTTTGACGACGAAGAAGGTGGCGTGGAAAACGGCCATTAAAGAGCTGCTTTGGATTTGGCAGCTGAAATCC
AATGATGTTCAAGTTCTGAATGATATGGGGGTTCATATTTGGGATCAATGGCGTCTTGAAGACGGCACAATCGGAGCAGC
GTACGGCTATCAATTAGGGAAGAAAAACAGAACGGTTAACGGCCAGAAAGTCGATCAAGTGGATTATTTGCTTCATCAGT
TAAAGCATAACCCTTCTTCACGCAGGCATTTGACCATGCTGTGGAATCCCGATGATTTGGATGGCATGGCTTTGACACCC
TGCGTCTATGAGACGCAATGGTATGTGAAAGAAGGAAAGCTCTCTTTAGAAGTAAGAGCGAGAAGCAACGACATGGCGCT
TGGCAACCCGTTCAATGTGTTTCAATATAATGTCTTGCAGCGCATGATCGCGCAAGTCCTCGGTTATGAGCTGGGCGAAT
ACATCTTCAACATCGGTGACTGCCACATCTATACCCGTCACATCGACAATTTAAACATTCAGATGAAACGCGAGCAGTAT
GAAGCGCCAAAGCTATGGATCAACCCGGATATCAAAAATTTTTATGACTTTACAATCGATGATTTTAAATTAATCGACTA
TAAGCACGGCGACAAGCTGACATTTGAAGTTGCCGTATGA

Upstream 100 bases:

>100_bases
TTCTCAAGAAAGCATTGGAGATTTATCAAACCCGCGCGTGATCTTAAACCTTGAAAAAAACTCATTTTATACATATCATG
TGGAAAGAGGAGAGGATAAG

Downstream 100 bases:

>100_bases
ACAGGCGGATTTCCCGCCTGTTTTTTTGTACAGTGATGAAAAACGGCCTTGGAGGATGATTCAAGAAGATACAGACATAT
AATACGGCAAGACAGGCACA

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MSHYDQQYNAIIQKIIESGISDEEYQVRTKWDSDGTPAHTLSIMSEKMRFDNSEVPILTTKKVAWKTAIKELLWIWQLKS
NDVQVLNDMGVHIWDQWRLEDGTIGAAYGYQLGKKNRTVNGQKVDQVDYLLHQLKHNPSSRRHLTMLWNPDDLDGMALTP
CVYETQWYVKEGKLSLEVRARSNDMALGNPFNVFQYNVLQRMIAQVLGYELGEYIFNIGDCHIYTRHIDNLNIQMKREQY
EAPKLWINPDIKNFYDFTIDDFKLIDYKHGDKLTFEVAV

Sequences:

>Translated_279_residues
MSHYDQQYNAIIQKIIESGISDEEYQVRTKWDSDGTPAHTLSIMSEKMRFDNSEVPILTTKKVAWKTAIKELLWIWQLKS
NDVQVLNDMGVHIWDQWRLEDGTIGAAYGYQLGKKNRTVNGQKVDQVDYLLHQLKHNPSSRRHLTMLWNPDDLDGMALTP
CVYETQWYVKEGKLSLEVRARSNDMALGNPFNVFQYNVLQRMIAQVLGYELGEYIFNIGDCHIYTRHIDNLNIQMKREQY
EAPKLWINPDIKNFYDFTIDDFKLIDYKHGDKLTFEVAV
>Mature_278_residues
SHYDQQYNAIIQKIIESGISDEEYQVRTKWDSDGTPAHTLSIMSEKMRFDNSEVPILTTKKVAWKTAIKELLWIWQLKSN
DVQVLNDMGVHIWDQWRLEDGTIGAAYGYQLGKKNRTVNGQKVDQVDYLLHQLKHNPSSRRHLTMLWNPDDLDGMALTPC
VYETQWYVKEGKLSLEVRARSNDMALGNPFNVFQYNVLQRMIAQVLGYELGEYIFNIGDCHIYTRHIDNLNIQMKREQYE
APKLWINPDIKNFYDFTIDDFKLIDYKHGDKLTFEVAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily

Homologues:

Organism=Homo sapiens, GI4507751, Length=298, Percent_Identity=35.9060402684564, Blast_Score=177, Evalue=8e-45,
Organism=Escherichia coli, GI1789191, Length=275, Percent_Identity=36, Blast_Score=179, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI71993377, Length=267, Percent_Identity=37.8277153558052, Blast_Score=180, Evalue=7e-46,
Organism=Saccharomyces cerevisiae, GI6324648, Length=228, Percent_Identity=38.5964912280702, Blast_Score=149, Evalue=4e-37,
Organism=Drosophila melanogaster, GI17137556, Length=268, Percent_Identity=35.4477611940299, Blast_Score=172, Evalue=3e-43,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): TYSY_BACLD (Q65J44)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_079196.1
- RefSeq:   YP_091613.1
- ProteinModelPortal:   Q65J44
- SMR:   Q65J44
- STRING:   Q65J44
- EnsemblBacteria:   EBBACT00000057358
- EnsemblBacteria:   EBBACT00000061151
- GeneID:   3031282
- GeneID:   3099538
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi02029
- KEGG:   bli:BL02642
- NMPDR:   fig|279010.5.peg.2728
- eggNOG:   COG0207
- GeneTree:   EBGT00050000001560
- HOGENOM:   HBG588098
- OMA:   KSWDANA
- ProtClustDB:   PRK01827
- BioCyc:   BLIC279010-1:BLI02029-MONOMER
- BioCyc:   BLIC279010:BL02642-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00008
- InterPro:   IPR000398
- InterPro:   IPR020940
- Gene3D:   G3DSA:3.30.572.10
- PANTHER:   PTHR11549:SF2
- PRINTS:   PR00108
- TIGRFAMs:   TIGR03284

Pfam domain/function: PF00303 Thymidylat_synt; SSF55831 Thymidylat_synth_C

EC number: =2.1.1.45

Molecular weight: Translated: 32761; Mature: 32629

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: ACT_SITE 161-161

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHYDQQYNAIIQKIIESGISDEEYQVRTKWDSDGTPAHTLSIMSEKMRFDNSEVPILTT
CCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEE
KKVAWKTAIKELLWIWQLKSNDVQVLNDMGVHIWDQWRLEDGTIGAAYGYQLGKKNRTVN
CHHHHHHHHHHHHHEEEECCCCEEEEECCCCEEEEEEEECCCCEEHHHHHEECCCCCCCC
GQKVDQVDYLLHQLKHNPSSRRHLTMLWNPDDLDGMALTPCVYETQWYVKEGKLSLEVRA
CCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCEEECCEEECCEEEEECCCEEEEEEE
RSNDMALGNPFNVFQYNVLQRMIAQVLGYELGEYIFNIGDCHIYTRHIDNLNIQMKREQY
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCCC
EAPKLWINPDIKNFYDFTIDDFKLIDYKHGDKLTFEVAV
CCCEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEEEEC
>Mature Secondary Structure 
SHYDQQYNAIIQKIIESGISDEEYQVRTKWDSDGTPAHTLSIMSEKMRFDNSEVPILTT
CCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEE
KKVAWKTAIKELLWIWQLKSNDVQVLNDMGVHIWDQWRLEDGTIGAAYGYQLGKKNRTVN
CHHHHHHHHHHHHHEEEECCCCEEEEECCCCEEEEEEEECCCCEEHHHHHEECCCCCCCC
GQKVDQVDYLLHQLKHNPSSRRHLTMLWNPDDLDGMALTPCVYETQWYVKEGKLSLEVRA
CCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCEEECCEEECCEEEEECCCEEEEEEE
RSNDMALGNPFNVFQYNVLQRMIAQVLGYELGEYIFNIGDCHIYTRHIDNLNIQMKREQY
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCCC
EAPKLWINPDIKNFYDFTIDDFKLIDYKHGDKLTFEVAV
CCCEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA