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The map label for this gene is hyuB [H]

Identifier: 52785741

GI number: 52785741

Start: 1937798

End: 1939537

Strand: Direct

Name: hyuB [H]

Synonym: BLi01984

Alternate gene names: 52785741

Gene position: 1937798-1939537 (Clockwise)

Preceding gene: 52785740

Following gene: 52785742

Centisome position: 45.89

GC content: 51.61

Gene sequence:

>1740_bases
ATGATCAATCAGGACTTTATCTTATCACAGGTGGTCGGCGGTTCTCTTGATGCGGTTGCCAGGGAAATGAGCGCCACTGT
CACGAGAACGGCCCGTTCACCATTGTTTAACGAAGCGCATGATTTTACGACGGGCGTCTTTGACCTTTCCGGCAAAAAAT
CGCGCCTTGTCGCGCAGGCCCCCGGCTGTACGCTTCACCTTTATGCGGTCGTAGGCGCAGTGGACCATCTATTGGACGCC
TTTCGGTATGATCTTCACCCCGGGGATATTCTCCTGGTGAATGATCCGTATCATGGAGGCTCGCACAGCCTTGACTGGAC
GATCGTCATGCCTGTTTTTTATAATCGCAAGCCGATCCTGCTTCCTGCTGTACGAAGCCATATGGCCGATAATGGCGGTC
CGGTTGCCGGAGGGTACAATCCGCGCTCAAAAGACATTTGGCATGACGGGCTGCGGATTCCGCCGTTAAAAATTTATGAG
CGGGGAGAAAAGCGAAGGGATGTATTCGATTTAATCGTTGCAAACAACCGGCTTTCACATTGGCTTGAAGGCGACTTAAA
TGCGATGATCGGGGCGTGCAAGGTCGCTGCCTCCCGAATCCAGACGATGCTGGATAAGTATGGCGCCGAGAAGGTGATTA
CAGCCATTGACAACCGGATTCAATATACAGAAAACAGGGTTCGCGATGAAATCAGCAGCTGGCCGGACGGCACCTATGTC
GGTGAAACATTCGCTGATCACGACTTTCAAGGCAACCGCGATATTAAGGTTCGCGCAACGGTGACCGTCAAAGGCAGCGA
TCTTCACATTGACTTTACCGGTTCATCCCCGCAAGTAAAAGGCTTCATCAACAGCCCGCTCTCAAATACGACTTCGTTTG
CTTTTGTCGGTATTTCGACTTGCTGTGATGAGGATATTCCGATCAATGAAGGATATATGAATCCGGTAACAGTAACCGCG
CCTATAGGAACAGTTGTGAACCCGCAGCTGCCGGCACCGTGCGGACACGCGACTGCGTGCGTCGGAGCAGAAATAGCCGA
GGCTGTTCTTCAGGCCATTTCACAATGTGCGCCAAAACGGGTCGGAGTGAACGCCCATAAGCTTCCTCTCGCCTATTCTC
ACGGAAAATACGATGACGGCAAGCCGTGGGTGAATCTGAATTTTTACGGCTATACCGGCGGTGCGGGAGCTGCATACGAA
ACGGACGGATGGGGGCTTTATCCTCCTGTTATGACAGGAGTTATTCTTCCATCTATTGAAATGACCGAAGTTCAATACCC
GAGCCGTGTATTAAAGCATGAATATACAAGCGATTTTACGGGTGCGGGCCGATGGAGAGGCACACCCGGGTTGGATGTTC
AAATCCAGCACTTAAAGGAAAGCCGCACAAGCGTCATGATGGCAGGGGGCCGAAACACAACAAAAGGCTTCTGCGGAGCC
GGCGATTCACCAAGCAACAAGGTGATTGTCGGGTATGGAACAGACGAAGCCAGAGAAATCGATGAAACGGCTTTTGAAGT
CAAAATGCCTGTGGGCGGCATCATCCAATTTTACCGTGCAGGCGGCGGAGGCTGGGGAAATCCGCTGGAACGTGAACCGG
AAAAGGTGCTGGACGACGTCTTAAACGAGTTTGTCAGCATTGAATCAGCATTGCAGGATTATGGTGTCGTGATTGACCCT
GAGACACTTGCCATTAACGAAGCTGAAACAAAACGTGTGCGGGCATCCAGGCTTCCATAA

Upstream 100 bases:

>100_bases
CGGGAGACCATGACGAAACCTTTTATGAAAGCACGGTACACCAACATGTGAAAAAAGGCAACCTCCTCGTCTTTACGTAC
TCTCAAAAGGAGGAGCAGCT

Downstream 100 bases:

>100_bases
ATAAAAAAGCTGATCTGAGAGGAATTACTTTTCGGATCAGCTTTTTTTCTTTTCAAGACATCTATGGTAAAAAGAGGATA
ATTGCTTTCGAGAGAGAATG

Product: hypothetical protein

Products: ADP; phosphate; N-carbamoylsarcosine

Alternate protein names: ORF3 [H]

Number of amino acids: Translated: 579; Mature: 579

Protein sequence:

>579_residues
MINQDFILSQVVGGSLDAVAREMSATVTRTARSPLFNEAHDFTTGVFDLSGKKSRLVAQAPGCTLHLYAVVGAVDHLLDA
FRYDLHPGDILLVNDPYHGGSHSLDWTIVMPVFYNRKPILLPAVRSHMADNGGPVAGGYNPRSKDIWHDGLRIPPLKIYE
RGEKRRDVFDLIVANNRLSHWLEGDLNAMIGACKVAASRIQTMLDKYGAEKVITAIDNRIQYTENRVRDEISSWPDGTYV
GETFADHDFQGNRDIKVRATVTVKGSDLHIDFTGSSPQVKGFINSPLSNTTSFAFVGISTCCDEDIPINEGYMNPVTVTA
PIGTVVNPQLPAPCGHATACVGAEIAEAVLQAISQCAPKRVGVNAHKLPLAYSHGKYDDGKPWVNLNFYGYTGGAGAAYE
TDGWGLYPPVMTGVILPSIEMTEVQYPSRVLKHEYTSDFTGAGRWRGTPGLDVQIQHLKESRTSVMMAGGRNTTKGFCGA
GDSPSNKVIVGYGTDEAREIDETAFEVKMPVGGIIQFYRAGGGGWGNPLEREPEKVLDDVLNEFVSIESALQDYGVVIDP
ETLAINEAETKRVRASRLP

Sequences:

>Translated_579_residues
MINQDFILSQVVGGSLDAVAREMSATVTRTARSPLFNEAHDFTTGVFDLSGKKSRLVAQAPGCTLHLYAVVGAVDHLLDA
FRYDLHPGDILLVNDPYHGGSHSLDWTIVMPVFYNRKPILLPAVRSHMADNGGPVAGGYNPRSKDIWHDGLRIPPLKIYE
RGEKRRDVFDLIVANNRLSHWLEGDLNAMIGACKVAASRIQTMLDKYGAEKVITAIDNRIQYTENRVRDEISSWPDGTYV
GETFADHDFQGNRDIKVRATVTVKGSDLHIDFTGSSPQVKGFINSPLSNTTSFAFVGISTCCDEDIPINEGYMNPVTVTA
PIGTVVNPQLPAPCGHATACVGAEIAEAVLQAISQCAPKRVGVNAHKLPLAYSHGKYDDGKPWVNLNFYGYTGGAGAAYE
TDGWGLYPPVMTGVILPSIEMTEVQYPSRVLKHEYTSDFTGAGRWRGTPGLDVQIQHLKESRTSVMMAGGRNTTKGFCGA
GDSPSNKVIVGYGTDEAREIDETAFEVKMPVGGIIQFYRAGGGGWGNPLEREPEKVLDDVLNEFVSIESALQDYGVVIDP
ETLAINEAETKRVRASRLP
>Mature_579_residues
MINQDFILSQVVGGSLDAVAREMSATVTRTARSPLFNEAHDFTTGVFDLSGKKSRLVAQAPGCTLHLYAVVGAVDHLLDA
FRYDLHPGDILLVNDPYHGGSHSLDWTIVMPVFYNRKPILLPAVRSHMADNGGPVAGGYNPRSKDIWHDGLRIPPLKIYE
RGEKRRDVFDLIVANNRLSHWLEGDLNAMIGACKVAASRIQTMLDKYGAEKVITAIDNRIQYTENRVRDEISSWPDGTYV
GETFADHDFQGNRDIKVRATVTVKGSDLHIDFTGSSPQVKGFINSPLSNTTSFAFVGISTCCDEDIPINEGYMNPVTVTA
PIGTVVNPQLPAPCGHATACVGAEIAEAVLQAISQCAPKRVGVNAHKLPLAYSHGKYDDGKPWVNLNFYGYTGGAGAAYE
TDGWGLYPPVMTGVILPSIEMTEVQYPSRVLKHEYTSDFTGAGRWRGTPGLDVQIQHLKESRTSVMMAGGRNTTKGFCGA
GDSPSNKVIVGYGTDEAREIDETAFEVKMPVGGIIQFYRAGGGGWGNPLEREPEKVLDDVLNEFVSIESALQDYGVVIDP
ETLAINEAETKRVRASRLP

Specific function: HyuA and hyuB convert D- and L-substituted hydantoins to corresponding N-carbamyl-amino acids [H]

COG id: COG0146

COG function: function code EQ; N-methylhydantoinase B/acetone carboxylase, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the oxoprolinase family [H]

Homologues:

Organism=Homo sapiens, GI48314820, Length=529, Percent_Identity=26.0869565217391, Blast_Score=139, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI133901900, Length=535, Percent_Identity=22.9906542056075, Blast_Score=121, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI133901902, Length=517, Percent_Identity=22.8239845261122, Blast_Score=114, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6322634, Length=491, Percent_Identity=24.0325865580448, Blast_Score=128, Evalue=2e-30,
Organism=Drosophila melanogaster, GI45550492, Length=363, Percent_Identity=27.5482093663912, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003692 [H]

Pfam domain/function: PF02538 Hydantoinase_B [H]

EC number: 3.5.2.14

Molecular weight: Translated: 63047; Mature: 63047

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINQDFILSQVVGGSLDAVAREMSATVTRTARSPLFNEAHDFTTGVFDLSGKKSRLVAQA
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCEEECCCCCCEEEEEC
PGCTLHLYAVVGAVDHLLDAFRYDLHPGDILLVNDPYHGGSHSLDWTIVMPVFYNRKPIL
CCCEEEHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEEEEEEEEECCCCEE
LPAVRSHMADNGGPVAGGYNPRSKDIWHDGLRIPPLKIYERGEKRRDVFDLIVANNRLSH
HHHHHHHHCCCCCCCCCCCCCCCCCHHHCCCCCCCHHHHHCCCHHHHHHHHHHHCCHHHH
WLEGDLNAMIGACKVAASRIQTMLDKYGAEKVITAIDNRIQYTENRVRDEISSWPDGTYV
HHHCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GETFADHDFQGNRDIKVRATVTVKGSDLHIDFTGSSPQVKGFINSPLSNTTSFAFVGIST
CCHHCCCCCCCCCEEEEEEEEEEECCEEEEEECCCCCCEEEEECCCCCCCCCEEEEEEHH
CCDEDIPINEGYMNPVTVTAPIGTVVNPQLPAPCGHATACVGAEIAEAVLQAISQCAPKR
HCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHH
VGVNAHKLPLAYSHGKYDDGKPWVNLNFYGYTGGAGAAYETDGWGLYPPVMTGVILPSIE
CCCCCEECCEEECCCCCCCCCCEEEEEEEEECCCCCCCEECCCCCCCHHHHHHHHCCCCE
MTEVQYPSRVLKHEYTSDFTGAGRWRGTPGLDVQIQHLKESRTSVMMAGGRNTTKGFCGA
EHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEHHHHHHCCCEEEEECCCCCCCCCCCC
GDSPSNKVIVGYGTDEAREIDETAFEVKMPVGGIIQFYRAGGGGWGNPLEREPEKVLDDV
CCCCCCCEEEEECCCHHHHHHCCCEEEECCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
LNEFVSIESALQDYGVVIDPETLAINEAETKRVRASRLP
HHHHHHHHHHHHHCCEEECCCEEEECCHHHHHHHHCCCC
>Mature Secondary Structure
MINQDFILSQVVGGSLDAVAREMSATVTRTARSPLFNEAHDFTTGVFDLSGKKSRLVAQA
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCEEECCCCCCEEEEEC
PGCTLHLYAVVGAVDHLLDAFRYDLHPGDILLVNDPYHGGSHSLDWTIVMPVFYNRKPIL
CCCEEEHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEEEEEEEEECCCCEE
LPAVRSHMADNGGPVAGGYNPRSKDIWHDGLRIPPLKIYERGEKRRDVFDLIVANNRLSH
HHHHHHHHCCCCCCCCCCCCCCCCCHHHCCCCCCCHHHHHCCCHHHHHHHHHHHCCHHHH
WLEGDLNAMIGACKVAASRIQTMLDKYGAEKVITAIDNRIQYTENRVRDEISSWPDGTYV
HHHCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GETFADHDFQGNRDIKVRATVTVKGSDLHIDFTGSSPQVKGFINSPLSNTTSFAFVGIST
CCHHCCCCCCCCCEEEEEEEEEEECCEEEEEECCCCCCEEEEECCCCCCCCCEEEEEEHH
CCDEDIPINEGYMNPVTVTAPIGTVVNPQLPAPCGHATACVGAEIAEAVLQAISQCAPKR
HCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHH
VGVNAHKLPLAYSHGKYDDGKPWVNLNFYGYTGGAGAAYETDGWGLYPPVMTGVILPSIE
CCCCCEECCEEECCCCCCCCCCEEEEEEEEECCCCCCCEECCCCCCCHHHHHHHHCCCCE
MTEVQYPSRVLKHEYTSDFTGAGRWRGTPGLDVQIQHLKESRTSVMMAGGRNTTKGFCGA
EHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEHHHHHHCCCEEEEECCCCCCCCCCCC
GDSPSNKVIVGYGTDEAREIDETAFEVKMPVGGIIQFYRAGGGGWGNPLEREPEKVLDDV
CCCCCCCEEEEECCCHHHHHHCCCEEEECCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
LNEFVSIESALQDYGVVIDPETLAINEAETKRVRASRLP
HHHHHHHHHHHHHCCEEECCCEEEECCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; N-methylimidazolidine-2,4-dione; H2O

Specific reaction: ATP + N-methylimidazolidine-2,4-dione + 2 H2O = ADP + phosphate + N-carbamoylsarcosine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1732229 [H]