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The map label for this gene is dapG [H]

Identifier: 52785660

GI number: 52785660

Start: 1859984

End: 1861198

Strand: Direct

Name: dapG [H]

Synonym: BLi01901

Alternate gene names: 52785660

Gene position: 1859984-1861198 (Clockwise)

Preceding gene: 52785659

Following gene: 52785661

Centisome position: 44.05

GC content: 51.11

Gene sequence:

>1215_bases
GTGAAAATCATCGTTCAAAAATTCGGCGGAACGTCTGTAAAAGATGACCGCGGCAGAAAGCTTGCCTTAAACCATATTAA
GGAAGCGGTCAATGAAGGATACAAAGCGGTTGTCGTCGTATCGGCCATGGGCCGGAAAGGCGATCCGTATGCGACTGATT
CATTGCTTGATCTGCTTTACGGCGGACAGGATACAATTACCGAGCGTGAGCAGGATATGCTTTTATCATGCGGGGAAACG
ATTTCCTCTGTTGTGTTCGCCAGCATGCTTTTGGAAAACGGGATTAAAGCTGCTGCTTTAACCGGTGCGCAAGCGGGCTT
TTTAACAGACAACAACCATACGAATGCAAAGATTCTCGAAATGAAGCCGGAGCGGCTTGTCAGCATGCTGGCCGACCATG
ATGCGGTCGTCGTCGCAGGCTTCCAAGGCGCGACTGCAAAAGGGGATATCACAACGATCGGCCGCGGCGGAAGCGATACA
TCGGCCGCTGCGCTTGGCGCGGCTCTGGACGCGGAATTTATCGATATTTATACAGATGTCGAAGGCGTCATGACAGCTGA
TCCGCGCATCGTTGAAAAAGCGAAGCCGCTGCCTGTGATTACCTATACGGAAATCTGTAATTTAGCCTATCAGGGGGCCA
AGGTGATCCACCCGAGAGCCGTTGAAATCGCAATGCAGGCGAAAGTGCCGATCCGCGTGCGTTCAACCTATTCAAACGAA
ACCGGTACGCTCGTCACAAGCCATCACAACTCGAAAGCCGGAAGCGACGTTTTTGAGCGGCTCATCACAGGCATCGCCCA
TGTGAAAGACGTGACACAGTTTAAAGTCCCGGCAAAAGAAGGACAATATAACGTACAGACTGAAGTGTTTAAAGCGATGG
CGAATGCGGGCATCAGCGTCGATTTCTTCAATATTACACCAAGTGAAATTGTCTACACGGTGGCTGGAAACATGACAGAC
CGCGCCCACAGCATCCTGAAGGAATTGGGCTATGAACCGGCCGTGACGAGAAATTGCGCGAAAGTGTCAGCGGTTGGCGC
GGGGATTATGGGCGTTCCAGGCGTCACCTCAAAAATCGTTACCGCTTTGTCAGAAAAAGGCATTCCGATCCTGCAGTCTG
CTGACAGCCATACGACGATATGGGTGCTCGTGCACGAGAAAAATATGGCGACGGCCGTAAATGCGCTGCATGAAGTGTTT
GAACTGTCAAAATAA

Upstream 100 bases:

>100_bases
TTTAGTTTAAGATTTCTAACGGGATGTGAGAAGAGAGTGAGGCAAAAATGAAGGCCTGCTCTCTTCTGTTACGTCCGAAA
TCAACTTGGAGTGAAATAAA

Downstream 100 bases:

>100_bases
ATATGATTGAATCATGATGAGGTGAAAAAAATGAACTTCGGAAATATCGCAACCGCGATGGTTACGCCCTTTGACAAAAA
TGAAAATATCGATTTTCAAA

Product: aspartate kinase I

Products: NA

Alternate protein names: Aspartate kinase 1; Aspartokinase I [H]

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MKIIVQKFGGTSVKDDRGRKLALNHIKEAVNEGYKAVVVVSAMGRKGDPYATDSLLDLLYGGQDTITEREQDMLLSCGET
ISSVVFASMLLENGIKAAALTGAQAGFLTDNNHTNAKILEMKPERLVSMLADHDAVVVAGFQGATAKGDITTIGRGGSDT
SAAALGAALDAEFIDIYTDVEGVMTADPRIVEKAKPLPVITYTEICNLAYQGAKVIHPRAVEIAMQAKVPIRVRSTYSNE
TGTLVTSHHNSKAGSDVFERLITGIAHVKDVTQFKVPAKEGQYNVQTEVFKAMANAGISVDFFNITPSEIVYTVAGNMTD
RAHSILKELGYEPAVTRNCAKVSAVGAGIMGVPGVTSKIVTALSEKGIPILQSADSHTTIWVLVHEKNMATAVNALHEVF
ELSK

Sequences:

>Translated_404_residues
MKIIVQKFGGTSVKDDRGRKLALNHIKEAVNEGYKAVVVVSAMGRKGDPYATDSLLDLLYGGQDTITEREQDMLLSCGET
ISSVVFASMLLENGIKAAALTGAQAGFLTDNNHTNAKILEMKPERLVSMLADHDAVVVAGFQGATAKGDITTIGRGGSDT
SAAALGAALDAEFIDIYTDVEGVMTADPRIVEKAKPLPVITYTEICNLAYQGAKVIHPRAVEIAMQAKVPIRVRSTYSNE
TGTLVTSHHNSKAGSDVFERLITGIAHVKDVTQFKVPAKEGQYNVQTEVFKAMANAGISVDFFNITPSEIVYTVAGNMTD
RAHSILKELGYEPAVTRNCAKVSAVGAGIMGVPGVTSKIVTALSEKGIPILQSADSHTTIWVLVHEKNMATAVNALHEVF
ELSK
>Mature_404_residues
MKIIVQKFGGTSVKDDRGRKLALNHIKEAVNEGYKAVVVVSAMGRKGDPYATDSLLDLLYGGQDTITEREQDMLLSCGET
ISSVVFASMLLENGIKAAALTGAQAGFLTDNNHTNAKILEMKPERLVSMLADHDAVVVAGFQGATAKGDITTIGRGGSDT
SAAALGAALDAEFIDIYTDVEGVMTADPRIVEKAKPLPVITYTEICNLAYQGAKVIHPRAVEIAMQAKVPIRVRSTYSNE
TGTLVTSHHNSKAGSDVFERLITGIAHVKDVTQFKVPAKEGQYNVQTEVFKAMANAGISVDFFNITPSEIVYTVAGNMTD
RAHSILKELGYEPAVTRNCAKVSAVGAGIMGVPGVTSKIVTALSEKGIPILQSADSHTTIWVLVHEKNMATAVNALHEVF
ELSK

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aspartokinase family [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=286, Percent_Identity=34.965034965035, Blast_Score=141, Evalue=7e-35,
Organism=Escherichia coli, GI1790455, Length=460, Percent_Identity=25.2173913043478, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1790376, Length=337, Percent_Identity=25.5192878338279, Blast_Score=89, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6320893, Length=368, Percent_Identity=25, Blast_Score=104, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 43077; Mature: 43077

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIIVQKFGGTSVKDDRGRKLALNHIKEAVNEGYKAVVVVSAMGRKGDPYATDSLLDLLY
CEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHH
GGQDTITEREQDMLLSCGETISSVVFASMLLENGIKAAALTGAQAGFLTDNNHTNAKILE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEECCCCCCEEEEE
MKPERLVSMLADHDAVVVAGFQGATAKGDITTIGRGGSDTSAAALGAALDAEFIDIYTDV
ECHHHHHHHHCCCCEEEEEECCCCCCCCCEEEECCCCCCCHHHHHHHHHCHHHHEEEECC
EGVMTADPRIVEKAKPLPVITYTEICNLAYQGAKVIHPRAVEIAMQAKVPIRVRSTYSNE
CCEEECCCHHHHHCCCCCEEEHHHHHHHHHCCCEEECCCEEEEEEECCCCEEEEECCCCC
TGTLVTSHHNSKAGSDVFERLITGIAHVKDVTQFKVPAKEGQYNVQTEVFKAMANAGISV
CCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCEE
DFFNITPSEIVYTVAGNMTDRAHSILKELGYEPAVTRNCAKVSAVGAGIMGVPGVTSKIV
EEECCCHHHEEEEECCCCCHHHHHHHHHCCCCCCHHCCHHHHHHHCCCCCCCCCHHHHHH
TALSEKGIPILQSADSHTTIWVLVHEKNMATAVNALHEVFELSK
HHHHHCCCCEEECCCCCCEEEEEEECCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKIIVQKFGGTSVKDDRGRKLALNHIKEAVNEGYKAVVVVSAMGRKGDPYATDSLLDLLY
CEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHH
GGQDTITEREQDMLLSCGETISSVVFASMLLENGIKAAALTGAQAGFLTDNNHTNAKILE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEECCCCCCEEEEE
MKPERLVSMLADHDAVVVAGFQGATAKGDITTIGRGGSDTSAAALGAALDAEFIDIYTDV
ECHHHHHHHHCCCCEEEEEECCCCCCCCCEEEECCCCCCCHHHHHHHHHCHHHHEEEECC
EGVMTADPRIVEKAKPLPVITYTEICNLAYQGAKVIHPRAVEIAMQAKVPIRVRSTYSNE
CCEEECCCHHHHHCCCCCEEEHHHHHHHHHCCCEEECCCEEEEEEECCCCEEEEECCCCC
TGTLVTSHHNSKAGSDVFERLITGIAHVKDVTQFKVPAKEGQYNVQTEVFKAMANAGISV
CCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCEE
DFFNITPSEIVYTVAGNMTDRAHSILKELGYEPAVTRNCAKVSAVGAGIMGVPGVTSKIV
EEECCCHHHEEEEECCCCCHHHHHHHHHCCCCCCHHCCHHHHHHHCCCCCCCCCHHHHHH
TALSEKGIPILQSADSHTTIWVLVHEKNMATAVNALHEVFELSK
HHHHHCCCCEEECCCCCCEEEEEEECCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8098035; 9384377 [H]