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The map label for this gene is mlpA [H]

Identifier: 52785655

GI number: 52785655

Start: 1855519

End: 1856748

Strand: Direct

Name: mlpA [H]

Synonym: BLi01896

Alternate gene names: 52785655

Gene position: 1855519-1856748 (Clockwise)

Preceding gene: 52785654

Following gene: 52785656

Centisome position: 43.94

GC content: 46.42

Gene sequence:

>1230_bases
TTGATTAAACGGTATACTTGTCAAAATGGGGTAAGAATCGTATTCGAAAACAATCCCACCGTCCGGTCTGTGGCCATCGG
AGTTTGGATTGGAACCGGATCAAGACATGAAACACCCGAAATTAACGGCATTTCGCACTTTCTTGAGCACATGTTTTTCA
AGGGGACAAAAACGCGCACTGCAAGGGACATCGCAGAGTCTTTCGATAGAATCGGCGGACAGGTCAACGCGTTTACCTCA
AAGGAATATACGTGCTATTACGCCAAAGTGCTCGATGAGCATGCAAGCTATGCTCTGGAAGTGCTTTCTGACATGTTCTT
TCATTCATCATTTGACGAAGAAGAATTAAAAAAAGAAAAAAACGTCGTCTATGAAGAGATTAAAATGTATGAGGACACAC
CTGATGACATCGTCCACGATCTCTTGAGCAAGGCGTCATACGGCAGCCATTCCCTCGGCTATCCGATCCTCGGCACAGAG
GAAACACTTGCCGAATTTGACGGAGATTCGCTCAGAAAATATATGAACGAATATTACACGCCTGACCGAGTCGTCATTTC
CATAGCCGGAAATGTGCCGGAAACTTTTATAAAAGAAGCTGAAAAGCATTTCGGTTCATATGAAGCCAAAGGCAAAAGAA
CCGGCATGACAAAACCGGATTTTCACCATGAGAAAATGACGCGCAAAAAGGAAACCGAACAGGCTCACCTCTGCCTCGGC
TTCAACGGGCTGGAAGCAGGCCATCCGGAAATCTACGATTTGATCGTTCTCAATAATATTTTGGGAGGAAGCATGAGCAG
CCGCCTCTTTCAGGATGTCCGCGAAGATAAAGGTCTTGCATATTCCGTGTTCAGCTACCACACTTCCTATGAAGACAGCG
GCATGATGACCATCTACGCGGGAACCGGCGCAAATCAGCTTCAGCTCCTGTCAGAAACGATTCATGAAACACTGCGTGCG
CTGAAAAGCGACGGTATTACACCGAAAGAGCTTGAAAACAGCAAAGAGCAAATGAAAGGCAGCCTGATGCTCAGTCTCGA
AAGCACAAACAGCAAAATGAGCCGCAACGGCAAAAATGAGCTTTTGCTCGGTAAACACCGGACGCTTGATGAAATCATCG
AAAAGTTGAATGCCGTCAGCCTCGAGCGTGTGAACAATCTTGCCAACCGGATTTTCACCGATGATTACTCATCTGCATTA
ATCAGCCCGTCGGGTGAGCTGCCGAAATAA

Upstream 100 bases:

>100_bases
AAAAGGCTATCAAATCGGAACTGTTTCACAGCTGATGAATGAAAAAAGGCTTGTTGAAGAATAGGATGTGCGGCCCGTTC
TTTGAATAGGAGGAACAAAT

Downstream 100 bases:

>100_bases
TGCCTCCTTAAAACATGCTTGTCTTTTTAGGCAGGCATGTTTTTTGTTATCGGTTCATACATCATAACAAAAGGGGGTTT
TTTTCATGAGGCTGAGTGAA

Product: MlpA

Products: NA

Alternate protein names: ORFP [H]

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MIKRYTCQNGVRIVFENNPTVRSVAIGVWIGTGSRHETPEINGISHFLEHMFFKGTKTRTARDIAESFDRIGGQVNAFTS
KEYTCYYAKVLDEHASYALEVLSDMFFHSSFDEEELKKEKNVVYEEIKMYEDTPDDIVHDLLSKASYGSHSLGYPILGTE
ETLAEFDGDSLRKYMNEYYTPDRVVISIAGNVPETFIKEAEKHFGSYEAKGKRTGMTKPDFHHEKMTRKKETEQAHLCLG
FNGLEAGHPEIYDLIVLNNILGGSMSSRLFQDVREDKGLAYSVFSYHTSYEDSGMMTIYAGTGANQLQLLSETIHETLRA
LKSDGITPKELENSKEQMKGSLMLSLESTNSKMSRNGKNELLLGKHRTLDEIIEKLNAVSLERVNNLANRIFTDDYSSAL
ISPSGELPK

Sequences:

>Translated_409_residues
MIKRYTCQNGVRIVFENNPTVRSVAIGVWIGTGSRHETPEINGISHFLEHMFFKGTKTRTARDIAESFDRIGGQVNAFTS
KEYTCYYAKVLDEHASYALEVLSDMFFHSSFDEEELKKEKNVVYEEIKMYEDTPDDIVHDLLSKASYGSHSLGYPILGTE
ETLAEFDGDSLRKYMNEYYTPDRVVISIAGNVPETFIKEAEKHFGSYEAKGKRTGMTKPDFHHEKMTRKKETEQAHLCLG
FNGLEAGHPEIYDLIVLNNILGGSMSSRLFQDVREDKGLAYSVFSYHTSYEDSGMMTIYAGTGANQLQLLSETIHETLRA
LKSDGITPKELENSKEQMKGSLMLSLESTNSKMSRNGKNELLLGKHRTLDEIIEKLNAVSLERVNNLANRIFTDDYSSAL
ISPSGELPK
>Mature_409_residues
MIKRYTCQNGVRIVFENNPTVRSVAIGVWIGTGSRHETPEINGISHFLEHMFFKGTKTRTARDIAESFDRIGGQVNAFTS
KEYTCYYAKVLDEHASYALEVLSDMFFHSSFDEEELKKEKNVVYEEIKMYEDTPDDIVHDLLSKASYGSHSLGYPILGTE
ETLAEFDGDSLRKYMNEYYTPDRVVISIAGNVPETFIKEAEKHFGSYEAKGKRTGMTKPDFHHEKMTRKKETEQAHLCLG
FNGLEAGHPEIYDLIVLNNILGGSMSSRLFQDVREDKGLAYSVFSYHTSYEDSGMMTIYAGTGANQLQLLSETIHETLRA
LKSDGITPKELENSKEQMKGSLMLSLESTNSKMSRNGKNELLLGKHRTLDEIIEKLNAVSLERVNNLANRIFTDDYSSAL
ISPSGELPK

Specific function: Unknown

COG id: COG0612

COG function: function code R; Predicted Zn-dependent peptidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M16 family [H]

Homologues:

Organism=Homo sapiens, GI94538354, Length=424, Percent_Identity=28.5377358490566, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI46593007, Length=415, Percent_Identity=24.0963855421687, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI24308013, Length=437, Percent_Identity=24.9427917620137, Blast_Score=125, Evalue=8e-29,
Organism=Homo sapiens, GI50592988, Length=417, Percent_Identity=20.1438848920863, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI155969707, Length=116, Percent_Identity=36.2068965517241, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI1787770, Length=210, Percent_Identity=24.7619047619048, Blast_Score=76, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI71999683, Length=409, Percent_Identity=26.6503667481663, Blast_Score=160, Evalue=9e-40,
Organism=Caenorhabditis elegans, GI17553678, Length=424, Percent_Identity=23.8207547169811, Blast_Score=145, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI17510601, Length=416, Percent_Identity=22.3557692307692, Blast_Score=91, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323192, Length=399, Percent_Identity=29.0726817042606, Blast_Score=185, Evalue=9e-48,
Organism=Saccharomyces cerevisiae, GI6321813, Length=431, Percent_Identity=25.5220417633411, Blast_Score=150, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6319426, Length=411, Percent_Identity=22.3844282238443, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21357875, Length=413, Percent_Identity=28.8135593220339, Blast_Score=175, Evalue=6e-44,
Organism=Drosophila melanogaster, GI24646943, Length=413, Percent_Identity=28.8135593220339, Blast_Score=175, Evalue=6e-44,
Organism=Drosophila melanogaster, GI19921772, Length=432, Percent_Identity=22.4537037037037, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI24665395, Length=406, Percent_Identity=20.1970443349754, Blast_Score=70, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011249
- InterPro:   IPR011237
- InterPro:   IPR011765
- InterPro:   IPR001431
- InterPro:   IPR007863 [H]

Pfam domain/function: PF00675 Peptidase_M16; PF05193 Peptidase_M16_C [H]

EC number: 3.4.99.- [C]

Molecular weight: Translated: 46221; Mature: 46221

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00143 INSULINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKRYTCQNGVRIVFENNPTVRSVAIGVWIGTGSRHETPEINGISHFLEHMFFKGTKTRT
CCCCEECCCCCEEEECCCCCEEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCHH
ARDIAESFDRIGGQVNAFTSKEYTCYYAKVLDEHASYALEVLSDMFFHSSFDEEELKKEK
HHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
NVVYEEIKMYEDTPDDIVHDLLSKASYGSHSLGYPILGTEETLAEFDGDSLRKYMNEYYT
HHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHCC
PDRVVISIAGNVPETFIKEAEKHFGSYEAKGKRTGMTKPDFHHEKMTRKKETEQAHLCLG
CCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEE
FNGLEAGHPEIYDLIVLNNILGGSMSSRLFQDVREDKGLAYSVFSYHTSYEDSGMMTIYA
CCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEHHCCCCCCCCEEEEEE
GTGANQLQLLSETIHETLRALKSDGITPKELENSKEQMKGSLMLSLESTNSKMSRNGKNE
CCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCHHHHCCCEEEEEEECCCCHHHCCCCCC
LLLGKHRTLDEIIEKLNAVSLERVNNLANRIFTDDYSSALISPSGELPK
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEECCCCCCCC
>Mature Secondary Structure
MIKRYTCQNGVRIVFENNPTVRSVAIGVWIGTGSRHETPEINGISHFLEHMFFKGTKTRT
CCCCEECCCCCEEEECCCCCEEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCHH
ARDIAESFDRIGGQVNAFTSKEYTCYYAKVLDEHASYALEVLSDMFFHSSFDEEELKKEK
HHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
NVVYEEIKMYEDTPDDIVHDLLSKASYGSHSLGYPILGTEETLAEFDGDSLRKYMNEYYT
HHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHCC
PDRVVISIAGNVPETFIKEAEKHFGSYEAKGKRTGMTKPDFHHEKMTRKKETEQAHLCLG
CCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEE
FNGLEAGHPEIYDLIVLNNILGGSMSSRLFQDVREDKGLAYSVFSYHTSYEDSGMMTIYA
CCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEHHCCCCCCCCEEEEEE
GTGANQLQLLSETIHETLRALKSDGITPKELENSKEQMKGSLMLSLESTNSKMSRNGKNE
CCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCHHHHCCCEEEEEEECCCCHHHCCCCCC
LLLGKHRTLDEIIEKLNAVSLERVNNLANRIFTDDYSSALISPSGELPK
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Zn [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Endopeptidases of unknown catalytic mechanism [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 8098035 [H]