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The map label for this gene is pnpA [H]

Identifier: 52785653

GI number: 52785653

Start: 1852256

End: 1854373

Strand: Direct

Name: pnpA [H]

Synonym: BLi01894

Alternate gene names: 52785653

Gene position: 1852256-1854373 (Clockwise)

Preceding gene: 52785652

Following gene: 52785654

Centisome position: 43.86

GC content: 49.2

Gene sequence:

>2118_bases
ATGGGACAAGAGAAACATGTCTTTTCCATAGATTGGGCCGGCCGCAAGCTCACAGTAGAAACCGGCCAGCTTGCAAAACA
AGCAAACGGAGCTGTCATGGTGCGTTATGGAGACACTGCAGTGCTCAGCACCGCAACGGCTTCAAAAGAACCTAAAACCG
TAGACTTTTTCCCGTTAACCGTAAATTACGAAGAAAGACTTTACGCCGTCGGAAAGATTCCGGGCGGGTTTATTAAAAGA
GAGGGAAGACCGAGCGAAAAAGCGATTCTCGCCAGCCGGTTAATCGACAGGCCGATCCGCCCGCTGTTCGCAGACGGCTT
CCGGAATGAAGTTCAAGTCGTCAGCATCGTCATGAGCGTTGACCAGGATTGTTCGTCAGAAATGGCGGCAATGTTTGGAT
CATCTCTTGCGCTGTGCGTATCCGACATCCCGTTTGAAGGGCCGATCGCCGGAGTGACGGTAGGCCGGGTTGACAACAAA
TTCATCATCAATCCGACACTTGAACAGCTTGAAAAAAGCGACATTCATCTCGTCGTAGCGGGAACGAAGGACGCGATCAA
CATGGTTGAAGCGGGAGCAGACGAAGTTCCGGAAGAGATCATGCTTGAAGCGATCATGTTCGGTCACGAGGAGATCAAAC
GGCTGATTGCCTTCCAGGAGGAAATCGTCGCCGCTGTCGGGAAAGAAAAATCAGAAATCACCCTGTATGAGATCGATTCC
GATCTGAAAGAAAAAGTGCGGGGAATGGCCGAATCGAACCTCTTAAAAGCCATTCAGGTTCATGAAAAGCATGCGCGCGA
GGACGCGATCAGCGAAGTGAAAAACGAGGTTTTGGCCAAGTTTGAAGAAGAGGAACACGATGAAGAAACGCTGAAACAAG
TGAAAGACATTTTGTCCCAGCTTGTTAAAAATGAAGTGCGCCGTCTGATTACGGAGGAAAAAGTTCGTCCTGACGGCAGG
GCTGTCGATGAGATCCGCCCGCTTTCTTCAGAAGTCGGACTGCTTCCGAGAACACACGGTTCAGGACTTTTTACAAGGGG
ACAGACTCAGGCGCTCAGCATTTGTACGCTCGGTGCGCTTGGCGATGTGCAGATTTTAGACGGTCTCGGCGTCGAAGAAT
CCAAACGCTTTATGCACCATTACAACTTCCCGCAGTTCAGCGTCGGGGAAACAGGGCCGATGCGCGGGCCGGGACGCCGC
GAAATCGGGCACGGAGCCCTCGGGGAACGAGCGCTTGAGCCGATTGTTCCGTCTGAAAAAGACTTCCCGTACACCATCCG
CCTCGTCTCTGAAGTGCTTGAATCAAACGGTTCCACTTCCCAGGCGAGCATTTGTGCAAGCACGCTAGCGATGATGGATG
CAGGGGTGCCGATTAAAGCCCCGGTTGCCGGAATCGCGATGGGTCTTGTCAAATCAGGTGACAACTATACCGTCTTAACC
GATATTCAAGGTATGGAAGACCATCTCGGGGATATGGACTTTAAAGTGGCGGGAACGTCCAAAGGCGTCACAGCTCTGCA
AATGGATATTAAGATCGAAGGCTTATCGAGAGAGATTTTGGAAGAGGCTCTTCAGCAGGCGAAAAAAGGCAGAATGGAGA
TCCTTGAAAGCATGCTGAGCACATTGGCTGAATCAAGAAAAGAGCTTTCTCCTTATGCGCCGAAAATCTTGACGATGTCG
ATCAATCCGGATAAAATCCGCGACGTCATCGGACCAAGCGGAAAACAAATCAATAAGATCATTGAAGATACAGGCGTTAA
AATCGATATTGAACAAGACGGCACAATCTTCATTTCTTCTACCGATGAAAGCATGAACCAAAAAGCGAAGAAAATCATCG
AAGACCTTGTCAGAGAAGTTGAAGTCGGACAGCTTTACTTAGGTAAAGTGAAGCGGATTGAAAAATTCGGAGCTTTCGTT
GAACTGTTCAGCGGAAAAGACGGTCTTGTCCATATTTCAGAGCTTGCGCTCGAACGGGTAGGAAAAGTCGAAGATGTCGT
CAAAATCGGCGACGAGCTCCTTGTAAAGGTTACTGAAATCGATAAACAAGGACGCGTCAACTTATCAAGAAAAGCTGTAC
TGCGTGAACAGAAGGAAAAAGAAGAACAAAAATCGTAA

Upstream 100 bases:

>100_bases
TTCATTCTTTACATAGCACTTTGCCATTGATATGTTCAATTCATTTCGTTCATAATAGGAGTAACCTAAAAACTATCCGA
AGAGAGGAGTTCATATAAGA

Downstream 100 bases:

>100_bases
AAAAAACGGTGTCTATCTTGCTTCTGTCTTCGTCTTCAAGCAACAGGAAGCCTGGGTAACCCGGCTTCTTTGTTTTAAGC
CGTGAAAACGGTCCGGTTCT

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 705; Mature: 704

Protein sequence:

>705_residues
MGQEKHVFSIDWAGRKLTVETGQLAKQANGAVMVRYGDTAVLSTATASKEPKTVDFFPLTVNYEERLYAVGKIPGGFIKR
EGRPSEKAILASRLIDRPIRPLFADGFRNEVQVVSIVMSVDQDCSSEMAAMFGSSLALCVSDIPFEGPIAGVTVGRVDNK
FIINPTLEQLEKSDIHLVVAGTKDAINMVEAGADEVPEEIMLEAIMFGHEEIKRLIAFQEEIVAAVGKEKSEITLYEIDS
DLKEKVRGMAESNLLKAIQVHEKHAREDAISEVKNEVLAKFEEEEHDEETLKQVKDILSQLVKNEVRRLITEEKVRPDGR
AVDEIRPLSSEVGLLPRTHGSGLFTRGQTQALSICTLGALGDVQILDGLGVEESKRFMHHYNFPQFSVGETGPMRGPGRR
EIGHGALGERALEPIVPSEKDFPYTIRLVSEVLESNGSTSQASICASTLAMMDAGVPIKAPVAGIAMGLVKSGDNYTVLT
DIQGMEDHLGDMDFKVAGTSKGVTALQMDIKIEGLSREILEEALQQAKKGRMEILESMLSTLAESRKELSPYAPKILTMS
INPDKIRDVIGPSGKQINKIIEDTGVKIDIEQDGTIFISSTDESMNQKAKKIIEDLVREVEVGQLYLGKVKRIEKFGAFV
ELFSGKDGLVHISELALERVGKVEDVVKIGDELLVKVTEIDKQGRVNLSRKAVLREQKEKEEQKS

Sequences:

>Translated_705_residues
MGQEKHVFSIDWAGRKLTVETGQLAKQANGAVMVRYGDTAVLSTATASKEPKTVDFFPLTVNYEERLYAVGKIPGGFIKR
EGRPSEKAILASRLIDRPIRPLFADGFRNEVQVVSIVMSVDQDCSSEMAAMFGSSLALCVSDIPFEGPIAGVTVGRVDNK
FIINPTLEQLEKSDIHLVVAGTKDAINMVEAGADEVPEEIMLEAIMFGHEEIKRLIAFQEEIVAAVGKEKSEITLYEIDS
DLKEKVRGMAESNLLKAIQVHEKHAREDAISEVKNEVLAKFEEEEHDEETLKQVKDILSQLVKNEVRRLITEEKVRPDGR
AVDEIRPLSSEVGLLPRTHGSGLFTRGQTQALSICTLGALGDVQILDGLGVEESKRFMHHYNFPQFSVGETGPMRGPGRR
EIGHGALGERALEPIVPSEKDFPYTIRLVSEVLESNGSTSQASICASTLAMMDAGVPIKAPVAGIAMGLVKSGDNYTVLT
DIQGMEDHLGDMDFKVAGTSKGVTALQMDIKIEGLSREILEEALQQAKKGRMEILESMLSTLAESRKELSPYAPKILTMS
INPDKIRDVIGPSGKQINKIIEDTGVKIDIEQDGTIFISSTDESMNQKAKKIIEDLVREVEVGQLYLGKVKRIEKFGAFV
ELFSGKDGLVHISELALERVGKVEDVVKIGDELLVKVTEIDKQGRVNLSRKAVLREQKEKEEQKS
>Mature_704_residues
GQEKHVFSIDWAGRKLTVETGQLAKQANGAVMVRYGDTAVLSTATASKEPKTVDFFPLTVNYEERLYAVGKIPGGFIKRE
GRPSEKAILASRLIDRPIRPLFADGFRNEVQVVSIVMSVDQDCSSEMAAMFGSSLALCVSDIPFEGPIAGVTVGRVDNKF
IINPTLEQLEKSDIHLVVAGTKDAINMVEAGADEVPEEIMLEAIMFGHEEIKRLIAFQEEIVAAVGKEKSEITLYEIDSD
LKEKVRGMAESNLLKAIQVHEKHAREDAISEVKNEVLAKFEEEEHDEETLKQVKDILSQLVKNEVRRLITEEKVRPDGRA
VDEIRPLSSEVGLLPRTHGSGLFTRGQTQALSICTLGALGDVQILDGLGVEESKRFMHHYNFPQFSVGETGPMRGPGRRE
IGHGALGERALEPIVPSEKDFPYTIRLVSEVLESNGSTSQASICASTLAMMDAGVPIKAPVAGIAMGLVKSGDNYTVLTD
IQGMEDHLGDMDFKVAGTSKGVTALQMDIKIEGLSREILEEALQQAKKGRMEILESMLSTLAESRKELSPYAPKILTMSI
NPDKIRDVIGPSGKQINKIIEDTGVKIDIEQDGTIFISSTDESMNQKAKKIIEDLVREVEVGQLYLGKVKRIEKFGAFVE
LFSGKDGLVHISELALERVGKVEDVVKIGDELLVKVTEIDKQGRVNLSRKAVLREQKEKEEQKS

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=708, Percent_Identity=35.3107344632768, Blast_Score=429, Evalue=1e-120,
Organism=Escherichia coli, GI145693187, Length=688, Percent_Identity=50.2906976744186, Blast_Score=667, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=726, Percent_Identity=33.8842975206612, Blast_Score=352, Evalue=4e-97,
Organism=Drosophila melanogaster, GI281362905, Length=706, Percent_Identity=38.385269121813, Blast_Score=458, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24651641, Length=706, Percent_Identity=38.385269121813, Blast_Score=458, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24651643, Length=706, Percent_Identity=38.385269121813, Blast_Score=458, Evalue=1e-129,
Organism=Drosophila melanogaster, GI161079377, Length=651, Percent_Identity=37.9416282642089, Blast_Score=418, Evalue=1e-117,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 77534; Mature: 77403

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQEKHVFSIDWAGRKLTVETGQLAKQANGAVMVRYGDTAVLSTATASKEPKTVDFFPLT
CCCCCCEEEEECCCCEEEEEHHHHHHHCCCEEEEEECCCEEEEECCCCCCCCEEEEEEEE
VNYEERLYAVGKIPGGFIKREGRPSEKAILASRLIDRPIRPLFADGFRNEVQVVSIVMSV
ECHHHHEEEEECCCCCHHCCCCCCHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHH
DQDCSSEMAAMFGSSLALCVSDIPFEGPIAGVTVGRVDNKFIINPTLEQLEKSDIHLVVA
CCCHHHHHHHHHCCCHHHHHHCCCCCCCCCCEEEECCCCEEEECCHHHHHCCCCEEEEEE
GTKDAINMVEAGADEVPEEIMLEAIMFGHEEIKRLIAFQEEIVAAVGKEKSEITLYEIDS
CCHHHHHHHHCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCH
DLKEKVRGMAESNLLKAIQVHEKHAREDAISEVKNEVLAKFEEEEHDEETLKQVKDILSQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
LVKNEVRRLITEEKVRPDGRAVDEIRPLSSEVGLLPRTHGSGLFTRGQTQALSICTLGAL
HHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHCCCCCCCCCCCCEECCCCCEEEEEEECCC
GDVQILDGLGVEESKRFMHHYNFPQFSVGETGPMRGPGRREIGHGALGERALEPIVPSEK
CCCHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCC
DFPYTIRLVSEVLESNGSTSQASICASTLAMMDAGVPIKAPVAGIAMGLVKSGDNYTVLT
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEEEE
DIQGMEDHLGDMDFKVAGTSKGVTALQMDIKIEGLSREILEEALQQAKKGRMEILESMLS
ECCCHHHHCCCCCEEEECCCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHH
TLAESRKELSPYAPKILTMSINPDKIRDVIGPSGKQINKIIEDTGVKIDIEQDGTIFISS
HHHHHHHHCCCCCCEEEEEECCHHHHHHHHCCCHHHHHHHHHHCCCEEEEECCCEEEEEC
TDESMNQKAKKIIEDLVREVEVGQLYLGKVKRIEKFGAFVELFSGKDGLVHISELALERV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHH
GKVEDVVKIGDELLVKVTEIDKQGRVNLSRKAVLREQKEKEEQKS
CCHHHHHHCCHHHEEEHHHCCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GQEKHVFSIDWAGRKLTVETGQLAKQANGAVMVRYGDTAVLSTATASKEPKTVDFFPLT
CCCCCEEEEECCCCEEEEEHHHHHHHCCCEEEEEECCCEEEEECCCCCCCCEEEEEEEE
VNYEERLYAVGKIPGGFIKREGRPSEKAILASRLIDRPIRPLFADGFRNEVQVVSIVMSV
ECHHHHEEEEECCCCCHHCCCCCCHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHH
DQDCSSEMAAMFGSSLALCVSDIPFEGPIAGVTVGRVDNKFIINPTLEQLEKSDIHLVVA
CCCHHHHHHHHHCCCHHHHHHCCCCCCCCCCEEEECCCCEEEECCHHHHHCCCCEEEEEE
GTKDAINMVEAGADEVPEEIMLEAIMFGHEEIKRLIAFQEEIVAAVGKEKSEITLYEIDS
CCHHHHHHHHCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCH
DLKEKVRGMAESNLLKAIQVHEKHAREDAISEVKNEVLAKFEEEEHDEETLKQVKDILSQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
LVKNEVRRLITEEKVRPDGRAVDEIRPLSSEVGLLPRTHGSGLFTRGQTQALSICTLGAL
HHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHCCCCCCCCCCCCEECCCCCEEEEEEECCC
GDVQILDGLGVEESKRFMHHYNFPQFSVGETGPMRGPGRREIGHGALGERALEPIVPSEK
CCCHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCC
DFPYTIRLVSEVLESNGSTSQASICASTLAMMDAGVPIKAPVAGIAMGLVKSGDNYTVLT
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEEEE
DIQGMEDHLGDMDFKVAGTSKGVTALQMDIKIEGLSREILEEALQQAKKGRMEILESMLS
ECCCHHHHCCCCCEEEECCCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHH
TLAESRKELSPYAPKILTMSINPDKIRDVIGPSGKQINKIIEDTGVKIDIEQDGTIFISS
HHHHHHHHCCCCCCEEEEEECCHHHHHHHHCCCHHHHHHHHHHCCCEEEEECCCEEEEEC
TDESMNQKAKKIIEDLVREVEVGQLYLGKVKRIEKFGAFVELFSGKDGLVHISELALERV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHH
GKVEDVVKIGDELLVKVTEIDKQGRVNLSRKAVLREQKEKEEQKS
CCHHHHHHCCHHHEEEHHHCCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA