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The map label for this gene is yxjB [H]

Identifier: 52785513

GI number: 52785513

Start: 1713361

End: 1713990

Strand: Direct

Name: yxjB [H]

Synonym: BLi01754

Alternate gene names: 52785513

Gene position: 1713361-1713990 (Clockwise)

Preceding gene: 52785512

Following gene: 52785514

Centisome position: 40.58

GC content: 51.9

Gene sequence:

>630_bases
TTGACAGACCGTCTTAAACAAGCCGGCCTGCCGTTTTGCAATTTTGTCAGGATCCTCGATGCCGGCTGCGGGGAAGGTTC
GCATTTGACGCGAATCGCTGAAACGCTAGAAGCGCGTTTCGGAACGCCTGTCCTTGGAGTCGGGCTGGATTTGGCGAAAG
CCGGGATTGTATCGGCGGCTAAGCAAGAAAGAGAGGCGATCTGGTGTGTCGCCGATCTGGCTGCCGCACCGTTTCGGAAT
CGTTCATTCGACGCGATCCTCAATATATTGTCGCCTTCTAATTACGCGGAATTCGAGCGGCTGTTATCGGATGACGGCAT
CGTTTTGAAAGTGATTCCGAGAAGCGGCCATCTGAAAGAACTGAGGAGATTTTTGTACGACGGCTCGGACAAACAAGCCT
ATACGAGCGAGGATACGGCTGAGCGCTTCGCGTCTCATTTTGATCTGATCGACAGAACGGAGATCGCCTACAGCAAACGT
TTAGACCGAATCCAGCTCCCGCCGCTCATCAAGATGACGCCTTTATCATGGCGCGCGCAAAAGGAGCGGATCAATGCCTT
TCTAAAGAAGGAATCCGCTGTTATGACCGTCCATCTCGATATTTTAGCCGGCAGGAAAAAAACGAAATAA

Upstream 100 bases:

>100_bases
GGTATGTCAATGTCGCGACGCATTCTCCTTCCGTTAAGATTTATTTGAAGCCAGAAGAATGTTAGCAGCCGCCGGCTTTT
TTGAACCGATGAGAGGCGCG

Downstream 100 bases:

>100_bases
ACCACTGAAAAGGCAGTCTAAATAAAGGACTGCCTTTCATATAATCTTAAAAGGACATTTGATCAGAGGAGGCGTGCGGT
GGACATGATGAATATTTCTG

Product: YxjB

Products: S-adenosyl-L-homocysteine +rRNA containing Nmethylguanine [C]

Alternate protein names: NA

Number of amino acids: Translated: 209; Mature: 208

Protein sequence:

>209_residues
MTDRLKQAGLPFCNFVRILDAGCGEGSHLTRIAETLEARFGTPVLGVGLDLAKAGIVSAAKQEREAIWCVADLAAAPFRN
RSFDAILNILSPSNYAEFERLLSDDGIVLKVIPRSGHLKELRRFLYDGSDKQAYTSEDTAERFASHFDLIDRTEIAYSKR
LDRIQLPPLIKMTPLSWRAQKERINAFLKKESAVMTVHLDILAGRKKTK

Sequences:

>Translated_209_residues
MTDRLKQAGLPFCNFVRILDAGCGEGSHLTRIAETLEARFGTPVLGVGLDLAKAGIVSAAKQEREAIWCVADLAAAPFRN
RSFDAILNILSPSNYAEFERLLSDDGIVLKVIPRSGHLKELRRFLYDGSDKQAYTSEDTAERFASHFDLIDRTEIAYSKR
LDRIQLPPLIKMTPLSWRAQKERINAFLKKESAVMTVHLDILAGRKKTK
>Mature_208_residues
TDRLKQAGLPFCNFVRILDAGCGEGSHLTRIAETLEARFGTPVLGVGLDLAKAGIVSAAKQEREAIWCVADLAAAPFRNR
SFDAILNILSPSNYAEFERLLSDDGIVLKVIPRSGHLKELRRFLYDGSDKQAYTSEDTAERFASHFDLIDRTEIAYSKRL
DRIQLPPLIKMTPLSWRAQKERINAFLKKESAVMTVHLDILAGRKKTK

Specific function: Specifically Methylates The Guanosine In Position 745 Of 23s rRNA. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmA family [H]

Homologues:

Organism=Escherichia coli, GI1788125, Length=174, Percent_Identity=32.183908045977, Blast_Score=91, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013216
- InterPro:   IPR016718 [H]

Pfam domain/function: PF08241 Methyltransf_11 [H]

EC number: 2.1.1.51 [C]

Molecular weight: Translated: 23418; Mature: 23287

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDRLKQAGLPFCNFVRILDAGCGEGSHLTRIAETLEARFGTPVLGVGLDLAKAGIVSAA
CCCCCHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHH
KQEREAIWCVADLAAAPFRNRSFDAILNILSPSNYAEFERLLSDDGIVLKVIPRSGHLKE
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHCCCCEEEEEECCCCCHHH
LRRFLYDGSDKQAYTSEDTAERFASHFDLIDRTEIAYSKRLDRIQLPPLIKMTPLSWRAQ
HHHHHHCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCEEECCCCHHHH
KERINAFLKKESAVMTVHLDILAGRKKTK
HHHHHHHHHHCCEEEEEEEEEECCCCCCC
>Mature Secondary Structure 
TDRLKQAGLPFCNFVRILDAGCGEGSHLTRIAETLEARFGTPVLGVGLDLAKAGIVSAA
CCCCHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHH
KQEREAIWCVADLAAAPFRNRSFDAILNILSPSNYAEFERLLSDDGIVLKVIPRSGHLKE
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHCCCCEEEEEECCCCCHHH
LRRFLYDGSDKQAYTSEDTAERFASHFDLIDRTEIAYSKRLDRIQLPPLIKMTPLSWRAQ
HHHHHHCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCEEECCCCHHHH
KERINAFLKKESAVMTVHLDILAGRKKTK
HHHHHHHHHHCCEEEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Unidentified protein factor B [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; rRNA [C]

Specific reaction: S-Adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine +rRNA containing Nmethylguanine [C]

General reaction: Methyl group transfer [C]

Inhibitor: Mg2+; Unidentified protein factor A1 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969509; 9384377 [H]