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The map label for this gene is sigE [H]

Identifier: 52785509

GI number: 52785509

Start: 1710274

End: 1710993

Strand: Direct

Name: sigE [H]

Synonym: BLi01750

Alternate gene names: 52785509

Gene position: 1710274-1710993 (Clockwise)

Preceding gene: 52785508

Following gene: 52785510

Centisome position: 40.5

GC content: 41.39

Gene sequence:

>720_bases
ATGAAAAAACTAAAATTAAGGTTAACCTATCTATGGTACAAACTTTTAATGAAACTGGGGCTGAAAAGCGACGAAATTTA
TTATATCGGCGGAAGCGAGGCGCTTCCCCCGCCATTGTCAAAAGATGAAGAGCAGGTGCTTCTCCATAAGCTGCCTGACG
GTGATCAGGCGGCACGAGCGATTTTGATTGAACGAAATCTCAGACTGGTCGTGTACATCGCGAGAAAATTTGAAAATACA
GGAATCAATATCGAGGATTTAATCTCCATCGGCACGATCGGGCTCATCAAAGCGGTGAATACGTTTAATCCCGAGAAAAA
AATCAAACTGGCTACATATGCTTCCAGATGCATTGAAAATGAAATTTTGATGTATTTAAGAAGAAACAATAAAATCCGTT
CAGAGGTATCATTCGACGAACCGCTGAACATCGATTGGGACGGAAATGAGCTGCTTCTCTCAGATGTGCTCGGAACAGAA
AACGACATTATTACAAAAGACATCGAAGCAAACGTTGACAAGAAGCTGCTGAAAAAAGCGCTTGAACAGCTCAATGACAG
AGAAAAACAGATCATGGAGCTCCGTTTCGGGCTTGTCGGAGGGGAAGAAAAAACACAAAAAGACGTTGCCGATATGCTCG
GGATCTCCCAGTCATATATTTCCCGACTTGAAAAAAGAATTATTAAAAGGCTCCGAAAAGAATTCAACAAGATGGTTTAA

Upstream 100 bases:

>100_bases
CGAAAATGCTGGCCGGAAACCCGGTCAAACACGTTTCTTAAACTTGAAGTCTGTTACATTATCATACTCCTGAAGACGTT
TATTTAGAAGGGGGAGGAAG

Downstream 100 bases:

>100_bases
TTTTTTTATGAAATTTGAAGCATTGACAGTCAAGGGTTTTCGGCTCGATCTTCTTATTTTTGGAAATTAACGATGCATAT
TTTTCCCACCCGAGGAGATA

Product: sporulation sigma factor SigE

Products: NA

Alternate protein names: P31; Sigma-29; Stage II sporulation protein GB [H]

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MKKLKLRLTYLWYKLLMKLGLKSDEIYYIGGSEALPPPLSKDEEQVLLHKLPDGDQAARAILIERNLRLVVYIARKFENT
GINIEDLISIGTIGLIKAVNTFNPEKKIKLATYASRCIENEILMYLRRNNKIRSEVSFDEPLNIDWDGNELLLSDVLGTE
NDIITKDIEANVDKKLLKKALEQLNDREKQIMELRFGLVGGEEKTQKDVADMLGISQSYISRLEKRIIKRLRKEFNKMV

Sequences:

>Translated_239_residues
MKKLKLRLTYLWYKLLMKLGLKSDEIYYIGGSEALPPPLSKDEEQVLLHKLPDGDQAARAILIERNLRLVVYIARKFENT
GINIEDLISIGTIGLIKAVNTFNPEKKIKLATYASRCIENEILMYLRRNNKIRSEVSFDEPLNIDWDGNELLLSDVLGTE
NDIITKDIEANVDKKLLKKALEQLNDREKQIMELRFGLVGGEEKTQKDVADMLGISQSYISRLEKRIIKRLRKEFNKMV
>Mature_239_residues
MKKLKLRLTYLWYKLLMKLGLKSDEIYYIGGSEALPPPLSKDEEQVLLHKLPDGDQAARAILIERNLRLVVYIARKFENT
GINIEDLISIGTIGLIKAVNTFNPEKKIKLATYASRCIENEILMYLRRNNKIRSEVSFDEPLNIDWDGNELLLSDVLGTE
NDIITKDIEANVDKKLLKKALEQLNDREKQIMELRFGLVGGEEKTQKDVADMLGISQSYISRLEKRIIKRLRKEFNKMV

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of sporulation specific genes [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=256, Percent_Identity=29.6875, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1789871, Length=106, Percent_Identity=45.2830188679245, Blast_Score=79, Evalue=2e-16,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014200
- InterPro:   IPR016263
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 27609; Mature: 27609

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLKLRLTYLWYKLLMKLGLKSDEIYYIGGSEALPPPLSKDEEQVLLHKLPDGDQAARA
CCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCHHHHHHHCCCCCHHHHHH
ILIERNLRLVVYIARKFENTGINIEDLISIGTIGLIKAVNTFNPEKKIKLATYASRCIEN
HHHCCCCEEEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
EILMYLRRNNKIRSEVSFDEPLNIDWDGNELLLSDVLGTENDIITKDIEANVDKKLLKKA
HHHHHHHHCCCHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHCCCCHHHHHHH
LEQLNDREKQIMELRFGLVGGEEKTQKDVADMLGISQSYISRLEKRIIKRLRKEFNKMV
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKLKLRLTYLWYKLLMKLGLKSDEIYYIGGSEALPPPLSKDEEQVLLHKLPDGDQAARA
CCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCHHHHHHHCCCCCHHHHHH
ILIERNLRLVVYIARKFENTGINIEDLISIGTIGLIKAVNTFNPEKKIKLATYASRCIEN
HHHCCCCEEEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
EILMYLRRNNKIRSEVSFDEPLNIDWDGNELLLSDVLGTENDIITKDIEANVDKKLLKKA
HHHHHHHHCCCHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHCCCCHHHHHHH
LEQLNDREKQIMELRFGLVGGEEKTQKDVADMLGISQSYISRLEKRIIKRLRKEFNKMV
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6438529; 9384377; 3104904; 2106671 [H]