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The map label for this gene is ykuU [H]

Identifier: 52785396

GI number: 52785396

Start: 1596537

End: 1597079

Strand: Direct

Name: ykuU [H]

Synonym: BLi01635

Alternate gene names: 52785396

Gene position: 1596537-1597079 (Clockwise)

Preceding gene: 52785395

Following gene: 52785397

Centisome position: 37.81

GC content: 46.04

Gene sequence:

>543_bases
ATGGCAGAACGCATGGTAGGTAAACAAGCGCCGCGCTTTGAAATGGAAGCGGTTCTTGCAAACAAAGAATTCGGAAAAGT
AAGCCTTGAAGAAAACATGAAAAATGATAAGTGGACGGTTCTATTCTTCTATCCAATGGACTTCACTTTCGTGTGTCCGA
CAGAAATTACAGCAATGTCTGACCGCTATGACGAGTTTGAAGACCTTGATGCAGAAGTTATCGGCGTATCAACTGACACC
ATCCACACTCACTTGGCATGGATTAACACGGACCGCAAAGATAACGGTCTCGGCCAGCTGAAATATCCGCTTGCTGCCGA
CACAAACCATGAAGTATCTCGTGAATACGGCGTACTGATTGAAGAAGAAGGTGTAGCGCTTCGCGGATTGTTCATCATTA
ATCCAGAAGGCGAACTTCAATATCAAACAGTGTTCCATAACAATATCGGCCGTGATGTAGACGAAACATTGCGCGTGCTT
CAAGCGCTGCAAACAGGCGGTCTTTGCCCTGCAAACTGGAAACCGGGACAAGCAACCCTTTAA

Upstream 100 bases:

>100_bases
ATGTATGTGATATTATTAACTAAATGAGAATTTAATATTTGAATTCTCATTTAGATACGGGTAGCATAACCCAAAAATCA
CAATTGGAGGGATACATAAA

Downstream 100 bases:

>100_bases
TTTCGATAAGAAATCTGATTGGAAAGGTCTAACATTTGTTAGACCTTTTTAAACGACAAAAATGACATTGGAGGAAAAAT
CATGAAATTACGTCAGCCGA

Product: YkuU

Products: an alcohol; H2O; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 180; Mature: 179

Protein sequence:

>180_residues
MAERMVGKQAPRFEMEAVLANKEFGKVSLEENMKNDKWTVLFFYPMDFTFVCPTEITAMSDRYDEFEDLDAEVIGVSTDT
IHTHLAWINTDRKDNGLGQLKYPLAADTNHEVSREYGVLIEEEGVALRGLFIINPEGELQYQTVFHNNIGRDVDETLRVL
QALQTGGLCPANWKPGQATL

Sequences:

>Translated_180_residues
MAERMVGKQAPRFEMEAVLANKEFGKVSLEENMKNDKWTVLFFYPMDFTFVCPTEITAMSDRYDEFEDLDAEVIGVSTDT
IHTHLAWINTDRKDNGLGQLKYPLAADTNHEVSREYGVLIEEEGVALRGLFIINPEGELQYQTVFHNNIGRDVDETLRVL
QALQTGGLCPANWKPGQATL
>Mature_179_residues
AERMVGKQAPRFEMEAVLANKEFGKVSLEENMKNDKWTVLFFYPMDFTFVCPTEITAMSDRYDEFEDLDAEVIGVSTDTI
HTHLAWINTDRKDNGLGQLKYPLAADTNHEVSREYGVLIEEEGVALRGLFIINPEGELQYQTVFHNNIGRDVDETLRVLQ
ALQTGGLCPANWKPGQATL

Specific function: Directly Reduces Organic Hydroperoxides In Its Reduced Dithiol Form. [C]

COG id: COG0450

COG function: function code O; Peroxiredoxin

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

Organism=Homo sapiens, GI32483377, Length=177, Percent_Identity=50.2824858757062, Blast_Score=181, Evalue=2e-46,
Organism=Homo sapiens, GI5802974, Length=179, Percent_Identity=49.7206703910614, Blast_Score=181, Evalue=2e-46,
Organism=Homo sapiens, GI4505591, Length=179, Percent_Identity=48.0446927374302, Blast_Score=181, Evalue=4e-46,
Organism=Homo sapiens, GI32455266, Length=179, Percent_Identity=48.0446927374302, Blast_Score=181, Evalue=4e-46,
Organism=Homo sapiens, GI32455264, Length=179, Percent_Identity=48.0446927374302, Blast_Score=181, Evalue=4e-46,
Organism=Homo sapiens, GI32189392, Length=179, Percent_Identity=51.9553072625698, Blast_Score=179, Evalue=1e-45,
Organism=Homo sapiens, GI5453549, Length=184, Percent_Identity=46.7391304347826, Blast_Score=174, Evalue=4e-44,
Organism=Homo sapiens, GI4758638, Length=189, Percent_Identity=30.6878306878307, Blast_Score=92, Evalue=2e-19,
Organism=Homo sapiens, GI33188454, Length=82, Percent_Identity=52.4390243902439, Blast_Score=87, Evalue=1e-17,
Organism=Escherichia coli, GI1786822, Length=168, Percent_Identity=38.6904761904762, Blast_Score=128, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI32565831, Length=184, Percent_Identity=51.6304347826087, Blast_Score=196, Evalue=4e-51,
Organism=Caenorhabditis elegans, GI193204376, Length=184, Percent_Identity=51.6304347826087, Blast_Score=196, Evalue=4e-51,
Organism=Caenorhabditis elegans, GI17554494, Length=180, Percent_Identity=48.8888888888889, Blast_Score=169, Evalue=9e-43,
Organism=Caenorhabditis elegans, GI25153706, Length=185, Percent_Identity=27.5675675675676, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323613, Length=179, Percent_Identity=50.8379888268156, Blast_Score=174, Evalue=6e-45,
Organism=Saccharomyces cerevisiae, GI6320661, Length=179, Percent_Identity=49.7206703910614, Blast_Score=173, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6319407, Length=184, Percent_Identity=28.804347826087, Blast_Score=96, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24656348, Length=180, Percent_Identity=52.7777777777778, Blast_Score=188, Evalue=1e-48,
Organism=Drosophila melanogaster, GI17864676, Length=180, Percent_Identity=52.7777777777778, Blast_Score=188, Evalue=1e-48,
Organism=Drosophila melanogaster, GI17738015, Length=179, Percent_Identity=49.1620111731844, Blast_Score=179, Evalue=1e-45,
Organism=Drosophila melanogaster, GI17157991, Length=177, Percent_Identity=52.5423728813559, Blast_Score=177, Evalue=2e-45,
Organism=Drosophila melanogaster, GI24641739, Length=177, Percent_Identity=52.5423728813559, Blast_Score=177, Evalue=2e-45,
Organism=Drosophila melanogaster, GI21357347, Length=181, Percent_Identity=44.7513812154696, Blast_Score=164, Evalue=4e-41,
Organism=Drosophila melanogaster, GI17975518, Length=189, Percent_Identity=31.7460317460317, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24652434, Length=189, Percent_Identity=31.7460317460317, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24652436, Length=189, Percent_Identity=31.7460317460317, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24581278, Length=181, Percent_Identity=30.939226519337, Blast_Score=97, Evalue=4e-21,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2250 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 6040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1580 Molecules/Cell In: Stationary-Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000866
- InterPro:   IPR019479
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF10417 1-cysPrx_C; PF00578 AhpC-TSA [H]

EC number: 1.6.4.- [C]

Molecular weight: Translated: 20403; Mature: 20272

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAERMVGKQAPRFEMEAVLANKEFGKVSLEENMKNDKWTVLFFYPMDFTFVCPTEITAMS
CCCCCCCCCCCCCEEEEEECCCCCCEEEHHHCCCCCCEEEEEEECCCEEEECCCCHHHHH
DRYDEFEDLDAEVIGVSTDTIHTHLAWINTDRKDNGLGQLKYPLAADTNHEVSREYGVLI
HCCCHHHCCCCEEEEECCCCEEEEEEEEECCCCCCCCCEEECCEECCCCCCCHHHHCEEE
EEEGVALRGLFIINPEGELQYQTVFHNNIGRDVDETLRVLQALQTGGLCPANWKPGQATL
CCCCEEEEEEEEECCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AERMVGKQAPRFEMEAVLANKEFGKVSLEENMKNDKWTVLFFYPMDFTFVCPTEITAMS
CCCCCCCCCCCCEEEEEECCCCCCEEEHHHCCCCCCEEEEEEECCCEEEECCCCHHHHH
DRYDEFEDLDAEVIGVSTDTIHTHLAWINTDRKDNGLGQLKYPLAADTNHEVSREYGVLI
HCCCHHHCCCCEEEEECCCCEEEEEEEEECCCCCCCCCEEECCEECCCCCCCHHHHCEEE
EEEGVALRGLFIINPEGELQYQTVFHNNIGRDVDETLRVLQALQTGGLCPANWKPGQATL
CCCCEEEEEEEEECCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ROOH; Proton; NADH [C]

Specific reaction: ROOH + Proton + NADH = an alcohol + H2O + NAD [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]