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The map label for this gene is ykfC [H]

Identifier: 52785167

GI number: 52785167

Start: 1398110

End: 1398997

Strand: Direct

Name: ykfC [H]

Synonym: BLi01399

Alternate gene names: 52785167

Gene position: 1398110-1398997 (Clockwise)

Preceding gene: 52785166

Following gene: 52785168

Centisome position: 33.11

GC content: 52.14

Gene sequence:

>888_bases
ATGAGCCTTTATACGACAGCCGTCTCAGTCGCCAATGTCTGGACCAGTCCGGAAGCGCCGCGCACAATTGACCGGATGAT
GCTGTCAAACCCTGTACATGTGAGAAGCTGGCTTGACGAATTGTCCTACGAGGAGCGCCTTGAGCTATGTACGGCAAACC
TTGTTCAAACGCAAGTGCTTTTCGGCGAAGATGTCCAACTTATAAAGGAGCGGGGAGACTGGGCGTTCGTCATCATTCCG
GGACAGCCGTCTGCAAAAGACAAACGCGGCTACCCGGGCTGGATTCCAAAGGACTGCCTGGTCGAAAAAAGCCCGGCGAA
GTCTGAGACAAGCGCCGTTATTCAAAAACCGACGGCTTTCTTATATGATGACAAGAAAACGAAAGTTATTGAGCTGAGCT
TTCTCACCAAGCTGCCCGTCTTGTCTTCCGGCGGCGTCTGGTTTGAGGTCGATACACCGCTTGGCCGCAAATGGCTGAAA
AAAGAAGATGCGGATCTCGAAACACCTGTAAAAGGCAGCGGAGACGATATCGTTCAAACCGGTCAGGCGTTTTTGAACCT
GCCCTATCTTTGGGGAGGGATGAGCGGGTTTGGCTACGACTGCTCAGGATTTGTCTTCAATATGCTGAAGGCGAACGGCC
GCTCCGCGCCGCGCGATGCCGGCGACCAGGCGAAAGGCGGAAAAGAGGTATCCTTCAGCAGCCCGAAGCGGGGCGATTTA
CTGTTTTTCGCATATGAAGAAGGCAGAGGACGGGTTCACCACGTCGGCATTTACTGCGGAAACGGAGAGATGCTTCACTC
TCCAAAAACGGGCAAATCGATCGAGGTCATATCTCTTAAAGGTACAATTTATGAAAAAGAGCTGTGTGCCATCAGGCGCT
ATTTTTAA

Upstream 100 bases:

>100_bases
TCGTTGAAGGCGGCATCCGCTATCATGCAGGAACGATCACGATACCGGATGCTCCCGGACTCGGAATCACGAAAGTCTGC
CATGAGGGGGGAGAGGACAA

Downstream 100 bases:

>100_bases
ATAAAACGGGGGGATTGGCATGCAAACCGGCGCTTTATTGAAAGTCAGCCGTCTGAAAATGCACTTTGACGCCGGAAGGG
GCAAAACGGTCAAAGCGGTC

Product: YkfC

Products: NA

Alternate protein names: Cell wall endopeptidase ykfC [H]

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MSLYTTAVSVANVWTSPEAPRTIDRMMLSNPVHVRSWLDELSYEERLELCTANLVQTQVLFGEDVQLIKERGDWAFVIIP
GQPSAKDKRGYPGWIPKDCLVEKSPAKSETSAVIQKPTAFLYDDKKTKVIELSFLTKLPVLSSGGVWFEVDTPLGRKWLK
KEDADLETPVKGSGDDIVQTGQAFLNLPYLWGGMSGFGYDCSGFVFNMLKANGRSAPRDAGDQAKGGKEVSFSSPKRGDL
LFFAYEEGRGRVHHVGIYCGNGEMLHSPKTGKSIEVISLKGTIYEKELCAIRRYF

Sequences:

>Translated_295_residues
MSLYTTAVSVANVWTSPEAPRTIDRMMLSNPVHVRSWLDELSYEERLELCTANLVQTQVLFGEDVQLIKERGDWAFVIIP
GQPSAKDKRGYPGWIPKDCLVEKSPAKSETSAVIQKPTAFLYDDKKTKVIELSFLTKLPVLSSGGVWFEVDTPLGRKWLK
KEDADLETPVKGSGDDIVQTGQAFLNLPYLWGGMSGFGYDCSGFVFNMLKANGRSAPRDAGDQAKGGKEVSFSSPKRGDL
LFFAYEEGRGRVHHVGIYCGNGEMLHSPKTGKSIEVISLKGTIYEKELCAIRRYF
>Mature_294_residues
SLYTTAVSVANVWTSPEAPRTIDRMMLSNPVHVRSWLDELSYEERLELCTANLVQTQVLFGEDVQLIKERGDWAFVIIPG
QPSAKDKRGYPGWIPKDCLVEKSPAKSETSAVIQKPTAFLYDDKKTKVIELSFLTKLPVLSSGGVWFEVDTPLGRKWLKK
EDADLETPVKGSGDDIVQTGQAFLNLPYLWGGMSGFGYDCSGFVFNMLKANGRSAPRDAGDQAKGGKEVSFSSPKRGDLL
FFAYEEGRGRVHHVGIYCGNGEMLHSPKTGKSIEVISLKGTIYEKELCAIRRYF

Specific function: Specifically hydrolyzes gamma-D-glutamyl-L-lysine bonds in murein peptides, releasing L-Ala-D-Glu [H]

COG id: COG0791

COG function: function code M; Cell wall-associated hydrolases (invasion-associated proteins)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nlpC/p60 family [H]

Homologues:

Organism=Escherichia coli, GI1787944, Length=207, Percent_Identity=28.5024154589372, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1786421, Length=116, Percent_Identity=34.4827586206897, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000064 [H]

Pfam domain/function: PF00877 NLPC_P60 [H]

EC number: NA

Molecular weight: Translated: 32802; Mature: 32671

Theoretical pI: Translated: 7.40; Mature: 7.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLYTTAVSVANVWTSPEAPRTIDRMMLSNPVHVRSWLDELSYEERLELCTANLVQTQVL
CCEEHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
FGEDVQLIKERGDWAFVIIPGQPSAKDKRGYPGWIPKDCLVEKSPAKSETSAVIQKPTAF
CCCCHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCHHHHCCCCCCCCHHHHHHHCCEEE
LYDDKKTKVIELSFLTKLPVLSSGGVWFEVDTPLGRKWLKKEDADLETPVKGSGDDIVQT
EECCCCCEEEEEEEHHCCCEEECCCEEEEECCCCCHHHHHCCCCCCCCCCCCCCCHHHHH
GQAFLNLPYLWGGMSGFGYDCSGFVFNMLKANGRSAPRDAGDQAKGGKEVSFSSPKRGDL
CHHHEECCHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCCCCCCCE
LFFAYEEGRGRVHHVGIYCGNGEMLHSPKTGKSIEVISLKGTIYEKELCAIRRYF
EEEEEECCCCEEEEEEEEECCCCEEECCCCCCEEEEEEECCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SLYTTAVSVANVWTSPEAPRTIDRMMLSNPVHVRSWLDELSYEERLELCTANLVQTQVL
CEEHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
FGEDVQLIKERGDWAFVIIPGQPSAKDKRGYPGWIPKDCLVEKSPAKSETSAVIQKPTAF
CCCCHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCHHHHCCCCCCCCHHHHHHHCCEEE
LYDDKKTKVIELSFLTKLPVLSSGGVWFEVDTPLGRKWLKKEDADLETPVKGSGDDIVQT
EECCCCCEEEEEEEHHCCCEEECCCEEEEECCCCCHHHHHCCCCCCCCCCCCCCCHHHHH
GQAFLNLPYLWGGMSGFGYDCSGFVFNMLKANGRSAPRDAGDQAKGGKEVSFSSPKRGDL
CHHHEECCHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCCCCCCCE
LFFAYEEGRGRVHHVGIYCGNGEMLHSPKTGKSIEVISLKGTIYEKELCAIRRYF
EEEEEECCCCEEEEEEEEECCCCEEECCCCCCEEEEEEECCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]