Click here to switch to the map view.

The map label for this gene is ykfB [H]

Identifier: 52785166

GI number: 52785166

Start: 1397016

End: 1398113

Strand: Direct

Name: ykfB [H]

Synonym: BLi01398

Alternate gene names: 52785166

Gene position: 1397016-1398113 (Clockwise)

Preceding gene: 52785165

Following gene: 52785167

Centisome position: 33.08

GC content: 54.01

Gene sequence:

>1098_bases
ATGAAGATTGCCGAGATTACGACAAACCGCATCGCCGTTCCGCTGAAAAAACCTTTTAAAACAGCGCTTCGAACCGTCCG
CACCGCAGAAGCCGTCATTGTCAAAGTAACGGCTGAAAACGGGATGACAGGCTGGGGAGAAGCGCCTCCGACTGCTGTTA
TTACCGGTGAGACGCTTGACAGTATTGAGGGGGCAGTCAACACGATTCTCGCACCCGCCCTCACCGGGTGCAACTTGCGA
ATGGCTGAGGCGATATTTCATGATATCGACAAGCTGATTGTTGGAAATACAAGCGCAAAGGCCGCTGTAGAAACAGCCGT
CTACGATTGCTTGGCGCAGCTTTGCGGTCTTCCCCTGTATCAATTGCTGGGCGGATACAGACGGGAGATCGAGACGGATT
TTACCGTCAGCGTCAATGACCCGGAAGAAATGGGGAAAGACGCGGCCCGCTATGTACAGGAAGGGTTTCGCACGCTCAAA
ATTAAGGTTGGAAAAAACGACATGGCAACAGACCTCGAACGGATCAGGGAAATCCGCAAACAAGCGGGTCCCGCTGTCCG
AATCCGCGTGGACGCCAATCAAGGCTGGACGGCGAAACAGGCGGTCAAAGCCATTCGGAAAATGGAGGATGAGGGGCTTT
CGATCGAGCTTGTCGAACAGCCGGTCCACCGGCTCGATCTCGAAGGCTTGAAACAGGTGACAGATGCCGTCGATACGCCG
ATTATGGCGGACGAAAGCGTGTTTACCCCAAGGGATGCTTTTCACGTGCTGAAAACGAGAAGCGCCGATCTCATCAATAT
TAAACTGATGAAGGCAGGCGGCATCCGCCAGGCGCTGAAAATCAACGCCATGGCAGAAGCGTGCGGAGTGGAATGCATGG
CCGGAAGCATGATCGAGACAAGGCTCGGCATCACAGCGGCCGTCCACTTCGCAGCAAGCCAGAAAAATGTGACAAAATTC
GATTTTGATGCGCCGCTCATGCTGAAGGAGGATATCGTTGAAGGCGGCATCCGCTATCATGCAGGAACGATCACGATACC
GGATGCTCCCGGACTCGGAATCACGAAAGTCTGCCATGAGGGGGGAGAGGACAAATGA

Upstream 100 bases:

>100_bases
CGAATATCGCCGTACCGGTAGGAGTGAAAGCCGTCCTTGACGCCGGCAAAAAACGGCTCACCATCGAACCGGGAATTTCT
TAATGGGAAAGGAGGCCGCC

Downstream 100 bases:

>100_bases
GCCTTTATACGACAGCCGTCTCAGTCGCCAATGTCTGGACCAGTCCGGAAGCGCCGCGCACAATTGACCGGATGATGCTG
TCAAACCCTGTACATGTGAG

Product: YkfB

Products: NA

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MKIAEITTNRIAVPLKKPFKTALRTVRTAEAVIVKVTAENGMTGWGEAPPTAVITGETLDSIEGAVNTILAPALTGCNLR
MAEAIFHDIDKLIVGNTSAKAAVETAVYDCLAQLCGLPLYQLLGGYRREIETDFTVSVNDPEEMGKDAARYVQEGFRTLK
IKVGKNDMATDLERIREIRKQAGPAVRIRVDANQGWTAKQAVKAIRKMEDEGLSIELVEQPVHRLDLEGLKQVTDAVDTP
IMADESVFTPRDAFHVLKTRSADLINIKLMKAGGIRQALKINAMAEACGVECMAGSMIETRLGITAAVHFAASQKNVTKF
DFDAPLMLKEDIVEGGIRYHAGTITIPDAPGLGITKVCHEGGEDK

Sequences:

>Translated_365_residues
MKIAEITTNRIAVPLKKPFKTALRTVRTAEAVIVKVTAENGMTGWGEAPPTAVITGETLDSIEGAVNTILAPALTGCNLR
MAEAIFHDIDKLIVGNTSAKAAVETAVYDCLAQLCGLPLYQLLGGYRREIETDFTVSVNDPEEMGKDAARYVQEGFRTLK
IKVGKNDMATDLERIREIRKQAGPAVRIRVDANQGWTAKQAVKAIRKMEDEGLSIELVEQPVHRLDLEGLKQVTDAVDTP
IMADESVFTPRDAFHVLKTRSADLINIKLMKAGGIRQALKINAMAEACGVECMAGSMIETRLGITAAVHFAASQKNVTKF
DFDAPLMLKEDIVEGGIRYHAGTITIPDAPGLGITKVCHEGGEDK
>Mature_365_residues
MKIAEITTNRIAVPLKKPFKTALRTVRTAEAVIVKVTAENGMTGWGEAPPTAVITGETLDSIEGAVNTILAPALTGCNLR
MAEAIFHDIDKLIVGNTSAKAAVETAVYDCLAQLCGLPLYQLLGGYRREIETDFTVSVNDPEEMGKDAARYVQEGFRTLK
IKVGKNDMATDLERIREIRKQAGPAVRIRVDANQGWTAKQAVKAIRKMEDEGLSIELVEQPVHRLDLEGLKQVTDAVDTP
IMADESVFTPRDAFHVLKTRSADLINIKLMKAGGIRQALKINAMAEACGVECMAGSMIETRLGITAAVHFAASQKNVTKF
DFDAPLMLKEDIVEGGIRYHAGTITIPDAPGLGITKVCHEGGEDK

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has probably a role in the metabolism of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the reverse reaction and the epimerization of the other Ala-X dipepti

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=336, Percent_Identity=29.1666666666667, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI48994953, Length=308, Percent_Identity=25.6493506493506, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 39555; Mature: 39555

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAEITTNRIAVPLKKPFKTALRTVRTAEAVIVKVTAENGMTGWGEAPPTAVITGETLD
CCCCEECCCEEEECCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCEEEECCHHH
SIEGAVNTILAPALTGCNLRMAEAIFHDIDKLIVGNTSAKAAVETAVYDCLAQLCGLPLY
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHH
QLLGGYRREIETDFTVSVNDPEEMGKDAARYVQEGFRTLKIKVGKNDMATDLERIREIRK
HHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHHH
QAGPAVRIRVDANQGWTAKQAVKAIRKMEDEGLSIELVEQPVHRLDLEGLKQVTDAVDTP
HCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHCHHHHHHHHHHHCCC
IMADESVFTPRDAFHVLKTRSADLINIKLMKAGGIRQALKINAMAEACGVECMAGSMIET
CCCCCCCCCCHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCHHHCCCHHHH
RLGITAAVHFAASQKNVTKFDFDAPLMLKEDIVEGGIRYHAGTITIPDAPGLGITKVCHE
HHHHHHHEEEHHCCCCCEEECCCCCHHHHHHHHHCCCEEECCEEECCCCCCCCHHHHHHC
GGEDK
CCCCC
>Mature Secondary Structure
MKIAEITTNRIAVPLKKPFKTALRTVRTAEAVIVKVTAENGMTGWGEAPPTAVITGETLD
CCCCEECCCEEEECCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCEEEECCHHH
SIEGAVNTILAPALTGCNLRMAEAIFHDIDKLIVGNTSAKAAVETAVYDCLAQLCGLPLY
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHH
QLLGGYRREIETDFTVSVNDPEEMGKDAARYVQEGFRTLKIKVGKNDMATDLERIREIRK
HHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHHH
QAGPAVRIRVDANQGWTAKQAVKAIRKMEDEGLSIELVEQPVHRLDLEGLKQVTDAVDTP
HCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHCHHHHHHHHHHHCCC
IMADESVFTPRDAFHVLKTRSADLINIKLMKAGGIRQALKINAMAEACGVECMAGSMIET
CCCCCCCCCCHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCHHHCCCHHHH
RLGITAAVHFAASQKNVTKFDFDAPLMLKEDIVEGGIRYHAGTITIPDAPGLGITKVCHE
HHHHHHHEEEHHCCCCCEEECCCCCHHHHHHHHHCCCEEECCEEECCCCCCCCHHHHHHC
GGEDK
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]