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The map label for this gene is ykfA [H]

Identifier: 52785165

GI number: 52785165

Start: 1396075

End: 1396998

Strand: Direct

Name: ykfA [H]

Synonym: BLi01397

Alternate gene names: 52785165

Gene position: 1396075-1396998 (Clockwise)

Preceding gene: 52785164

Following gene: 52785166

Centisome position: 33.06

GC content: 50.54

Gene sequence:

>924_bases
TTGAATATTGCGCCTCAAAGATTAATGAAGGGAGATACGGTTGGTGTGATTGCGCCGGCCAGTCCGCCCGTTCCTGAAAA
GCTTGACCGGGCCGTCCGGTTTTTAAAAGAGCTTGGACTTGAAGTCAAAGTGGGACGCTCAGCCGCCGAAAGACACGGCT
ATTTGGCGGGGAGCGATGAAGAGCGTCTGGGGGATCTGCACGACATGTTTGAAGACCGTTCGGTTAAGGCTGTCATCTGT
GCCTGCGGAGGTTACGGAACGGCACGGATTGCCGGGAAAATTGACTACCGGCTCATCAAAAGCCGCCCGAAAATTTTTTG
GGGCTATTCAGATATCACTTTTTTACATACAGCGATCAGGCAAAATACCGGACTTATTACATTTCACGGACCGATGCTCA
GTTCTGACATCGGCAAAGATGACGTGCATCCGCTTACCCGCCGCTCCTTTCAGCAGCTGTTTCAAAAAATGACGCTCTCT
TATACAGAGGAACTGTCGCCTTTGGAAACGCTCGTACACGGCGCGGCGGAAGGGGAGCTCGTCGGCGGAAACCTCGCTCT
TCTGGTGACGACGCTAGGTACGCCTTATGAAATTGATACGAAAGGAAAGCTTTTGTTTATCGAGGATGTCGATGAAGAGC
CTTATCGAATCGACCGGATGCTGAATCAATTAAAAATGGCCGGGAAATTATCGGACGCCGCCGGCATCCTGCTGTGCGAT
TTTCATAACTGCATCCCGAAAAAACGCGATAAGTCGCTCACATTAAAAGAGGTTTTCCAAGATTATATCGCCTCTGAAGG
AAAACCGGCGCTGTCCGGCTTTAAAATCGGCCATTGTTCACCGAATATCGCCGTACCGGTAGGAGTGAAAGCCGTCCTTG
ACGCCGGCAAAAAACGGCTCACCATCGAACCGGGAATTTCTTAA

Upstream 100 bases:

>100_bases
GCCGAAAAAACGTCATGACCAAAATTGTCCGTCTGCTATTTTCTTTGTGCATACTGCCGCACAGGAGCGGACGGACTTTT
CATAAAGGGGGATGTCTCTG

Downstream 100 bases:

>100_bases
TGGGAAAGGAGGCCGCCATGAAGATTGCCGAGATTACGACAAACCGCATCGCCGTTCCGCTGAAAAAACCTTTTAAAACA
GCGCTTCGAACCGTCCGCAC

Product: YkfA

Products: NA

Alternate protein names: LD-carboxypeptidase [H]

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MNIAPQRLMKGDTVGVIAPASPPVPEKLDRAVRFLKELGLEVKVGRSAAERHGYLAGSDEERLGDLHDMFEDRSVKAVIC
ACGGYGTARIAGKIDYRLIKSRPKIFWGYSDITFLHTAIRQNTGLITFHGPMLSSDIGKDDVHPLTRRSFQQLFQKMTLS
YTEELSPLETLVHGAAEGELVGGNLALLVTTLGTPYEIDTKGKLLFIEDVDEEPYRIDRMLNQLKMAGKLSDAAGILLCD
FHNCIPKKRDKSLTLKEVFQDYIASEGKPALSGFKIGHCSPNIAVPVGVKAVLDAGKKRLTIEPGIS

Sequences:

>Translated_307_residues
MNIAPQRLMKGDTVGVIAPASPPVPEKLDRAVRFLKELGLEVKVGRSAAERHGYLAGSDEERLGDLHDMFEDRSVKAVIC
ACGGYGTARIAGKIDYRLIKSRPKIFWGYSDITFLHTAIRQNTGLITFHGPMLSSDIGKDDVHPLTRRSFQQLFQKMTLS
YTEELSPLETLVHGAAEGELVGGNLALLVTTLGTPYEIDTKGKLLFIEDVDEEPYRIDRMLNQLKMAGKLSDAAGILLCD
FHNCIPKKRDKSLTLKEVFQDYIASEGKPALSGFKIGHCSPNIAVPVGVKAVLDAGKKRLTIEPGIS
>Mature_307_residues
MNIAPQRLMKGDTVGVIAPASPPVPEKLDRAVRFLKELGLEVKVGRSAAERHGYLAGSDEERLGDLHDMFEDRSVKAVIC
ACGGYGTARIAGKIDYRLIKSRPKIFWGYSDITFLHTAIRQNTGLITFHGPMLSSDIGKDDVHPLTRRSFQQLFQKMTLS
YTEELSPLETLVHGAAEGELVGGNLALLVTTLGTPYEIDTKGKLLFIEDVDEEPYRIDRMLNQLKMAGKLSDAAGILLCD
FHNCIPKKRDKSLTLKEVFQDYIASEGKPALSGFKIGHCSPNIAVPVGVKAVLDAGKKRLTIEPGIS

Specific function: May be involved in the degradation of peptidoglycan by catalyzing the cleavage of the terminal D-alanine residue from cytoplasmic murein peptides [H]

COG id: COG1619

COG function: function code V; Uncharacterized proteins, homologs of microcin C7 resistance protein MccF

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S66 family [H]

Homologues:

Organism=Escherichia coli, GI1787441, Length=298, Percent_Identity=30.2013422818792, Blast_Score=140, Evalue=8e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003507 [H]

Pfam domain/function: PF02016 Peptidase_S66 [H]

EC number: NA

Molecular weight: Translated: 33662; Mature: 33662

Theoretical pI: Translated: 7.92; Mature: 7.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIAPQRLMKGDTVGVIAPASPPVPEKLDRAVRFLKELGLEVKVGRSAAERHGYLAGSDE
CCCCHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCEEEECCHHHHHCCCCCCCCH
ERLGDLHDMFEDRSVKAVICACGGYGTARIAGKIDYRLIKSRPKIFWGYSDITFLHTAIR
HHHHHHHHHHHCCCCEEEEEECCCCCCEEEECHHHHHHHHCCCCEEECCCHHHHHHHHHH
QNTGLITFHGPMLSSDIGKDDVHPLTRRSFQQLFQKMTLSYTEELSPLETLVHGAAEGEL
CCCCEEEEECCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCEE
VGGNLALLVTTLGTPYEIDTKGKLLFIEDVDEEPYRIDRMLNQLKMAGKLSDAAGILLCD
ECCCEEEEEEECCCCEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEE
FHNCIPKKRDKSLTLKEVFQDYIASEGKPALSGFKIGHCSPNIAVPVGVKAVLDAGKKRL
CHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCEECCCHHHHHHCCCCEE
TIEPGIS
EECCCCC
>Mature Secondary Structure
MNIAPQRLMKGDTVGVIAPASPPVPEKLDRAVRFLKELGLEVKVGRSAAERHGYLAGSDE
CCCCHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCEEEECCHHHHHCCCCCCCCH
ERLGDLHDMFEDRSVKAVICACGGYGTARIAGKIDYRLIKSRPKIFWGYSDITFLHTAIR
HHHHHHHHHHHCCCCEEEEEECCCCCCEEEECHHHHHHHHCCCCEEECCCHHHHHHHHHH
QNTGLITFHGPMLSSDIGKDDVHPLTRRSFQQLFQKMTLSYTEELSPLETLVHGAAEGEL
CCCCEEEEECCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCEE
VGGNLALLVTTLGTPYEIDTKGKLLFIEDVDEEPYRIDRMLNQLKMAGKLSDAAGILLCD
ECCCEEEEEEECCCCEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEE
FHNCIPKKRDKSLTLKEVFQDYIASEGKPALSGFKIGHCSPNIAVPVGVKAVLDAGKKRL
CHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCEECCCHHHHHHCCCCEE
TIEPGIS
EECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]