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The map label for this gene is fabI [H]

Identifier: 52785037

GI number: 52785037

Start: 1276753

End: 1277529

Strand: Direct

Name: fabI [H]

Synonym: BLi01266

Alternate gene names: 52785037

Gene position: 1276753-1277529 (Clockwise)

Preceding gene: 52785036

Following gene: 52785038

Centisome position: 30.24

GC content: 52.25

Gene sequence:

>777_bases
ATGAATTTATCACTAGAAGGCCGGAATATCGTCGTCATGGGTGTAGCAAATAAACGAAGCATCGCATGGGGAATCGCCCG
CTCGCTTCACAATGCAGGCGCAAGGCTGATTTTTACGTATGCGGGCGAGCGGCTTGAAAAATCGGTCAAGGAGCTGGCTG
ATTCTTTGGAGCGAAACGATTCAATCGTACTTCCTTGTGATGTCACTAATGACCAGGAAATTGAAGCATGCTTCGCCGAC
ATCAAAAAAGAAGTCGGGGTCATCCACGGCATTGCACACTGCATCGCTTTCGCCAACAAAGAAGACTTGGCTGGCGAATA
CTTAAATACTACCCGCGACGGTTTTCTGCTCGCACACAATATCAGCTCCTACTCTCTGACAGCGGTCGCTAAAGCGGCGC
GCGGCATCATGACTGAAGGCGGAAGCATCGTCACGCTGACATACCTTGGCGGCGAGCGCGTTGTATCCAACTACAATGTC
ATGGGTGTTGCGAAAGCATCGCTTGATGCCAGCGTCAAATACTTGGCGAGCGATCTCGGAAAAGACGGAATCCGCGTCAA
CAGCATTTCCGCAGGGCCGATCCGGACATTGTCCGCAAAAGGAATCAGCGACTTCAACTCGATTTTAAAAGAAATCGAAG
AGCGCGCGCCGCTTCGCCGCACAACGACTCCGGAAGAAGTCGGCGACACGGCTGCTTTCCTGTTCAGCGACCTTTCCCGC
GGCATGACCGGCGAAAACCTGCATGTTGATTCAGGCTACCATATCATCGCGCGATAA

Upstream 100 bases:

>100_bases
CTTTTTATATTCTGAAATTGTCAGCCTGCTATTAATTGTGATACTATATATTATGATCAGGTACTAATAAAATGTTAATC
ATTAGGAGGATTTCATACAT

Downstream 100 bases:

>100_bases
AATAGCGCTAAAAAAGCAATCCGCTAAAGGATTGCTTTTTCTTTTCTTCTGATTTCTCCTTTTTTGCATATAGATATACC
ATGAACAGATCGGAGGGATC

Product: enoyl-(acyl carrier protein) reductase

Products: NA

Alternate protein names: Cold shock-induced protein 15; CSI15; NADH-dependent enoyl-ACP reductase; Vegetative protein 241; VEG241 [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MNLSLEGRNIVVMGVANKRSIAWGIARSLHNAGARLIFTYAGERLEKSVKELADSLERNDSIVLPCDVTNDQEIEACFAD
IKKEVGVIHGIAHCIAFANKEDLAGEYLNTTRDGFLLAHNISSYSLTAVAKAARGIMTEGGSIVTLTYLGGERVVSNYNV
MGVAKASLDASVKYLASDLGKDGIRVNSISAGPIRTLSAKGISDFNSILKEIEERAPLRRTTTPEEVGDTAAFLFSDLSR
GMTGENLHVDSGYHIIAR

Sequences:

>Translated_258_residues
MNLSLEGRNIVVMGVANKRSIAWGIARSLHNAGARLIFTYAGERLEKSVKELADSLERNDSIVLPCDVTNDQEIEACFAD
IKKEVGVIHGIAHCIAFANKEDLAGEYLNTTRDGFLLAHNISSYSLTAVAKAARGIMTEGGSIVTLTYLGGERVVSNYNV
MGVAKASLDASVKYLASDLGKDGIRVNSISAGPIRTLSAKGISDFNSILKEIEERAPLRRTTTPEEVGDTAAFLFSDLSR
GMTGENLHVDSGYHIIAR
>Mature_258_residues
MNLSLEGRNIVVMGVANKRSIAWGIARSLHNAGARLIFTYAGERLEKSVKELADSLERNDSIVLPCDVTNDQEIEACFAD
IKKEVGVIHGIAHCIAFANKEDLAGEYLNTTRDGFLLAHNISSYSLTAVAKAARGIMTEGGSIVTLTYLGGERVVSNYNV
MGVAKASLDASVKYLASDLGKDGIRVNSISAGPIRTLSAKGISDFNSILKEIEERAPLRRTTTPEEVGDTAAFLFSDLSR
GMTGENLHVDSGYHIIAR

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Inner Membrane-Associated [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Homo sapiens, GI7705925, Length=258, Percent_Identity=27.906976744186, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1787545, Length=254, Percent_Identity=51.5748031496063, Blast_Score=261, Evalue=5e-71,
Organism=Escherichia coli, GI87082100, Length=257, Percent_Identity=29.1828793774319, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1790717, Length=251, Percent_Identity=23.9043824701195, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1787335, Length=262, Percent_Identity=27.8625954198473, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 27762; Mature: 27762

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLSLEGRNIVVMGVANKRSIAWGIARSLHNAGARLIFTYAGERLEKSVKELADSLERND
CCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHCCC
SIVLPCDVTNDQEIEACFADIKKEVGVIHGIAHCIAFANKEDLAGEYLNTTRDGFLLAHN
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCHHHHCCCCCCEEEEEC
ISSYSLTAVAKAARGIMTEGGSIVTLTYLGGERVVSNYNVMGVAKASLDASVKYLASDLG
CCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHCCCCEEEEEHHHHHHHHHHHHHHCC
KDGIRVNSISAGPIRTLSAKGISDFNSILKEIEERAPLRRTTTPEEVGDTAAFLFSDLSR
CCCEEEEEECCCCCEEECCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHC
GMTGENLHVDSGYHIIAR
CCCCCEEEECCCCEEEEC
>Mature Secondary Structure
MNLSLEGRNIVVMGVANKRSIAWGIARSLHNAGARLIFTYAGERLEKSVKELADSLERND
CCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHCCC
SIVLPCDVTNDQEIEACFADIKKEVGVIHGIAHCIAFANKEDLAGEYLNTTRDGFLLAHN
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCHHHHCCCCCCEEEEEC
ISSYSLTAVAKAARGIMTEGGSIVTLTYLGGERVVSNYNVMGVAKASLDASVKYLASDLG
CCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHCCCCEEEEEHHHHHHHHHHHHHHCC
KDGIRVNSISAGPIRTLSAKGISDFNSILKEIEERAPLRRTTTPEEVGDTAAFLFSDLSR
CCCEEEEEECCCCCEEECCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHC
GMTGENLHVDSGYHIIAR
CCCCCEEEECCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377; 8755892; 9298659 [H]