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The map label for this gene is carA [H]

Identifier: 52784982

GI number: 52784982

Start: 1220866

End: 1221930

Strand: Direct

Name: carA [H]

Synonym: BLi01210

Alternate gene names: 52784982

Gene position: 1220866-1221930 (Clockwise)

Preceding gene: 52784981

Following gene: 52784983

Centisome position: 28.91

GC content: 51.36

Gene sequence:

>1065_bases
ATGGAAGGATATTTAGTTCTTGAAGATGGTACATCCTTCAGCGGAGAACTGGACGGTCTTGACGGCTGCACCGGTGAAGT
CGTCTTTTTTACGGGCATGACCGGTTATCAGGAGGTTTTGACAGATCCTTCATATAAAGGCCAGATTATTGTCTTTACGT
ATCCGCTGATTGGCAACTATGGAATTAATGGAAGCGATGCTGAAAGCAAACAGCCTCAGGTGAGAGGGGTCGTCGTCTAT
GAAGCGTGCGACAGATTTTCCCACCATCAGGCGACAGAAAGCCTTCGGGAATACTTGGCGAAGTGGGATGTTCCGCTTTT
GTCGCACGTGGACACAAGAGCGGTTGTTCAGCATATCAGGGCAAAAGGGACGCTGAATGCGAGGCTGACGAAACAAAAGG
ACGACATCCCGGCTCCGCGCCAGCTGGAAAACTATGCGGCACAGGTTTCCGGACAAAACGAAAACGGCGCTTTTGGGGAA
GGCAATACGCATATTGCGCTGATCGATTTCGGCTATAAAAAGTCGATCGCTTCATCGCTAGCAAACCGGGGCTGCAAAGT
GACGGTCGTTCCCTATCAAGAGATGGACGGCATTGCGAAGCTGAATCCGGACGGAATCGTCCTTTCAAACGGGCCGGGCG
ATCCGAAAGCGATCCTTCCATATATGGATGCACTCCGGGGTATCATTTCTTCCTATCCGACGCTCGGCATCTGCCTCGGG
CACCAGCTGATCGCGCTCGCATTCGGAAGCGACACGTATAAGCTTCCGTTCGGCCACAGGGGAGCCAATCATCCGGTCAT
GGACAAAGAGACCGGACGCGTGTTCATGACAAGCCAAAATCACAGCTATGTCGTCGATCGGGACACCATCAGCGAAAATG
AATTTTCCGTCAGGTTTCATCATGTCAACGACGGTTCAGTAGAAGGGCTGATGCATAAAACACTGCCTGTCCTATCCGTT
CAGTTTCATCCGGAAGCCCATCCGGGACCGGCTGAAAGCGAATGGATATTCGGAGAATATTTGCAGAAATTGAACGAAGC
AAGGAGAGAGATCGCCCATGCCTAA

Upstream 100 bases:

>100_bases
GTCATGGCAAATCATTCTGCTGTAAAGCAGTAATTTTTTTTGAAATGAAAGTATAAAAATTCATGAATATCACTATTTAT
TCATCAAAGGTGGGCATAAG

Downstream 100 bases:

>100_bases
AGACCAAACCATCAAAAGCATTCTCGTTATCGGATCGGGGCCGATTATCATCGGGCAGGCCGCTGAATTCGACTACTCAG
GAACCCAAGGCTGCATGGCG

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MEGYLVLEDGTSFSGELDGLDGCTGEVVFFTGMTGYQEVLTDPSYKGQIIVFTYPLIGNYGINGSDAESKQPQVRGVVVY
EACDRFSHHQATESLREYLAKWDVPLLSHVDTRAVVQHIRAKGTLNARLTKQKDDIPAPRQLENYAAQVSGQNENGAFGE
GNTHIALIDFGYKKSIASSLANRGCKVTVVPYQEMDGIAKLNPDGIVLSNGPGDPKAILPYMDALRGIISSYPTLGICLG
HQLIALAFGSDTYKLPFGHRGANHPVMDKETGRVFMTSQNHSYVVDRDTISENEFSVRFHHVNDGSVEGLMHKTLPVLSV
QFHPEAHPGPAESEWIFGEYLQKLNEARREIAHA

Sequences:

>Translated_354_residues
MEGYLVLEDGTSFSGELDGLDGCTGEVVFFTGMTGYQEVLTDPSYKGQIIVFTYPLIGNYGINGSDAESKQPQVRGVVVY
EACDRFSHHQATESLREYLAKWDVPLLSHVDTRAVVQHIRAKGTLNARLTKQKDDIPAPRQLENYAAQVSGQNENGAFGE
GNTHIALIDFGYKKSIASSLANRGCKVTVVPYQEMDGIAKLNPDGIVLSNGPGDPKAILPYMDALRGIISSYPTLGICLG
HQLIALAFGSDTYKLPFGHRGANHPVMDKETGRVFMTSQNHSYVVDRDTISENEFSVRFHHVNDGSVEGLMHKTLPVLSV
QFHPEAHPGPAESEWIFGEYLQKLNEARREIAHA
>Mature_354_residues
MEGYLVLEDGTSFSGELDGLDGCTGEVVFFTGMTGYQEVLTDPSYKGQIIVFTYPLIGNYGINGSDAESKQPQVRGVVVY
EACDRFSHHQATESLREYLAKWDVPLLSHVDTRAVVQHIRAKGTLNARLTKQKDDIPAPRQLENYAAQVSGQNENGAFGE
GNTHIALIDFGYKKSIASSLANRGCKVTVVPYQEMDGIAKLNPDGIVLSNGPGDPKAILPYMDALRGIISSYPTLGICLG
HQLIALAFGSDTYKLPFGHRGANHPVMDKETGRVFMTSQNHSYVVDRDTISENEFSVRFHHVNDGSVEGLMHKTLPVLSV
QFHPEAHPGPAESEWIFGEYLQKLNEARREIAHA

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=360, Percent_Identity=40.8333333333333, Blast_Score=246, Evalue=2e-65,
Organism=Homo sapiens, GI21361331, Length=363, Percent_Identity=35.5371900826446, Blast_Score=213, Evalue=1e-55,
Organism=Homo sapiens, GI169790915, Length=363, Percent_Identity=35.5371900826446, Blast_Score=213, Evalue=1e-55,
Organism=Escherichia coli, GI1786215, Length=374, Percent_Identity=38.7700534759358, Blast_Score=249, Evalue=2e-67,
Organism=Caenorhabditis elegans, GI193204318, Length=368, Percent_Identity=39.945652173913, Blast_Score=254, Evalue=6e-68,
Organism=Saccharomyces cerevisiae, GI6324878, Length=363, Percent_Identity=38.5674931129477, Blast_Score=245, Evalue=9e-66,
Organism=Saccharomyces cerevisiae, GI6322331, Length=392, Percent_Identity=36.2244897959184, Blast_Score=225, Evalue=6e-60,
Organism=Drosophila melanogaster, GI45555749, Length=377, Percent_Identity=37.4005305039788, Blast_Score=232, Evalue=3e-61,
Organism=Drosophila melanogaster, GI24642586, Length=377, Percent_Identity=37.4005305039788, Blast_Score=232, Evalue=3e-61,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 38910; Mature: 38910

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGYLVLEDGTSFSGELDGLDGCTGEVVFFTGMTGYQEVLTDPSYKGQIIVFTYPLIGNY
CCCEEEEECCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHCCCCCCCEEEEEEECCCCCC
GINGSDAESKQPQVRGVVVYEACDRFSHHQATESLREYLAKWDVPLLSHVDTRAVVQHIR
CCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHH
AKGTLNARLTKQKDDIPAPRQLENYAAQVSGQNENGAFGEGNTHIALIDFGYKKSIASSL
HCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCHHHHHHHHH
ANRGCKVTVVPYQEMDGIAKLNPDGIVLSNGPGDPKAILPYMDALRGIISSYPTLGICLG
HHCCCEEEEECHHHCCCCEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHH
HQLIALAFGSDTYKLPFGHRGANHPVMDKETGRVFMTSQNHSYVVDRDTISENEFSVRFH
CEEEEEEECCCEEECCCCCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCCCCCEEEEEE
HVNDGSVEGLMHKTLPVLSVQFHPEAHPGPAESEWIFGEYLQKLNEARREIAHA
ECCCCCHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEGYLVLEDGTSFSGELDGLDGCTGEVVFFTGMTGYQEVLTDPSYKGQIIVFTYPLIGNY
CCCEEEEECCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHCCCCCCCEEEEEEECCCCCC
GINGSDAESKQPQVRGVVVYEACDRFSHHQATESLREYLAKWDVPLLSHVDTRAVVQHIR
CCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHH
AKGTLNARLTKQKDDIPAPRQLENYAAQVSGQNENGAFGEGNTHIALIDFGYKKSIASSL
HCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCHHHHHHHHH
ANRGCKVTVVPYQEMDGIAKLNPDGIVLSNGPGDPKAILPYMDALRGIISSYPTLGICLG
HHCCCEEEEECHHHCCCCEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHH
HQLIALAFGSDTYKLPFGHRGANHPVMDKETGRVFMTSQNHSYVVDRDTISENEFSVRFH
CEEEEEEECCCEEECCCCCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCCCCCEEEEEE
HVNDGSVEGLMHKTLPVLSVQFHPEAHPGPAESEWIFGEYLQKLNEARREIAHA
ECCCCCHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8025667; 9384377 [H]