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The map label for this gene is yitJ [H]

Identifier: 52784965

GI number: 52784965

Start: 1205712

End: 1207541

Strand: Reverse

Name: yitJ [H]

Synonym: BLi01193

Alternate gene names: 52784965

Gene position: 1207541-1205712 (Counterclockwise)

Preceding gene: 52784966

Following gene: 52784964

Centisome position: 28.6

GC content: 51.97

Gene sequence:

>1830_bases
ATGGGTTTTTTGGAGGATATAAAAAACAAAACGTTAATCGCTGACGGCGCAATGGGCACGCTTCTGTATTCCTATGGGAT
CGACAGGTGTTTTGAGGAACTGAACATCTCCAAGCCCGAAGAAATAAGACGCGTCCACGAAGCGTATGTACAGGCCGGCG
CCGACATTATTCAAACGAACACGTACGGCGCAAACTTCATCAAGCTGTCCAGATACGGACTCGAAGACGAAACAAAACAA
ATCAACGAAAAAGCCGTCCGCCTCGCCCGCGCCGCCGCCGGCTCCGCTTATGTTCTCGGTACGCTCGGCGGAATCCGGAC
GTTCAACAAAAACTCGTATTCTCTCGAGGACATTAAAAGAAGCTTCCGCGAACAGCTCTACCTTCTGTTAAATGAGCAGC
CGGACGGACTTCTGCTCGAAACCTATTACGACTTAGAAGAAGCGCGCGAAGTTTTAAAGATTGCCAGAAAGGAAACGGAG
CTGCCGATCATTTTGAATGTCTCGATGCACGAAGAGGGCGTTCTTCAGGACGGCACCCCGCTTGCGGACGGTCTGAAGCA
GCTGGCATCACTCGGCGCAGATGTCGTCGGCATCAACTGCCGTCTCGGCCCTTATCATATGATCAGAGCGCTTGAAGAAG
TCCCGCTTTTAGAAGATGCCTATCTTTCCGTCTATCCGAACAGCAGCCTCCCGTCCCTTGTCGAAGGCAGGCTTGTTTAT
GAGACGGATGATGAATATTTCAGGGAAAGCGCGGAGGAATTCCGCAATCAAGGCGCACGGATCATCGGCGGCTGCTGCGG
AACGACGCCGAACCACATCAGAGCGATGGCTGAGGCCGTCAAAGGACTTCCTCCAGTCACCGAAAAACGAGTGAAAATCA
GAAAGAAAGCGGCGCTTGCCGTTCAAAACGAAAGGACAGAGCCTGCTCTTGACGATCTGGCAAAGCAGAAACGGTCCATT
ATCGTCGAGCTCGATCCTCCAAAACAATTAAACTTTGAGAAATTTTTGCACGCCGCAGAGGAGCTAAAATCAGCCGGAAT
CGATGCACTGACATTGGCGGACAACTCCCTTGCCACACCGAGGATCAGCAATGTCGCCTGCGGGGCGCTCTTAAAACAGC
GCCTCGATATGAGGTCGCTGATTCATATCACATGCAGAGACCGCAACCTGATCGGCCTGCAGTCCCATTTAATGGGACTC
GATACGCTGGGGCTGTCAGACGTGCTGGCGATTACCGGCGATCCGTCGAAAATCGGCGACTTTCCGGGAGCGACTTCCGT
CTATGACCTGACATCGTTCGATTTGATCAGCCTGATCAAGCAGTTTAACGAAGGCTTGTCCTATTCGGGTAAACCGCTCG
GCAAGAAAACGAATTTCTCAGTGGCGGCCGCCTTCAATCCGAATGTCAGACACATTGACAAAGCGGTGAAACGGCTTGAG
AAAAAAATCGCCTGCGGCGCAGACTATTTCATTTCCCAGCCTGTCTATTCCGAGGAGCAGCTCATTAAAATCCACAAGGA
AACGCGGCATCTTGAAACACCGATCTATATCGGCGTCATGCCGCTCACCAGCAGCCGCAACGCGGAATTCATCCACAACG
AAATCCCCGGCATCAAGCTGTCTGATTCAATCCGCGAAAAGATGGCGCTGGCAGGAAACGACAAACAAAAACAGGCCGAG
GAAGGGCTCGCGATCGCTCGCTCTCTGCTTGATGCGGCGTGCGACCTGTTCAACGGCATTTATCTGATCACACCGTTTTT
GCGGTCCGATCTAACCGCCGAGCTGACGGCATATATCCATCAGAAAGAAAAGGAGAAGACAAATGTCTAA

Upstream 100 bases:

>100_bases
GCCTGCTTGGAAGATAAGAAGACGGACAAATGTCGCTGAAAAGTCTTCTGCTTCCCAGAAGACTTTTTTGTTTTATTAAG
AATGATTAAGGGGGAAAACG

Downstream 100 bases:

>100_bases
CATCAATGACCAGCTGAAGAAAAAAATCCTCGTCCTTGACGGTGCGATGGGAACGATGATCCAAGACGCCGGTTTGTCCG
CCGCAGACTTCGGAGGCGAG

Product: bifunctional homocysteine S-methyltransferase/5,10-methylenetetrahydrofolate reductase protein

Products: NA

Alternate protein names: Homocysteine S-methyltransferase; S-methylmethionine:homocysteine methyltransferase; 5,10-methylenetetrahydrofolate reductase [H]

Number of amino acids: Translated: 609; Mature: 608

Protein sequence:

>609_residues
MGFLEDIKNKTLIADGAMGTLLYSYGIDRCFEELNISKPEEIRRVHEAYVQAGADIIQTNTYGANFIKLSRYGLEDETKQ
INEKAVRLARAAAGSAYVLGTLGGIRTFNKNSYSLEDIKRSFREQLYLLLNEQPDGLLLETYYDLEEAREVLKIARKETE
LPIILNVSMHEEGVLQDGTPLADGLKQLASLGADVVGINCRLGPYHMIRALEEVPLLEDAYLSVYPNSSLPSLVEGRLVY
ETDDEYFRESAEEFRNQGARIIGGCCGTTPNHIRAMAEAVKGLPPVTEKRVKIRKKAALAVQNERTEPALDDLAKQKRSI
IVELDPPKQLNFEKFLHAAEELKSAGIDALTLADNSLATPRISNVACGALLKQRLDMRSLIHITCRDRNLIGLQSHLMGL
DTLGLSDVLAITGDPSKIGDFPGATSVYDLTSFDLISLIKQFNEGLSYSGKPLGKKTNFSVAAAFNPNVRHIDKAVKRLE
KKIACGADYFISQPVYSEEQLIKIHKETRHLETPIYIGVMPLTSSRNAEFIHNEIPGIKLSDSIREKMALAGNDKQKQAE
EGLAIARSLLDAACDLFNGIYLITPFLRSDLTAELTAYIHQKEKEKTNV

Sequences:

>Translated_609_residues
MGFLEDIKNKTLIADGAMGTLLYSYGIDRCFEELNISKPEEIRRVHEAYVQAGADIIQTNTYGANFIKLSRYGLEDETKQ
INEKAVRLARAAAGSAYVLGTLGGIRTFNKNSYSLEDIKRSFREQLYLLLNEQPDGLLLETYYDLEEAREVLKIARKETE
LPIILNVSMHEEGVLQDGTPLADGLKQLASLGADVVGINCRLGPYHMIRALEEVPLLEDAYLSVYPNSSLPSLVEGRLVY
ETDDEYFRESAEEFRNQGARIIGGCCGTTPNHIRAMAEAVKGLPPVTEKRVKIRKKAALAVQNERTEPALDDLAKQKRSI
IVELDPPKQLNFEKFLHAAEELKSAGIDALTLADNSLATPRISNVACGALLKQRLDMRSLIHITCRDRNLIGLQSHLMGL
DTLGLSDVLAITGDPSKIGDFPGATSVYDLTSFDLISLIKQFNEGLSYSGKPLGKKTNFSVAAAFNPNVRHIDKAVKRLE
KKIACGADYFISQPVYSEEQLIKIHKETRHLETPIYIGVMPLTSSRNAEFIHNEIPGIKLSDSIREKMALAGNDKQKQAE
EGLAIARSLLDAACDLFNGIYLITPFLRSDLTAELTAYIHQKEKEKTNV
>Mature_608_residues
GFLEDIKNKTLIADGAMGTLLYSYGIDRCFEELNISKPEEIRRVHEAYVQAGADIIQTNTYGANFIKLSRYGLEDETKQI
NEKAVRLARAAAGSAYVLGTLGGIRTFNKNSYSLEDIKRSFREQLYLLLNEQPDGLLLETYYDLEEAREVLKIARKETEL
PIILNVSMHEEGVLQDGTPLADGLKQLASLGADVVGINCRLGPYHMIRALEEVPLLEDAYLSVYPNSSLPSLVEGRLVYE
TDDEYFRESAEEFRNQGARIIGGCCGTTPNHIRAMAEAVKGLPPVTEKRVKIRKKAALAVQNERTEPALDDLAKQKRSII
VELDPPKQLNFEKFLHAAEELKSAGIDALTLADNSLATPRISNVACGALLKQRLDMRSLIHITCRDRNLIGLQSHLMGLD
TLGLSDVLAITGDPSKIGDFPGATSVYDLTSFDLISLIKQFNEGLSYSGKPLGKKTNFSVAAAFNPNVRHIDKAVKRLEK
KIACGADYFISQPVYSEEQLIKIHKETRHLETPIYIGVMPLTSSRNAEFIHNEIPGIKLSDSIREKMALAGNDKQKQAEE
GLAIARSLLDAACDLFNGIYLITPFLRSDLTAELTAYIHQKEKEKTNV

Specific function: Terminal step in the de novo biosynthesis of methionine. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Hcy-binding domain [H]

Homologues:

Organism=Homo sapiens, GI13162290, Length=321, Percent_Identity=30.2180685358255, Blast_Score=115, Evalue=9e-26,
Organism=Homo sapiens, GI169790923, Length=327, Percent_Identity=27.217125382263, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI157266337, Length=292, Percent_Identity=29.7945205479452, Blast_Score=97, Evalue=5e-20,
Organism=Escherichia coli, GI1790450, Length=322, Percent_Identity=29.5031055900621, Blast_Score=125, Evalue=7e-30,
Organism=Escherichia coli, GI1786456, Length=301, Percent_Identity=27.906976744186, Blast_Score=84, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17535405, Length=324, Percent_Identity=29.0123456790123, Blast_Score=121, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6324982, Length=318, Percent_Identity=25.7861635220126, Blast_Score=85, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6322966, Length=314, Percent_Identity=26.4331210191083, Blast_Score=75, Evalue=4e-14,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR003726 [H]

Pfam domain/function: PF02219 MTHFR; PF02574 S-methyl_trans [H]

EC number: =2.1.1.10; =1.5.1.20 [H]

Molecular weight: Translated: 67589; Mature: 67458

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS50970 HCY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFLEDIKNKTLIADGAMGTLLYSYGIDRCFEELNISKPEEIRRVHEAYVQAGADIIQTN
CCCHHHHCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEC
TYGANFIKLSRYGLEDETKQINEKAVRLARAAAGSAYVLGTLGGIRTFNKNSYSLEDIKR
CCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEECCCCCCCHHHHHH
SFREQLYLLLNEQPDGLLLETYYDLEEAREVLKIARKETELPIILNVSMHEEGVLQDGTP
HHHHHHHHHCCCCCCCEEEHHHHCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCC
LADGLKQLASLGADVVGINCRLGPYHMIRALEEVPLLEDAYLSVYPNSSLPSLVEGRLVY
HHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHCCEEEE
ETDDEYFRESAEEFRNQGARIIGGCCGTTPNHIRAMAEAVKGLPPVTEKRVKIRKKAALA
ECCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
VQNERTEPALDDLAKQKRSIIVELDPPKQLNFEKFLHAAEELKSAGIDALTLADNSLATP
HCCCCCCHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCC
RISNVACGALLKQRLDMRSLIHITCRDRNLIGLQSHLMGLDTLGLSDVLAITGDPSKIGD
CHHHHHHHHHHHHHHCHHHEEEEEECCCCEEHHHHHHHHHHHCCCCCEEEECCCCHHCCC
FPGATSVYDLTSFDLISLIKQFNEGLSYSGKPLGKKTNFSVAAAFNPNVRHIDKAVKRLE
CCCCCCEEECHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHH
KKIACGADYFISQPVYSEEQLIKIHKETRHLETPIYIGVMPLTSSRNAEFIHNEIPGIKL
HHHHCCCHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHCCCCCEE
SDSIREKMALAGNDKQKQAEEGLAIARSLLDAACDLFNGIYLITPFLRSDLTAELTAYIH
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHEECHHHHCCHHHHHHHHHH
QKEKEKTNV
HHHHHCCCC
>Mature Secondary Structure 
GFLEDIKNKTLIADGAMGTLLYSYGIDRCFEELNISKPEEIRRVHEAYVQAGADIIQTN
CCHHHHCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEC
TYGANFIKLSRYGLEDETKQINEKAVRLARAAAGSAYVLGTLGGIRTFNKNSYSLEDIKR
CCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEECCCCCCCHHHHHH
SFREQLYLLLNEQPDGLLLETYYDLEEAREVLKIARKETELPIILNVSMHEEGVLQDGTP
HHHHHHHHHCCCCCCCEEEHHHHCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCC
LADGLKQLASLGADVVGINCRLGPYHMIRALEEVPLLEDAYLSVYPNSSLPSLVEGRLVY
HHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHCCEEEE
ETDDEYFRESAEEFRNQGARIIGGCCGTTPNHIRAMAEAVKGLPPVTEKRVKIRKKAALA
ECCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
VQNERTEPALDDLAKQKRSIIVELDPPKQLNFEKFLHAAEELKSAGIDALTLADNSLATP
HCCCCCCHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCC
RISNVACGALLKQRLDMRSLIHITCRDRNLIGLQSHLMGLDTLGLSDVLAITGDPSKIGD
CHHHHHHHHHHHHHHCHHHEEEEEECCCCEEHHHHHHHHHHHCCCCCEEEECCCCHHCCC
FPGATSVYDLTSFDLISLIKQFNEGLSYSGKPLGKKTNFSVAAAFNPNVRHIDKAVKRLE
CCCCCCEEECHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHH
KKIACGADYFISQPVYSEEQLIKIHKETRHLETPIYIGVMPLTSSRNAEFIHNEIPGIKL
HHHHCCCHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHCCCCCEE
SDSIREKMALAGNDKQKQAEEGLAIARSLLDAACDLFNGIYLITPFLRSDLTAELTAYIH
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHEECHHHHCCHHHHHHHHHH
QKEKEKTNV
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353932; 9384377 [H]