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The map label for this gene is melA [H]

Identifier: 52784915

GI number: 52784915

Start: 1149016

End: 1150314

Strand: Direct

Name: melA [H]

Synonym: BLi01143

Alternate gene names: 52784915

Gene position: 1149016-1150314 (Clockwise)

Preceding gene: 52784914

Following gene: 52784917

Centisome position: 27.21

GC content: 47.04

Gene sequence:

>1299_bases
ATGACTAAAATTGCTTTTATCGGAGCTGGAAGCACAATTTTTGCGAAAAATGTGCTGGGGGACTGTCTGATGGTTCCGGC
GGTCAACGGATTTGAATTTGCTTTGTATGATATCGATCCGAAGCGCCTCAAAGAATCTAAAGATATGCTTGAGCATTTAA
AAAACCGCTATAATGACACGGTATCGATCCGCGCCTATGACGACAGAAAGGAAGCTTTGGCGGGAGCGAAATACGTGATC
AATGCCATTCAGGTCGGCGGCTATAAACCGGGCACAGTCATCGATTTTGAGATTCCGAAAAAATACGGCCTCAAGCAGAC
GATTGCCGATACGGTCGGAATCGGCGGGATATTCAGGGCGCTGCGGACGATTCCCGTTTTATTGGATATGGCAAAAGACA
TCGAAAGCGCGGCGCCCGACGCCTGGCTCTTAAATTACACAAACCCGATGGCTTCTTTAACCGGTGCGCTTCTCCGCTAC
ACGGACGTGAAAACGGTCGGTCTCTGCCACAGCGTTCAAGTATGCACAAAAGATCTTTTCAAATCCCTCGGCATGAAGCA
TGAACGAATCGAAGAAAAAATCGCCGGCATCAATCATATGGCCTGGCTTCTTGAAGTCAAGCAAGACGGAAGAGACTTGT
ATCCAGAAATTAAAAAGCGGGCAAAAGAAAAACAGAAAACGAAACATGGTGACATGGTCAGGTTTGAACTGATGGATAAA
TTCGGCTACTATGTGACCGAATCGTCCGAACATAACGCGGAGTACCACCCTTATTTTATTAAAAGCCGTTATCCCGAACT
GATTGAAGAACTGAATATCCCGATTGACGAATATTTGAGAAGGTGCGAGAAGCAGATTCAAAATTGGGAAAAGATGCGCG
GCGAAATCGTCGGCAATCGGCATTTAACCCACGAGCGGTCAAACGAATACGGCTCAAGAATCATAGAAGCGATGGAAACC
GGCCAGCCGTTTACATTCGGCGGTAATGTCTTGAATAAAGGATTGATTACAAATCTTCCGGAAAAAGCCGTCGTCGAAGT
GACGTGCGTCGCTGAGCGGAACCGGATCACTCCGTGCTACGCGGGCGAGCTGCCAGAACAGCTGGCTGCTTTAAATCGCA
CCAATATCAATACACAGCTGTTAACGATTGAAGCGGCAACAACAAAAAGAAAAGAGCACATTTACCATGCTGCATTGCTT
GATCCGCATACGGCTGCGGAATTATCGATGGATGATATCGTAAGCATGTGCGATGAATTGATCGAAGCACACGGCGACTG
GCTGCCGGAATTTGAATAA

Upstream 100 bases:

>100_bases
GACGATTCTATTTTTATTCCTGCAAAAGTACTTTATAAAAGGATTATCAGCAGGAGCGCTAAAAGGCTAGCGAAAATTTC
ACGGGGAAAGGAAGATATGA

Downstream 100 bases:

>100_bases
AAAAATAAAGCCTGAGCGAAGGGATCAGCCTTCAACTCTGGCTTTTTTCACGCGAGGGTGTGTATATTTTTCCGATTTTT
GATGAAGCAATCGGAAGAAT

Product: MelA

Products: NA

Alternate protein names: Melibiase [H]

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MTKIAFIGAGSTIFAKNVLGDCLMVPAVNGFEFALYDIDPKRLKESKDMLEHLKNRYNDTVSIRAYDDRKEALAGAKYVI
NAIQVGGYKPGTVIDFEIPKKYGLKQTIADTVGIGGIFRALRTIPVLLDMAKDIESAAPDAWLLNYTNPMASLTGALLRY
TDVKTVGLCHSVQVCTKDLFKSLGMKHERIEEKIAGINHMAWLLEVKQDGRDLYPEIKKRAKEKQKTKHGDMVRFELMDK
FGYYVTESSEHNAEYHPYFIKSRYPELIEELNIPIDEYLRRCEKQIQNWEKMRGEIVGNRHLTHERSNEYGSRIIEAMET
GQPFTFGGNVLNKGLITNLPEKAVVEVTCVAERNRITPCYAGELPEQLAALNRTNINTQLLTIEAATTKRKEHIYHAALL
DPHTAAELSMDDIVSMCDELIEAHGDWLPEFE

Sequences:

>Translated_432_residues
MTKIAFIGAGSTIFAKNVLGDCLMVPAVNGFEFALYDIDPKRLKESKDMLEHLKNRYNDTVSIRAYDDRKEALAGAKYVI
NAIQVGGYKPGTVIDFEIPKKYGLKQTIADTVGIGGIFRALRTIPVLLDMAKDIESAAPDAWLLNYTNPMASLTGALLRY
TDVKTVGLCHSVQVCTKDLFKSLGMKHERIEEKIAGINHMAWLLEVKQDGRDLYPEIKKRAKEKQKTKHGDMVRFELMDK
FGYYVTESSEHNAEYHPYFIKSRYPELIEELNIPIDEYLRRCEKQIQNWEKMRGEIVGNRHLTHERSNEYGSRIIEAMET
GQPFTFGGNVLNKGLITNLPEKAVVEVTCVAERNRITPCYAGELPEQLAALNRTNINTQLLTIEAATTKRKEHIYHAALL
DPHTAAELSMDDIVSMCDELIEAHGDWLPEFE
>Mature_431_residues
TKIAFIGAGSTIFAKNVLGDCLMVPAVNGFEFALYDIDPKRLKESKDMLEHLKNRYNDTVSIRAYDDRKEALAGAKYVIN
AIQVGGYKPGTVIDFEIPKKYGLKQTIADTVGIGGIFRALRTIPVLLDMAKDIESAAPDAWLLNYTNPMASLTGALLRYT
DVKTVGLCHSVQVCTKDLFKSLGMKHERIEEKIAGINHMAWLLEVKQDGRDLYPEIKKRAKEKQKTKHGDMVRFELMDKF
GYYVTESSEHNAEYHPYFIKSRYPELIEELNIPIDEYLRRCEKQIQNWEKMRGEIVGNRHLTHERSNEYGSRIIEAMETG
QPFTFGGNVLNKGLITNLPEKAVVEVTCVAERNRITPCYAGELPEQLAALNRTNINTQLLTIEAATTKRKEHIYHAALLD
PHTAAELSMDDIVSMCDELIEAHGDWLPEFE

Specific function: Unknown

COG id: COG1486

COG function: function code G; Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 4 family [H]

Homologues:

Organism=Escherichia coli, GI1790560, Length=443, Percent_Identity=47.4040632054176, Blast_Score=396, Evalue=1e-111,
Organism=Escherichia coli, GI1788029, Length=418, Percent_Identity=27.2727272727273, Blast_Score=115, Evalue=5e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019802
- InterPro:   IPR001088
- InterPro:   IPR022616
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02056 Glyco_hydro_4; PF11975 Glyco_hydro_4C [H]

EC number: =3.2.1.22 [H]

Molecular weight: Translated: 48976; Mature: 48845

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS01324 GLYCOSYL_HYDROL_F4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKIAFIGAGSTIFAKNVLGDCLMVPAVNGFEFALYDIDPKRLKESKDMLEHLKNRYNDT
CCEEEEEECCCHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCE
VSIRAYDDRKEALAGAKYVINAIQVGGYKPGTVIDFEIPKKYGLKQTIADTVGIGGIFRA
EEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHCHHHHHHHHHCHHHHHHH
LRTIPVLLDMAKDIESAAPDAWLLNYTNPMASLTGALLRYTDVKTVGLCHSVQVCTKDLF
HHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
KSLGMKHERIEEKIAGINHMAWLLEVKQDGRDLYPEIKKRAKEKQKTKHGDMVRFELMDK
HHHCCCHHHHHHHHHCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCEEHHHHHHH
FGYYVTESSEHNAEYHPYFIKSRYPELIEELNIPIDEYLRRCEKQIQNWEKMRGEIVGNR
HCCEEECCCCCCCCCCCEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
HLTHERSNEYGSRIIEAMETGQPFTFGGNVLNKGLITNLPEKAVVEVTCVAERNRITPCY
CCCCHHHHHHHHHHHHHHHCCCCEEECCHHHHCCHHCCCCCHHEEEEEEEECCCCCCCCC
AGELPEQLAALNRTNINTQLLTIEAATTKRKEHIYHAALLDPHTAAELSMDDIVSMCDEL
CCCCHHHHHHHHCCCCCEEEEEEEEHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHH
IEAHGDWLPEFE
HHHCCCCCCCCC
>Mature Secondary Structure 
TKIAFIGAGSTIFAKNVLGDCLMVPAVNGFEFALYDIDPKRLKESKDMLEHLKNRYNDT
CEEEEEECCCHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCE
VSIRAYDDRKEALAGAKYVINAIQVGGYKPGTVIDFEIPKKYGLKQTIADTVGIGGIFRA
EEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHCHHHHHHHHHCHHHHHHH
LRTIPVLLDMAKDIESAAPDAWLLNYTNPMASLTGALLRYTDVKTVGLCHSVQVCTKDLF
HHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
KSLGMKHERIEEKIAGINHMAWLLEVKQDGRDLYPEIKKRAKEKQKTKHGDMVRFELMDK
HHHCCCHHHHHHHHHCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCEEHHHHHHH
FGYYVTESSEHNAEYHPYFIKSRYPELIEELNIPIDEYLRRCEKQIQNWEKMRGEIVGNR
HCCEEECCCCCCCCCCCEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
HLTHERSNEYGSRIIEAMETGQPFTFGGNVLNKGLITNLPEKAVVEVTCVAERNRITPCY
CCCCHHHHHHHHHHHHHHHCCCCEEECCHHHHCCHHCCCCCHHEEEEEEEECCCCCCCCC
AGELPEQLAALNRTNINTQLLTIEAATTKRKEHIYHAALLDPHTAAELSMDDIVSMCDEL
CCCCHHHHHHHHCCCCCEEEEEEEEHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHH
IEAHGDWLPEFE
HHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]