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The map label for this gene is cpkA [H]

Identifier: 52784906

GI number: 52784906

Start: 1138258

End: 1139190

Strand: Direct

Name: cpkA [H]

Synonym: BLi01134

Alternate gene names: 52784906

Gene position: 1138258-1139190 (Clockwise)

Preceding gene: 52784905

Following gene: 52784907

Centisome position: 26.96

GC content: 40.09

Gene sequence:

>933_bases
ATGGGGAAAAGTGTAGTAATCGCACTAGGAGGTAATGCAATTTTACAGCCAAAACAAGAAGCAACTTATGCTAATCAATT
CAAAAATGTAATCTCTGCAACACGTCATATGATTGAAATTAAAGAAGCAGGACATAAAATTGTGATTACACACGGCAATG
GTCCGCAAGTTGGGAATATTATTGCTCAAAATGAAGCTGCCAAAAATGTCGTTGAACCGCTGCCGATCTATGCTTGTAAC
GCAGAATCACAGGGATTCATTGGTTATATGCTCGAAAAAGCTTTAAAAAACCATATTAAAAAACGGGGCATTACCGCGAA
TGTAGCTACATTTCTAACAATGGTTGAAGTGGACAAGTATGATAAAGCATTTGAAAAACCAACGAAACCAATAGGAGTAT
TTTATTCAGAGGAAGAAGCGAAGAAATTGGAGAAAAAAGGGTTTGCTATGTCAGAAGATGCAGGGCGTGGCTATAGACGA
GTTGTGCCTTCCCCAGAACCGAAAGTGATCCATGGGATAGAAGAGATTCGACGTTTAATTGATGAAACGATTGTTATCTC
CTCGGGCGGCGGCGGTATACCTGTATATCGTGACGACAGCGGTCTATTACATGGTCTTGAAGCAGTAATAGACAAAGATC
GTTCAGGATTAAAGCTGGCACAACAATTAAATGCTGATGTTTTCATGATGTTGACAGACGTACCAAATGTGTTTATCAAT
TTTGGCAAAGAAAATGAGCAAAAACTAAGCCGAATTACAGTAGAAGAAGCAGAACAATATGTGGCAGAAGGTCAATTTCC
TGCTGGCAGTATGCTGCCAAAGATGCAAGCGGCTATAGAATTTGCAAAGTTAGGTAAAGAGGCAATTATTTGTTCATTGA
ACCAAGCGGCAGCTGCACTAGACGGAAAAGCGGGGACGCGCATAACAAAATAA

Upstream 100 bases:

>100_bases
CATCTTTGAAAAGTATAAAAAGTCTTATAAAAGTAGGTTCTTCTTCTGGGATGGATACTTTATATGGAATTTATATGGCT
TTAACAAAGGAGTGAAAGAA

Downstream 100 bases:

>100_bases
GACGATCCGTGTATGTTCATAAGGTGGATAAAAACTAAAATTCCCACCTTTTCAGATATGACGACATTTGATCTGTCATT
CTGATCAAAAATTTCTGAAC

Product: carbamate kinase

Products: NA

Alternate protein names: Carbamate kinase-like carbamoylphosphate synthase [H]

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MGKSVVIALGGNAILQPKQEATYANQFKNVISATRHMIEIKEAGHKIVITHGNGPQVGNIIAQNEAAKNVVEPLPIYACN
AESQGFIGYMLEKALKNHIKKRGITANVATFLTMVEVDKYDKAFEKPTKPIGVFYSEEEAKKLEKKGFAMSEDAGRGYRR
VVPSPEPKVIHGIEEIRRLIDETIVISSGGGGIPVYRDDSGLLHGLEAVIDKDRSGLKLAQQLNADVFMMLTDVPNVFIN
FGKENEQKLSRITVEEAEQYVAEGQFPAGSMLPKMQAAIEFAKLGKEAIICSLNQAAAALDGKAGTRITK

Sequences:

>Translated_310_residues
MGKSVVIALGGNAILQPKQEATYANQFKNVISATRHMIEIKEAGHKIVITHGNGPQVGNIIAQNEAAKNVVEPLPIYACN
AESQGFIGYMLEKALKNHIKKRGITANVATFLTMVEVDKYDKAFEKPTKPIGVFYSEEEAKKLEKKGFAMSEDAGRGYRR
VVPSPEPKVIHGIEEIRRLIDETIVISSGGGGIPVYRDDSGLLHGLEAVIDKDRSGLKLAQQLNADVFMMLTDVPNVFIN
FGKENEQKLSRITVEEAEQYVAEGQFPAGSMLPKMQAAIEFAKLGKEAIICSLNQAAAALDGKAGTRITK
>Mature_309_residues
GKSVVIALGGNAILQPKQEATYANQFKNVISATRHMIEIKEAGHKIVITHGNGPQVGNIIAQNEAAKNVVEPLPIYACNA
ESQGFIGYMLEKALKNHIKKRGITANVATFLTMVEVDKYDKAFEKPTKPIGVFYSEEEAKKLEKKGFAMSEDAGRGYRRV
VPSPEPKVIHGIEEIRRLIDETIVISSGGGGIPVYRDDSGLLHGLEAVIDKDRSGLKLAQQLNADVFMMLTDVPNVFINF
GKENEQKLSRITVEEAEQYVAEGQFPAGSMLPKMQAAIEFAKLGKEAIICSLNQAAAALDGKAGTRITK

Specific function: Carbamate kinase that plays a biosynthetic role in that it produces carbamoyl-phosphate [H]

COG id: COG0549

COG function: function code E; Carbamate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbamate kinase family [H]

Homologues:

Organism=Escherichia coli, GI1789238, Length=316, Percent_Identity=46.2025316455696, Blast_Score=265, Evalue=4e-72,
Organism=Escherichia coli, GI1786516, Length=316, Percent_Identity=41.7721518987342, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI1786732, Length=310, Percent_Identity=40.6451612903226, Blast_Score=191, Evalue=7e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR003964 [H]

Pfam domain/function: PF00696 AA_kinase [H]

EC number: =2.7.2.2 [H]

Molecular weight: Translated: 33752; Mature: 33621

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKSVVIALGGNAILQPKQEATYANQFKNVISATRHMIEIKEAGHKIVITHGNGPQVGNI
CCCEEEEEECCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCE
IAQNEAAKNVVEPLPIYACNAESQGFIGYMLEKALKNHIKKRGITANVATFLTMVEVDKY
EECCHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
DKAFEKPTKPIGVFYSEEEAKKLEKKGFAMSEDAGRGYRRVVPSPEPKVIHGIEEIRRLI
HHHHHCCCCCEEEEECHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHH
DETIVISSGGGGIPVYRDDSGLLHGLEAVIDKDRSGLKLAQQLNADVFMMLTDVPNVFIN
HHEEEEECCCCCCCEEECCCHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEECCCCEEEE
FGKENEQKLSRITVEEAEQYVAEGQFPAGSMLPKMQAAIEFAKLGKEAIICSLNQAAAAL
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHEEEECHHHHHHH
DGKAGTRITK
CCCCCCCCCC
>Mature Secondary Structure 
GKSVVIALGGNAILQPKQEATYANQFKNVISATRHMIEIKEAGHKIVITHGNGPQVGNI
CCEEEEEECCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCE
IAQNEAAKNVVEPLPIYACNAESQGFIGYMLEKALKNHIKKRGITANVATFLTMVEVDKY
EECCHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
DKAFEKPTKPIGVFYSEEEAKKLEKKGFAMSEDAGRGYRRVVPSPEPKVIHGIEEIRRLI
HHHHHCCCCCEEEEECHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHH
DETIVISSGGGGIPVYRDDSGLLHGLEAVIDKDRSGLKLAQQLNADVFMMLTDVPNVFIN
HHEEEEECCCCCCCEEECCCHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEECCCCEEEE
FGKENEQKLSRITVEEAEQYVAEGQFPAGSMLPKMQAAIEFAKLGKEAIICSLNQAAAAL
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHEEEECHHHHHHH
DGKAGTRITK
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371756; 10860751 [H]