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The map label for this gene is apr [H]

Identifier: 52784881

GI number: 52784881

Start: 1108966

End: 1110105

Strand: Reverse

Name: apr [H]

Synonym: BLi01109

Alternate gene names: 52784881

Gene position: 1110105-1108966 (Counterclockwise)

Preceding gene: 52784886

Following gene: 52784876

Centisome position: 26.29

GC content: 50.7

Gene sequence:

>1140_bases
ATGATGAGGAAAAAGAGTTTTTGGCTTGGGATGCTGACGGCCTTAATGCTCGTGTTCACGATGGCCTTCAGCGATTCCGC
GTCTGCTGCTCAGCCGGCGAAAAATGTTGAAAAGGATTATATTGTCGGATTTAAGTCGGGAGTGAAAACCGCATCCGTCA
AAAAGGACATCATCAAAGAGAGCGGCGGAAAAGTGGACAAGCAGTTTAGAATCATCAACGCGGCAAAAGCGAAGCTAGAC
AAAGAAGCGCTTGAGGAAGTCAAAAATGATCCGGATGTCGCTTATGTGGAAGAGGATCACGTAGCTCATGCTTTGGCGCA
AACCGTTCCTTACGGCATTCCTCTCATTAAAGCGGACAAAGTGCAGGCTCAAGGCTACAAGGGAGCGAACGTAAAAGTCG
CCGTCCTGGATACAGGAATCCAAGCTTCTCATCCGGACTTGAACGTAGTCGGCGGAGCAAGCTTCGTAGCTGGCGAAGCT
TATAACACCGACGGCAACGGACACGGCACGCATGTTGCCGGTACAGTAGCTGCGCTTGACAATACAACGGGTGTATTAGG
CGTTGCGCCGAACGTATCCTTGTACGCGGTTAAAGTGCTGAATTCAAGCGGAAGCGGATCTTACAGCGGCATTGTAAGCG
GAATCGAGTGGGCGACGACAAACGGCATGGATGTTATCAACATGAGCCTTGGAGGACCATCAGGCTCAACAGCGATGAAA
CAGGCGGTTGACAATGCATATGCAAGAGGGGTTGTCGTTGTGGCGGCTGCTGGGAACAGCGGATCTTCAGGAAACACGAA
TACAATCGGCTATCCTGCGAAATACGACTCTGTCATCGCAGTTGGCGCGGTAGACTCTAACAGCAACAGAGCTTCATTTT
CCAGCGTCGGAGCAGAGCTTGAAGTCATGGCTCCTGGCGCAGGCGTGTACAGCACTTACCCAACCAGCACTTATGCAACA
TTGAACGGAACGTCAATGGCTTCTCCTCATGTAGCGGGAGCAGCAGCTTTGATCTTGTCAAAACATCCGAACCTTTCAGC
TTCACAAGTCCGCAACCGTCTCTCCAGTACGGCGACTTATTTGGGAAGCTCCTTCTACTATGGAAAAGGTCTGATCAATG
TCGAAGCTGCCGCTCAATAA

Upstream 100 bases:

>100_bases
TAATTCATTTTTTTTCTATAATAAATTAACAGAATAATTAGAATAGAGTATATTATTCTTCTATTTCAATTATTCTGAAT
AAAACGGAGGAGAGTGAGTA

Downstream 100 bases:

>100_bases
CATATTCTAACAAATGGCATATAGAAAAAGCTAGTGTTTTTAGCACTAGCTTTTTCTTCATTCAGTTGAAGACTGTTCAA
TATTTTGAATCCGTTCCATT

Product: subtilisin carlsberg precursor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MMRKKSFWLGMLTALMLVFTMAFSDSASAAQPAKNVEKDYIVGFKSGVKTASVKKDIIKESGGKVDKQFRIINAAKAKLD
KEALEEVKNDPDVAYVEEDHVAHALAQTVPYGIPLIKADKVQAQGYKGANVKVAVLDTGIQASHPDLNVVGGASFVAGEA
YNTDGNGHGTHVAGTVAALDNTTGVLGVAPNVSLYAVKVLNSSGSGSYSGIVSGIEWATTNGMDVINMSLGGPSGSTAMK
QAVDNAYARGVVVVAAAGNSGSSGNTNTIGYPAKYDSVIAVGAVDSNSNRASFSSVGAELEVMAPGAGVYSTYPTSTYAT
LNGTSMASPHVAGAAALILSKHPNLSASQVRNRLSSTATYLGSSFYYGKGLINVEAAAQ

Sequences:

>Translated_379_residues
MMRKKSFWLGMLTALMLVFTMAFSDSASAAQPAKNVEKDYIVGFKSGVKTASVKKDIIKESGGKVDKQFRIINAAKAKLD
KEALEEVKNDPDVAYVEEDHVAHALAQTVPYGIPLIKADKVQAQGYKGANVKVAVLDTGIQASHPDLNVVGGASFVAGEA
YNTDGNGHGTHVAGTVAALDNTTGVLGVAPNVSLYAVKVLNSSGSGSYSGIVSGIEWATTNGMDVINMSLGGPSGSTAMK
QAVDNAYARGVVVVAAAGNSGSSGNTNTIGYPAKYDSVIAVGAVDSNSNRASFSSVGAELEVMAPGAGVYSTYPTSTYAT
LNGTSMASPHVAGAAALILSKHPNLSASQVRNRLSSTATYLGSSFYYGKGLINVEAAAQ
>Mature_379_residues
MMRKKSFWLGMLTALMLVFTMAFSDSASAAQPAKNVEKDYIVGFKSGVKTASVKKDIIKESGGKVDKQFRIINAAKAKLD
KEALEEVKNDPDVAYVEEDHVAHALAQTVPYGIPLIKADKVQAQGYKGANVKVAVLDTGIQASHPDLNVVGGASFVAGEA
YNTDGNGHGTHVAGTVAALDNTTGVLGVAPNVSLYAVKVLNSSGSGSYSGIVSGIEWATTNGMDVINMSLGGPSGSTAMK
QAVDNAYARGVVVVAAAGNSGSSGNTNTIGYPAKYDSVIAVGAVDSNSNRASFSSVGAELEVMAPGAGVYSTYPTSTYAT
LNGTSMASPHVAGAAALILSKHPNLSASQVRNRLSSTATYLGSSFYYGKGLINVEAAAQ

Specific function: Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides [H]

COG id: COG1404

COG function: function code O; Subtilisin-like serine proteases

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S8 family [H]

Homologues:

Organism=Homo sapiens, GI4506775, Length=301, Percent_Identity=31.8936877076412, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI31317307, Length=303, Percent_Identity=30.6930693069307, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI27894285, Length=371, Percent_Identity=25.8760107816712, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI20336190, Length=371, Percent_Identity=25.8760107816712, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI20336188, Length=363, Percent_Identity=25.3443526170799, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI20336180, Length=371, Percent_Identity=25.8760107816712, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI4505577, Length=371, Percent_Identity=25.8760107816712, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI20336184, Length=371, Percent_Identity=25.8760107816712, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI20336182, Length=371, Percent_Identity=25.8760107816712, Blast_Score=67, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6320775, Length=292, Percent_Identity=35.2739726027397, Blast_Score=129, Evalue=7e-31,
Organism=Saccharomyces cerevisiae, GI6324576, Length=292, Percent_Identity=34.2465753424658, Blast_Score=125, Evalue=8e-30,
Organism=Saccharomyces cerevisiae, GI6319893, Length=315, Percent_Identity=32.3809523809524, Blast_Score=115, Evalue=8e-27,
Organism=Drosophila melanogaster, GI45550681, Length=239, Percent_Identity=30.5439330543933, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24653270, Length=255, Percent_Identity=31.7647058823529, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI17137360, Length=255, Percent_Identity=31.7647058823529, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI17136192, Length=263, Percent_Identity=28.5171102661597, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000209
- InterPro:   IPR022398
- InterPro:   IPR015500
- InterPro:   IPR009020
- InterPro:   IPR010259 [H]

Pfam domain/function: PF05922 Inhibitor_I9; PF00082 Peptidase_S8 [H]

EC number: =3.4.21.62 [H]

Molecular weight: Translated: 38904; Mature: 38904

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: PS00136 SUBTILASE_ASP ; PS00137 SUBTILASE_HIS ; PS00138 SUBTILASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRKKSFWLGMLTALMLVFTMAFSDSASAAQPAKNVEKDYIVGFKSGVKTASVKKDIIKE
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCEEEEECCCCCHHHHHHHHHHH
SGGKVDKQFRIINAAKAKLDKEALEEVKNDPDVAYVEEDHVAHALAQTVPYGIPLIKADK
CCCCCCHHEEEHHHHHHHCCHHHHHHHCCCCCEEEEEHHHHHHHHHHHCCCCCCEEECCC
VQAQGYKGANVKVAVLDTGIQASHPDLNVVGGASFVAGEAYNTDGNGHGTHVAGTVAALD
EEECCCCCCCEEEEEEECCCCCCCCCEEEECCCCEEECCCCCCCCCCCCCEEEEEEEEEC
NTTGVLGVAPNVSLYAVKVLNSSGSGSYSGIVSGIEWATTNGMDVINMSLGGPSGSTAMK
CCCCEEEECCCCEEEEEEEECCCCCCCCHHHHCCCEEEECCCCEEEEEECCCCCCHHHHH
QAVDNAYARGVVVVAAAGNSGSSGNTNTIGYPAKYDSVIAVGAVDSNSNRASFSSVGAEL
HHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCHHHCCCEE
EVMAPGAGVYSTYPTSTYATLNGTSMASPHVAGAAALILSKHPNLSASQVRNRLSSTATY
EEECCCCCCEECCCCCEEEEECCCCCCCCCHHHHEEEEEECCCCCCHHHHHHHHHHHHHH
LGSSFYYGKGLINVEAAAQ
HCCCEEECCCEEEEEECCC
>Mature Secondary Structure
MMRKKSFWLGMLTALMLVFTMAFSDSASAAQPAKNVEKDYIVGFKSGVKTASVKKDIIKE
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCEEEEECCCCCHHHHHHHHHHH
SGGKVDKQFRIINAAKAKLDKEALEEVKNDPDVAYVEEDHVAHALAQTVPYGIPLIKADK
CCCCCCHHEEEHHHHHHHCCHHHHHHHCCCCCEEEEEHHHHHHHHHHHCCCCCCEEECCC
VQAQGYKGANVKVAVLDTGIQASHPDLNVVGGASFVAGEAYNTDGNGHGTHVAGTVAALD
EEECCCCCCCEEEEEEECCCCCCCCCEEEECCCCEEECCCCCCCCCCCCCEEEEEEEEEC
NTTGVLGVAPNVSLYAVKVLNSSGSGSYSGIVSGIEWATTNGMDVINMSLGGPSGSTAMK
CCCCEEEECCCCEEEEEEEECCCCCCCCHHHHCCCEEEECCCCEEEEEECCCCCCHHHHH
QAVDNAYARGVVVVAAAGNSGSSGNTNTIGYPAKYDSVIAVGAVDSNSNRASFSSVGAEL
HHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCHHHCCCEE
EVMAPGAGVYSTYPTSTYATLNGTSMASPHVAGAAALILSKHPNLSASQVRNRLSSTATY
EEECCCCCCEECCCCCEEEEECCCCCCCCCHHHHEEEEEECCCCCCHHHHHHHHHHHHHH
LGSSFYYGKGLINVEAAAQ
HCCCEEECCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3001653; 4967581; 8512925; 9425066 [H]