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The map label for this gene is yhfJ [H]

Identifier: 52784874

GI number: 52784874

Start: 1102758

End: 1103747

Strand: Direct

Name: yhfJ [H]

Synonym: BLi01102

Alternate gene names: 52784874

Gene position: 1102758-1103747 (Clockwise)

Preceding gene: 52784873

Following gene: 52784875

Centisome position: 26.12

GC content: 44.14

Gene sequence:

>990_bases
ATGCTGTTTATCGATAATCAAAATATTACAGATCCGAGGATCAACTTGGCGATTGAGGAGTACTGCTTAAAGCATCTCGA
TCCTGAAGAGACGTATCTGCTGTTTTATATCAACCAGCCGTCGATCATCATCGGCAAAAATCAAAATACGATCGAGGAAA
TCAACACAAAATATGTAGATGAAAACGGGATCATTGTCGTCCGCAGGCTGTCAGGCGGCGGAGCTGTCTACCATGATCTC
GGGAACTTGAACTTCAGTTTTATTACGAAGGACGACGGCAACAGCTTCCACAACTTCAAGAAGTTTACTGAGCCCGTTGT
GGCCGCTTTAAAACGGCTTGGCGTCGATGCGGAGCTGAGCGGCCGAAACGATTTGATGGCAAACGGCCGGAAAATCTCGG
GAAACGCCCAGTTTTCAACGAAAGGAAGAATGTTCAGCCACGGCACGCTGTTGTTCGATTCTGAGATTGAACATGTCGTA
TCGGCTTTAAAAGTGAAAAAAGATAAAATCGAATCAAAAGGCATTAAATCGATCAGGAGCCGTGTGGCCAATATTAGCGA
GTTTCTCGATCAGAAGATGACGACAGTGGAATTCCGAAGCATGCTTCTCCGCTATATTTTTGATACCGAAGGGGAAATAC
CGGAGTATAAGCTGACTGAAAAAGACTGGAAAATCATTAATCAAATTTCAAAGGAACGCTATCAAAACTGGGATTGGAAC
TATGGGAAGTCTCCGAAATTCAATCTGCAGCATTCTAAACGATTTCAAGCTGGATCGGTCGACATCCGCCTCGAAGTGCA
AAAAGGCGTGATCCGCGAGTGCAAAATATTCGGTGACTTTTTCGGAGTCGGAGATGTCAGCGACATCGAAGAGAAGCTGA
CAGGCGTGCAGTATGAAAGAAAAGCGATTGAAGAAGCTCTTCAAGGCGTGGACATTAAGCATTACTTCGGCAATATTCAA
AAAGAAGAATTTTTGGATCTTGTCTACTAA

Upstream 100 bases:

>100_bases
CCAAAAACTGATAGAAGAGGCCCAAACCGTTTATAAAGGTGCGGTTGTTCTGGCGCACACCGGTTATGTGTGGGGAGAAT
AGAAGGAGGAACTGACAAAC

Downstream 100 bases:

>100_bases
TAGAGAGGCTGCGGTTTCCGCGGCCCCTTTTTTAACATGAGAGGAATGGAAGATATTTCCCGGCAGCCCTTGAGTGTTTT
CTGTTCATTTACTATAATGG

Product: YhfJ

Products: NA

Alternate protein names: Lipoate--protein ligase [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MLFIDNQNITDPRINLAIEEYCLKHLDPEETYLLFYINQPSIIIGKNQNTIEEINTKYVDENGIIVVRRLSGGGAVYHDL
GNLNFSFITKDDGNSFHNFKKFTEPVVAALKRLGVDAELSGRNDLMANGRKISGNAQFSTKGRMFSHGTLLFDSEIEHVV
SALKVKKDKIESKGIKSIRSRVANISEFLDQKMTTVEFRSMLLRYIFDTEGEIPEYKLTEKDWKIINQISKERYQNWDWN
YGKSPKFNLQHSKRFQAGSVDIRLEVQKGVIRECKIFGDFFGVGDVSDIEEKLTGVQYERKAIEEALQGVDIKHYFGNIQ
KEEFLDLVY

Sequences:

>Translated_329_residues
MLFIDNQNITDPRINLAIEEYCLKHLDPEETYLLFYINQPSIIIGKNQNTIEEINTKYVDENGIIVVRRLSGGGAVYHDL
GNLNFSFITKDDGNSFHNFKKFTEPVVAALKRLGVDAELSGRNDLMANGRKISGNAQFSTKGRMFSHGTLLFDSEIEHVV
SALKVKKDKIESKGIKSIRSRVANISEFLDQKMTTVEFRSMLLRYIFDTEGEIPEYKLTEKDWKIINQISKERYQNWDWN
YGKSPKFNLQHSKRFQAGSVDIRLEVQKGVIRECKIFGDFFGVGDVSDIEEKLTGVQYERKAIEEALQGVDIKHYFGNIQ
KEEFLDLVY
>Mature_329_residues
MLFIDNQNITDPRINLAIEEYCLKHLDPEETYLLFYINQPSIIIGKNQNTIEEINTKYVDENGIIVVRRLSGGGAVYHDL
GNLNFSFITKDDGNSFHNFKKFTEPVVAALKRLGVDAELSGRNDLMANGRKISGNAQFSTKGRMFSHGTLLFDSEIEHVV
SALKVKKDKIESKGIKSIRSRVANISEFLDQKMTTVEFRSMLLRYIFDTEGEIPEYKLTEKDWKIINQISKERYQNWDWN
YGKSPKFNLQHSKRFQAGSVDIRLEVQKGVIRECKIFGDFFGVGDVSDIEEKLTGVQYERKAIEEALQGVDIKHYFGNIQ
KEEFLDLVY

Specific function: Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes [H]

COG id: COG0095

COG function: function code H; Lipoate-protein ligase A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lplA family [H]

Homologues:

Organism=Homo sapiens, GI7706252, Length=272, Percent_Identity=32.3529411764706, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI21729884, Length=272, Percent_Identity=32.3529411764706, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI21729882, Length=272, Percent_Identity=32.3529411764706, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI21729880, Length=272, Percent_Identity=32.3529411764706, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI21729878, Length=272, Percent_Identity=32.3529411764706, Blast_Score=120, Evalue=2e-27,
Organism=Escherichia coli, GI1790846, Length=307, Percent_Identity=33.5504885993485, Blast_Score=178, Evalue=4e-46,
Organism=Caenorhabditis elegans, GI71981909, Length=249, Percent_Identity=32.9317269076305, Blast_Score=112, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6322415, Length=278, Percent_Identity=30.9352517985612, Blast_Score=123, Evalue=3e-29,
Organism=Drosophila melanogaster, GI28573464, Length=282, Percent_Identity=29.4326241134752, Blast_Score=120, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR005107
- InterPro:   IPR019491
- InterPro:   IPR004562 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB; PF10437 Lip_prot_lig_C [H]

EC number: =2.7.7.63 [H]

Molecular weight: Translated: 37905; Mature: 37905

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFIDNQNITDPRINLAIEEYCLKHLDPEETYLLFYINQPSIIIGKNQNTIEEINTKYVD
CEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEECCCCHHHHHHHEEEC
ENGIIVVRRLSGGGAVYHDLGNLNFSFITKDDGNSFHNFKKFTEPVVAALKRLGVDAELS
CCCEEEEEEECCCCEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
GRNDLMANGRKISGNAQFSTKGRMFSHGTLLFDSEIEHVVSALKVKKDKIESKGIKSIRS
CCCCHHCCCEEECCCCEECCCCCEEECCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVANISEFLDQKMTTVEFRSMLLRYIFDTEGEIPEYKLTEKDWKIINQISKERYQNWDWN
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECHHHHHHHHHHHHHHHCCCCCC
YGKSPKFNLQHSKRFQAGSVDIRLEVQKGVIRECKIFGDFFGVGDVSDIEEKLTGVQYER
CCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHH
KAIEEALQGVDIKHYFGNIQKEEFLDLVY
HHHHHHHHCCCHHHHHCCCCHHHHHHHCC
>Mature Secondary Structure
MLFIDNQNITDPRINLAIEEYCLKHLDPEETYLLFYINQPSIIIGKNQNTIEEINTKYVD
CEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEECCCCHHHHHHHEEEC
ENGIIVVRRLSGGGAVYHDLGNLNFSFITKDDGNSFHNFKKFTEPVVAALKRLGVDAELS
CCCEEEEEEECCCCEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
GRNDLMANGRKISGNAQFSTKGRMFSHGTLLFDSEIEHVVSALKVKKDKIESKGIKSIRS
CCCCHHCCCEEECCCCEECCCCCEEECCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVANISEFLDQKMTTVEFRSMLLRYIFDTEGEIPEYKLTEKDWKIINQISKERYQNWDWN
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECHHHHHHHHHHHHHHHCCCCCC
YGKSPKFNLQHSKRFQAGSVDIRLEVQKGVIRECKIFGDFFGVGDVSDIEEKLTGVQYER
CCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHH
KAIEEALQGVDIKHYFGNIQKEEFLDLVY
HHHHHHHHCCCHHHHHCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]