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The map label for this gene is yfiF [H]

Identifier: 52784663

GI number: 52784663

Start: 885537

End: 886382

Strand: Reverse

Name: yfiF [H]

Synonym: BLi00865

Alternate gene names: 52784663

Gene position: 886382-885537 (Counterclockwise)

Preceding gene: 52784672

Following gene: 52784658

Centisome position: 20.99

GC content: 45.39

Gene sequence:

>846_bases
ATGCTCCATCCTAAATATTTCATAGCCGAGCATTCTTTTTCGATTCAATATATGCAGAGAAAAGGATTTACCGAGATGAA
CGCGCCGCATGTTCATGAAGGCTATGAACTGTATTATCTGCTGAGCGGAGAACGCACGTATTTTATTAACCAAAGCGTCT
ATACCGCGGAAAAAGGCACGATGATCATCATCAATCCCCACGACCTCCACCGAACGTCAAGCACGGAAGTTCAAGAGTTC
GAACGCATCCTGATTAATTTCTCGCACGATTTCATAGAACCATTGCTGAAGTGCAGCCATTATCGGCTTCTTCCGTTTCC
GGAAAATCAAAACTTCATCGCATTTTCCCTGCGTGAACAGGCCGCAGTCGAACATATGCTGATGGAGATGAAAAAGGAGT
GCAAGCTGAAAAAAGAAGGACACGAGGATGTCGTCAGGGCGCTGCTGTTTCAGCTGCTTTTCACCATTTACCGGAGCCTT
GCGGGCGGCGGCCGCAATCAAACACGGCCGCTTCACCCGATGGATGAAAAAATCTCGGAAATCGCTTCATATATCAATTG
CAGCTTTCAAGAAACGATGACGTTAGACGGATTGGCCGGACAATTTTATATCAGCCCATCCTATTTAAGCAGAGTGTTTA
AAAAAGTGACCGGCTTTCATCTTCAGGAGTATATCCGGCACATCAGGATCCGCGAAGCGAAAAAACGGCTTCGTGATACA
GACGAGAAAATATTGGACATCGCTGCGGATACCGGTTTTAAGCATATCAGCCATTTCAATAAAGTGTTTAAAGAGATCGT
CGGCATCCCGCCGCTTCAATACAGGAAAAAAAGCAGAGACATTTAA

Upstream 100 bases:

>100_bases
CAGCCCGCGGAAAAAACCAAGACCAAGATCAGGCAACATAACTTTTTCATACGATCCCTCCCGTTTGTTAAGAAAACGCT
CTCAAGGAGGAATGCCCGAC

Downstream 100 bases:

>100_bases
TATGCAATATAGCGGAACCAGTCAAAATCAGCGGCAGCCTTTCTGCCGCTTCCGCCGTTATTGGCGAATATCCCGACCAT
CGTTCCCGTATGTCCCTTTG

Product: YfiF

Products: NA

Alternate protein names: L-rhamnose operon regulatory protein rhaS [H]

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MLHPKYFIAEHSFSIQYMQRKGFTEMNAPHVHEGYELYYLLSGERTYFINQSVYTAEKGTMIIINPHDLHRTSSTEVQEF
ERILINFSHDFIEPLLKCSHYRLLPFPENQNFIAFSLREQAAVEHMLMEMKKECKLKKEGHEDVVRALLFQLLFTIYRSL
AGGGRNQTRPLHPMDEKISEIASYINCSFQETMTLDGLAGQFYISPSYLSRVFKKVTGFHLQEYIRHIRIREAKKRLRDT
DEKILDIAADTGFKHISHFNKVFKEIVGIPPLQYRKKSRDI

Sequences:

>Translated_281_residues
MLHPKYFIAEHSFSIQYMQRKGFTEMNAPHVHEGYELYYLLSGERTYFINQSVYTAEKGTMIIINPHDLHRTSSTEVQEF
ERILINFSHDFIEPLLKCSHYRLLPFPENQNFIAFSLREQAAVEHMLMEMKKECKLKKEGHEDVVRALLFQLLFTIYRSL
AGGGRNQTRPLHPMDEKISEIASYINCSFQETMTLDGLAGQFYISPSYLSRVFKKVTGFHLQEYIRHIRIREAKKRLRDT
DEKILDIAADTGFKHISHFNKVFKEIVGIPPLQYRKKSRDI
>Mature_281_residues
MLHPKYFIAEHSFSIQYMQRKGFTEMNAPHVHEGYELYYLLSGERTYFINQSVYTAEKGTMIIINPHDLHRTSSTEVQEF
ERILINFSHDFIEPLLKCSHYRLLPFPENQNFIAFSLREQAAVEHMLMEMKKECKLKKEGHEDVVRALLFQLLFTIYRSL
AGGGRNQTRPLHPMDEKISEIASYINCSFQETMTLDGLAGQFYISPSYLSRVFKKVTGFHLQEYIRHIRIREAKKRLRDT
DEKILDIAADTGFKHISHFNKVFKEIVGIPPLQYRKKSRDI

Specific function: Activates expression of the rhaBAD and rhaT operons [H]

COG id: COG2207

COG function: function code K; AraC-type DNA-binding domain-containing proteins

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 HTH araC/xylS-type DNA-binding domains [H]

Homologues:

Organism=Escherichia coli, GI1790559, Length=102, Percent_Identity=32.3529411764706, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1790391, Length=97, Percent_Identity=32.9896907216495, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR003313
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060 [H]

Pfam domain/function: PF02311 AraC_binding; PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 33101; Mature: 33101

Theoretical pI: Translated: 8.94; Mature: 8.94

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHPKYFIAEHSFSIQYMQRKGFTEMNAPHVHEGYELYYLLSGERTYFINQSVYTAEKGT
CCCCCEEEECCCCHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCEEEEECCCEEEECCCE
MIIINPHDLHRTSSTEVQEFERILINFSHDFIEPLLKCSHYRLLPFPENQNFIAFSLREQ
EEEECCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCEEECCCCCCCEEEEEHHHH
AAVEHMLMEMKKECKLKKEGHEDVVRALLFQLLFTIYRSLAGGGRNQTRPLHPMDEKISE
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
IASYINCSFQETMTLDGLAGQFYISPSYLSRVFKKVTGFHLQEYIRHIRIREAKKRLRDT
HHHHHCCCHHHHEEECCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
DEKILDIAADTGFKHISHFNKVFKEIVGIPPLQYRKKSRDI
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCC
>Mature Secondary Structure
MLHPKYFIAEHSFSIQYMQRKGFTEMNAPHVHEGYELYYLLSGERTYFINQSVYTAEKGT
CCCCCEEEECCCCHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCEEEEECCCEEEECCCE
MIIINPHDLHRTSSTEVQEFERILINFSHDFIEPLLKCSHYRLLPFPENQNFIAFSLREQ
EEEECCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCEEECCCCCCCEEEEEHHHH
AAVEHMLMEMKKECKLKKEGHEDVVRALLFQLLFTIYRSLAGGGRNQTRPLHPMDEKISE
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
IASYINCSFQETMTLDGLAGQFYISPSYLSRVFKKVTGFHLQEYIRHIRIREAKKRLRDT
HHHHHCCCHHHHEEECCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
DEKILDIAADTGFKHISHFNKVFKEIVGIPPLQYRKKSRDI
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA