Click here to switch to the map view.

The map label for this gene is purK [H]

Identifier: 52784495

GI number: 52784495

Start: 697456

End: 698700

Strand: Direct

Name: purK [H]

Synonym: BLi00694

Alternate gene names: 52784495

Gene position: 697456-698700 (Clockwise)

Preceding gene: 52784494

Following gene: 52784496

Centisome position: 16.52

GC content: 51.89

Gene sequence:

>1245_bases
TTGCGGCGCAGATTTTGTCAGCTTTTGACAAAGAGCTTGCCGAAAGATTGGACGAACGAAGGGAAAGCATCAGACAAACC
GTGTTGGAAAGCAGTGATCAGCTTGTATAAACAAACGATTTTTCCGGGAGCGACAATCGGCATTATCGGAGGCGGACAGC
TGGGCAAAATGATGGCCGTCGCGGCCAAACAAATGGGCTATAAAATCGCGGTGGTTGATCCTGTTCCTTCATCACCGTGC
GGCCAGGTCGCCGACCTTGAGATTACGGCTAGCTATAATGACGGGGAAGCGATACGGAAGCTTGCCGAAGCGAGCGACGT
GATCACTTACGAATTCGAGAACATCGATTATGACGCGCTGAACTGGCTGAAGGAGCACGCTTACCTTCCGCAGGGGAGCG
AGCTTTTGCTCCTTACGCAAAACAGGGAGACCGAGAAAAAAGCGATTGCATCAGCGGGCTGTAAAGTCGCGCCGTACCGG
ATCATTCACGACCGGGCGGAATTGGAAGCGTCCGTAGCGGAGCTCGGTCTTCCGGCGGTGCTGAAAACTTGCCGCGGCGG
GTATGACGGAAAAGGGCAGCACGTCCTGAAAGAACAAGCACAAATCGGGGAAGCCGCAGCTCTTTTACAGCACGGCACAT
GCATTTTGGAAAGCTGGGTTCCTTTCAAAATGGAATTGTCCGTGATCGTGACAAGATCGGTTCACGGAGAGACAACCGTC
TTTCCGGCGGCAGAAAACATCCATAAACAAAATATTTTATTCCAGAGCATCGTCCCGGCCCGGGTCGATGAGGCGATTCA
GGAACAAGCGGCAGCACTTGCCCTCAGGCTTGCCGAAAAGCTTCAGCTTGTCGGAACACTGGCCGTTGAGCTTTTCCTGA
AGGAAGACGGCGGCCTTCTCGTCAATGAACTGGCGCCGCGGCCGCATAATTCCGGCCATTACACCCTTGATCTTTGCGAA
ACGAGCCAGTTTGAACAGCATATTCGTGCGGTCTGCGGGCTGCCGCTCGGTAAAACGGATCTTCTGAAGGAAGGGATGAT
GGTGAACCTTCTCGGGGACGAAGTGTACATGGCTGAAGAAGATCCCGAACTTTTAAAAGAAGCGAAGCTTTATATATACG
GAAAGCATGAAGTCAAACCAGGCCGCAAAATGGGGCACATCACATTTTTAAGAACACCCGACAATGAATGGATCGGGCAG
ATGACAGAAAAGTGGATTGAAAGAGATGGAGGACGAAAACAATGA

Upstream 100 bases:

>100_bases
AACGGTCTCGATTCGCTGCTTTCAATCGTTCAAATGCCGGGCGGAGTACCCGTTGCGACCGTTGCCATCGGCAAAGCGGG
AGCGGTCAACGCAGGACTGC

Downstream 100 bases:

>100_bases
TCGAACGTTATTCAAGACCGGAAATGGCTGCGATTTGGACAGATGAAAACAGATTCCAAGCATGGCTTGAAGTTGAAATA
CTGGCTTGTGAAGCTTGGGC

Product: phosphoribosylaminoimidazole carboxylase ATPase subunit

Products: NA

Alternate protein names: N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase [H]

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MRRRFCQLLTKSLPKDWTNEGKASDKPCWKAVISLYKQTIFPGATIGIIGGGQLGKMMAVAAKQMGYKIAVVDPVPSSPC
GQVADLEITASYNDGEAIRKLAEASDVITYEFENIDYDALNWLKEHAYLPQGSELLLLTQNRETEKKAIASAGCKVAPYR
IIHDRAELEASVAELGLPAVLKTCRGGYDGKGQHVLKEQAQIGEAAALLQHGTCILESWVPFKMELSVIVTRSVHGETTV
FPAAENIHKQNILFQSIVPARVDEAIQEQAAALALRLAEKLQLVGTLAVELFLKEDGGLLVNELAPRPHNSGHYTLDLCE
TSQFEQHIRAVCGLPLGKTDLLKEGMMVNLLGDEVYMAEEDPELLKEAKLYIYGKHEVKPGRKMGHITFLRTPDNEWIGQ
MTEKWIERDGGRKQ

Sequences:

>Translated_414_residues
MRRRFCQLLTKSLPKDWTNEGKASDKPCWKAVISLYKQTIFPGATIGIIGGGQLGKMMAVAAKQMGYKIAVVDPVPSSPC
GQVADLEITASYNDGEAIRKLAEASDVITYEFENIDYDALNWLKEHAYLPQGSELLLLTQNRETEKKAIASAGCKVAPYR
IIHDRAELEASVAELGLPAVLKTCRGGYDGKGQHVLKEQAQIGEAAALLQHGTCILESWVPFKMELSVIVTRSVHGETTV
FPAAENIHKQNILFQSIVPARVDEAIQEQAAALALRLAEKLQLVGTLAVELFLKEDGGLLVNELAPRPHNSGHYTLDLCE
TSQFEQHIRAVCGLPLGKTDLLKEGMMVNLLGDEVYMAEEDPELLKEAKLYIYGKHEVKPGRKMGHITFLRTPDNEWIGQ
MTEKWIERDGGRKQ
>Mature_414_residues
MRRRFCQLLTKSLPKDWTNEGKASDKPCWKAVISLYKQTIFPGATIGIIGGGQLGKMMAVAAKQMGYKIAVVDPVPSSPC
GQVADLEITASYNDGEAIRKLAEASDVITYEFENIDYDALNWLKEHAYLPQGSELLLLTQNRETEKKAIASAGCKVAPYR
IIHDRAELEASVAELGLPAVLKTCRGGYDGKGQHVLKEQAQIGEAAALLQHGTCILESWVPFKMELSVIVTRSVHGETTV
FPAAENIHKQNILFQSIVPARVDEAIQEQAAALALRLAEKLQLVGTLAVELFLKEDGGLLVNELAPRPHNSGHYTLDLCE
TSQFEQHIRAVCGLPLGKTDLLKEGMMVNLLGDEVYMAEEDPELLKEAKLYIYGKHEVKPGRKMGHITFLRTPDNEWIGQ
MTEKWIERDGGRKQ

Specific function: Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR) [H]

COG id: COG0026

COG function: function code F; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786733, Length=346, Percent_Identity=33.5260115606936, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1788155, Length=375, Percent_Identity=26.4, Blast_Score=107, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6324702, Length=364, Percent_Identity=36.8131868131868, Blast_Score=221, Evalue=1e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006115
- InterPro:   IPR005875
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp; PF03446 NAD_binding_2 [H]

EC number: =6.3.4.18 [H]

Molecular weight: Translated: 45803; Mature: 45803

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRRFCQLLTKSLPKDWTNEGKASDKPCWKAVISLYKQTIFPGATIGIIGGGQLGKMMAV
CCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHH
AAKQMGYKIAVVDPVPSSPCGQVADLEITASYNDGEAIRKLAEASDVITYEFENIDYDAL
HHHHHCCEEEEECCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHCCEEEEEECCCCHHHH
NWLKEHAYLPQGSELLLLTQNRETEKKAIASAGCKVAPYRIIHDRAELEASVAELGLPAV
HHHHHHCCCCCCCEEEEEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHH
LKTCRGGYDGKGQHVLKEQAQIGEAAALLQHGTCILESWVPFKMELSVIVTRSVHGETTV
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEEEEEEEEECCCCCEE
FPAAENIHKQNILFQSIVPARVDEAIQEQAAALALRLAEKLQLVGTLAVELFLKEDGGLL
ECCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEE
VNELAPRPHNSGHYTLDLCETSQFEQHIRAVCGLPLGKTDLLKEGMMVNLLGDEVYMAEE
EECCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCHHHHHHCCCEEEEECCEEEECCC
DPELLKEAKLYIYGKHEVKPGRKMGHITFLRTPDNEWIGQMTEKWIERDGGRKQ
CHHHHHCCEEEEEECCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRRRFCQLLTKSLPKDWTNEGKASDKPCWKAVISLYKQTIFPGATIGIIGGGQLGKMMAV
CCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHH
AAKQMGYKIAVVDPVPSSPCGQVADLEITASYNDGEAIRKLAEASDVITYEFENIDYDAL
HHHHHCCEEEEECCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHCCEEEEEECCCCHHHH
NWLKEHAYLPQGSELLLLTQNRETEKKAIASAGCKVAPYRIIHDRAELEASVAELGLPAV
HHHHHHCCCCCCCEEEEEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHH
LKTCRGGYDGKGQHVLKEQAQIGEAAALLQHGTCILESWVPFKMELSVIVTRSVHGETTV
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEEEEEEEEECCCCCEE
FPAAENIHKQNILFQSIVPARVDEAIQEQAAALALRLAEKLQLVGTLAVELFLKEDGGLL
ECCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEE
VNELAPRPHNSGHYTLDLCETSQFEQHIRAVCGLPLGKTDLLKEGMMVNLLGDEVYMAEE
EECCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCHHHHHHCCCEEEEECCEEEECCC
DPELLKEAKLYIYGKHEVKPGRKMGHITFLRTPDNEWIGQMTEKWIERDGGRKQ
CHHHHHCCEEEEEECCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377 [H]