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The map label for this gene is murF [H]

Identifier: 52784364

GI number: 52784364

Start: 549655

End: 551130

Strand: Direct

Name: murF [H]

Synonym: BLi00544

Alternate gene names: 52784364

Gene position: 549655-551130 (Clockwise)

Preceding gene: 52784363

Following gene: 52784365

Centisome position: 13.02

GC content: 48.85

Gene sequence:

>1476_bases
ATGGAGAAAGGCGCTGAAGGCGGTTCGCCCTTAGATACAGCTGCAGACACTATCCTTATTTGTGGTAGTGTCTCTTTTCC
TTGCAGAAGAAAGAGAGGGACATACATGATAACAAGAACGGTAAAAGAAATTGCTGAAATGTCGGAAGGCGTTTTGACGA
ATGAAGCTTTTGAAGGGCGTCTCATCAAAGGCGTCTCAACCGATTCGAGAAAACTGGCGAAAGGCCAACTGTTTATTCCG
CTTGCAGGCGAGCGGTTCAACGGACACGTTTTTGCAGAGCAGGCTTTTCAGGAAGGCGTTGCGGCCGTGCTGTGGAACCG
AAGTGAACCGAATCCTCCGGAAGGGAAGCCGGTCATTTTAGTTGACGATACGCTGGAAGCGCTGCAGAAGCTTGCGAAGG
CATACAGGAATCAGCTGGACGTAAAAGTCGTCGGCGTGACCGGTTCAAACGGAAAAACGACGACAAAAGACATGATTCAT
GCTGTTTTGGGCACTGAGTATAAAGTGCATAAGACAGAAGGAAACTACAATAACCATATCGGCCTGCCCCTGACGATTCT
GGCGATGCCCGAAGACACAGAAGTCGCCATTTTGGAAATGGGGATGAGCGCCAAAGGAGAAATCGATTTTCTCACTCGTC
TGGCGCGGCCTGATATTGCAGTCATTACAAACATTGGTGAATCACACCTCCTTGAACTCGGATCAAGAGAAGGAATTGCA
GAGGCGAAGCTCGAAATTGTCAATGGGCTTTCAAAAGAAGGCACACTCATTTATGTCGGAGATGAACCGCTGCTCACCGA
AAGGCTGAACCAGCCGCCTTACGATACAAAGACGTTTGGAGAGGATCCGGCTTTCGATTATCAGCTTGAGGATATGACAC
AGACTGAAGAGGGGACGGCATTCCGCGTGAGAGGAATCGAAAACGGGTTCTTCATCCCGGTCCTTGGAAAGCATAATGTG
AAAAACGCATTAGCCGCGATTGCGGCCGGAGACCTCTTGAATGTCAGCCGCGCTCACATTGCAGAAGGGCTGACTAAACT
GAAAGTGACCGGAATGCGGCTTGAGCTAGTAAAGACGCCGGACGGCGTTGCGATCATTAATGACGCCTATAATGCCAGCC
CGACATCGATGAAAGCGGCGATCGAGCTTGTCGAACAGATGAAGGGATACGGCAAGAAGATTCTTGTTCTCGGCGATATG
CTGGAGCTCGGAGAAGGAGAAAAAGCGTTTCATGAAGAAATCGGGCAGGCGGTCAACCCGGACTCGATCGACTTTTTATT
TACGTATGGAGAACTCGGTGCATATATTGCAAAAGGGGCGCTTAAACATTTCCCGGAACACCGCGTCCTTCATTTCAACC
CGGAAGAAAAAGACAGTTTGAAAAAGACACTGAAAGAAAAAGCGAATACAGGGGATTTAATCCTCGTCAAAGCGTCCAGA
GGCATGCGCCTTGAGGATGTTATAAAAGATTTGTAA

Upstream 100 bases:

>100_bases
TTGAAAAGCTTGTCGCTTTGGCAATCGAGCGCCACGAAGAAAAACAGCAGATTAAACATACATTTTAACGGGACGGAGCG
GACCGAATCCGGGAGCTTTC

Downstream 100 bases:

>100_bases
GGGAACTTTGAATCATAAAGTCGTTTTTTATGTGATGATCAGTAAAAGAAAACGGGTGAATGGTCATGATAGGATGTTTA
TGCATTCACGGATTTACCGG

Product: hypothetical protein

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MEKGAEGGSPLDTAADTILICGSVSFPCRRKRGTYMITRTVKEIAEMSEGVLTNEAFEGRLIKGVSTDSRKLAKGQLFIP
LAGERFNGHVFAEQAFQEGVAAVLWNRSEPNPPEGKPVILVDDTLEALQKLAKAYRNQLDVKVVGVTGSNGKTTTKDMIH
AVLGTEYKVHKTEGNYNNHIGLPLTILAMPEDTEVAILEMGMSAKGEIDFLTRLARPDIAVITNIGESHLLELGSREGIA
EAKLEIVNGLSKEGTLIYVGDEPLLTERLNQPPYDTKTFGEDPAFDYQLEDMTQTEEGTAFRVRGIENGFFIPVLGKHNV
KNALAAIAAGDLLNVSRAHIAEGLTKLKVTGMRLELVKTPDGVAIINDAYNASPTSMKAAIELVEQMKGYGKKILVLGDM
LELGEGEKAFHEEIGQAVNPDSIDFLFTYGELGAYIAKGALKHFPEHRVLHFNPEEKDSLKKTLKEKANTGDLILVKASR
GMRLEDVIKDL

Sequences:

>Translated_491_residues
MEKGAEGGSPLDTAADTILICGSVSFPCRRKRGTYMITRTVKEIAEMSEGVLTNEAFEGRLIKGVSTDSRKLAKGQLFIP
LAGERFNGHVFAEQAFQEGVAAVLWNRSEPNPPEGKPVILVDDTLEALQKLAKAYRNQLDVKVVGVTGSNGKTTTKDMIH
AVLGTEYKVHKTEGNYNNHIGLPLTILAMPEDTEVAILEMGMSAKGEIDFLTRLARPDIAVITNIGESHLLELGSREGIA
EAKLEIVNGLSKEGTLIYVGDEPLLTERLNQPPYDTKTFGEDPAFDYQLEDMTQTEEGTAFRVRGIENGFFIPVLGKHNV
KNALAAIAAGDLLNVSRAHIAEGLTKLKVTGMRLELVKTPDGVAIINDAYNASPTSMKAAIELVEQMKGYGKKILVLGDM
LELGEGEKAFHEEIGQAVNPDSIDFLFTYGELGAYIAKGALKHFPEHRVLHFNPEEKDSLKKTLKEKANTGDLILVKASR
GMRLEDVIKDL
>Mature_491_residues
MEKGAEGGSPLDTAADTILICGSVSFPCRRKRGTYMITRTVKEIAEMSEGVLTNEAFEGRLIKGVSTDSRKLAKGQLFIP
LAGERFNGHVFAEQAFQEGVAAVLWNRSEPNPPEGKPVILVDDTLEALQKLAKAYRNQLDVKVVGVTGSNGKTTTKDMIH
AVLGTEYKVHKTEGNYNNHIGLPLTILAMPEDTEVAILEMGMSAKGEIDFLTRLARPDIAVITNIGESHLLELGSREGIA
EAKLEIVNGLSKEGTLIYVGDEPLLTERLNQPPYDTKTFGEDPAFDYQLEDMTQTEEGTAFRVRGIENGFFIPVLGKHNV
KNALAAIAAGDLLNVSRAHIAEGLTKLKVTGMRLELVKTPDGVAIINDAYNASPTSMKAAIELVEQMKGYGKKILVLGDM
LELGEGEKAFHEEIGQAVNPDSIDFLFTYGELGAYIAKGALKHFPEHRVLHFNPEEKDSLKKTLKEKANTGDLILVKASR
GMRLEDVIKDL

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=466, Percent_Identity=33.4763948497854, Blast_Score=202, Evalue=5e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 53572; Mature: 53572

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKGAEGGSPLDTAADTILICGSVSFPCRRKRGTYMITRTVKEIAEMSEGVLTNEAFEGR
CCCCCCCCCCCHHCCCEEEEECCCCCCCHHCCCCEEEHHHHHHHHHHHCCCCCCCCCCCE
LIKGVSTDSRKLAKGQLFIPLAGERFNGHVFAEQAFQEGVAAVLWNRSEPNPPEGKPVIL
EEECCCCCCHHHCCCEEEEEECCCCCCCEEEHHHHHHCCCEEEEECCCCCCCCCCCEEEE
VDDTLEALQKLAKAYRNQLDVKVVGVTGSNGKTTTKDMIHAVLGTEYKVHKTEGNYNNHI
ECHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCC
GLPLTILAMPEDTEVAILEMGMSAKGEIDFLTRLARPDIAVITNIGESHLLELGSREGIA
CCCEEEEECCCCCCEEEEEECCCCCCCHHHHHHHCCCCEEEEECCCHHHHHHCCCCCCHH
EAKLEIVNGLSKEGTLIYVGDEPLLTERLNQPPYDTKTFGEDPAFDYQLEDMTQTEEGTA
HHHHHHHCCCCCCCEEEEECCCCHHHHHCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCE
FRVRGIENGFFIPVLGKHNVKNALAAIAAGDLLNVSRAHIAEGLTKLKVTGMRLELVKTP
EEEEECCCCEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHCHHHEEEECEEEEEEECC
DGVAIINDAYNASPTSMKAAIELVEQMKGYGKKILVLGDMLELGEGEKAFHEEIGQAVNP
CCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECHHHCCCCCHHHHHHHCCCCCC
DSIDFLFTYGELGAYIAKGALKHFPEHRVLHFNPEEKDSLKKTLKEKANTGDLILVKASR
CCEEEEEEHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCCCCEEEEECCC
GMRLEDVIKDL
CCCHHHHHHCC
>Mature Secondary Structure
MEKGAEGGSPLDTAADTILICGSVSFPCRRKRGTYMITRTVKEIAEMSEGVLTNEAFEGR
CCCCCCCCCCCHHCCCEEEEECCCCCCCHHCCCCEEEHHHHHHHHHHHCCCCCCCCCCCE
LIKGVSTDSRKLAKGQLFIPLAGERFNGHVFAEQAFQEGVAAVLWNRSEPNPPEGKPVIL
EEECCCCCCHHHCCCEEEEEECCCCCCCEEEHHHHHHCCCEEEEECCCCCCCCCCCEEEE
VDDTLEALQKLAKAYRNQLDVKVVGVTGSNGKTTTKDMIHAVLGTEYKVHKTEGNYNNHI
ECHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCC
GLPLTILAMPEDTEVAILEMGMSAKGEIDFLTRLARPDIAVITNIGESHLLELGSREGIA
CCCEEEEECCCCCCEEEEEECCCCCCCHHHHHHHCCCCEEEEECCCHHHHHHCCCCCCHH
EAKLEIVNGLSKEGTLIYVGDEPLLTERLNQPPYDTKTFGEDPAFDYQLEDMTQTEEGTA
HHHHHHHCCCCCCCEEEEECCCCHHHHHCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCE
FRVRGIENGFFIPVLGKHNVKNALAAIAAGDLLNVSRAHIAEGLTKLKVTGMRLELVKTP
EEEEECCCCEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHCHHHEEEECEEEEEEECC
DGVAIINDAYNASPTSMKAAIELVEQMKGYGKKILVLGDMLELGEGEKAFHEEIGQAVNP
CCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECHHHCCCCCHHHHHHHCCCCCC
DSIDFLFTYGELGAYIAKGALKHFPEHRVLHFNPEEKDSLKKTLKEKANTGDLILVKASR
CCEEEEEEHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCCCCEEEEECCC
GMRLEDVIKDL
CCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]