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The map label for this gene is ddl

Identifier: 52784363

GI number: 52784363

Start: 548537

End: 549622

Strand: Direct

Name: ddl

Synonym: BLi00543

Alternate gene names: 52784363

Gene position: 548537-549622 (Clockwise)

Preceding gene: 52784360

Following gene: 52784364

Centisome position: 12.99

GC content: 46.87

Gene sequence:

>1086_bases
TTGAAGACAAGATTAGGATTGGTATACGGCGGAAAATCAGCGGAGCATAACGTTTCTCTGCAAACCGCGCTGGCCGTCAC
GAAAGCATTAGATACTGAAAAATTTGATATACACCCGATTTATATAACAGAAAAAGGCGAGTGGGTGAGAGGGCCGCAAC
TGACAGAACCGGTTTCAAATGTGAAAATGCTGCAATTTGAACAGACGGGCCAGACATTTTCTCCGGCTGTGTTGAACCGC
GATATGTTTCCTGGCGAAGCTGATGCAAAAGAAGACTCTATCGATGTTGTTTTCCCGCTTTTGCACGGACCGAATGGGGA
AGACGGCACCATTCAAGGGATGCTTGAACTATTGAATGTGCCATATGTGGGCAACGGAGTCTTGGCTTCCTCTGCGGGCA
TGGATAAAGTGGTCATGAAGCATCTGTTTGCACAGGCAGGTCTTGACCAGGCGAAATACGTTTCATTTTTGAAAAAAACT
TGGAGTCAGTCTAAAGAAGAATGCTATGCTCAAGTGGAAGGGGAACTTGGCTATCCGTGCTTTGTAAAACCGGCCAACCT
TGGATCAAGCGTCGGGATCAGCAAATGCAGAAGCCGTGAAGAGCTTGACCAAGCCTTTGAGCTTGCATTCCAATATGACC
GGAAAATTGTCGTTGAAGAGGGCGTCATCGGACGGGAAATAGAACTCGGCGTTTTAGGGAATGATGAACCTGTCTGCTCA
GTCGCGGGGGAAATCGCTCCGAAGAAAGACTTTTATGACTATAAAGCAAAATATGAAGACGGCGATACTGATTTGATTAT
TCCGGCTTCCCTGACAGAAGACGAATACGAAACAATGCGCAGCATGGCGGTCAAAGCGTTTCAGGCGATTGACGGTTCAG
GCCTTGTGAGAGCGGACTTTTTCCTTACAAATGAAGGCAGGGTCCTGATCAATGAAGTCAATACGATGCCGGGATTCACC
CCGTTCAGCATGTTTCCGCTTCTCTGGAAGCAATCAGGGGTTGAGTACGCTGAACTGATTGAAAAGCTTGTCGCTTTGGC
AATCGAGCGCCACGAAGAAAAACAGCAGATTAAACATACATTTTAA

Upstream 100 bases:

>100_bases
GCTTTTTATATGCTGTTTTTAGACCGTATTAAAATAATTAGATCATTTTCCCGGGAAAGTGATAAGATAGTAAAGATGAA
TCCAGTGGGGGGAGTACGAA

Downstream 100 bases:

>100_bases
CGGGACGGAGCGGACCGAATCCGGGAGCTTTCATGGAGAAAGGCGCTGAAGGCGGTTCGCCCTTAGATACAGCTGCAGAC
ACTATCCTTATTTGTGGTAG

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MKTRLGLVYGGKSAEHNVSLQTALAVTKALDTEKFDIHPIYITEKGEWVRGPQLTEPVSNVKMLQFEQTGQTFSPAVLNR
DMFPGEADAKEDSIDVVFPLLHGPNGEDGTIQGMLELLNVPYVGNGVLASSAGMDKVVMKHLFAQAGLDQAKYVSFLKKT
WSQSKEECYAQVEGELGYPCFVKPANLGSSVGISKCRSREELDQAFELAFQYDRKIVVEEGVIGREIELGVLGNDEPVCS
VAGEIAPKKDFYDYKAKYEDGDTDLIIPASLTEDEYETMRSMAVKAFQAIDGSGLVRADFFLTNEGRVLINEVNTMPGFT
PFSMFPLLWKQSGVEYAELIEKLVALAIERHEEKQQIKHTF

Sequences:

>Translated_361_residues
MKTRLGLVYGGKSAEHNVSLQTALAVTKALDTEKFDIHPIYITEKGEWVRGPQLTEPVSNVKMLQFEQTGQTFSPAVLNR
DMFPGEADAKEDSIDVVFPLLHGPNGEDGTIQGMLELLNVPYVGNGVLASSAGMDKVVMKHLFAQAGLDQAKYVSFLKKT
WSQSKEECYAQVEGELGYPCFVKPANLGSSVGISKCRSREELDQAFELAFQYDRKIVVEEGVIGREIELGVLGNDEPVCS
VAGEIAPKKDFYDYKAKYEDGDTDLIIPASLTEDEYETMRSMAVKAFQAIDGSGLVRADFFLTNEGRVLINEVNTMPGFT
PFSMFPLLWKQSGVEYAELIEKLVALAIERHEEKQQIKHTF
>Mature_361_residues
MKTRLGLVYGGKSAEHNVSLQTALAVTKALDTEKFDIHPIYITEKGEWVRGPQLTEPVSNVKMLQFEQTGQTFSPAVLNR
DMFPGEADAKEDSIDVVFPLLHGPNGEDGTIQGMLELLNVPYVGNGVLASSAGMDKVVMKHLFAQAGLDQAKYVSFLKKT
WSQSKEECYAQVEGELGYPCFVKPANLGSSVGISKCRSREELDQAFELAFQYDRKIVVEEGVIGREIELGVLGNDEPVCS
VAGEIAPKKDFYDYKAKYEDGDTDLIIPASLTEDEYETMRSMAVKAFQAIDGSGLVRADFFLTNEGRVLINEVNTMPGFT
PFSMFPLLWKQSGVEYAELIEKLVALAIERHEEKQQIKHTF

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786579, Length=370, Percent_Identity=42.972972972973, Blast_Score=308, Evalue=4e-85,
Organism=Escherichia coli, GI1786280, Length=347, Percent_Identity=31.9884726224784, Blast_Score=181, Evalue=8e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_BACLD (Q65N65)

Other databases:

- EMBL:   CP000002
- EMBL:   AE017333
- RefSeq:   YP_077782.1
- RefSeq:   YP_090192.1
- HSSP:   P07862
- ProteinModelPortal:   Q65N65
- SMR:   Q65N65
- STRING:   Q65N65
- EnsemblBacteria:   EBBACT00000054747
- EnsemblBacteria:   EBBACT00000062397
- GeneID:   3030394
- GeneID:   3099263
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi00543
- KEGG:   bli:BL02193
- NMPDR:   fig|279010.5.peg.679
- eggNOG:   COG1181
- GeneTree:   EBGT00050000001406
- HOGENOM:   HBG427092
- OMA:   FTDISMY
- ProtClustDB:   PRK01966
- BioCyc:   BLIC279010-1:BLI00543-MONOMER
- BioCyc:   BLIC279010:BL02193-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 40057; Mature: 40057

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTRLGLVYGGKSAEHNVSLQTALAVTKALDTEKFDIHPIYITEKGEWVRGPQLTEPVSN
CCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCHHC
VKMLQFEQTGQTFSPAVLNRDMFPGEADAKEDSIDVVFPLLHGPNGEDGTIQGMLELLNV
CEEEEEECCCCCCCHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHCC
PYVGNGVLASSAGMDKVVMKHLFAQAGLDQAKYVSFLKKTWSQSKEECYAQVEGELGYPC
CCCCCCCEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCE
FVKPANLGSSVGISKCRSREELDQAFELAFQYDRKIVVEEGVIGREIELGVLGNDEPVCS
EEECCCCCCCCCHHHHCCHHHHHHHHHHHHHHCCEEEEECCCCCCEEEEEEECCCCCHHH
VAGEIAPKKDFYDYKAKYEDGDTDLIIPASLTEDEYETMRSMAVKAFQAIDGSGLVRADF
HHCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEE
FLTNEGRVLINEVNTMPGFTPFSMFPLLWKQSGVEYAELIEKLVALAIERHEEKQQIKHT
EEECCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
F
C
>Mature Secondary Structure
MKTRLGLVYGGKSAEHNVSLQTALAVTKALDTEKFDIHPIYITEKGEWVRGPQLTEPVSN
CCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCHHC
VKMLQFEQTGQTFSPAVLNRDMFPGEADAKEDSIDVVFPLLHGPNGEDGTIQGMLELLNV
CEEEEEECCCCCCCHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHCC
PYVGNGVLASSAGMDKVVMKHLFAQAGLDQAKYVSFLKKTWSQSKEECYAQVEGELGYPC
CCCCCCCEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCE
FVKPANLGSSVGISKCRSREELDQAFELAFQYDRKIVVEEGVIGREIELGVLGNDEPVCS
EEECCCCCCCCCHHHHCCHHHHHHHHHHHHHHCCEEEEECCCCCCEEEEEEECCCCCHHH
VAGEIAPKKDFYDYKAKYEDGDTDLIIPASLTEDEYETMRSMAVKAFQAIDGSGLVRADF
HHCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEE
FLTNEGRVLINEVNTMPGFTPFSMFPLLWKQSGVEYAELIEKLVALAIERHEEKQQIKHT
EEECCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
F
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA