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The map label for this gene is topB [H]

Identifier: 52784338

GI number: 52784338

Start: 522521

End: 524713

Strand: Direct

Name: topB [H]

Synonym: BLi00518

Alternate gene names: 52784338

Gene position: 522521-524713 (Clockwise)

Preceding gene: 52784337

Following gene: 52784339

Centisome position: 12.37

GC content: 50.39

Gene sequence:

>2193_bases
ATGATGGACGTGTCTAAAACAGTTGTTTTAGCAGAAAAACCTTCAGTCGGCCGTGACTTGGCCCGCGTGCTGAAGTGTCA
TAAAAAAGGAAACGGATATCTTGAAGGCGACCGCTATATCGTGACATGGGCGCTCGGCCATCTGGTCACATTGGCGGACC
CTGAAGGATACGGAAAGGAATACCAGTCTTGGCGGCTTGAAGACCTGCCGATCATCCCTGAGCCTTTAAAGCTCGTCGTG
ATCAAAAAGACCGGAAAACAGTTTCAGGCGGTAAAATCGCAGCTGATCAGAAAAGATGTCAAAGACATCGTCATTGCAAC
CGATGCCGGCCGGGAAGGCGAGCTCGTCGCCCGCTGGATCATCGAAAAAGCAAAGGTCACGAAGCCATTGAAGCGTCTGT
GGATTTCATCTGTTACTGACAAAGCGATCAAAGACGGATTTAAAAACTTAAAGAACGGCAAAGACTTTGAAAACCTTTAC
CATTCAGCTGTGGCTCGAGCTGAAGCCGATTGGATCGTCGGCATCAACGCGACGAGGGCGCTGACAACGAAATTCAACGC
CCAGCTTTCCTGCGGGCGCGTACAGACGCCGACGCTGGCAATGATCGCCAAAAGAGAGGAAGATATTAAGAACTTTAAAC
CCGTCCCTTATTTCGGCTTGCGCGCAGCGGTCGACGGCATGACGCTGACATGGCAGGACAAAAAGACGTCACAGACACGC
ACGTTCAACGCATCTGTGACGTCGCAGCTTGCAGGAGCGCTGCAAGGCAAACCGGCCGTCATCGTCGACTTGAAAAAAAC
GGCGAAAAAAAGCTTTGCTCCCGGACTTTATGATCTGACTGAGCTGCAGAGGGACGCCCATAAACGCTTCGGCTTTTCCG
CAAAAGAAACGCTGTCCGTTTTGCAGAAGCTCTATGAGCAGCACAAGCTCGTCACCTATCCGCGGACGGACTCAAGGTTT
CTTTCCAATGACATCGTCCCGACGCTGAAAGACCGTTTGGAAGGCATGCAGGTGAAGCCTTATGCCCAGCATGTGGCCCG
GATCCTGAAGCGGGGGGTTAAAGCCAATAAAAGCTTTGTCAACGATGCGAAAGTATCCGATCACCACGCGATTATTCCGA
CGGAAGAGCCGCTTGCGCTCGGCGCGTTAAGCGAAAAGGAAAGAAAGCTTTACGATTTGATCGCGAAGCGCTTCCTCGCT
GTTTTAATGCCGCCGTTTGAATATGAAGAAACAAAAGTGTTTGCCGAAATCGGCGGAGAAACCTTTACGGCAAAAGGGAA
AACAGTTCAGTCGCAAGGCTGGAAAGCGGTGTACGATTTCGCAGACGAAGATGATGACGAGGAAGAAAAGGATCAAACCC
TTCCAAAACTCGCAAAAGGCGACACGCTTTCCGTCCGGTCTTTGACAGAGACGAAGGGTGAAACAAAACCGCCGGCGCGG
TTTAATGAAGGAACATTGCTGTCTGCGATGGAGAATCCAGCCGCATTTATGCAGGGAGAAGAAAAAAACCTTGTGAAAAC
CCTCGGAGAGACGGGCGGACTTGGGACGGTCGCGACACGCGCCGATATTATCGAAAAGCTGTTCAACACGTTCCTGATTG
AGAAAAAAGGAAAAGATATTTACATTACTTCAAAAGGAAAACAGCTCCTTGAGCTTGTTCCGTCAGATTTAAAATCTCCG
GCCCTCACCGCCGAATGGGAGCAGAAGCTGTCAGGCATTGCGAAAGGAAAGCTGAAATCTTCAGCCTTCATTAAAGAAAT
GAAGGAGTACGCAAAGCAGACGATCAGAGAAATCAAAAGCAGCAATCAAAAGTTTAAGCACGACAATATCACAGGAACAC
ATTGCCCTGACTGCGGAAAACTGATGCTGAAAGTAAACGGCAAACGCGGCACAATGCTCGTCTGCCAGGACAGGGAGTGC
GGCCATCGGAAAACGGTCGCCAAACAGACGAACGCCCGCTGCCCGGTTTGCCATAAGCGCATGGAGCTGCGCGGCCACGG
GGAAGGCCAAACCTTTGCCTGTGTCTGCGGCCACCGCGAAAAGCTCTCCGTTTTCGAAAAGCGCAGAGCCAAAGACAAAA
ATTCAAAAGCGTCAAAACGCGATGTACACTCTTACATGAAAAAGCAAAACAAGGATGAGCCGATCAACAATGCGCTCGCC
GAACAGCTGAAGAAGCTGAAGCTTGATCAATAA

Upstream 100 bases:

>100_bases
GTGATTTTTTCGGAAATTGAAACAAAGGAATCGTGAACATTAGAGATGCATCTGTATCATCTGTTATAATATAAGGATGC
ATGAACAAGAAAGTGATGTG

Downstream 100 bases:

>100_bases
AACGGGAAGCCTGCCGGGGACGGCAGGCTGTTTTAATTTGTCCAAATATCACAAAAAGCTGCTGTTATGACGCGATGATA
GAAGGAGATTCACTGCGCGG

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 730; Mature: 730

Protein sequence:

>730_residues
MMDVSKTVVLAEKPSVGRDLARVLKCHKKGNGYLEGDRYIVTWALGHLVTLADPEGYGKEYQSWRLEDLPIIPEPLKLVV
IKKTGKQFQAVKSQLIRKDVKDIVIATDAGREGELVARWIIEKAKVTKPLKRLWISSVTDKAIKDGFKNLKNGKDFENLY
HSAVARAEADWIVGINATRALTTKFNAQLSCGRVQTPTLAMIAKREEDIKNFKPVPYFGLRAAVDGMTLTWQDKKTSQTR
TFNASVTSQLAGALQGKPAVIVDLKKTAKKSFAPGLYDLTELQRDAHKRFGFSAKETLSVLQKLYEQHKLVTYPRTDSRF
LSNDIVPTLKDRLEGMQVKPYAQHVARILKRGVKANKSFVNDAKVSDHHAIIPTEEPLALGALSEKERKLYDLIAKRFLA
VLMPPFEYEETKVFAEIGGETFTAKGKTVQSQGWKAVYDFADEDDDEEEKDQTLPKLAKGDTLSVRSLTETKGETKPPAR
FNEGTLLSAMENPAAFMQGEEKNLVKTLGETGGLGTVATRADIIEKLFNTFLIEKKGKDIYITSKGKQLLELVPSDLKSP
ALTAEWEQKLSGIAKGKLKSSAFIKEMKEYAKQTIREIKSSNQKFKHDNITGTHCPDCGKLMLKVNGKRGTMLVCQDREC
GHRKTVAKQTNARCPVCHKRMELRGHGEGQTFACVCGHREKLSVFEKRRAKDKNSKASKRDVHSYMKKQNKDEPINNALA
EQLKKLKLDQ

Sequences:

>Translated_730_residues
MMDVSKTVVLAEKPSVGRDLARVLKCHKKGNGYLEGDRYIVTWALGHLVTLADPEGYGKEYQSWRLEDLPIIPEPLKLVV
IKKTGKQFQAVKSQLIRKDVKDIVIATDAGREGELVARWIIEKAKVTKPLKRLWISSVTDKAIKDGFKNLKNGKDFENLY
HSAVARAEADWIVGINATRALTTKFNAQLSCGRVQTPTLAMIAKREEDIKNFKPVPYFGLRAAVDGMTLTWQDKKTSQTR
TFNASVTSQLAGALQGKPAVIVDLKKTAKKSFAPGLYDLTELQRDAHKRFGFSAKETLSVLQKLYEQHKLVTYPRTDSRF
LSNDIVPTLKDRLEGMQVKPYAQHVARILKRGVKANKSFVNDAKVSDHHAIIPTEEPLALGALSEKERKLYDLIAKRFLA
VLMPPFEYEETKVFAEIGGETFTAKGKTVQSQGWKAVYDFADEDDDEEEKDQTLPKLAKGDTLSVRSLTETKGETKPPAR
FNEGTLLSAMENPAAFMQGEEKNLVKTLGETGGLGTVATRADIIEKLFNTFLIEKKGKDIYITSKGKQLLELVPSDLKSP
ALTAEWEQKLSGIAKGKLKSSAFIKEMKEYAKQTIREIKSSNQKFKHDNITGTHCPDCGKLMLKVNGKRGTMLVCQDREC
GHRKTVAKQTNARCPVCHKRMELRGHGEGQTFACVCGHREKLSVFEKRRAKDKNSKASKRDVHSYMKKQNKDEPINNALA
EQLKKLKLDQ
>Mature_730_residues
MMDVSKTVVLAEKPSVGRDLARVLKCHKKGNGYLEGDRYIVTWALGHLVTLADPEGYGKEYQSWRLEDLPIIPEPLKLVV
IKKTGKQFQAVKSQLIRKDVKDIVIATDAGREGELVARWIIEKAKVTKPLKRLWISSVTDKAIKDGFKNLKNGKDFENLY
HSAVARAEADWIVGINATRALTTKFNAQLSCGRVQTPTLAMIAKREEDIKNFKPVPYFGLRAAVDGMTLTWQDKKTSQTR
TFNASVTSQLAGALQGKPAVIVDLKKTAKKSFAPGLYDLTELQRDAHKRFGFSAKETLSVLQKLYEQHKLVTYPRTDSRF
LSNDIVPTLKDRLEGMQVKPYAQHVARILKRGVKANKSFVNDAKVSDHHAIIPTEEPLALGALSEKERKLYDLIAKRFLA
VLMPPFEYEETKVFAEIGGETFTAKGKTVQSQGWKAVYDFADEDDDEEEKDQTLPKLAKGDTLSVRSLTETKGETKPPAR
FNEGTLLSAMENPAAFMQGEEKNLVKTLGETGGLGTVATRADIIEKLFNTFLIEKKGKDIYITSKGKQLLELVPSDLKSP
ALTAEWEQKLSGIAKGKLKSSAFIKEMKEYAKQTIREIKSSNQKFKHDNITGTHCPDCGKLMLKVNGKRGTMLVCQDREC
GHRKTVAKQTNARCPVCHKRMELRGHGEGQTFACVCGHREKLSVFEKRRAKDKNSKASKRDVHSYMKKQNKDEPINNALA
EQLKKLKLDQ

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=677, Percent_Identity=24.0768094534712, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI4507635, Length=622, Percent_Identity=24.91961414791, Blast_Score=127, Evalue=5e-29,
Organism=Escherichia coli, GI1788061, Length=620, Percent_Identity=34.1935483870968, Blast_Score=342, Evalue=7e-95,
Organism=Escherichia coli, GI1787529, Length=555, Percent_Identity=26.3063063063063, Blast_Score=145, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI32563869, Length=705, Percent_Identity=25.1063829787234, Blast_Score=143, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI17555378, Length=511, Percent_Identity=23.679060665362, Blast_Score=102, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6323263, Length=663, Percent_Identity=23.0769230769231, Blast_Score=106, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24585251, Length=583, Percent_Identity=23.8421955403087, Blast_Score=123, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24640096, Length=622, Percent_Identity=23.7942122186495, Blast_Score=112, Evalue=8e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 81850; Mature: 81850

Theoretical pI: Translated: 10.15; Mature: 10.15

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMDVSKTVVLAEKPSVGRDLARVLKCHKKGNGYLEGDRYIVTWALGHLVTLADPEGYGKE
CCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCEECCCCEEEEEEHHCEEEECCCCCCCCH
YQSWRLEDLPIIPEPLKLVVIKKTGKQFQAVKSQLIRKDVKDIVIATDAGREGELVARWI
HHCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHH
IEKAKVTKPLKRLWISSVTDKAIKDGFKNLKNGKDFENLYHSAVARAEADWIVGINATRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEECCCHHH
LTTKFNAQLSCGRVQTPTLAMIAKREEDIKNFKPVPYFGLRAAVDGMTLTWQDKKTSQTR
HHHHCCCCEECCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCEEEECCCCCCCCE
TFNASVTSQLAGALQGKPAVIVDLKKTAKKSFAPGLYDLTELQRDAHKRFGFSAKETLSV
EECHHHHHHHHHHHCCCCEEEEECHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHH
LQKLYEQHKLVTYPRTDSRFLSNDIVPTLKDRLEGMQVKPYAQHVARILKRGVKANKSFV
HHHHHHCCCEEECCCCCCHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHH
NDAKVSDHHAIIPTEEPLALGALSEKERKLYDLIAKRFLAVLMPPFEYEETKVFAEIGGE
CCCCCCCCEEECCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCC
TFTAKGKTVQSQGWKAVYDFADEDDDEEEKDQTLPKLAKGDTLSVRSLTETKGETKPPAR
EEECCCCEECCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEHHHHHCCCCCCCCCC
FNEGTLLSAMENPAAFMQGEEKNLVKTLGETGGLGTVATRADIIEKLFNTFLIEKKGKDI
CCCCCEEHHHCCCHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHEECCCCEE
YITSKGKQLLELVPSDLKSPALTAEWEQKLSGIAKGKLKSSAFIKEMKEYAKQTIREIKS
EEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SNQKFKHDNITGTHCPDCGKLMLKVNGKRGTMLVCQDRECGHRKTVAKQTNARCPVCHKR
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEECCCCCCCHHHHHHHCCCCCCHHHHH
MELRGHGEGQTFACVCGHREKLSVFEKRRAKDKNSKASKRDVHSYMKKQNKDEPINNALA
HHHCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHH
EQLKKLKLDQ
HHHHHHCCCC
>Mature Secondary Structure
MMDVSKTVVLAEKPSVGRDLARVLKCHKKGNGYLEGDRYIVTWALGHLVTLADPEGYGKE
CCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCEECCCCEEEEEEHHCEEEECCCCCCCCH
YQSWRLEDLPIIPEPLKLVVIKKTGKQFQAVKSQLIRKDVKDIVIATDAGREGELVARWI
HHCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHH
IEKAKVTKPLKRLWISSVTDKAIKDGFKNLKNGKDFENLYHSAVARAEADWIVGINATRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEECCCHHH
LTTKFNAQLSCGRVQTPTLAMIAKREEDIKNFKPVPYFGLRAAVDGMTLTWQDKKTSQTR
HHHHCCCCEECCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCEEEECCCCCCCCE
TFNASVTSQLAGALQGKPAVIVDLKKTAKKSFAPGLYDLTELQRDAHKRFGFSAKETLSV
EECHHHHHHHHHHHCCCCEEEEECHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHH
LQKLYEQHKLVTYPRTDSRFLSNDIVPTLKDRLEGMQVKPYAQHVARILKRGVKANKSFV
HHHHHHCCCEEECCCCCCHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHH
NDAKVSDHHAIIPTEEPLALGALSEKERKLYDLIAKRFLAVLMPPFEYEETKVFAEIGGE
CCCCCCCCEEECCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCC
TFTAKGKTVQSQGWKAVYDFADEDDDEEEKDQTLPKLAKGDTLSVRSLTETKGETKPPAR
EEECCCCEECCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEHHHHHCCCCCCCCCC
FNEGTLLSAMENPAAFMQGEEKNLVKTLGETGGLGTVATRADIIEKLFNTFLIEKKGKDI
CCCCCEEHHHCCCHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHEECCCCEE
YITSKGKQLLELVPSDLKSPALTAEWEQKLSGIAKGKLKSSAFIKEMKEYAKQTIREIKS
EEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SNQKFKHDNITGTHCPDCGKLMLKVNGKRGTMLVCQDRECGHRKTVAKQTNARCPVCHKR
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEECCCCCCCHHHHHHHCCCCCCHHHHH
MELRGHGEGQTFACVCGHREKLSVFEKRRAKDKNSKASKRDVHSYMKKQNKDEPINNALA
HHHCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHH
EQLKKLKLDQ
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA