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The map label for this gene is potD-A [H]

Identifier: 52784219

GI number: 52784219

Start: 374077

End: 375159

Strand: Direct

Name: potD-A [H]

Synonym: BLi00397

Alternate gene names: 52784219

Gene position: 374077-375159 (Clockwise)

Preceding gene: 52784218

Following gene: 52784220

Centisome position: 8.86

GC content: 47.37

Gene sequence:

>1083_bases
ATGAAAAGTAAAATTTCTGCTTTATTTATTCTGTCTCTTGCAGCGGTGCTCCTCCTTTCGGGATGCGGCGGAAACGGAAG
TCAGGAAAGCAAACAATCAGATACAGTCGTTATCGGCGTCTATGGGGGCGATTGGGAAAAACATATCAAGCCGAGCCTCG
AATCTTTTGAAAAAGAAACAGGACTGAAGGTCAAAGTTGTCGCAGGGACAGACTCTGAGTGGTTCACAAAACTCAAGGCA
TCGAATGGCAGAAATGCTCCCTATGATCTCTTAATTCTGCAGCCGGACACGATTCACAGAGCGCAGTCGGCGGGATTGCT
GGCACCGATCGATCAGGAAAAGGTTCCGAATGTCCGCGATCTGTACCACTCTGTTCAGAAACGCTTCACCGATGATGGAA
AGCAGTATGCGGCCGGTTTCAGTATGGGACAGCTTGGAATTGTCTACCGAAAGGATCTTGTACCACAGGAGCCTAAGCGC
TGGACGGATCTTTGGAACAGCAGCTATAAAGGTCATGTCGCCATATCATCGCCGGCTTATTCGGCGGGGCTTCAGTTTTT
TGTAGGATTAATCAATGCCCAGGGCGGCCTGGAGTCAAACGGGAAAGACGTGGATAAAGCGTTCAACAGCTTGGCTGATT
TAAAGAACAACATAACGGCCTATCCCGATAATCCAGGCTCGATACAAACGCTTCTGGAACGTGGGGATGCATGGGTTGTT
CCGTTTTGGGACGGCCGCGCTTTTTCTTTGCAGGATGCAGGTCTTGACCTGGGATTTGCATATCCCGAGGAAGGTGCAGT
CGCAGCAGTTGCAAGCTGGGCTTTAATGAAAGGAAGTCCTAATCAGGATAATGCGTACAAGCTGTTAAATCATCTGCTCA
GTCCAGAAGTGCAAGGAGATTTTGCCGATAGAACGTTTTACGGCATGGCCAATAAAAAAGTGAAATACAGCGATAAATTA
AAAGGGAAGATCAAAGTAGGGGAGGATTATTACAGGAATTTAAAATGGGTTGACTATGAAGTCGCGGCAAAAGATCTAGG
CGATTGGACAAATCGCTGGAATCAAGTACTTGGCGGAAAATAG

Upstream 100 bases:

>100_bases
ATTTCTCAAAAGAACATAACGGTCCGATCAGTAAAAGCGAAGTTGCGGCCGCTGTGGAAAAACTGGCTGGAAAAAATAAC
ACGGGAATGGTGAGATGACA

Downstream 100 bases:

>100_bases
GTGACAAACATGAGCGCAATTCAGATTAGGCATGTCAGCCGGCATTTCGGAAACACCATCGCATTGGAAGACGTACAGCT
TGAAGTCAAAGAAGGGGAGT

Product: hypothetical protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MKSKISALFILSLAAVLLLSGCGGNGSQESKQSDTVVIGVYGGDWEKHIKPSLESFEKETGLKVKVVAGTDSEWFTKLKA
SNGRNAPYDLLILQPDTIHRAQSAGLLAPIDQEKVPNVRDLYHSVQKRFTDDGKQYAAGFSMGQLGIVYRKDLVPQEPKR
WTDLWNSSYKGHVAISSPAYSAGLQFFVGLINAQGGLESNGKDVDKAFNSLADLKNNITAYPDNPGSIQTLLERGDAWVV
PFWDGRAFSLQDAGLDLGFAYPEEGAVAAVASWALMKGSPNQDNAYKLLNHLLSPEVQGDFADRTFYGMANKKVKYSDKL
KGKIKVGEDYYRNLKWVDYEVAAKDLGDWTNRWNQVLGGK

Sequences:

>Translated_360_residues
MKSKISALFILSLAAVLLLSGCGGNGSQESKQSDTVVIGVYGGDWEKHIKPSLESFEKETGLKVKVVAGTDSEWFTKLKA
SNGRNAPYDLLILQPDTIHRAQSAGLLAPIDQEKVPNVRDLYHSVQKRFTDDGKQYAAGFSMGQLGIVYRKDLVPQEPKR
WTDLWNSSYKGHVAISSPAYSAGLQFFVGLINAQGGLESNGKDVDKAFNSLADLKNNITAYPDNPGSIQTLLERGDAWVV
PFWDGRAFSLQDAGLDLGFAYPEEGAVAAVASWALMKGSPNQDNAYKLLNHLLSPEVQGDFADRTFYGMANKKVKYSDKL
KGKIKVGEDYYRNLKWVDYEVAAKDLGDWTNRWNQVLGGK
>Mature_360_residues
MKSKISALFILSLAAVLLLSGCGGNGSQESKQSDTVVIGVYGGDWEKHIKPSLESFEKETGLKVKVVAGTDSEWFTKLKA
SNGRNAPYDLLILQPDTIHRAQSAGLLAPIDQEKVPNVRDLYHSVQKRFTDDGKQYAAGFSMGQLGIVYRKDLVPQEPKR
WTDLWNSSYKGHVAISSPAYSAGLQFFVGLINAQGGLESNGKDVDKAFNSLADLKNNITAYPDNPGSIQTLLERGDAWVV
PFWDGRAFSLQDAGLDLGFAYPEEGAVAAVASWALMKGSPNQDNAYKLLNHLLSPEVQGDFADRTFYGMANKKVKYSDKL
KGKIKVGEDYYRNLKWVDYEVAAKDLGDWTNRWNQVLGGK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=276, Percent_Identity=27.536231884058, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787078, Length=292, Percent_Identity=28.0821917808219, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 39625; Mature: 39625

Theoretical pI: Translated: 7.68; Mature: 7.68

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSKISALFILSLAAVLLLSGCGGNGSQESKQSDTVVIGVYGGDWEKHIKPSLESFEKET
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCHHHCCHHHHHHHHHC
GLKVKVVAGTDSEWFTKLKASNGRNAPYDLLILQPDTIHRAQSAGLLAPIDQEKVPNVRD
CCEEEEEECCCHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHCCCCCCCCCHHHCCCHHH
LYHSVQKRFTDDGKQYAAGFSMGQLGIVYRKDLVPQEPKRWTDLWNSSYKGHVAISSPAY
HHHHHHHHHCCCCHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHCCCCCCEEEECCCCH
SAGLQFFVGLINAQGGLESNGKDVDKAFNSLADLKNNITAYPDNPGSIQTLLERGDAWVV
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCEECCCCCHHHHHHHHCCCEEEE
PFWDGRAFSLQDAGLDLGFAYPEEGAVAAVASWALMKGSPNQDNAYKLLNHLLSPEVQGD
EECCCCEEEEECCCCEEEECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCC
FADRTFYGMANKKVKYSDKLKGKIKVGEDYYRNLKWVDYEVAAKDLGDWTNRWNQVLGGK
CCCCEEECCCCCCEEECCCCCCEEEECHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKSKISALFILSLAAVLLLSGCGGNGSQESKQSDTVVIGVYGGDWEKHIKPSLESFEKET
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCHHHCCHHHHHHHHHC
GLKVKVVAGTDSEWFTKLKASNGRNAPYDLLILQPDTIHRAQSAGLLAPIDQEKVPNVRD
CCEEEEEECCCHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHCCCCCCCCCHHHCCCHHH
LYHSVQKRFTDDGKQYAAGFSMGQLGIVYRKDLVPQEPKRWTDLWNSSYKGHVAISSPAY
HHHHHHHHHCCCCHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHCCCCCCEEEECCCCH
SAGLQFFVGLINAQGGLESNGKDVDKAFNSLADLKNNITAYPDNPGSIQTLLERGDAWVV
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCEECCCCCHHHHHHHHCCCEEEE
PFWDGRAFSLQDAGLDLGFAYPEEGAVAAVASWALMKGSPNQDNAYKLLNHLLSPEVQGD
EECCCCEEEEECCCCEEEECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCC
FADRTFYGMANKKVKYSDKLKGKIKVGEDYYRNLKWVDYEVAAKDLGDWTNRWNQVLGGK
CCCCEEECCCCCCEEECCCCCCEEEECHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]