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The map label for this gene is hyuB [H]

Identifier: 52784218

GI number: 52784218

Start: 372080

End: 374074

Strand: Direct

Name: hyuB [H]

Synonym: BLi00396

Alternate gene names: 52784218

Gene position: 372080-374074 (Clockwise)

Preceding gene: 52784217

Following gene: 52784219

Centisome position: 8.81

GC content: 50.98

Gene sequence:

>1995_bases
ATGCGCACAGATCCTGCCCGGCTGGAAATGATGAGAAGCTACTTTAACGCGATAGCCGCGGGAATGGGGCACGTTATTGA
ACGGACGTCCTTTACCACATTTGTAAAAGAATCGGCTGATTTCGCTACTGCGCTTGCGACGCCGGAAGGGGAGTTTTTCG
TTTATCCGAAAACGGTCGGCGTCACGATTTTTTTAGGGCTTTCATTAAAACGGGTCATCGAAGAAAGCGGCCCATTTGAA
CCGGGAGATATTATCCTCACAAACGATCCTTATACAACGGACGGATTGGCGACCCACTTGCCTGATATTCATGTGGTGAA
GCCGATTTTCTTGGAGGGCGAGATAATCAGTTACGCGTGGTGCTTTGTCCATTGCAGCGATGTCGGCGGCCTTGTCCCGG
CAAGCATTTCGCCGACCGCGACAGACATCCATCAGGAAGGCCTGAGAATACCGCCGGCAAGGATTTACAAAAAGGGGAGA
GAAAATCGCGATATATTGAAGGTTTTGCGGGCAAACAGCAGGGTTCCCGACTTAAATGAAGGGGATATTCATGCGATGGT
GGCCGCGGTCAATACGGCGGAGAATCGATTGACAGAAATGGTCGGAAAGTTTGGAAAAGAAGCGGTCCAACAAGGAATGG
ATGATTTGCTCGAACAAGCCGCGATGAGAGCCGGACGGGTAATCAGCAGGATTCCGGAAGGTTCATACAGTTTTTCAGAT
TATCTTGATGACGATATGGTTTCTGACGTTCCGATCCGCTTGGCTGTTACGGCAAACGTCTCGGGAGGGGAGATCACATT
GGACTTTTCAGACTGTGATCCTCAGGTGAAGACCGCATTTAACCTTGTCACCAATGGAAGCAAACATTCATTTCTATATC
AGGGCCTGATCAACTATATCATAAGTGAAGACCCGTATATTCCGATTAACGGGGGGCTGACACGGCCGATCCAAGTTATC
GCTCCAAAGGGTACGCTGGTTCATCCGGAATATCCTGCGGCGGTCGGCATTCGTCATTCGATCACGATGAGACTTTATAA
TGCTGTACTGGGCGCATTGGCAAAGGCGCTTCCGAAAGCCGTGCCTGCAGCAGGCGCCGGTCAGTCAGCGATTGTCGTAC
TGTCGGTTCCCGATGAATCAACTGGCAGCAGGAAGATGTCGGTGGTCGAACCGATGGGAGGCGGAGGAGGCGGCCAGCAT
GACATGGACGGAGCAGATGGAATTGACCATGCCTCCGGATTTTTGAAAAATACGCCGATTGAAAGCCTTGAACAGCACAT
CGACATCCATGTTCGCCGCTATGAATTGATCAAAAATACAGCCGGAGCCGGTTTTCACAGGGGAGGCCATGCAATCGGGC
TGGAGTTTGAAGCGGCGGCACCAGAATCAATTGTGACCGCAAGAGGGATGGAGAGGATGCGTTTTCATCCGTGGGGGCTC
GCCGGGGGAACGGCAGGAGCGCTCGGGCGGGTGCGCCTTTATCCTGATCAGCCTCAGGAGAAAACGATGTCGAAGATCGA
TGTTCTAAAGCTCAATAAAGGGGATGTTATCAGCATTCATTCACCAGGCGGCGGAGGCTGGGGAAATCCCTTCCGCCGGG
AAGCTGAATCGATTTTAGGAGAAGTTGAAGCGGGCTTGCTGAGCCCGGAAGCGGCATATCATCAATACGGCGTTGCTGTA
AAAGAAAACGGCGCTGGCTCCTTCATTATTGATCTGGAAGAAACAGCTGAAATCAGAAAAAAGAAAGCAGGCGAACATCG
GAAACAATGGGATTTCGGAATCGCCAGAGAAGAATACGAACGGTGCTGGACAGAAGAAGCCAGCAGCACATTGGCCCGGC
TTCTGCGACAGCTTCCGGCTCATCAAAGGTCACATGTTAAACATTTAGTCCATCGTTATTTCTCAAAAGAACATAACGGT
CCGATCAGTAAAAGCGAAGTTGCGGCCGCTGTGGAAAAACTGGCTGGAAAAAATAACACGGGAATGGTGAGATGA

Upstream 100 bases:

>100_bases
TTGTTGAGCAGGACGATACAACGACGCTGATTTTGCCAAATTGGAGCGGGAAAACCGATCATGCCGGAAATTTGATCATT
GAACGGAAGGAGAGCTGACC

Downstream 100 bases:

>100_bases
CAATGAAAAGTAAAATTTCTGCTTTATTTATTCTGTCTCTTGCAGCGGTGCTCCTCCTTTCGGGATGCGGCGGAAACGGA
AGTCAGGAAAGCAAACAATC

Product: hypothetical protein

Products: ADP; phosphate; N-carbamoylsarcosine

Alternate protein names: ORF3 [H]

Number of amino acids: Translated: 664; Mature: 664

Protein sequence:

>664_residues
MRTDPARLEMMRSYFNAIAAGMGHVIERTSFTTFVKESADFATALATPEGEFFVYPKTVGVTIFLGLSLKRVIEESGPFE
PGDIILTNDPYTTDGLATHLPDIHVVKPIFLEGEIISYAWCFVHCSDVGGLVPASISPTATDIHQEGLRIPPARIYKKGR
ENRDILKVLRANSRVPDLNEGDIHAMVAAVNTAENRLTEMVGKFGKEAVQQGMDDLLEQAAMRAGRVISRIPEGSYSFSD
YLDDDMVSDVPIRLAVTANVSGGEITLDFSDCDPQVKTAFNLVTNGSKHSFLYQGLINYIISEDPYIPINGGLTRPIQVI
APKGTLVHPEYPAAVGIRHSITMRLYNAVLGALAKALPKAVPAAGAGQSAIVVLSVPDESTGSRKMSVVEPMGGGGGGQH
DMDGADGIDHASGFLKNTPIESLEQHIDIHVRRYELIKNTAGAGFHRGGHAIGLEFEAAAPESIVTARGMERMRFHPWGL
AGGTAGALGRVRLYPDQPQEKTMSKIDVLKLNKGDVISIHSPGGGGWGNPFRREAESILGEVEAGLLSPEAAYHQYGVAV
KENGAGSFIIDLEETAEIRKKKAGEHRKQWDFGIAREEYERCWTEEASSTLARLLRQLPAHQRSHVKHLVHRYFSKEHNG
PISKSEVAAAVEKLAGKNNTGMVR

Sequences:

>Translated_664_residues
MRTDPARLEMMRSYFNAIAAGMGHVIERTSFTTFVKESADFATALATPEGEFFVYPKTVGVTIFLGLSLKRVIEESGPFE
PGDIILTNDPYTTDGLATHLPDIHVVKPIFLEGEIISYAWCFVHCSDVGGLVPASISPTATDIHQEGLRIPPARIYKKGR
ENRDILKVLRANSRVPDLNEGDIHAMVAAVNTAENRLTEMVGKFGKEAVQQGMDDLLEQAAMRAGRVISRIPEGSYSFSD
YLDDDMVSDVPIRLAVTANVSGGEITLDFSDCDPQVKTAFNLVTNGSKHSFLYQGLINYIISEDPYIPINGGLTRPIQVI
APKGTLVHPEYPAAVGIRHSITMRLYNAVLGALAKALPKAVPAAGAGQSAIVVLSVPDESTGSRKMSVVEPMGGGGGGQH
DMDGADGIDHASGFLKNTPIESLEQHIDIHVRRYELIKNTAGAGFHRGGHAIGLEFEAAAPESIVTARGMERMRFHPWGL
AGGTAGALGRVRLYPDQPQEKTMSKIDVLKLNKGDVISIHSPGGGGWGNPFRREAESILGEVEAGLLSPEAAYHQYGVAV
KENGAGSFIIDLEETAEIRKKKAGEHRKQWDFGIAREEYERCWTEEASSTLARLLRQLPAHQRSHVKHLVHRYFSKEHNG
PISKSEVAAAVEKLAGKNNTGMVR
>Mature_664_residues
MRTDPARLEMMRSYFNAIAAGMGHVIERTSFTTFVKESADFATALATPEGEFFVYPKTVGVTIFLGLSLKRVIEESGPFE
PGDIILTNDPYTTDGLATHLPDIHVVKPIFLEGEIISYAWCFVHCSDVGGLVPASISPTATDIHQEGLRIPPARIYKKGR
ENRDILKVLRANSRVPDLNEGDIHAMVAAVNTAENRLTEMVGKFGKEAVQQGMDDLLEQAAMRAGRVISRIPEGSYSFSD
YLDDDMVSDVPIRLAVTANVSGGEITLDFSDCDPQVKTAFNLVTNGSKHSFLYQGLINYIISEDPYIPINGGLTRPIQVI
APKGTLVHPEYPAAVGIRHSITMRLYNAVLGALAKALPKAVPAAGAGQSAIVVLSVPDESTGSRKMSVVEPMGGGGGGQH
DMDGADGIDHASGFLKNTPIESLEQHIDIHVRRYELIKNTAGAGFHRGGHAIGLEFEAAAPESIVTARGMERMRFHPWGL
AGGTAGALGRVRLYPDQPQEKTMSKIDVLKLNKGDVISIHSPGGGGWGNPFRREAESILGEVEAGLLSPEAAYHQYGVAV
KENGAGSFIIDLEETAEIRKKKAGEHRKQWDFGIAREEYERCWTEEASSTLARLLRQLPAHQRSHVKHLVHRYFSKEHNG
PISKSEVAAAVEKLAGKNNTGMVR

Specific function: HyuA and hyuB convert D- and L-substituted hydantoins to corresponding N-carbamyl-amino acids [H]

COG id: COG0146

COG function: function code EQ; N-methylhydantoinase B/acetone carboxylase, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the oxoprolinase family [H]

Homologues:

Organism=Homo sapiens, GI48314820, Length=553, Percent_Identity=28.5714285714286, Blast_Score=182, Evalue=7e-46,
Organism=Caenorhabditis elegans, GI133901900, Length=552, Percent_Identity=27.8985507246377, Blast_Score=168, Evalue=9e-42,
Organism=Caenorhabditis elegans, GI133901902, Length=527, Percent_Identity=27.3244781783681, Blast_Score=142, Evalue=6e-34,
Organism=Saccharomyces cerevisiae, GI6322634, Length=565, Percent_Identity=26.0176991150442, Blast_Score=140, Evalue=8e-34,
Organism=Drosophila melanogaster, GI45550492, Length=564, Percent_Identity=27.3049645390071, Blast_Score=146, Evalue=5e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003692 [H]

Pfam domain/function: PF02538 Hydantoinase_B [H]

EC number: 3.5.2.14

Molecular weight: Translated: 72097; Mature: 72097

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTDPARLEMMRSYFNAIAAGMGHVIERTSFTTFVKESADFATALATPEGEFFVYPKTVG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEECCCCCEEEEECCCC
VTIFLGLSLKRVIEESGPFEPGDIILTNDPYTTDGLATHLPDIHVVKPIFLEGEIISYAW
EEEEEHHHHHHHHHHCCCCCCCCEEECCCCCCCCCHHHHCCCCEEECEEEECCHHHEEEE
CFVHCSDVGGLVPASISPTATDIHQEGLRIPPARIYKKGRENRDILKVLRANSRVPDLNE
EEEEECCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHHCCCCCCCCC
GDIHAMVAAVNTAENRLTEMVGKFGKEAVQQGMDDLLEQAAMRAGRVISRIPEGSYSFSD
CCCEEHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
YLDDDMVSDVPIRLAVTANVSGGEITLDFSDCDPQVKTAFNLVTNGSKHSFLYQGLINYI
HCCCCHHCCCCEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHH
ISEDPYIPINGGLTRPIQVIAPKGTLVHPEYPAAVGIRHSITMRLYNAVLGALAKALPKA
HCCCCEEECCCCCCCCEEEEECCCCEECCCCCCHHCHHHHHHHHHHHHHHHHHHHHHHHH
VPAAGAGQSAIVVLSVPDESTGSRKMSVVEPMGGGGGGQHDMDGADGIDHASGFLKNTPI
CCCCCCCCCEEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHCCCCH
ESLEQHIDIHVRRYELIKNTAGAGFHRGGHAIGLEFEAAAPESIVTARGMERMRFHPWGL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHCHHHHCCCCCCC
AGGTAGALGRVRLYPDQPQEKTMSKIDVLKLNKGDVISIHSPGGGGWGNPFRREAESILG
CCCCCCCCCEEEECCCCCHHHHHHHHEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHH
EVEAGLLSPEAAYHQYGVAVKENGAGSFIIDLEETAEIRKKKAGEHRKQWDFGIAREEYE
HHHHCCCCCHHHHHHHCCEEEECCCCEEEEECHHHHHHHHHHCCCHHHHCCCCCCHHHHH
RCWTEEASSTLARLLRQLPAHQRSHVKHLVHRYFSKEHNGPISKSEVAAAVEKLAGKNNT
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCC
GMVR
CCCC
>Mature Secondary Structure
MRTDPARLEMMRSYFNAIAAGMGHVIERTSFTTFVKESADFATALATPEGEFFVYPKTVG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEECCCCCEEEEECCCC
VTIFLGLSLKRVIEESGPFEPGDIILTNDPYTTDGLATHLPDIHVVKPIFLEGEIISYAW
EEEEEHHHHHHHHHHCCCCCCCCEEECCCCCCCCCHHHHCCCCEEECEEEECCHHHEEEE
CFVHCSDVGGLVPASISPTATDIHQEGLRIPPARIYKKGRENRDILKVLRANSRVPDLNE
EEEEECCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHHCCCCCCCCC
GDIHAMVAAVNTAENRLTEMVGKFGKEAVQQGMDDLLEQAAMRAGRVISRIPEGSYSFSD
CCCEEHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
YLDDDMVSDVPIRLAVTANVSGGEITLDFSDCDPQVKTAFNLVTNGSKHSFLYQGLINYI
HCCCCHHCCCCEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHH
ISEDPYIPINGGLTRPIQVIAPKGTLVHPEYPAAVGIRHSITMRLYNAVLGALAKALPKA
HCCCCEEECCCCCCCCEEEEECCCCEECCCCCCHHCHHHHHHHHHHHHHHHHHHHHHHHH
VPAAGAGQSAIVVLSVPDESTGSRKMSVVEPMGGGGGGQHDMDGADGIDHASGFLKNTPI
CCCCCCCCCEEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHCCCCH
ESLEQHIDIHVRRYELIKNTAGAGFHRGGHAIGLEFEAAAPESIVTARGMERMRFHPWGL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHCHHHHCCCCCCC
AGGTAGALGRVRLYPDQPQEKTMSKIDVLKLNKGDVISIHSPGGGGWGNPFRREAESILG
CCCCCCCCCEEEECCCCCHHHHHHHHEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHH
EVEAGLLSPEAAYHQYGVAVKENGAGSFIIDLEETAEIRKKKAGEHRKQWDFGIAREEYE
HHHHCCCCCHHHHHHHCCEEEECCCCEEEEECHHHHHHHHHHCCCHHHHCCCCCCHHHHH
RCWTEEASSTLARLLRQLPAHQRSHVKHLVHRYFSKEHNGPISKSEVAAAVEKLAGKNNT
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCC
GMVR
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; N-methylimidazolidine-2,4-dione; H2O

Specific reaction: ATP + N-methylimidazolidine-2,4-dione + 2 H2O = ADP + phosphate + N-carbamoylsarcosine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1732229 [H]