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The map label for this gene is ywkA [H]

Identifier: 229312736

GI number: 229312736

Start: 3776975

End: 3778678

Strand: Reverse

Name: ywkA [H]

Synonym: BLi03953

Alternate gene names: 229312736

Gene position: 3778678-3776975 (Counterclockwise)

Preceding gene: 52787632

Following gene: 52787630

Centisome position: 89.49

GC content: 47.65

Gene sequence:

>1704_bases
GTGAACAACATGAGAAGAACGAAGGAAGGACATTTGGAGACTTCTTTGCGGGGTAAAGACGTTCTTTCCATTCCAACGCT
AAATAAAGGTGTTGCATTTTCTCTTGAAGAAAGAAAGAACCTCGGTCTAGAAGGGCTTCTGCCGCCGACTGTTCTCAGCC
TTGATCAGCAGGCAGAACGGGCTTACGAACAATTTTCCCGTCAACCTGACAGACTTCGCCAAAACGTATATTTGAACGAT
CTGCAAAACAGAAACGAAGTGCTTTTCTATAAATTATTGCAAAACCACCTGCGTGAAATGCTTCCGGTTGTCTATACGCC
TACTGTCGGCGAAGCGATTCAGGAATACAGCCACGAATACCGCAGACCGCACGGCGTTTACTTGTCAATCGATGATATGG
ACGGAATCGAAACGGCTTTTAAAAACCTGCAGGCATCTGCTGATGACATCGATTTGATCGTCGCTACAGATTCCGAAAGC
ATTTTGGGGATCGGCGACTGGGGCGTCGGAGGCATCAATATTGCGATTGGTAAGCTTGCAGTATATACGGCGGCAGCGGG
AATCGATCCGAGCCGCGTTATCCCGGTCGTACTGGATGTCGGAACGAATAATGAAAAACTGCTAAACGATCCGCTCTATA
TCGGAAACCGTCATCCGCGTGTAGAAGGCGAGCGCTATGAACAATTTATCGACGCTTATGTCGAAACAGCATTGAAGCTT
TTCCCTAACGCGCTTCTACACTGGGAAGATTTCGGAAATAAAAATGCCCGCAATATCATGAGTAAATATAACCATAAAAT
TGCAACATTTAATGATGATATTCAAGGAACAGGGGCTATCACGCTGGCTGCGATTTTAGCAGCTGTCAAAAAGACCGGCA
CGTCTTTGAAAGACCACCGGGTCGTCATCTTCGGACCTGGTTCTGCTGGTATAGGAATCGCTGACCAAATCCGCGACACC
ATGGTGCTTGAAGGGCTTTCAGAAGAAGAAGCCAACCGTGCATTCTGGACAGTCGATTACAGAGGATTATTAACGACAGG
CATGGATCAAATCATGGATTTCCAAGAACCGTATTTGCGCGATACGGAAGAAGTGAAAGATTGGAAACGCAATGAAAAGG
GAGAGATTTCCTTTGAGGAAGTCATCCGTCAAGTGAAGCCTACGATCCTGATTGGTACATCAGGTGTTGCCGGCGCCTTT
AGTGAAGAAATCATCAAAGAGATGGCGGCTCATACTGAGCGTCCTGTGATTCTGCCGATGTCAAATCCGACACATCTGGC
CGAAGCTGTTCCTGAAGACTTGTTCAAATGGACGGACGGAAAAGCGCTTGTGGCGACGGGAAGCCCGTTTGAAGCTGTTG
AGTACAATGGCGTGAAACACGAAATCGGACAATCCAATAACGCTTTTGTATTCCCTGGATTAGGCCTGGGTACAATCGTT
GCGAAAGCGAAAGTCATCACAAACGGCATGTTTGCTGCATCTGCCAACGCGGTCGCGAACATGGTGGATCATGAAAAGCC
GGGTGCAGGTCTTCTGCCGAAAATCGACGACCTGCAAGACGTATCCGTTCAAGTGGCGATCGACGTTGTAAAAGCTGCCA
TTAAAGACGGCGTGGCCCAAGTTCAGCCTGAAGATGTCGAAAAGGCTGTCCGCGAAGCCATGTGGCGTCCGGAGTATAAA
ACAATTGTTGCAAAACAAGACTAA

Upstream 100 bases:

>100_bases
ACATATTTGACAAATCTACATGTGTAAAAAGGCAAATATTCTATATTGTTTACCGTATTTATTTCACTTCATTAACATTT
TAAGAAAGAAGGTAATTAGT

Downstream 100 bases:

>100_bases
TATTAGGTTCTGAGGAAACGATAAAAGAAACGGCAGCCCGGGTGCGGATGTTTTGTCACCGGGCTGCACCAATCAAATTT
GAAAAAAATGGGATCGGCGG

Product: malate dehydrogenase

Products: NA

Alternate protein names: NAD-ME 2 [H]

Number of amino acids: Translated: 567; Mature: 567

Protein sequence:

>567_residues
MNNMRRTKEGHLETSLRGKDVLSIPTLNKGVAFSLEERKNLGLEGLLPPTVLSLDQQAERAYEQFSRQPDRLRQNVYLND
LQNRNEVLFYKLLQNHLREMLPVVYTPTVGEAIQEYSHEYRRPHGVYLSIDDMDGIETAFKNLQASADDIDLIVATDSES
ILGIGDWGVGGINIAIGKLAVYTAAAGIDPSRVIPVVLDVGTNNEKLLNDPLYIGNRHPRVEGERYEQFIDAYVETALKL
FPNALLHWEDFGNKNARNIMSKYNHKIATFNDDIQGTGAITLAAILAAVKKTGTSLKDHRVVIFGPGSAGIGIADQIRDT
MVLEGLSEEEANRAFWTVDYRGLLTTGMDQIMDFQEPYLRDTEEVKDWKRNEKGEISFEEVIRQVKPTILIGTSGVAGAF
SEEIIKEMAAHTERPVILPMSNPTHLAEAVPEDLFKWTDGKALVATGSPFEAVEYNGVKHEIGQSNNAFVFPGLGLGTIV
AKAKVITNGMFAASANAVANMVDHEKPGAGLLPKIDDLQDVSVQVAIDVVKAAIKDGVAQVQPEDVEKAVREAMWRPEYK
TIVAKQD

Sequences:

>Translated_567_residues
MNNMRRTKEGHLETSLRGKDVLSIPTLNKGVAFSLEERKNLGLEGLLPPTVLSLDQQAERAYEQFSRQPDRLRQNVYLND
LQNRNEVLFYKLLQNHLREMLPVVYTPTVGEAIQEYSHEYRRPHGVYLSIDDMDGIETAFKNLQASADDIDLIVATDSES
ILGIGDWGVGGINIAIGKLAVYTAAAGIDPSRVIPVVLDVGTNNEKLLNDPLYIGNRHPRVEGERYEQFIDAYVETALKL
FPNALLHWEDFGNKNARNIMSKYNHKIATFNDDIQGTGAITLAAILAAVKKTGTSLKDHRVVIFGPGSAGIGIADQIRDT
MVLEGLSEEEANRAFWTVDYRGLLTTGMDQIMDFQEPYLRDTEEVKDWKRNEKGEISFEEVIRQVKPTILIGTSGVAGAF
SEEIIKEMAAHTERPVILPMSNPTHLAEAVPEDLFKWTDGKALVATGSPFEAVEYNGVKHEIGQSNNAFVFPGLGLGTIV
AKAKVITNGMFAASANAVANMVDHEKPGAGLLPKIDDLQDVSVQVAIDVVKAAIKDGVAQVQPEDVEKAVREAMWRPEYK
TIVAKQD
>Mature_567_residues
MNNMRRTKEGHLETSLRGKDVLSIPTLNKGVAFSLEERKNLGLEGLLPPTVLSLDQQAERAYEQFSRQPDRLRQNVYLND
LQNRNEVLFYKLLQNHLREMLPVVYTPTVGEAIQEYSHEYRRPHGVYLSIDDMDGIETAFKNLQASADDIDLIVATDSES
ILGIGDWGVGGINIAIGKLAVYTAAAGIDPSRVIPVVLDVGTNNEKLLNDPLYIGNRHPRVEGERYEQFIDAYVETALKL
FPNALLHWEDFGNKNARNIMSKYNHKIATFNDDIQGTGAITLAAILAAVKKTGTSLKDHRVVIFGPGSAGIGIADQIRDT
MVLEGLSEEEANRAFWTVDYRGLLTTGMDQIMDFQEPYLRDTEEVKDWKRNEKGEISFEEVIRQVKPTILIGTSGVAGAF
SEEIIKEMAAHTERPVILPMSNPTHLAEAVPEDLFKWTDGKALVATGSPFEAVEYNGVKHEIGQSNNAFVFPGLGLGTIV
AKAKVITNGMFAASANAVANMVDHEKPGAGLLPKIDDLQDVSVQVAIDVVKAAIKDGVAQVQPEDVEKAVREAMWRPEYK
TIVAKQD

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family [H]

Homologues:

Organism=Homo sapiens, GI62420882, Length=551, Percent_Identity=41.923774954628, Blast_Score=440, Evalue=1e-123,
Organism=Homo sapiens, GI62420880, Length=551, Percent_Identity=41.923774954628, Blast_Score=440, Evalue=1e-123,
Organism=Homo sapiens, GI239049447, Length=551, Percent_Identity=41.923774954628, Blast_Score=440, Evalue=1e-123,
Organism=Homo sapiens, GI4505143, Length=556, Percent_Identity=41.0071942446043, Blast_Score=428, Evalue=1e-120,
Organism=Homo sapiens, GI4505145, Length=560, Percent_Identity=39.1071428571429, Blast_Score=409, Evalue=1e-114,
Organism=Homo sapiens, GI270265879, Length=467, Percent_Identity=41.1134903640257, Blast_Score=364, Evalue=1e-100,
Organism=Escherichia coli, GI87081919, Length=558, Percent_Identity=43.9068100358423, Blast_Score=478, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI17537199, Length=556, Percent_Identity=41.3669064748201, Blast_Score=431, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6322823, Length=584, Percent_Identity=42.2945205479452, Blast_Score=492, Evalue=1e-140,
Organism=Drosophila melanogaster, GI281362672, Length=539, Percent_Identity=40.0742115027829, Blast_Score=410, Evalue=1e-115,
Organism=Drosophila melanogaster, GI281362674, Length=539, Percent_Identity=40.0742115027829, Blast_Score=410, Evalue=1e-114,
Organism=Drosophila melanogaster, GI21356279, Length=539, Percent_Identity=40.0742115027829, Blast_Score=410, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24646388, Length=549, Percent_Identity=39.5264116575592, Blast_Score=400, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24646386, Length=549, Percent_Identity=39.5264116575592, Blast_Score=399, Evalue=1e-111,
Organism=Drosophila melanogaster, GI281363505, Length=537, Percent_Identity=35.3817504655494, Blast_Score=380, Evalue=1e-105,
Organism=Drosophila melanogaster, GI78707236, Length=537, Percent_Identity=35.3817504655494, Blast_Score=380, Evalue=1e-105,
Organism=Drosophila melanogaster, GI281363503, Length=539, Percent_Identity=35.0649350649351, Blast_Score=377, Evalue=1e-104,
Organism=Drosophila melanogaster, GI78707232, Length=512, Percent_Identity=36.328125, Blast_Score=372, Evalue=1e-103,
Organism=Drosophila melanogaster, GI78707242, Length=546, Percent_Identity=36.2637362637363, Blast_Score=367, Evalue=1e-101,
Organism=Drosophila melanogaster, GI78707238, Length=546, Percent_Identity=36.2637362637363, Blast_Score=367, Evalue=1e-101,
Organism=Drosophila melanogaster, GI78707240, Length=516, Percent_Identity=36.8217054263566, Blast_Score=354, Evalue=9e-98,
Organism=Drosophila melanogaster, GI19922384, Length=563, Percent_Identity=27.5310834813499, Blast_Score=247, Evalue=1e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M [H]

EC number: =1.1.1.38 [H]

Molecular weight: Translated: 62649; Mature: 62649

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNMRRTKEGHLETSLRGKDVLSIPTLNKGVAFSLEERKNLGLEGLLPPTVLSLDQQAER
CCCCCCCCCCCCHHCCCCCCEEECCCCCCCCEEEEHHHHCCCCCCCCCHHHHHHHHHHHH
AYEQFSRQPDRLRQNVYLNDLQNRNEVLFYKLLQNHLREMLPVVYTPTVGEAIQEYSHEY
HHHHHCCCHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHH
RRPHGVYLSIDDMDGIETAFKNLQASADDIDLIVATDSESILGIGDWGVGGINIAIGKLA
CCCCCEEEEECCCCHHHHHHHHHCCCCCCEEEEEEECCCCEEEECCCCCCCEEEEHHHHH
VYTAAAGIDPSRVIPVVLDVGTNNEKLLNDPLYIGNRHPRVEGERYEQFIDAYVETALKL
HHHHHCCCCCCCEEEEEEEECCCCCCEECCCEEECCCCCCCCCHHHHHHHHHHHHHHHHH
FPNALLHWEDFGNKNARNIMSKYNHKIATFNDDIQGTGAITLAAILAAVKKTGTSLKDHR
HHHHHEEHHHCCCCCHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCE
VVIFGPGSAGIGIADQIRDTMVLEGLSEEEANRAFWTVDYRGLLTTGMDQIMDFQEPYLR
EEEEECCCCCCCHHHHHHHHHHHCCCCHHCCCCEEEEEECCHHHHHHHHHHHHCCCHHCC
DTEEVKDWKRNEKGEISFEEVIRQVKPTILIGTSGVAGAFSEEIIKEMAAHTERPVILPM
CHHHHHHHHCCCCCCCCHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEC
SNPTHLAEAVPEDLFKWTDGKALVATGSPFEAVEYNGVKHEIGQSNNAFVFPGLGLGTIV
CCCHHHHHHHHHHHHHCCCCCEEEECCCCCCHHCCCCCHHHCCCCCCEEEECCCCHHHHH
AKAKVITNGMFAASANAVANMVDHEKPGAGLLPKIDDLQDVSVQVAIDVVKAAIKDGVAQ
HHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHC
VQPEDVEKAVREAMWRPEYKTIVAKQD
CCHHHHHHHHHHHHCCCCHHEEEECCC
>Mature Secondary Structure
MNNMRRTKEGHLETSLRGKDVLSIPTLNKGVAFSLEERKNLGLEGLLPPTVLSLDQQAER
CCCCCCCCCCCCHHCCCCCCEEECCCCCCCCEEEEHHHHCCCCCCCCCHHHHHHHHHHHH
AYEQFSRQPDRLRQNVYLNDLQNRNEVLFYKLLQNHLREMLPVVYTPTVGEAIQEYSHEY
HHHHHCCCHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHH
RRPHGVYLSIDDMDGIETAFKNLQASADDIDLIVATDSESILGIGDWGVGGINIAIGKLA
CCCCCEEEEECCCCHHHHHHHHHCCCCCCEEEEEEECCCCEEEECCCCCCCEEEEHHHHH
VYTAAAGIDPSRVIPVVLDVGTNNEKLLNDPLYIGNRHPRVEGERYEQFIDAYVETALKL
HHHHHCCCCCCCEEEEEEEECCCCCCEECCCEEECCCCCCCCCHHHHHHHHHHHHHHHHH
FPNALLHWEDFGNKNARNIMSKYNHKIATFNDDIQGTGAITLAAILAAVKKTGTSLKDHR
HHHHHEEHHHCCCCCHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCE
VVIFGPGSAGIGIADQIRDTMVLEGLSEEEANRAFWTVDYRGLLTTGMDQIMDFQEPYLR
EEEEECCCCCCCHHHHHHHHHHHCCCCHHCCCCEEEEEECCHHHHHHHHHHHHCCCHHCC
DTEEVKDWKRNEKGEISFEEVIRQVKPTILIGTSGVAGAFSEEIIKEMAAHTERPVILPM
CHHHHHHHHCCCCCCCCHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEC
SNPTHLAEAVPEDLFKWTDGKALVATGSPFEAVEYNGVKHEIGQSNNAFVFPGLGLGTIV
CCCHHHHHHHHHHHHHCCCCCEEEECCCCCCHHCCCCCHHHCCCCCCEEEECCCCHHHHH
AKAKVITNGMFAASANAVANMVDHEKPGAGLLPKIDDLQDVSVQVAIDVVKAAIKDGVAQ
HHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHC
VQPEDVEKAVREAMWRPEYKTIVAKQD
CCHHHHHHHHHHHHCCCCHHEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 12949160 [H]