| Definition | Bacillus cereus E33L, complete genome. |
|---|---|
| Accession | NC_006274 |
| Length | 5,300,915 |
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The map label for this gene is cwlA [H]
Identifier: 52144447
GI number: 52144447
Start: 885484
End: 887223
Strand: Reverse
Name: cwlA [H]
Synonym: BCZK0777
Alternate gene names: 52144447
Gene position: 887223-885484 (Counterclockwise)
Preceding gene: 52144443
Following gene: 52144451
Centisome position: 16.74
GC content: 35.69
Gene sequence:
>1740_bases ATGAAAAAAAAGTTTTACAACATTTTAGCATTATCTTTGGCATTACAAACATTTGTAACGCCTACCCATTCATTCGCAGA AAGTATAGAAAAAACTGCGGAATTGTCTATAGATGAGCAAGCTTTAAAAAGCATTGAAGAACATGCAAAACAACATAGAC ATGACGAAGAAAACGACCTTGAAATCTCGGGTGAATATCTTCTCCACTTATCAACAAAACTTCCACTATACGATTCAAAC GACTTAAAAACCAAAACAAATATTGAAATTGCTGAACCAGTGGTAAAGGCAACGAAGAAAAAAGGAAACGCCTATTATAT ACAAACTATATCTGGTGCTGGCTGGGTTAATAAGGAAAATAATAATTTAGAAGTACAAGAGATTTATAAACTCTCGAATC AAAAATTAATTGTAACAGAAGAAACTTCAGTATATTCATTCCCTTTCCAATCATTTAAAGAAGAAACGAAATTACAGTCA CAAACAGTAGCTCCTACTGAACAAGCTGGGAATTGGTTTAAAATTCAAATGAACGGAACTGAGAAATGGATTTATGCTCC TTCAGCTACATTCGAAGGTACGAAAGCAACACTATTCGAAAAAAAAGTTTCTATGGAAAATAGAAGCGCCGCATTTTTCA AAACACCGTCTCATTTACCTACAAATAACGTTTATGGTGTTACATTTAAAGAAATGATTGTTCCAAAAGGGAATGAAAAT ATTCGTCCCGGCTACGCAATGGAACCGAAATACATTACAATTCATGAAACTGCTAATACAAGTAAAGGTGCGAACGCCTT AAATCATGCGAAGTACTTAGATAATCAAGCCCGCGGAAATACTGATCGTTCAGCATCTTGGCATTTTACAGTAGATGACA AAGAAATTTATCAACATTTACCATTAAATGAAGTTGGTTGGCATGCCGGAAATAAAACTGGAAACTATGAATCTATCGGA ATTGAAATTGCTGTAAATAGTGATGGAAATTATACGAAAGCAGTAGAAAATGCGCGCAAGTTAGCAGCTTATTTAATGAA CGAATTAAACATATCTTTAGATCACGTTCAAAAGCATCAATTTTGGAGCAAAAAAAACTGTCCGGCATTTATGATTCAAC GTGGACAATGGGATGCTTTCTTAAAGGGCACGAACGCTTACTATAATGAACATCATAAAGAGGTTATGCCACCGCCAGAA GTCCCTCATGAAAAAGACGACATTACAGGTGGTTGGTATGAACAAGACATTCGTCAATTAGCTGCTAAAAAAATCATGTT CGGTGACGGCAATGGTTCTTACTGGCCAAACCGTCTTGTCACACGAGCTGAGTTTGCAAGTTTAATCTCACGTTCTTTAA AATTACCTGATGGAAACGCTAAATTTATAGACTTAAACGAAGCACATCCATCTTTAGTAGATGGAATTAACCGCGCCGCT AGCGCAGGTATCATTAATGGTCGTGGGAATAATAAATTTGATCCTAATGCTACAATAACACGTGATGAAGCTGTTATTAT GATTGACCGTGCACTAAAATATAACTGGATTTATCGAAAAGAAGTGAAATTACCATTCACTGACCAACACTTAGCATACG ATAAAAAAGCATTACAAAACGTTTATGCATACGGAATTGTAAAAGGTAACGAACGAAATGAATTCGTACCAAAAGGCACA GCAACACGCGCTGAAGCAGCCGCATTTTTAAATCGAATGCTTAAAGTTATTGAAGCTTAA
Upstream 100 bases:
>100_bases TTGTTACAATCTAGAACGTATTAGTACTTGCTACTAATTTAAATACATATAAAGGAGGACTCTTAACACGCACGAAAGAC TATCACGTGTTAAAACAACT
Downstream 100 bases:
>100_bases ACAACAAAACTAAAAGGCATAAGAGATAACATTTCTCTTATGCCTTTTATAAAACTATTAACTATTTTTAATCATAAATC TAAGACGTATTCCTAATAAA
Product: N-acetylmuramoyl-L-alanine amidase
Products: NA
Alternate protein names: Autolysin; Cell wall hydrolase [H]
Number of amino acids: Translated: 579; Mature: 579
Protein sequence:
>579_residues MKKKFYNILALSLALQTFVTPTHSFAESIEKTAELSIDEQALKSIEEHAKQHRHDEENDLEISGEYLLHLSTKLPLYDSN DLKTKTNIEIAEPVVKATKKKGNAYYIQTISGAGWVNKENNNLEVQEIYKLSNQKLIVTEETSVYSFPFQSFKEETKLQS QTVAPTEQAGNWFKIQMNGTEKWIYAPSATFEGTKATLFEKKVSMENRSAAFFKTPSHLPTNNVYGVTFKEMIVPKGNEN IRPGYAMEPKYITIHETANTSKGANALNHAKYLDNQARGNTDRSASWHFTVDDKEIYQHLPLNEVGWHAGNKTGNYESIG IEIAVNSDGNYTKAVENARKLAAYLMNELNISLDHVQKHQFWSKKNCPAFMIQRGQWDAFLKGTNAYYNEHHKEVMPPPE VPHEKDDITGGWYEQDIRQLAAKKIMFGDGNGSYWPNRLVTRAEFASLISRSLKLPDGNAKFIDLNEAHPSLVDGINRAA SAGIINGRGNNKFDPNATITRDEAVIMIDRALKYNWIYRKEVKLPFTDQHLAYDKKALQNVYAYGIVKGNERNEFVPKGT ATRAEAAAFLNRMLKVIEA
Sequences:
>Translated_579_residues MKKKFYNILALSLALQTFVTPTHSFAESIEKTAELSIDEQALKSIEEHAKQHRHDEENDLEISGEYLLHLSTKLPLYDSN DLKTKTNIEIAEPVVKATKKKGNAYYIQTISGAGWVNKENNNLEVQEIYKLSNQKLIVTEETSVYSFPFQSFKEETKLQS QTVAPTEQAGNWFKIQMNGTEKWIYAPSATFEGTKATLFEKKVSMENRSAAFFKTPSHLPTNNVYGVTFKEMIVPKGNEN IRPGYAMEPKYITIHETANTSKGANALNHAKYLDNQARGNTDRSASWHFTVDDKEIYQHLPLNEVGWHAGNKTGNYESIG IEIAVNSDGNYTKAVENARKLAAYLMNELNISLDHVQKHQFWSKKNCPAFMIQRGQWDAFLKGTNAYYNEHHKEVMPPPE VPHEKDDITGGWYEQDIRQLAAKKIMFGDGNGSYWPNRLVTRAEFASLISRSLKLPDGNAKFIDLNEAHPSLVDGINRAA SAGIINGRGNNKFDPNATITRDEAVIMIDRALKYNWIYRKEVKLPFTDQHLAYDKKALQNVYAYGIVKGNERNEFVPKGT ATRAEAAAFLNRMLKVIEA >Mature_579_residues MKKKFYNILALSLALQTFVTPTHSFAESIEKTAELSIDEQALKSIEEHAKQHRHDEENDLEISGEYLLHLSTKLPLYDSN DLKTKTNIEIAEPVVKATKKKGNAYYIQTISGAGWVNKENNNLEVQEIYKLSNQKLIVTEETSVYSFPFQSFKEETKLQS QTVAPTEQAGNWFKIQMNGTEKWIYAPSATFEGTKATLFEKKVSMENRSAAFFKTPSHLPTNNVYGVTFKEMIVPKGNEN IRPGYAMEPKYITIHETANTSKGANALNHAKYLDNQARGNTDRSASWHFTVDDKEIYQHLPLNEVGWHAGNKTGNYESIG IEIAVNSDGNYTKAVENARKLAAYLMNELNISLDHVQKHQFWSKKNCPAFMIQRGQWDAFLKGTNAYYNEHHKEVMPPPE VPHEKDDITGGWYEQDIRQLAAKKIMFGDGNGSYWPNRLVTRAEFASLISRSLKLPDGNAKFIDLNEAHPSLVDGINRAA SAGIINGRGNNKFDPNATITRDEAVIMIDRALKYNWIYRKEVKLPFTDQHLAYDKKALQNVYAYGIVKGNERNEFVPKGT ATRAEAAAFLNRMLKVIEA
Specific function: Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation. Could play a role in mother cell lysis with CwlC [H]
COG id: COG5632
COG function: function code M; N-acetylmuramoyl-L-alanine amidase
Gene ontology:
Cell location: Secreted (Potential) [H]
Metaboloic importance: NA
Operon status: Not Known
Operon components: None
Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]
Homologues:
None
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002502 - InterPro: IPR002477 [H]
Pfam domain/function: PF01510 Amidase_2; PF01471 PG_binding_1 [H]
EC number: =3.5.1.28 [H]
Molecular weight: Translated: 65617; Mature: 65617
Theoretical pI: Translated: 7.94; Mature: 7.94
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.2 %Cys (Translated Protein) 1.9 %Met (Translated Protein) 2.1 %Cys+Met (Translated Protein) 0.2 %Cys (Mature Protein) 1.9 %Met (Mature Protein) 2.1 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKKKFYNILALSLALQTFVTPTHSFAESIEKTAELSIDEQALKSIEEHAKQHRHDEENDL CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCE EISGEYLLHLSTKLPLYDSNDLKTKTNIEIAEPVVKATKKKGNAYYIQTISGAGWVNKEN EECCEEEEEEEEECCCCCCCCCCEECCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCC NNLEVQEIYKLSNQKLIVTEETSVYSFPFQSFKEETKLQSQTVAPTEQAGNWFKIQMNGT CCCHHHHHHHHCCCEEEEEECCCEEECCHHHHHHHHHHHHHCCCCHHHCCCEEEEEECCC EKWIYAPSATFEGTKATLFEKKVSMENRSAAFFKTPSHLPTNNVYGVTFKEMIVPKGNEN EEEEECCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEEEEHHHEECCCCCCC IRPGYAMEPKYITIHETANTSKGANALNHAKYLDNQARGNTDRSASWHFTVDDKEIYQHL CCCCCEECCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEEECHHHHHHHC PLNEVGWHAGNKTGNYESIGIEIAVNSDGNYTKAVENARKLAAYLMNELNISLDHVQKHQ CCCHHCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH FWSKKNCPAFMIQRGQWDAFLKGTNAYYNEHHKEVMPPPEVPHEKDDITGGWYEQDIRQL HCCCCCCCEEEEECCCCCEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHH AAKKIMFGDGNGSYWPNRLVTRAEFASLISRSLKLPDGNAKFIDLNEAHPSLVDGINRAA HHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHH SAGIINGRGNNKFDPNATITRDEAVIMIDRALKYNWIYRKEVKLPFTDQHLAYDKKALQN HCCEEECCCCCCCCCCCEEECCCEEEEEECCCEEEEEEEEEECCCCCCHHHHHHHHHHHH VYAYGIVKGNERNEFVPKGTATRAEAAAFLNRMLKVIEA HHEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC >Mature Secondary Structure MKKKFYNILALSLALQTFVTPTHSFAESIEKTAELSIDEQALKSIEEHAKQHRHDEENDL CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCE EISGEYLLHLSTKLPLYDSNDLKTKTNIEIAEPVVKATKKKGNAYYIQTISGAGWVNKEN EECCEEEEEEEEECCCCCCCCCCEECCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCC NNLEVQEIYKLSNQKLIVTEETSVYSFPFQSFKEETKLQSQTVAPTEQAGNWFKIQMNGT CCCHHHHHHHHCCCEEEEEECCCEEECCHHHHHHHHHHHHHCCCCHHHCCCEEEEEECCC EKWIYAPSATFEGTKATLFEKKVSMENRSAAFFKTPSHLPTNNVYGVTFKEMIVPKGNEN EEEEECCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEEEEHHHEECCCCCCC IRPGYAMEPKYITIHETANTSKGANALNHAKYLDNQARGNTDRSASWHFTVDDKEIYQHL CCCCCEECCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEEECHHHHHHHC PLNEVGWHAGNKTGNYESIGIEIAVNSDGNYTKAVENARKLAAYLMNELNISLDHVQKHQ CCCHHCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH FWSKKNCPAFMIQRGQWDAFLKGTNAYYNEHHKEVMPPPEVPHEKDDITGGWYEQDIRQL HCCCCCCCEEEEECCCCCEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHH AAKKIMFGDGNGSYWPNRLVTRAEFASLISRSLKLPDGNAKFIDLNEAHPSLVDGINRAA HHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHH SAGIINGRGNNKFDPNATITRDEAVIMIDRALKYNWIYRKEVKLPFTDQHLAYDKKALQN HCCEEECCCCCCCCCCCEEECCCEEEEEECCCEEEEEEEEEECCCCCCHHHHHHHHHHHH VYAYGIVKGNERNEFVPKGTATRAEAAAFLNRMLKVIEA HHEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 8969508; 9384377 [H]