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The map label for this gene is nifS [H]

Identifier: 52144395

GI number: 52144395

Start: 957573

End: 958703

Strand: Reverse

Name: nifS [H]

Synonym: BCZK0830

Alternate gene names: 52144395

Gene position: 958703-957573 (Counterclockwise)

Preceding gene: 52144392

Following gene: 52144394

Centisome position: 18.09

GC content: 33.95

Gene sequence:

>1131_bases
GTGATCTCTATGCTTTATCTTGATAATAGTGCAACAACACAATTATTACCTGAAGTTAAAGAAGTAATGTTGCCATATTT
ACTTGAAGAATTCGGAAATCCTTCAAGTAAATATTACGGATTAGCCCAAAATGCTAAAGACGCTGTTGAAACAGCAAGAA
AACATGTAGCTCAATTATTAGGCTGTGAAACTGATGAAGTAATCTTTACTAGTGGTGCTACAGAAAGTAACAACTTTATT
TTGAAAGGCATCGCTCATTATCATAAAGATAAAGGCAATCACATTATTACTTCTAAAGTAGAACATCCTTCTATCATAGA
AACTTGTAAATTTTTAGAAGCAAATGGCTATACGGTCACATATCTTGATGTAGATCAATATGGCAGAATCAATTTGGAAG
AACTAACTAAAGCTATTCAACAAGAAAAAACTCTTCTCGTTTCAATTATGTGGGGCAACAACGAACTTGGTTCTCTAAAC
CAAATCAACGAGATCGCAAAAATCTGCAAAGAGAATGAAATATTCTTCCATACAGATGCTACACAAGTAGTTGGAAAAAT
ACAATTCTCTCTTTCAGATATAGACGGTATAAACTTTTTATCCTGCTCTTCTCATAAGTTTCATGGTCCAAAAGGTATTG
GGGTAACCATGATAGGTAAAGATAATTTAGGATTACCAATCCAAATCACTCCTCTACTTCACGGTGGCGGACAAGAACGT
GATTTACGCAGTGGCACACTACCCGTCCATAATATTGTTGGAATGGGAAAAGCTGCAGAAATCGCATATCTTAATTTGGA
ACAAAATATTTATAAATTAGAGCAACTAGAGAACAAATTAGCTGATATTCTAAAAACCAAATTTCAAAATCATATTGAGT
TTAACAATGATATTCAAGATAAAATACCTGGAATATTAAGTGTTATATTTAAAGGTGTAAACAACGAAACGCTTGTGAGG
ACTCTTGCTCCAGTAGCTGCCGTGTCTACTGGATCGGCTTGTAGCTCAAGTAAACCAAGTCATGTATTACAAGCTATCGG
ATTATCTATGGATGATTTGCGTTCTACTATTAGGTTCTCACTTTCTTCTACTACTAGTTTAGAAGAGTTGGATGTTTTTA
ATAATCTATAA

Upstream 100 bases:

>100_bases
ACGCTCACTTAGAACGCGGAATTAACTTATTAGACAGCGAATATAAACATGCTGGAAACAACGAGAAATTCACAATGAAC
TTGCTAAAATACGGTAAGGA

Downstream 100 bases:

>100_bases
AGAAAACAGAAAGGCATATCCCTTTCTGTTTTCTTTATTTCCTTACTAACTACTAATCTAGTAATTTTATATCTACGTTA
ATAGCTCCTCCCTACTTAAA

Product: cysteine desulphurase

Products: NA

Alternate protein names: NifS protein homolog [H]

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MISMLYLDNSATTQLLPEVKEVMLPYLLEEFGNPSSKYYGLAQNAKDAVETARKHVAQLLGCETDEVIFTSGATESNNFI
LKGIAHYHKDKGNHIITSKVEHPSIIETCKFLEANGYTVTYLDVDQYGRINLEELTKAIQQEKTLLVSIMWGNNELGSLN
QINEIAKICKENEIFFHTDATQVVGKIQFSLSDIDGINFLSCSSHKFHGPKGIGVTMIGKDNLGLPIQITPLLHGGGQER
DLRSGTLPVHNIVGMGKAAEIAYLNLEQNIYKLEQLENKLADILKTKFQNHIEFNNDIQDKIPGILSVIFKGVNNETLVR
TLAPVAAVSTGSACSSSKPSHVLQAIGLSMDDLRSTIRFSLSSTTSLEELDVFNNL

Sequences:

>Translated_376_residues
MISMLYLDNSATTQLLPEVKEVMLPYLLEEFGNPSSKYYGLAQNAKDAVETARKHVAQLLGCETDEVIFTSGATESNNFI
LKGIAHYHKDKGNHIITSKVEHPSIIETCKFLEANGYTVTYLDVDQYGRINLEELTKAIQQEKTLLVSIMWGNNELGSLN
QINEIAKICKENEIFFHTDATQVVGKIQFSLSDIDGINFLSCSSHKFHGPKGIGVTMIGKDNLGLPIQITPLLHGGGQER
DLRSGTLPVHNIVGMGKAAEIAYLNLEQNIYKLEQLENKLADILKTKFQNHIEFNNDIQDKIPGILSVIFKGVNNETLVR
TLAPVAAVSTGSACSSSKPSHVLQAIGLSMDDLRSTIRFSLSSTTSLEELDVFNNL
>Mature_376_residues
MISMLYLDNSATTQLLPEVKEVMLPYLLEEFGNPSSKYYGLAQNAKDAVETARKHVAQLLGCETDEVIFTSGATESNNFI
LKGIAHYHKDKGNHIITSKVEHPSIIETCKFLEANGYTVTYLDVDQYGRINLEELTKAIQQEKTLLVSIMWGNNELGSLN
QINEIAKICKENEIFFHTDATQVVGKIQFSLSDIDGINFLSCSSHKFHGPKGIGVTMIGKDNLGLPIQITPLLHGGGQER
DLRSGTLPVHNIVGMGKAAEIAYLNLEQNIYKLEQLENKLADILKTKFQNHIEFNNDIQDKIPGILSVIFKGVNNETLVR
TLAPVAAVSTGSACSSSKPSHVLQAIGLSMDDLRSTIRFSLSSTTSLEELDVFNNL

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=370, Percent_Identity=42.1621621621622, Blast_Score=295, Evalue=6e-80,
Organism=Homo sapiens, GI156713448, Length=404, Percent_Identity=33.1683168316832, Blast_Score=188, Evalue=9e-48,
Organism=Escherichia coli, GI48994898, Length=371, Percent_Identity=43.1266846361186, Blast_Score=296, Evalue=1e-81,
Organism=Escherichia coli, GI1789175, Length=400, Percent_Identity=26, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787970, Length=324, Percent_Identity=25.9259259259259, Blast_Score=78, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI25143064, Length=369, Percent_Identity=40.9214092140921, Blast_Score=285, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI17533177, Length=321, Percent_Identity=31.4641744548287, Blast_Score=134, Evalue=8e-32,
Organism=Saccharomyces cerevisiae, GI6319831, Length=369, Percent_Identity=40.1084010840108, Blast_Score=268, Evalue=7e-73,
Organism=Drosophila melanogaster, GI20129463, Length=369, Percent_Identity=41.7344173441734, Blast_Score=289, Evalue=2e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR010240
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 41469; Mature: 41469

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISMLYLDNSATTQLLPEVKEVMLPYLLEEFGNPSSKYYGLAQNAKDAVETARKHVAQLL
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHEEECCCCHHHHHHHHHHHHHHHH
GCETDEVIFTSGATESNNFILKGIAHYHKDKGNHIITSKVEHPSIIETCKFLEANGYTVT
CCCCCCEEEECCCCCCCCEEEEEHHHHHCCCCCEEEEECCCCCHHHHHHHHEECCCCEEE
YLDVDQYGRINLEELTKAIQQEKTLLVSIMWGNNELGSLNQINEIAKICKENEIFFHTDA
EEECCCCCCCCHHHHHHHHHHCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCH
TQVVGKIQFSLSDIDGINFLSCSSHKFHGPKGIGVTMIGKDNLGLPIQITPLLHGGGQER
HHHHHHHEEEEECCCCCEEEECCCCCCCCCCCCEEEEECCCCCCCCEEEEEEECCCCCCC
DLRSGTLPVHNIVGMGKAAEIAYLNLEQNIYKLEQLENKLADILKTKFQNHIEFNNDIQD
CCCCCCCCHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
KIPGILSVIFKGVNNETLVRTLAPVAAVSTGSACSSSKPSHVLQAIGLSMDDLRSTIRFS
HHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHH
LSSTTSLEELDVFNNL
CCCCCCHHHHHHHCCC
>Mature Secondary Structure
MISMLYLDNSATTQLLPEVKEVMLPYLLEEFGNPSSKYYGLAQNAKDAVETARKHVAQLL
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHEEECCCCHHHHHHHHHHHHHHHH
GCETDEVIFTSGATESNNFILKGIAHYHKDKGNHIITSKVEHPSIIETCKFLEANGYTVT
CCCCCCEEEECCCCCCCCEEEEEHHHHHCCCCCEEEEECCCCCHHHHHHHHEECCCCEEE
YLDVDQYGRINLEELTKAIQQEKTLLVSIMWGNNELGSLNQINEIAKICKENEIFFHTDA
EEECCCCCCCCHHHHHHHHHHCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCH
TQVVGKIQFSLSDIDGINFLSCSSHKFHGPKGIGVTMIGKDNLGLPIQITPLLHGGGQER
HHHHHHHEEEEECCCCCEEEECCCCCCCCCCCCEEEEECCCCCCCCEEEEEEECCCCCCC
DLRSGTLPVHNIVGMGKAAEIAYLNLEQNIYKLEQLENKLADILKTKFQNHIEFNNDIQD
CCCCCCCCHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
KIPGILSVIFKGVNNETLVRTLAPVAAVSTGSACSSSKPSHVLQAIGLSMDDLRSTIRFS
HHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHH
LSSTTSLEELDVFNNL
CCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10930739 [H]