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The map label for this gene is dhaL [H]

Identifier: 52144357

GI number: 52144357

Start: 991135

End: 992133

Strand: Reverse

Name: dhaL [H]

Synonym: BCZK0868

Alternate gene names: 52144357

Gene position: 992133-991135 (Counterclockwise)

Preceding gene: 52144355

Following gene: 52144361

Centisome position: 18.72

GC content: 38.54

Gene sequence:

>999_bases
ATGAAAAAGATTATGAATGATGTACAAAATATCGTTCAAGATATGATGCATGGCTTTTATTTTGAACATAACGACAAAGT
AAATTACGACGAAACAAATAACATCATTTATGTAAAAGATATTGAAAAACTAAAGCAAAACGTCGCTATTATAAGCGGTG
GTGGTAGTGGACACGAACCAGCTGATATTGGTTATGTAGGAAAAGGAATGCTTACAGCTGCAGTAAACGGCAGCATCTTC
ACTCCGCCTACAGTAGAACAAATCGTAATGGCAACTCGCCTCATGCCAAAAGATAAAAGTATACTATTCATCATCAAAAA
CTTTAAAGATGACGTAGAAAACTTTCTAGCAGCAGAACAAATCGCCAAAGGCGAAGGAAGAAAAATTGACCATATCATCG
TAAATGACGATGTTTCAATTGAAGATGATGCTTCTTTTAATAAAAGAAGACGCGGCGTCGCTGGTACTGTTTTCGTTCAA
AAAATATTAGGTTCGGCCGCTCTTGAAGGTCATTCTTTAGAAGAACTGACTGCACTCGGCCGCTCTATCACCGAAAACTT
ACACACATTAGGAGTTGCTCTTTCACCTGCAAACGATCCAGTGAAAGGAAAAGCTTCCTTCACATTAAACGATGATGAAG
TCTTTTACGGCGTCGGTATTCACGGAGAAAAAGGTTACCGTAAAGAAGCACTATCCTCTTCTGAAATATTAGCGATTGAA
CTAATGAACAAATTAAAAAGCATTTACCGCTGGAGAAAAGGTGACAACTTTGCGATTCTCATTAATGGATTAGGTGCGAC
ACCATTAATGGAACAATACATTTTCGCAAATGACATTCGTCGTTTATGTGAACTGGAAGGATTACATGTGAAGTTCGTAA
AGGTTGGTACGCTGTTGACTTCTTTAGATATGAAAGGTGTTTCACTCAGTTTGCTTAAGGTAGAGGATTTGGATTGGGTG
AAGTGGTTGAAGGCGGATGCTCGAGTGGGTAGTTGGTAA

Upstream 100 bases:

>100_bases
GATCCTTCCATTATGTCTTCTCTCATGAAAAATATGATAAACAATCAATTACTACTATTACTGGAGCAATCAGCAAAGTA
ATTAAAGGGTGGCAAAAGCA

Downstream 100 bases:

>100_bases
GTAAAAATTATGATGATAAAAGCCTTTAACTATAACAAGTTAAAGGCTTTTTGCACATTGAAATATCGCACGAGCGTTCA
AAAGAAATTCGTAATCACCT

Product: DhaKLM operon coactivator DhaQ

Products: ADP; glycerone phosphate

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MKKIMNDVQNIVQDMMHGFYFEHNDKVNYDETNNIIYVKDIEKLKQNVAIISGGGSGHEPADIGYVGKGMLTAAVNGSIF
TPPTVEQIVMATRLMPKDKSILFIIKNFKDDVENFLAAEQIAKGEGRKIDHIIVNDDVSIEDDASFNKRRRGVAGTVFVQ
KILGSAALEGHSLEELTALGRSITENLHTLGVALSPANDPVKGKASFTLNDDEVFYGVGIHGEKGYRKEALSSSEILAIE
LMNKLKSIYRWRKGDNFAILINGLGATPLMEQYIFANDIRRLCELEGLHVKFVKVGTLLTSLDMKGVSLSLLKVEDLDWV
KWLKADARVGSW

Sequences:

>Translated_332_residues
MKKIMNDVQNIVQDMMHGFYFEHNDKVNYDETNNIIYVKDIEKLKQNVAIISGGGSGHEPADIGYVGKGMLTAAVNGSIF
TPPTVEQIVMATRLMPKDKSILFIIKNFKDDVENFLAAEQIAKGEGRKIDHIIVNDDVSIEDDASFNKRRRGVAGTVFVQ
KILGSAALEGHSLEELTALGRSITENLHTLGVALSPANDPVKGKASFTLNDDEVFYGVGIHGEKGYRKEALSSSEILAIE
LMNKLKSIYRWRKGDNFAILINGLGATPLMEQYIFANDIRRLCELEGLHVKFVKVGTLLTSLDMKGVSLSLLKVEDLDWV
KWLKADARVGSW
>Mature_332_residues
MKKIMNDVQNIVQDMMHGFYFEHNDKVNYDETNNIIYVKDIEKLKQNVAIISGGGSGHEPADIGYVGKGMLTAAVNGSIF
TPPTVEQIVMATRLMPKDKSILFIIKNFKDDVENFLAAEQIAKGEGRKIDHIIVNDDVSIEDDASFNKRRRGVAGTVFVQ
KILGSAALEGHSLEELTALGRSITENLHTLGVALSPANDPVKGKASFTLNDDEVFYGVGIHGEKGYRKEALSSSEILAIE
LMNKLKSIYRWRKGDNFAILINGLGATPLMEQYIFANDIRRLCELEGLHVKFVKVGTLLTSLDMKGVSLSLLKVEDLDWV
KWLKADARVGSW

Specific function: Dihydroxyacetone binding subunit of the dihydroxyacetone kinase, which is responsible for phosphorylating dihydroxyacetone [H]

COG id: COG2376

COG function: function code G; Dihydroxyacetone kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DhaK domain [H]

Homologues:

Organism=Homo sapiens, GI20149621, Length=340, Percent_Identity=32.0588235294118, Blast_Score=184, Evalue=1e-46,
Organism=Escherichia coli, GI87081857, Length=347, Percent_Identity=35.7348703170029, Blast_Score=221, Evalue=3e-59,
Organism=Caenorhabditis elegans, GI17565018, Length=336, Percent_Identity=34.2261904761905, Blast_Score=174, Evalue=4e-44,
Organism=Saccharomyces cerevisiae, GI6321055, Length=295, Percent_Identity=30.1694915254237, Blast_Score=130, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6323570, Length=324, Percent_Identity=31.1728395061728, Blast_Score=130, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004006
- InterPro:   IPR012736 [H]

Pfam domain/function: PF02733 Dak1 [H]

EC number: 2.7.1.29

Molecular weight: Translated: 36815; Mature: 36815

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIMNDVQNIVQDMMHGFYFEHNDKVNYDETNNIIYVKDIEKLKQNVAIISGGGSGHEP
CHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCEEEEECHHHHHCCEEEEECCCCCCCC
ADIGYVGKGMLTAAVNGSIFTPPTVEQIVMATRLMPKDKSILFIIKNFKDDVENFLAAEQ
CCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHH
IAKGEGRKIDHIIVNDDVSIEDDASFNKRRRGVAGTVFVQKILGSAALEGHSLEELTALG
HHCCCCCEEEEEEECCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHH
RSITENLHTLGVALSPANDPVKGKASFTLNDDEVFYGVGIHGEKGYRKEALSSSEILAIE
HHHHHHHHHHEEEECCCCCCCCCCEEEEECCCCEEEEEEECCCCCCHHHHCCCCCCHHHH
LMNKLKSIYRWRKGDNFAILINGLGATPLMEQYIFANDIRRLCELEGLHVKFVKVGTLLT
HHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHH
SLDMKGVSLSLLKVEDLDWVKWLKADARVGSW
HCCCCCCEEEEEEECCCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKKIMNDVQNIVQDMMHGFYFEHNDKVNYDETNNIIYVKDIEKLKQNVAIISGGGSGHEP
CHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCEEEEECHHHHHCCEEEEECCCCCCCC
ADIGYVGKGMLTAAVNGSIFTPPTVEQIVMATRLMPKDKSILFIIKNFKDDVENFLAAEQ
CCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHH
IAKGEGRKIDHIIVNDDVSIEDDASFNKRRRGVAGTVFVQKILGSAALEGHSLEELTALG
HHCCCCCEEEEEEECCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHH
RSITENLHTLGVALSPANDPVKGKASFTLNDDEVFYGVGIHGEKGYRKEALSSSEILAIE
HHHHHHHHHHEEEECCCCCCCCCCEEEEECCCCEEEEEEECCCCCCHHHHCCCCCCHHHH
LMNKLKSIYRWRKGDNFAILINGLGATPLMEQYIFANDIRRLCELEGLHVKFVKVGTLLT
HHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHH
SLDMKGVSLSLLKVEDLDWVKWLKADARVGSW
HCCCCCCEEEEEEECCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; glycerone

Specific reaction: ATP + glycerone = ADP + glycerone phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]