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The map label for this gene is asnO [H]

Identifier: 52144174

GI number: 52144174

Start: 1158071

End: 1159849

Strand: Direct

Name: asnO [H]

Synonym: BCZK1053

Alternate gene names: 52144174

Gene position: 1158071-1159849 (Clockwise)

Preceding gene: 52144176

Following gene: 52144173

Centisome position: 21.85

GC content: 38.28

Gene sequence:

>1779_bases
ATGGCTGAGACGTTAGCGAAGCGTGGCCCAGATGATAATAAAGTTTGGATTAAAGGCAATGTCGCATTTGGGCATAAACG
GTTAATCGTTGTTGATCCTGAAGGTGGTAAACAGCCGATGACTTGTTTAAAGGATGAAGCAAATTATGCAATTTGCTATA
ACGGCGAACTGTATAACACGGAAGACATTCGAAAGGAACTATTAAGAAGAGGATATACGTTCAAAGGTCACTCTGATACG
GAAGTATTGTTAGCTTCTTATATCGAATGGAAAGAAGAATGTGTCGATCATTTAAACGGTATATATGCGTTTGCTGTATG
GGATGAACAGAAAGAACAAGTGTTTATTGCGCGCGATCGATTAGGGGTAAAACCACTATTTTATAAATATGACAGTGGGC
GATTACTATTTGGCTCAGAGTTAAAAGCGATATTGGCGCATCCAGATGTGAAGGCGGAAGTGACGTTAGAAGGATTATCT
GAAATATTTGGCCTTGGACCATCTAGAACGCCTGGTCACGGTATTTATGCTGGTATAAAAGAATTGCGTCCAGGTCACGC
GATGACATTTTCGAAGAACGGTTTATGTATATGGAGATATTGGAATGTAGAAAGTAAAAAGCATGAAGATTCCTTTGAAG
AAACAGTAGAAAAAACACGCTTTTTATTACAAGATGCGATTACAAGGCAGCTCGTTTCGGATGTACCATTATGCACTTTT
TTATCAGGCGGTGTAGATTCAAGCGCTATAACAGCAATTGCTGCGAAAGAATACGAAAGATCGGGAAAAGGTCAATTACA
CACGTATTCGGTTGATTACGAAGATAATGACAAATACTTTAAAGCAAATGCGTTTCAGCCAAATTCAGATGCACCATTTA
TTCATTTGATGACGGAGACGTTTCAAACGATCCATCATCGCTGTGTCATTTCAAATGAACAATTAGCGCAGTATTTAACA
GAAGCAGTACTCGTTCGCGATTTGCCTGGTATGGCAGATATCGATTCGTCATTATTATGGTTTTGTCGTGAGATTAAACA
AGATTTTGTCGTCGGTCTATCTGGGGAATGTGCAGATGAAATATTTGGTGGTTATCCGTGGTTTTATAGAGAAGATGATT
TACAATCGAGCGCATTTCCGTGGATGCGTTCTACAGAAGCACGTGAACAATTGCTAAAGAAAGAATGGAGAAATAAATTA
AATTTACAACAATATGTACAGCAGCGCTATGAAGAGTCCATTCAAGAAGTTCCTATTTTAGATGGGGAAAGTCCGCTAGA
AGCAAAAAGACGCCAATTATTTTATTTAAATATGGTATGGTTTATGACAACATTATTAGACAGAAAAGACCGCATGAGTA
TGGGGGCGAGCTTAGAAGTACGTGTTCCATTTGCAGATCATCGTCTTGTCGAATATGCGTGGAATATTCCTTGGGAAATG
AAAATGTATAAAAACCGCGAAAAAGGTCTATTGCGTAAAGCGTTAGAAGGTTTACTTCCAAATGAGATATTATATAGAAA
GAAGAGCCCGTATCCAAAAACACATAATCCGCACTATACGAAAGCAGTAACAGTATGGATACAAGATTTATTAACAGATA
AAGGCTCAATTTTACACGAGCTATTTGATAAAGAACAGCTGAGTGGATTAATTGAGTCAGGCGGCAGTGCATTCCAAACG
CCATGGTATGGTCAATTAATGACTGGGCCACAACTTTTAGCTCATTTAGCACAAATTCACGTTTGGTTTAAAGAATATGG
AGTAAATATTAAAGAATAG

Upstream 100 bases:

>100_bases
AGAAAAGAAATAAGATGGAAGGATGATTTATATGTGCGGGATTACAGGATGGGTGGATTATAAACGCTCATTAGAAGGAG
AACGGGACGTCGTTACGAAG

Downstream 100 bases:

>100_bases
GAGAAAAGCGACTTACTTTATAAGTAAGTCGCTTTTTATTAAATGAATTGTCATATAAAATTCAAAATCATATTAGAAAA
AATACTTATTTAGAATAATT

Product: asparagine synthetase, glutamine-hydrolyzing

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 592; Mature: 591

Protein sequence:

>592_residues
MAETLAKRGPDDNKVWIKGNVAFGHKRLIVVDPEGGKQPMTCLKDEANYAICYNGELYNTEDIRKELLRRGYTFKGHSDT
EVLLASYIEWKEECVDHLNGIYAFAVWDEQKEQVFIARDRLGVKPLFYKYDSGRLLFGSELKAILAHPDVKAEVTLEGLS
EIFGLGPSRTPGHGIYAGIKELRPGHAMTFSKNGLCIWRYWNVESKKHEDSFEETVEKTRFLLQDAITRQLVSDVPLCTF
LSGGVDSSAITAIAAKEYERSGKGQLHTYSVDYEDNDKYFKANAFQPNSDAPFIHLMTETFQTIHHRCVISNEQLAQYLT
EAVLVRDLPGMADIDSSLLWFCREIKQDFVVGLSGECADEIFGGYPWFYREDDLQSSAFPWMRSTEAREQLLKKEWRNKL
NLQQYVQQRYEESIQEVPILDGESPLEAKRRQLFYLNMVWFMTTLLDRKDRMSMGASLEVRVPFADHRLVEYAWNIPWEM
KMYKNREKGLLRKALEGLLPNEILYRKKSPYPKTHNPHYTKAVTVWIQDLLTDKGSILHELFDKEQLSGLIESGGSAFQT
PWYGQLMTGPQLLAHLAQIHVWFKEYGVNIKE

Sequences:

>Translated_592_residues
MAETLAKRGPDDNKVWIKGNVAFGHKRLIVVDPEGGKQPMTCLKDEANYAICYNGELYNTEDIRKELLRRGYTFKGHSDT
EVLLASYIEWKEECVDHLNGIYAFAVWDEQKEQVFIARDRLGVKPLFYKYDSGRLLFGSELKAILAHPDVKAEVTLEGLS
EIFGLGPSRTPGHGIYAGIKELRPGHAMTFSKNGLCIWRYWNVESKKHEDSFEETVEKTRFLLQDAITRQLVSDVPLCTF
LSGGVDSSAITAIAAKEYERSGKGQLHTYSVDYEDNDKYFKANAFQPNSDAPFIHLMTETFQTIHHRCVISNEQLAQYLT
EAVLVRDLPGMADIDSSLLWFCREIKQDFVVGLSGECADEIFGGYPWFYREDDLQSSAFPWMRSTEAREQLLKKEWRNKL
NLQQYVQQRYEESIQEVPILDGESPLEAKRRQLFYLNMVWFMTTLLDRKDRMSMGASLEVRVPFADHRLVEYAWNIPWEM
KMYKNREKGLLRKALEGLLPNEILYRKKSPYPKTHNPHYTKAVTVWIQDLLTDKGSILHELFDKEQLSGLIESGGSAFQT
PWYGQLMTGPQLLAHLAQIHVWFKEYGVNIKE
>Mature_591_residues
AETLAKRGPDDNKVWIKGNVAFGHKRLIVVDPEGGKQPMTCLKDEANYAICYNGELYNTEDIRKELLRRGYTFKGHSDTE
VLLASYIEWKEECVDHLNGIYAFAVWDEQKEQVFIARDRLGVKPLFYKYDSGRLLFGSELKAILAHPDVKAEVTLEGLSE
IFGLGPSRTPGHGIYAGIKELRPGHAMTFSKNGLCIWRYWNVESKKHEDSFEETVEKTRFLLQDAITRQLVSDVPLCTFL
SGGVDSSAITAIAAKEYERSGKGQLHTYSVDYEDNDKYFKANAFQPNSDAPFIHLMTETFQTIHHRCVISNEQLAQYLTE
AVLVRDLPGMADIDSSLLWFCREIKQDFVVGLSGECADEIFGGYPWFYREDDLQSSAFPWMRSTEAREQLLKKEWRNKLN
LQQYVQQRYEESIQEVPILDGESPLEAKRRQLFYLNMVWFMTTLLDRKDRMSMGASLEVRVPFADHRLVEYAWNIPWEMK
MYKNREKGLLRKALEGLLPNEILYRKKSPYPKTHNPHYTKAVTVWIQDLLTDKGSILHELFDKEQLSGLIESGGSAFQTP
WYGQLMTGPQLLAHLAQIHVWFKEYGVNIKE

Specific function: Asparagine synthetase involved in sporulation [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=528, Percent_Identity=26.7045454545455, Blast_Score=139, Evalue=8e-33,
Organism=Homo sapiens, GI168229252, Length=528, Percent_Identity=26.7045454545455, Blast_Score=139, Evalue=8e-33,
Organism=Homo sapiens, GI168229250, Length=528, Percent_Identity=26.7045454545455, Blast_Score=139, Evalue=8e-33,
Organism=Homo sapiens, GI296010848, Length=525, Percent_Identity=26.6666666666667, Blast_Score=139, Evalue=1e-32,
Organism=Homo sapiens, GI296010852, Length=470, Percent_Identity=24.2553191489362, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI296010850, Length=470, Percent_Identity=24.2553191489362, Blast_Score=105, Evalue=1e-22,
Organism=Escherichia coli, GI1786889, Length=534, Percent_Identity=26.0299625468165, Blast_Score=150, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17560178, Length=603, Percent_Identity=25.7048092868988, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI71993933, Length=551, Percent_Identity=26.1343012704174, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI25147557, Length=551, Percent_Identity=25.7713248638838, Blast_Score=123, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6325403, Length=547, Percent_Identity=25.0457038391225, Blast_Score=129, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6321563, Length=546, Percent_Identity=24.9084249084249, Blast_Score=128, Evalue=2e-30,
Organism=Drosophila melanogaster, GI45553209, Length=524, Percent_Identity=26.1450381679389, Blast_Score=130, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 68327; Mature: 68195

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAETLAKRGPDDNKVWIKGNVAFGHKRLIVVDPEGGKQPMTCLKDEANYAICYNGELYNT
CCCCHHHCCCCCCEEEEEEEEEECCEEEEEECCCCCCCCHHHHHCCCCEEEEECCCEECH
EDIRKELLRRGYTFKGHSDTEVLLASYIEWKEECVDHLNGIYAFAVWDEQKEQVFIARDR
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEECCC
LGVKPLFYKYDSGRLLFGSELKAILAHPDVKAEVTLEGLSEIFGLGPSRTPGHGIYAGIK
CCCCEEEEEECCCCEEECCHHHHHHCCCCCCEEEEHHHHHHHHCCCCCCCCCCHHHHHHH
ELRPGHAMTFSKNGLCIWRYWNVESKKHEDSFEETVEKTRFLLQDAITRQLVSDVPLCTF
HCCCCCEEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
LSGGVDSSAITAIAAKEYERSGKGQLHTYSVDYEDNDKYFKANAFQPNSDAPFIHLMTET
HCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEECCCCCCCCCCEEEHHHHH
FQTIHHRCVISNEQLAQYLTEAVLVRDLPGMADIDSSLLWFCREIKQDFVVGLSGECADE
HHHHHHHHEECHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHEECCCCCHHHH
IFGGYPWFYREDDLQSSAFPWMRSTEAREQLLKKEWRNKLNLQQYVQQRYEESIQEVPIL
HHCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
DGESPLEAKRRQLFYLNMVWFMTTLLDRKDRMSMGASLEVRVPFADHRLVEYAWNIPWEM
CCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEEECCCCCCHHHHHHHCCCEEE
KMYKNREKGLLRKALEGLLPNEILYRKKSPYPKTHNPHYTKAVTVWIQDLLTDKGSILHE
HHHHCHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHH
LFDKEQLSGLIESGGSAFQTPWYGQLMTGPQLLAHLAQIHVWFKEYGVNIKE
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
AETLAKRGPDDNKVWIKGNVAFGHKRLIVVDPEGGKQPMTCLKDEANYAICYNGELYNT
CCCHHHCCCCCCEEEEEEEEEECCEEEEEECCCCCCCCHHHHHCCCCEEEEECCCEECH
EDIRKELLRRGYTFKGHSDTEVLLASYIEWKEECVDHLNGIYAFAVWDEQKEQVFIARDR
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEECCC
LGVKPLFYKYDSGRLLFGSELKAILAHPDVKAEVTLEGLSEIFGLGPSRTPGHGIYAGIK
CCCCEEEEEECCCCEEECCHHHHHHCCCCCCEEEEHHHHHHHHCCCCCCCCCCHHHHHHH
ELRPGHAMTFSKNGLCIWRYWNVESKKHEDSFEETVEKTRFLLQDAITRQLVSDVPLCTF
HCCCCCEEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
LSGGVDSSAITAIAAKEYERSGKGQLHTYSVDYEDNDKYFKANAFQPNSDAPFIHLMTET
HCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEECCCCCCCCCCEEEHHHHH
FQTIHHRCVISNEQLAQYLTEAVLVRDLPGMADIDSSLLWFCREIKQDFVVGLSGECADE
HHHHHHHHEECHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHEECCCCCHHHH
IFGGYPWFYREDDLQSSAFPWMRSTEAREQLLKKEWRNKLNLQQYVQQRYEESIQEVPIL
HHCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
DGESPLEAKRRQLFYLNMVWFMTTLLDRKDRMSMGASLEVRVPFADHRLVEYAWNIPWEM
CCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEEECCCCCCHHHHHHHCCCEEE
KMYKNREKGLLRKALEGLLPNEILYRKKSPYPKTHNPHYTKAVTVWIQDLLTDKGSILHE
HHHHCHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHH
LFDKEQLSGLIESGGSAFQTPWYGQLMTGPQLLAHLAQIHVWFKEYGVNIKE
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377; 10498721 [H]