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The map label for this gene is cls [H]

Identifier: 52144139

GI number: 52144139

Start: 1197257

End: 1198801

Strand: Direct

Name: cls [H]

Synonym: BCZK1088

Alternate gene names: 52144139

Gene position: 1197257-1198801 (Clockwise)

Preceding gene: 52144141

Following gene: 52144138

Centisome position: 22.59

GC content: 35.47

Gene sequence:

>1545_bases
ATGAAAAATACGTTAAAGTTGATTTTCTTTGTTTTACTATTGTTCGCATTATTTGTTTCATTACGTATGTTTATTGATGT
GGCATTTTATTCGGATGTAATTGGGATAAAAGATGTTTCAATTTTAGGTATTATTAGTATTTTATTTACGGTATCTGCAT
TTTTAATTGGCTGTGTTATTTTCTTAGAGAACCGTCATCCGTCAAAAACACTTACATGGCTCATTGTATTAGGTATTTTT
CCCGTATTTGGATTCTTCGCTTATTTATTATTCGGACAAAACTTCCGGAGGAAGAGAATGTTCCAAAAGAAGGCGTTGCT
CGATGAACAAGCGTTTTTACAATATAAGGGACATGAAGATTACGAAGAACGGATTTTGCGCAATCATAAGCATCAAGAGC
TATTATTTCGTTTAGCGGATCGCTTAGGCGCTTTAAATATTTCATTTCAAACTGAAACGAGAACATTAACAAATGGAGAT
GAAACGTTTCAGGCCATTTTAGATGGATTAAAACGAGCGAAACATCACATTCATATGGAATATTACATTGTGCGTGATGA
TAAGCTTGGAACAGAAATTAAAGATATTTTAATACAAAAATCAAAAGAGGGCGTAGTTGTTCGTTTTTTATATGATGCGG
TTGGAAGTTTTAAATTATCAAAATCGTATATTGAAGAATTAAACGATGCAGGTGTCGAAGTAATTCCGTTCTTCCCTGTG
CGCTTTCCAATTTTAAACGATAAGATTAATTATCGAAACCACCGAAAAATCGTTATTATTGATGGAAATGAAGGGTTTGT
AGGTGGATTAAATATTGGCGATGAATATTTAGGGAAGGATAAGTACTTCGGCTTTTGGCGAGACACACATTTATATTTGC
GTGGTGAAGCTGTACAAAGTTTGCAACTTATTTTCCTTCAAGATTGGTTTTATATGACTGGTGAGGCTGTGCTAGCCCCT
GAATATTTACAAGCGAAAGCAGTTGAGGGAGAGCATTGGGGAGGCGTGCAGCTCGTTGCAGGTGGGCCGGATAATAAATG
GGAAACAATCAAACATTTATATTTTGCTATGATTGCTTCTGCGCGGAAATCCATTTGGATTGCAACGCCGTATTTCATTC
CGGATGATGATATTTTATCTGCATTAAAGGTAGCTGCGCTTGCTGGTATCGATGTTCGTTTGTTAATGCCGAGTAAGCCT
GATAAGCGCACTGTCTTTTACGCATCGAGATCGTATTTCCCAGAGCTTTTAGATGCAGGGGTAAAGATATATGAATACGA
GAAAGGTTTTCTCCATAGTAAAGTTGTCATTGTCGATTCTGATTTAGCTTCAATTGGGACAGCGAATATGGATATGAGAA
GTTTTCATTTGAACTTTGAAGTAAATGCCTTTTTATATGATACAGATAGCATTCGAAAACTTGTTCAAGATTTTAAAGAC
GATTTAGAAGAATCAAGTGAAATTCATGTCGATCGTTTTCATAAAAGGCGTCTTCATAGACGAATTGTTGAATCAACGTA
CCGATTATTATCACCTTTATTATAA

Upstream 100 bases:

>100_bases
TCGCAATAATTTTCCTGCTAAAACGTAGGCCGATTTCTGTAGTATGAAATCGGCTTTTTATATGAAGAATAGGAAAGCTT
TCTTAGTAAGGAGGTAAGAG

Downstream 100 bases:

>100_bases
AGAGAGATGTCCGAAATTGGGACATCTCTTTTTTAAAAGGAATTTGTAATAAATTGTAGAATATAAGGAGCTGAAAGGGT
GTGATTCCGATATTTATCGC

Product: cardiolipin synthetase

Products: NA

Alternate protein names: CL synthase 2 [H]

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MKNTLKLIFFVLLLFALFVSLRMFIDVAFYSDVIGIKDVSILGIISILFTVSAFLIGCVIFLENRHPSKTLTWLIVLGIF
PVFGFFAYLLFGQNFRRKRMFQKKALLDEQAFLQYKGHEDYEERILRNHKHQELLFRLADRLGALNISFQTETRTLTNGD
ETFQAILDGLKRAKHHIHMEYYIVRDDKLGTEIKDILIQKSKEGVVVRFLYDAVGSFKLSKSYIEELNDAGVEVIPFFPV
RFPILNDKINYRNHRKIVIIDGNEGFVGGLNIGDEYLGKDKYFGFWRDTHLYLRGEAVQSLQLIFLQDWFYMTGEAVLAP
EYLQAKAVEGEHWGGVQLVAGGPDNKWETIKHLYFAMIASARKSIWIATPYFIPDDDILSALKVAALAGIDVRLLMPSKP
DKRTVFYASRSYFPELLDAGVKIYEYEKGFLHSKVVIVDSDLASIGTANMDMRSFHLNFEVNAFLYDTDSIRKLVQDFKD
DLEESSEIHVDRFHKRRLHRRIVESTYRLLSPLL

Sequences:

>Translated_514_residues
MKNTLKLIFFVLLLFALFVSLRMFIDVAFYSDVIGIKDVSILGIISILFTVSAFLIGCVIFLENRHPSKTLTWLIVLGIF
PVFGFFAYLLFGQNFRRKRMFQKKALLDEQAFLQYKGHEDYEERILRNHKHQELLFRLADRLGALNISFQTETRTLTNGD
ETFQAILDGLKRAKHHIHMEYYIVRDDKLGTEIKDILIQKSKEGVVVRFLYDAVGSFKLSKSYIEELNDAGVEVIPFFPV
RFPILNDKINYRNHRKIVIIDGNEGFVGGLNIGDEYLGKDKYFGFWRDTHLYLRGEAVQSLQLIFLQDWFYMTGEAVLAP
EYLQAKAVEGEHWGGVQLVAGGPDNKWETIKHLYFAMIASARKSIWIATPYFIPDDDILSALKVAALAGIDVRLLMPSKP
DKRTVFYASRSYFPELLDAGVKIYEYEKGFLHSKVVIVDSDLASIGTANMDMRSFHLNFEVNAFLYDTDSIRKLVQDFKD
DLEESSEIHVDRFHKRRLHRRIVESTYRLLSPLL
>Mature_514_residues
MKNTLKLIFFVLLLFALFVSLRMFIDVAFYSDVIGIKDVSILGIISILFTVSAFLIGCVIFLENRHPSKTLTWLIVLGIF
PVFGFFAYLLFGQNFRRKRMFQKKALLDEQAFLQYKGHEDYEERILRNHKHQELLFRLADRLGALNISFQTETRTLTNGD
ETFQAILDGLKRAKHHIHMEYYIVRDDKLGTEIKDILIQKSKEGVVVRFLYDAVGSFKLSKSYIEELNDAGVEVIPFFPV
RFPILNDKINYRNHRKIVIIDGNEGFVGGLNIGDEYLGKDKYFGFWRDTHLYLRGEAVQSLQLIFLQDWFYMTGEAVLAP
EYLQAKAVEGEHWGGVQLVAGGPDNKWETIKHLYFAMIASARKSIWIATPYFIPDDDILSALKVAALAGIDVRLLMPSKP
DKRTVFYASRSYFPELLDAGVKIYEYEKGFLHSKVVIVDSDLASIGTANMDMRSFHLNFEVNAFLYDTDSIRKLVQDFKD
DLEESSEIHVDRFHKRRLHRRIVESTYRLLSPLL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=464, Percent_Identity=30.1724137931034, Blast_Score=230, Evalue=1e-61,
Organism=Escherichia coli, GI1787007, Length=334, Percent_Identity=29.940119760479, Blast_Score=140, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 59470; Mature: 59470

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNTLKLIFFVLLLFALFVSLRMFIDVAFYSDVIGIKDVSILGIISILFTVSAFLIGCVI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
FLENRHPSKTLTWLIVLGIFPVFGFFAYLLFGQNFRRKRMFQKKALLDEQAFLQYKGHED
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHCCCCCC
YEERILRNHKHQELLFRLADRLGALNISFQTETRTLTNGDETFQAILDGLKRAKHHIHME
HHHHHHHCCCHHHHHHHHHHHHCCEEEEEEECCCEECCCHHHHHHHHHHHHHHHHEEEEE
YYIVRDDKLGTEIKDILIQKSKEGVVVRFLYDAVGSFKLSKSYIEELNDAGVEVIPFFPV
EEEEECCCCCHHHHHHHHHCCCCCEEEEEEHHHHCCCHHHHHHHHHHHCCCCEEEEECCE
RFPILNDKINYRNHRKIVIIDGNEGFVGGLNIGDEYLGKDKYFGFWRDTHLYLRGEAVQS
EEEEECCCCCCCCCCEEEEEECCCCEEECCCCCHHHCCCCCCEEEEECEEEEEECCHHHH
LQLIFLQDWFYMTGEAVLAPEYLQAKAVEGEHWGGVQLVAGGPDNKWETIKHLYFAMIAS
HHHHHHHHHHHHCCCEEECCHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHH
ARKSIWIATPYFIPDDDILSALKVAALAGIDVRLLMPSKPDKRTVFYASRSYFPELLDAG
CCCCEEEECCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEECHHCCHHHHHCC
VKIYEYEKGFLHSKVVIVDSDLASIGTANMDMRSFHLNFEVNAFLYDTDSIRKLVQDFKD
CEEEEECCCCCEEEEEEEECCHHHCCCCCCCEEEEEEEEEEEEEEECCHHHHHHHHHHHH
DLEESSEIHVDRFHKRRLHRRIVESTYRLLSPLL
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKNTLKLIFFVLLLFALFVSLRMFIDVAFYSDVIGIKDVSILGIISILFTVSAFLIGCVI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
FLENRHPSKTLTWLIVLGIFPVFGFFAYLLFGQNFRRKRMFQKKALLDEQAFLQYKGHED
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHCCCCCC
YEERILRNHKHQELLFRLADRLGALNISFQTETRTLTNGDETFQAILDGLKRAKHHIHME
HHHHHHHCCCHHHHHHHHHHHHCCEEEEEEECCCEECCCHHHHHHHHHHHHHHHHEEEEE
YYIVRDDKLGTEIKDILIQKSKEGVVVRFLYDAVGSFKLSKSYIEELNDAGVEVIPFFPV
EEEEECCCCCHHHHHHHHHCCCCCEEEEEEHHHHCCCHHHHHHHHHHHCCCCEEEEECCE
RFPILNDKINYRNHRKIVIIDGNEGFVGGLNIGDEYLGKDKYFGFWRDTHLYLRGEAVQS
EEEEECCCCCCCCCCEEEEEECCCCEEECCCCCHHHCCCCCCEEEEECEEEEEECCHHHH
LQLIFLQDWFYMTGEAVLAPEYLQAKAVEGEHWGGVQLVAGGPDNKWETIKHLYFAMIAS
HHHHHHHHHHHHCCCEEECCHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHH
ARKSIWIATPYFIPDDDILSALKVAALAGIDVRLLMPSKPDKRTVFYASRSYFPELLDAG
CCCCEEEECCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEECHHCCHHHHHCC
VKIYEYEKGFLHSKVVIVDSDLASIGTANMDMRSFHLNFEVNAFLYDTDSIRKLVQDFKD
CEEEEECCCCCEEEEEEEECCHHHCCCCCCCEEEEEEEEEEEEEEECCHHHHHHHHHHHH
DLEESSEIHVDRFHKRRLHRRIVESTYRLLSPLL
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]