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The map label for this gene is prpA [H]

Identifier: 52144128

GI number: 52144128

Start: 1209068

End: 1209808

Strand: Reverse

Name: prpA [H]

Synonym: BCZK1099

Alternate gene names: 52144128

Gene position: 1209808-1209068 (Counterclockwise)

Preceding gene: 52144126

Following gene: 52144129

Centisome position: 22.82

GC content: 41.03

Gene sequence:

>741_bases
ATGAAATATGACATTATAGGGGATATACATGGATGCTTACAAGAGTTTCAAAATTTAACAGAGAAGCTAGGGTATAACTG
GAGTAGCGGTCTACCGGTTCACCCTGATCAACGCAAACTTGCATTTGTTGGTGATATTACAGATCGCGGCCCTCATTCAT
TACGTATGATTGAAATTGTATGGGAACTTGTCATACATAAAAAAGTAGCTTACTACGCTCCGGGAAATCACTGTAATAAA
CTATACCGATTTTTCCTTGGGCGTAATGTAACAATCGCTCATGGACTCGAAACAACTGTTGCTGAATATGAAGCACTGCC
TTCTCATAAACAAAACATGATAAAAGAAAAATTCATCACACTTTACGAGCAATCCCCTTTATATCACGTACTCGATGAGA
AAAGGTTACTCGTGTGTCACGCCGGCATTCGGCAAGATTACATAGGAAGACAAGATAAAAAAGTACAAACCTTTGTATTA
TATGGTGATATCACTGGAGAAAAACATGCTGACGGCTCACCTGTCCGCCGCGACTGGGCGAAGGAGTATAAAGGAACAAC
TTGGATTGTATATGGACATACACCCGTGAAAGAACCTCGCTTTGTAAATCATACAGTCAATATTGATACTGGTGCTGTAT
TTGGTGGTAAACTAACCGGGCTACGTTATCCCGAAATGGAGACCGTTTCCGTCCCTTCCTCCCTGCCTTTTGTCCCTGAG
AAATTTCGCCCAATTTCATAA

Upstream 100 bases:

>100_bases
GAACGAAAATCTTTATGTTTAACTGAAGCACAAAAACCGTATATTTTTATCTCTATCCTTTTTTAGATATACTAATGAAA
AATATGATGGAGGTTTAAAT

Downstream 100 bases:

>100_bases
CCCATATGTAATACTTACGAAAGATTACCGTATCATTTAGGCGCCTCTCATATAAAAACTGGGTAAAGTCATTAAAAAAA
GATGCAATAAAGTGCGTCTT

Product: bis(5'-nucleosyl)-tetraphosphatase PrpE

Products: NA

Alternate protein names: Ap4A hydrolase; Diadenosine 5',5'''-P1,P4-tetraphosphate asymmetrical hydrolase; Diadenosine tetraphosphatase [H]

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MKYDIIGDIHGCLQEFQNLTEKLGYNWSSGLPVHPDQRKLAFVGDITDRGPHSLRMIEIVWELVIHKKVAYYAPGNHCNK
LYRFFLGRNVTIAHGLETTVAEYEALPSHKQNMIKEKFITLYEQSPLYHVLDEKRLLVCHAGIRQDYIGRQDKKVQTFVL
YGDITGEKHADGSPVRRDWAKEYKGTTWIVYGHTPVKEPRFVNHTVNIDTGAVFGGKLTGLRYPEMETVSVPSSLPFVPE
KFRPIS

Sequences:

>Translated_246_residues
MKYDIIGDIHGCLQEFQNLTEKLGYNWSSGLPVHPDQRKLAFVGDITDRGPHSLRMIEIVWELVIHKKVAYYAPGNHCNK
LYRFFLGRNVTIAHGLETTVAEYEALPSHKQNMIKEKFITLYEQSPLYHVLDEKRLLVCHAGIRQDYIGRQDKKVQTFVL
YGDITGEKHADGSPVRRDWAKEYKGTTWIVYGHTPVKEPRFVNHTVNIDTGAVFGGKLTGLRYPEMETVSVPSSLPFVPE
KFRPIS
>Mature_246_residues
MKYDIIGDIHGCLQEFQNLTEKLGYNWSSGLPVHPDQRKLAFVGDITDRGPHSLRMIEIVWELVIHKKVAYYAPGNHCNK
LYRFFLGRNVTIAHGLETTVAEYEALPSHKQNMIKEKFITLYEQSPLYHVLDEKRLLVCHAGIRQDYIGRQDKKVQTFVL
YGDITGEKHADGSPVRRDWAKEYKGTTWIVYGHTPVKEPRFVNHTVNIDTGAVFGGKLTGLRYPEMETVSVPSSLPFVPE
KFRPIS

Specific function: Asymmetrically hydrolyzes Ap4p to yield AMP and ATP [H]

COG id: COG0639

COG function: function code T; Diadenosine tetraphosphatase and related serine/threonine protein phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prpE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: =3.6.1.17 [H]

Molecular weight: Translated: 28214; Mature: 28214

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYDIIGDIHGCLQEFQNLTEKLGYNWSSGLPVHPDQRKLAFVGDITDRGPHSLRMIEIV
CCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHH
WELVIHKKVAYYAPGNHCNKLYRFFLGRNVTIAHGLETTVAEYEALPSHKQNMIKEKFIT
HHHHHHHHHEEECCCHHHHHHHHHHHCCCEEEECCCHHHHHHHHHCCHHHHHHHHHHHHH
LYEQSPLYHVLDEKRLLVCHAGIRQDYIGRQDKKVQTFVLYGDITGEKHADGSPVRRDWA
HHCCCCCHHHHCCCEEEEEECCCHHHHCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHH
KEYKGTTWIVYGHTPVKEPRFVNHTVNIDTGAVFGGKLTGLRYPEMETVSVPSSLPFVPE
HHHCCCEEEEECCCCCCCCCEEEEEEECCCCEEECCEECCCCCCCCCEEECCCCCCCCCC
KFRPIS
CCCCCC
>Mature Secondary Structure
MKYDIIGDIHGCLQEFQNLTEKLGYNWSSGLPVHPDQRKLAFVGDITDRGPHSLRMIEIV
CCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHH
WELVIHKKVAYYAPGNHCNKLYRFFLGRNVTIAHGLETTVAEYEALPSHKQNMIKEKFIT
HHHHHHHHHEEECCCHHHHHHHHHHHCCCEEEECCCHHHHHHHHHCCHHHHHHHHHHHHH
LYEQSPLYHVLDEKRLLVCHAGIRQDYIGRQDKKVQTFVLYGDITGEKHADGSPVRRDWA
HHCCCCCHHHHCCCEEEEEECCCHHHHCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHH
KEYKGTTWIVYGHTPVKEPRFVNHTVNIDTGAVFGGKLTGLRYPEMETVSVPSSLPFVPE
HHHCCCEEEEECCCCCCCCCEEEEEEECCCCEEECCEECCCCCCCCCEEECCCCCCCCCC
KFRPIS
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA