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The map label for this gene is rfbB [H]

Identifier: 52144116

GI number: 52144116

Start: 1219633

End: 1220601

Strand: Direct

Name: rfbB [H]

Synonym: BCZK1111

Alternate gene names: 52144116

Gene position: 1219633-1220601 (Clockwise)

Preceding gene: 52144117

Following gene: 52144115

Centisome position: 23.01

GC content: 35.19

Gene sequence:

>969_bases
ATGAATATATTAGTAACGGGTGGGGCCGGATTTATTGGAAGTAATTTTGTACATTATATGTTACAAAGCTATGAAACATA
TAAAATTATTAATTTTGATGCATTAACATATAGTGGAAACTTAAAAAATGTAAAGTCTATTCAAGATCATCCGAATTACT
ATTTTGTAAAAGGGGAAATTCAAAATGGGGAGCTGCTGGAGCATGTTATTAAGGAACGTGATGTGCAAGTAATCGTCAAT
TTCGCAGCTGAATCACATGTAGATCGTAGTATTGAAAATCCAATCCCTTTTTATGATACAAATGTAATTGGGACGGTCAC
CTTACTAGAATTAGTAAAAAAATATCCGCATATTAAACTTGTGCAAGTATCAACGGATGAGGTGTATGGCTCGTTAGGAA
AAACAGGGCGATTTACCGAGGAAACACCGTTAGCTCCAAATAGTCCATATTCTTCAAGCAAAGCGAGCGCCGATATGATA
GCTTTAGCTTATTATAAAACATATCAATTACCAGTTATAGTAACGCGTTGTTCCAATAACTATGGGCCGTATCAATATCC
TGAAAAATTAATCCCGTTAATGGTTACGAATGCGCTCGAAGGAAAAAAATTACCGTTATATGGCGATGGATTAAATGTAA
GAGATTGGTTACATGTAACAGATCATTGTAGTGCGATTGACGTTGTTCTGCATAAGGGCCGTATAGGAGAAGTATATAAT
ATAGGTGGAAATAATGAAAAAACAAATATAGATGTTGTGGAACAAATTATTACCCTCTTAGGAAAAACGGAAAAAGATAT
TGAATATGTGACAGATCGTTTAGGACACGACCGCCGTTATGCGATTGATGCAGAGAAAATGAAGAATGAATTTGATTGGG
AACCGAAGTATACGTTTGAGCAAGGATTGCAAGAAACAGTGCAATGGTATGAGAAGAATGAGGAATGGTGGAAACCATTA
AAAAAGTAA

Upstream 100 bases:

>100_bases
AATTCCATGGCCGGTAACAAGTCCTATTTTGTCTGATAAAGATCGAATATTACCATTACTTCAGGAATGTAAAGATAGCT
TTTGAGTAGGAGAGTTGTTT

Downstream 100 bases:

>100_bases
GGGGCATATAAATGAAAGAACGGATTATCATAACAGGAGCAAATGGTCAATTAGGAAAGCAGCTACAAGAAGAGTTAAAT
CCTGAAGAATATGACATATA

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MNILVTGGAGFIGSNFVHYMLQSYETYKIINFDALTYSGNLKNVKSIQDHPNYYFVKGEIQNGELLEHVIKERDVQVIVN
FAAESHVDRSIENPIPFYDTNVIGTVTLLELVKKYPHIKLVQVSTDEVYGSLGKTGRFTEETPLAPNSPYSSSKASADMI
ALAYYKTYQLPVIVTRCSNNYGPYQYPEKLIPLMVTNALEGKKLPLYGDGLNVRDWLHVTDHCSAIDVVLHKGRIGEVYN
IGGNNEKTNIDVVEQIITLLGKTEKDIEYVTDRLGHDRRYAIDAEKMKNEFDWEPKYTFEQGLQETVQWYEKNEEWWKPL
KK

Sequences:

>Translated_322_residues
MNILVTGGAGFIGSNFVHYMLQSYETYKIINFDALTYSGNLKNVKSIQDHPNYYFVKGEIQNGELLEHVIKERDVQVIVN
FAAESHVDRSIENPIPFYDTNVIGTVTLLELVKKYPHIKLVQVSTDEVYGSLGKTGRFTEETPLAPNSPYSSSKASADMI
ALAYYKTYQLPVIVTRCSNNYGPYQYPEKLIPLMVTNALEGKKLPLYGDGLNVRDWLHVTDHCSAIDVVLHKGRIGEVYN
IGGNNEKTNIDVVEQIITLLGKTEKDIEYVTDRLGHDRRYAIDAEKMKNEFDWEPKYTFEQGLQETVQWYEKNEEWWKPL
KK
>Mature_322_residues
MNILVTGGAGFIGSNFVHYMLQSYETYKIINFDALTYSGNLKNVKSIQDHPNYYFVKGEIQNGELLEHVIKERDVQVIVN
FAAESHVDRSIENPIPFYDTNVIGTVTLLELVKKYPHIKLVQVSTDEVYGSLGKTGRFTEETPLAPNSPYSSSKASADMI
ALAYYKTYQLPVIVTRCSNNYGPYQYPEKLIPLMVTNALEGKKLPLYGDGLNVRDWLHVTDHCSAIDVVLHKGRIGEVYN
IGGNNEKTNIDVVEQIITLLGKTEKDIEYVTDRLGHDRRYAIDAEKMKNEFDWEPKYTFEQGLQETVQWYEKNEEWWKPL
KK

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=327, Percent_Identity=38.2262996941896, Blast_Score=245, Evalue=4e-65,
Organism=Homo sapiens, GI42516563, Length=321, Percent_Identity=27.7258566978193, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI4504031, Length=336, Percent_Identity=25.5952380952381, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI56237023, Length=342, Percent_Identity=25.7309941520468, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI56118217, Length=342, Percent_Identity=25.7309941520468, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI189083684, Length=342, Percent_Identity=25.7309941520468, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI48994969, Length=340, Percent_Identity=47.6470588235294, Blast_Score=323, Evalue=9e-90,
Organism=Escherichia coli, GI1788353, Length=341, Percent_Identity=46.6275659824047, Blast_Score=313, Evalue=1e-86,
Organism=Escherichia coli, GI1786974, Length=341, Percent_Identity=27.2727272727273, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1788366, Length=231, Percent_Identity=27.2727272727273, Blast_Score=73, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17568069, Length=322, Percent_Identity=38.8198757763975, Blast_Score=228, Evalue=4e-60,
Organism=Caenorhabditis elegans, GI115532424, Length=318, Percent_Identity=33.3333333333333, Blast_Score=184, Evalue=5e-47,
Organism=Caenorhabditis elegans, GI17539532, Length=329, Percent_Identity=25.531914893617, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI71982038, Length=355, Percent_Identity=27.0422535211268, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71982035, Length=353, Percent_Identity=27.1954674220963, Blast_Score=86, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6319493, Length=347, Percent_Identity=26.8011527377522, Blast_Score=96, Evalue=6e-21,
Organism=Drosophila melanogaster, GI21356223, Length=326, Percent_Identity=28.2208588957055, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI19923002, Length=344, Percent_Identity=28.4883720930233, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24158427, Length=331, Percent_Identity=24.4712990936556, Blast_Score=65, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 36891; Mature: 36891

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILVTGGAGFIGSNFVHYMLQSYETYKIINFDALTYSGNLKNVKSIQDHPNYYFVKGEI
CEEEEECCCCCHHHHHHHHHHHCCCEEEEEEEEEEEECCCCCCHHHHHCCCCEEEEEEEC
QNGELLEHVIKERDVQVIVNFAAESHVDRSIENPIPFYDTNVIGTVTLLELVKKYPHIKL
CCCHHHHHHHHCCCCEEEEEHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCEEE
VQVSTDEVYGSLGKTGRFTEETPLAPNSPYSSSKASADMIALAYYKTYQLPVIVTRCSNN
EEEEHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHEEEEEEHHHCCCEEEEEEECCC
YGPYQYPEKLIPLMVTNALEGKKLPLYGDGLNVRDWLHVTDHCSAIDVVLHKGRIGEVYN
CCCCCCHHHHHHHHHHHCCCCCCCCEECCCCCHHHHEEHHHCCCHHHHHHCCCCCCCEEE
IGGNNEKTNIDVVEQIITLLGKTEKDIEYVTDRLGHDRRYAIDAEKMKNEFDWEPKYTFE
CCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEECHHHHHHCCCCCCCHHHH
QGLQETVQWYEKNEEWWKPLKK
HHHHHHHHHHHCCHHHHHHCCC
>Mature Secondary Structure
MNILVTGGAGFIGSNFVHYMLQSYETYKIINFDALTYSGNLKNVKSIQDHPNYYFVKGEI
CEEEEECCCCCHHHHHHHHHHHCCCEEEEEEEEEEEECCCCCCHHHHHCCCCEEEEEEEC
QNGELLEHVIKERDVQVIVNFAAESHVDRSIENPIPFYDTNVIGTVTLLELVKKYPHIKL
CCCHHHHHHHHCCCCEEEEEHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCEEE
VQVSTDEVYGSLGKTGRFTEETPLAPNSPYSSSKASADMIALAYYKTYQLPVIVTRCSNN
EEEEHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHEEEEEEHHHCCCEEEEEEECCC
YGPYQYPEKLIPLMVTNALEGKKLPLYGDGLNVRDWLHVTDHCSAIDVVLHKGRIGEVYN
CCCCCCHHHHHHHHHHHCCCCCCCCEECCCCCHHHHEEHHHCCCHHHHHHCCCCCCCEEE
IGGNNEKTNIDVVEQIITLLGKTEKDIEYVTDRLGHDRRYAIDAEKMKNEFDWEPKYTFE
CCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEECHHHHHHCCCCCCCHHHH
QGLQETVQWYEKNEEWWKPLKK
HHHHHHHHHHHCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253667 [H]