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The map label for this gene is rbfD [H]

Identifier: 52144115

GI number: 52144115

Start: 1220613

End: 1221467

Strand: Direct

Name: rbfD [H]

Synonym: BCZK1112

Alternate gene names: 52144115

Gene position: 1220613-1221467 (Clockwise)

Preceding gene: 52144116

Following gene: 52144114

Centisome position: 23.03

GC content: 36.73

Gene sequence:

>855_bases
ATGAAAGAACGGATTATCATAACAGGAGCAAATGGTCAATTAGGAAAGCAGCTACAAGAAGAGTTAAATCCTGAAGAATA
TGACATATATCCATTTGATAAAAAGTTGTTAGATATAACAAATATATCCCGAGTGCAGCAAGTGGTACAAGAAATAAGGC
CACATATCATTATTCATTGTGCAGCGTATACGAAGGTTGATCAAGCTGAGAAAGAGCGAGATCTTGCTTATGTAATAAAT
GCAATAGGGGCTCGAAATGTAGCGGTTGCTTCACAATTAGTTGGGGCGAAGTTAGTTTATATTAGTACGGATTATGTATT
TCAAGGCGACAGACCGGAGGGGTACGATGAATTTCATAATCCGGCGCCGATCAATATATACGGGGCTTCTAAATATGCGG
GAGAGCAGTTCGTCAAAGAGTTACATAATAAATACTTTATCGTTCGTACATCGTGGTTATATGGTAAGTATGGAAATAAT
TTTGTGAAAACGATGATACGATTAGGGAAAGAAAGAGAAGAAATATCTGTTGTAGCGGATCAAATCGGTTCTCCTACGTA
TGTGGCGGATTTAAATGTGATGATTAATAAGCTCATTCATACTTCTTTATACGGTACGTATCACGTATCCAATACAGGAT
CATGTTCTTGGTTTGGATTTGCAAAGAAAATATTTTCGTATGCAAATATGAAGGTGAATGTGTTACCTGTTTCAACCGAA
GAATTTGGGGCTGTAGCAGCAAGGCCGAAATATTCTATTTTCCAACATAACATGCTACGATTAAATGGTTTTTTGCAAAT
GCCTTCTTGGGAAGAAGGATTAGAGCGTTTTTTTATAGAAACCAAAAGTCATTAA

Upstream 100 bases:

>100_bases
AACCGAAGTATACGTTTGAGCAAGGATTGCAAGAAACAGTGCAATGGTATGAGAAGAATGAGGAATGGTGGAAACCATTA
AAAAAGTAAGGGGCATATAA

Downstream 100 bases:

>100_bases
CAATTTTAAGTTAATGACTTTTTTGTTTGCCTTTAAGAGGTTTTATGTTACTATAATTATAGTATCAGGTACTAATAACA
AGTATAAGTATTTCTGGGAG

Product: dTDP-4-dehydrorhamnose reductase

Products: dTDP-4-dehydro-6-deoxy-L-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MKERIIITGANGQLGKQLQEELNPEEYDIYPFDKKLLDITNISRVQQVVQEIRPHIIIHCAAYTKVDQAEKERDLAYVIN
AIGARNVAVASQLVGAKLVYISTDYVFQGDRPEGYDEFHNPAPINIYGASKYAGEQFVKELHNKYFIVRTSWLYGKYGNN
FVKTMIRLGKEREEISVVADQIGSPTYVADLNVMINKLIHTSLYGTYHVSNTGSCSWFGFAKKIFSYANMKVNVLPVSTE
EFGAVAARPKYSIFQHNMLRLNGFLQMPSWEEGLERFFIETKSH

Sequences:

>Translated_284_residues
MKERIIITGANGQLGKQLQEELNPEEYDIYPFDKKLLDITNISRVQQVVQEIRPHIIIHCAAYTKVDQAEKERDLAYVIN
AIGARNVAVASQLVGAKLVYISTDYVFQGDRPEGYDEFHNPAPINIYGASKYAGEQFVKELHNKYFIVRTSWLYGKYGNN
FVKTMIRLGKEREEISVVADQIGSPTYVADLNVMINKLIHTSLYGTYHVSNTGSCSWFGFAKKIFSYANMKVNVLPVSTE
EFGAVAARPKYSIFQHNMLRLNGFLQMPSWEEGLERFFIETKSH
>Mature_284_residues
MKERIIITGANGQLGKQLQEELNPEEYDIYPFDKKLLDITNISRVQQVVQEIRPHIIIHCAAYTKVDQAEKERDLAYVIN
AIGARNVAVASQLVGAKLVYISTDYVFQGDRPEGYDEFHNPAPINIYGASKYAGEQFVKELHNKYFIVRTSWLYGKYGNN
FVKTMIRLGKEREEISVVADQIGSPTYVADLNVMINKLIHTSLYGTYHVSNTGSCSWFGFAKKIFSYANMKVNVLPVSTE
EFGAVAARPKYSIFQHNMLRLNGFLQMPSWEEGLERFFIETKSH

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI11034825, Length=301, Percent_Identity=27.5747508305648, Blast_Score=104, Evalue=8e-23,
Organism=Homo sapiens, GI33519455, Length=301, Percent_Identity=27.5747508305648, Blast_Score=104, Evalue=1e-22,
Organism=Escherichia coli, GI1788352, Length=296, Percent_Identity=37.1621621621622, Blast_Score=185, Evalue=3e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133

Molecular weight: Translated: 32409; Mature: 32409

Theoretical pI: Translated: 7.71; Mature: 7.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKERIIITGANGQLGKQLQEELNPEEYDIYPFDKKLLDITNISRVQQVVQEIRPHIIIHC
CCCEEEEECCCCHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCEEEEEE
AAYTKVDQAEKERDLAYVINAIGARNVAVASQLVGAKLVYISTDYVFQGDRPEGYDEFHN
EHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCEEEEEEEECEEEECCCCCCHHHCCC
PAPINIYGASKYAGEQFVKELHNKYFIVRTSWLYGKYGNNFVKTMIRLGKEREEISVVAD
CCCEEEEECCHHHHHHHHHHHCCCEEEEEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHH
QIGSPTYVADLNVMINKLIHTSLYGTYHVSNTGSCSWFGFAKKIFSYANMKVNVLPVSTE
HCCCCEEEEHHHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEEECHH
EFGAVAARPKYSIFQHNMLRLNGFLQMPSWEEGLERFFIETKSH
HHCCCCCCCCHHHHHCCCEEEECEEECCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKERIIITGANGQLGKQLQEELNPEEYDIYPFDKKLLDITNISRVQQVVQEIRPHIIIHC
CCCEEEEECCCCHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCEEEEEE
AAYTKVDQAEKERDLAYVINAIGARNVAVASQLVGAKLVYISTDYVFQGDRPEGYDEFHN
EHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCEEEEEEEECEEEECCCCCCHHHCCC
PAPINIYGASKYAGEQFVKELHNKYFIVRTSWLYGKYGNNFVKTMIRLGKEREEISVVAD
CCCEEEEECCHHHHHHHHHHHCCCEEEEEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHH
QIGSPTYVADLNVMINKLIHTSLYGTYHVSNTGSCSWFGFAKKIFSYANMKVNVLPVSTE
HCCCCEEEEHHHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEEECHH
EFGAVAARPKYSIFQHNMLRLNGFLQMPSWEEGLERFFIETKSH
HHCCCCCCCCHHHHHCCCEEEECEEECCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-6-deoxy-L-mannose; NADP+

Specific reaction: dTDP-6-deoxy-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-L-mannose + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]