Click here to switch to the map view.

The map label for this gene is dacF [H]

Identifier: 52144058

GI number: 52144058

Start: 1270198

End: 1271484

Strand: Direct

Name: dacF [H]

Synonym: BCZK1171

Alternate gene names: 52144058

Gene position: 1270198-1271484 (Clockwise)

Preceding gene: 52144059

Following gene: 52144056

Centisome position: 23.96

GC content: 36.05

Gene sequence:

>1287_bases
ATGGTAAATTTTAAGAAAATTTTGGCTTTTATTACCGTTATTTGTTTATTTTTTTTGACACCTATGACGTTACGTGCTGA
GACGAATATTGGTGTGAACCCAGAACAAGTGGCACCACCACCTAAGGAAGGGCCAAATGTTTTTAGTCAGTTTGCAACTA
CAATTGATGCGAAAACTGGAGATATTTTGTACGATAAAAACGCACATCACCGTGCATTTCCGGCTAGTATGACGAAAGTG
TTAACGGCGATTTTACTTATGGAACATACGAAGCCAGAGGATCAATTTACGTTTTCACAATTAGCATTAGATCAAGAGAA
GAGTAATTATCAAATTGAGTTTCAACCTGGTGAGACGATTAATAGAAATACTGCACTTATGATTTTAATGGTGCTGAGTG
CAAATGATGTGTCGTATGCGATTGCGGAGCGTATTGGCGGAAGTGTTGAGAATTTTGCTAATATGATGAATGAGAGAGCG
AAGCAATTAGGCGCTACCGACAGTCATTTTGTAACACCAAATGGATTGCATGATCCGAACCATTATACTACGCCATATGA
TATGGCTATGATTACGAGAGGTGTGCAAAAGTATCCTGAAATCTTACAAGCGATGAATACGAAAAGAACGACAGTTACGA
CATCTAGGCAGACGGTGTCTATTTTTAATAAATCTACTTATTTTGAAAATCCATATAGTATTGGTGGTAAGACAGGTTTT
ACAAATGAAGCGCGCAATACACTCGTTTTATTAAATGAGAAGGATGGAAATCGTATTATAAATGTAGTAATGGCTTCTCA
AAGACCTGAAATTTATGAAGATTTAAAGCAGATGGCGGACTATTCTTTCGGTCAATTTGTGAAGCAAACTGTACTGGATA
AACATAGTTGGCATCAAAAGACAACGTATTTAAATAAAGATATTGATAGTGAGCTTGAAAAAAGTGCAGAACTAATGCTT
AAGAAAGATGAAGGGAAAAATGTGAAGACGACGTTCCGGGCGGCTACATTAGATAAAGATTCTTTGTATCATAAAGGAAT
TCATCGTGGTGAAGTAGTTGGTGCAGTTGATATTACGAAAAATAATCAAACAATTGCGACGGTAAATGTTCTTTCTAAAG
AAGACGTTACTTTTGCGATGCCTAAAAAAGATACGATCGAGCCTGAAGTAAATGAATCAAACGTGAAAATAATAAGCATT
GGAATAGGTGCTGCCATATTATTTGGAGCCATTTTATTTGTAGTGTTACGCAGAAATACAAAAGGAATGAAGTCAGAAGA
AAAATAA

Upstream 100 bases:

>100_bases
GTAAAAAACAGTGAATGTTCTTCCAATTTAATACAAATTACATATAGATTGGTAGATGCAGTTTTTAAGCTGTGAGAGTA
TAGTGAAAAGGGAGAGTCAT

Downstream 100 bases:

>100_bases
ATTACTAGTAAAAAAGACTGTGAGGGAATATTGACACAGTCTTTTTTACGCTTTAATTAACTAATAATTGTAAATCTTCT
TATTGCAACTATTTGGAGTT

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5*; PBP-5* [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MVNFKKILAFITVICLFFLTPMTLRAETNIGVNPEQVAPPPKEGPNVFSQFATTIDAKTGDILYDKNAHHRAFPASMTKV
LTAILLMEHTKPEDQFTFSQLALDQEKSNYQIEFQPGETINRNTALMILMVLSANDVSYAIAERIGGSVENFANMMNERA
KQLGATDSHFVTPNGLHDPNHYTTPYDMAMITRGVQKYPEILQAMNTKRTTVTTSRQTVSIFNKSTYFENPYSIGGKTGF
TNEARNTLVLLNEKDGNRIINVVMASQRPEIYEDLKQMADYSFGQFVKQTVLDKHSWHQKTTYLNKDIDSELEKSAELML
KKDEGKNVKTTFRAATLDKDSLYHKGIHRGEVVGAVDITKNNQTIATVNVLSKEDVTFAMPKKDTIEPEVNESNVKIISI
GIGAAILFGAILFVVLRRNTKGMKSEEK

Sequences:

>Translated_428_residues
MVNFKKILAFITVICLFFLTPMTLRAETNIGVNPEQVAPPPKEGPNVFSQFATTIDAKTGDILYDKNAHHRAFPASMTKV
LTAILLMEHTKPEDQFTFSQLALDQEKSNYQIEFQPGETINRNTALMILMVLSANDVSYAIAERIGGSVENFANMMNERA
KQLGATDSHFVTPNGLHDPNHYTTPYDMAMITRGVQKYPEILQAMNTKRTTVTTSRQTVSIFNKSTYFENPYSIGGKTGF
TNEARNTLVLLNEKDGNRIINVVMASQRPEIYEDLKQMADYSFGQFVKQTVLDKHSWHQKTTYLNKDIDSELEKSAELML
KKDEGKNVKTTFRAATLDKDSLYHKGIHRGEVVGAVDITKNNQTIATVNVLSKEDVTFAMPKKDTIEPEVNESNVKIISI
GIGAAILFGAILFVVLRRNTKGMKSEEK
>Mature_428_residues
MVNFKKILAFITVICLFFLTPMTLRAETNIGVNPEQVAPPPKEGPNVFSQFATTIDAKTGDILYDKNAHHRAFPASMTKV
LTAILLMEHTKPEDQFTFSQLALDQEKSNYQIEFQPGETINRNTALMILMVLSANDVSYAIAERIGGSVENFANMMNERA
KQLGATDSHFVTPNGLHDPNHYTTPYDMAMITRGVQKYPEILQAMNTKRTTVTTSRQTVSIFNKSTYFENPYSIGGKTGF
TNEARNTLVLLNEKDGNRIINVVMASQRPEIYEDLKQMADYSFGQFVKQTVLDKHSWHQKTTYLNKDIDSELEKSAELML
KKDEGKNVKTTFRAATLDKDSLYHKGIHRGEVVGAVDITKNNQTIATVNVLSKEDVTFAMPKKDTIEPEVNESNVKIISI
GIGAAILFGAILFVVLRRNTKGMKSEEK

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. Required specifically for the synthesis of the spore form of peptidoglycan (cortex) [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Forespore outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=375, Percent_Identity=24.5333333333333, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI87082022, Length=384, Percent_Identity=24.4791666666667, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1786851, Length=375, Percent_Identity=24, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI87082054, Length=224, Percent_Identity=26.3392857142857, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 48009; Mature: 48009

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNFKKILAFITVICLFFLTPMTLRAETNIGVNPEQVAPPPKEGPNVFSQFATTIDAKTG
CCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHCCCCCCCCHHHHHHHHHHHCCCCC
DILYDKNAHHRAFPASMTKVLTAILLMEHTKPEDQFTFSQLALDQEKSNYQIEFQPGETI
CEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCC
NRNTALMILMVLSANDVSYAIAERIGGSVENFANMMNERAKQLGATDSHFVTPNGLHDPN
CCCCEEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCC
HYTTPYDMAMITRGVQKYPEILQAMNTKRTTVTTSRQTVSIFNKSTYFENPYSIGGKTGF
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEHCCCCCCCCCCCCCCCCCC
TNEARNTLVLLNEKDGNRIINVVMASQRPEIYEDLKQMADYSFGQFVKQTVLDKHSWHQK
CCCCCCEEEEEECCCCCEEEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHH
TTYLNKDIDSELEKSAELMLKKDEGKNVKTTFRAATLDKDSLYHKGIHRGEVVGAVDITK
HHHHCCCHHHHHHHHHHHEEECCCCCCCEEEEEEECCCHHHHHHCCCCCCCEEEEEEEEC
NNQTIATVNVLSKEDVTFAMPKKDTIEPEVNESNVKIISIGIGAAILFGAILFVVLRRNT
CCCEEEEEEEECCCCEEEECCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHCCC
KGMKSEEK
CCCCCCCC
>Mature Secondary Structure
MVNFKKILAFITVICLFFLTPMTLRAETNIGVNPEQVAPPPKEGPNVFSQFATTIDAKTG
CCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHCCCCCCCCHHHHHHHHHHHCCCCC
DILYDKNAHHRAFPASMTKVLTAILLMEHTKPEDQFTFSQLALDQEKSNYQIEFQPGETI
CEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCC
NRNTALMILMVLSANDVSYAIAERIGGSVENFANMMNERAKQLGATDSHFVTPNGLHDPN
CCCCEEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCC
HYTTPYDMAMITRGVQKYPEILQAMNTKRTTVTTSRQTVSIFNKSTYFENPYSIGGKTGF
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEHCCCCCCCCCCCCCCCCCC
TNEARNTLVLLNEKDGNRIINVVMASQRPEIYEDLKQMADYSFGQFVKQTVLDKHSWHQK
CCCCCCEEEEEECCCCCEEEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHH
TTYLNKDIDSELEKSAELMLKKDEGKNVKTTFRAATLDKDSLYHKGIHRGEVVGAVDITK
HHHHCCCHHHHHHHHHHHEEECCCCCCCEEEEEEECCCHHHHHHCCCCCCCEEEEEEEEC
NNQTIATVNVLSKEDVTFAMPKKDTIEPEVNESNVKIISIGIGAAILFGAILFVVLRRNT
CCCEEEEEEEECCCCEEEECCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHCCC
KGMKSEEK
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1548223; 7934829; 9384377 [H]