Click here to switch to the map view.

The map label for this gene is ilvB [H]

Identifier: 52143947

GI number: 52143947

Start: 1375025

End: 1376725

Strand: Direct

Name: ilvB [H]

Synonym: BCZK1282

Alternate gene names: 52143947

Gene position: 1375025-1376725 (Clockwise)

Preceding gene: 52143949

Following gene: 52143946

Centisome position: 25.94

GC content: 40.15

Gene sequence:

>1701_bases
ATGTCTTCAAAAACGGAAGAGAAACTGGCAACCGGTGCACAGCTATTGTTAGAAGCGCTAGAAAAGGAAGGAGTAGAAGT
GATTTTTGGTTATCCTGGTGGTGCAGTTTTACCTCTTTATGATGCGCTCTATGACTGTGAAATTCCGCATATTTTAACAA
GGCATGAACAAGGAGCGATTCACGCCGCGGAAGGGTATGCCAGAATTACTGGCAATCCAGGGGTTGTAATTGCAACGAGT
GGACCAGGTGCTACGAATGTAATTACTGGTCTAGCAGATGCGATGATTGATTCATTGCCACTTGTTGTATTTACAGGGCA
AGTAGCAACAACGTTAATTGGAAGCGATGCTTTCCAAGAAGCGGATATTATGGGGCTTACGATGCCAGTGACAAAACATA
ACTATCAAGTGCGAAAGGCTTCAGATTTACCTCGAATTATTAAAGAGGCATTTCATATTGCTAGAACAGGAAGACCAGGC
CCAGTCGTTATTGACCTTCCGAAAGATATGGTAGTAGAGCAAGGCGAACGATGTAGTGACGTGCAAATGGATTTACCAGG
ATACAACCCTAATTATGAACCGAATCTACTGCAAATAAACAAATTATTACAAGCAATTCAGGTTGCAAACAAGCCATTAA
TTTTAGCTGGAGCAGGTGTATTGCATGCGAAAGCTTCAAAGGAATTAACAAGTTTTGCTCGTAAATATGAAATTCCTGTT
GTGCATACATTACTTGGTCTTGGTGGGTTTCCACCAGATGATGAACTATTTCTAGGGATGGGAGGAATGCATGGTTCTTA
CACAGCTAATATGGCGTTATATGAATGCGACTTACTCATTAATATAGGCGCTAGATTCGATGATCGTCTTACTGGAAATT
TAGCTTATTTTGCGAAAGAGGCGACTGTCGCGCATATCGATATTGATCCAGCAGAAATCGGAAAAAATGTACCGACTGAA
ATTCCTATTGTTGCAAGTGCTAAGCAAGCGCTCCAAGTATTACTTCAACCAGAAGGCAGCAAAGAAAATCATCATGAGTG
GATCTCTTTATTAAAGAATAGAAAAGAAAAGTATCCATTCTCTTATAAACGAAATTCTGAAAGTATTAAGCCTCAATATG
CAATTGATATGTTATATGAAATTACGAAGGGAGAAGCGATTGTAACAACAGATGTGGGGCAACATCAAATGTGGGCAGCC
CAATATTATCCGCTGAAAACTCCAGATAAATGGGTAACTTCAGGAGGACTAGGAACGATGGGCTTCGGATTTCCAGCAGC
AATTGGTGCCCAAATTGCAAAGCCTGATGAATTAGTTATTGCAATTGTTGGTGACGCTGGATTTCAAATGACTCTGCAAG
AATTAAGTGTGTTAAAAGAACACTCTTTACCTGTTAAAGTATTTATTTTAAATAATGAAGCTTTAGGAATGGTAAGACAA
TGGCAAGATGAATTTTATAACCAAAGATATTCGCACTCTTTACTATCGTGTCAACCAGATTTCGTTGCTCTTGCGAATGC
GTATGGCATAAAAGGGGTTCGCATAGATGATCCACTTCTTGCAAAGAAACAATTGCAGCATGCAATTCAATTACAAGAGC
CAGTTGTAATTGATTGCCGTGTACTTCAATCAGAAAAGGTAATGCCGATGGTTGCGCCAGGGAAAGGTGTTCACCAAATG
GAGGGAGTGGAAAAAAGGTGA

Upstream 100 bases:

>100_bases
TGGTACCGCGAAATCTTTCGTCCCTTCAGCACATAGCTGGAGTGTGGACGTAGGATTTTTTTTATTGATAAAAGTTTTTA
AAAGGGGGTTTATAAGAACA

Downstream 100 bases:

>100_bases
AGAGAATTGTTACAGCAACAGTTCGAAATCAAAGTGGTGTGTTAAACCGAATTACAGGTGTTATGACACGAAGACATTTT
AATATTGAAAGTATTTCAGT

Product: acetolactate synthase catalytic subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS; Vegetative protein 105; VEG105 [H]

Number of amino acids: Translated: 566; Mature: 565

Protein sequence:

>566_residues
MSSKTEEKLATGAQLLLEALEKEGVEVIFGYPGGAVLPLYDALYDCEIPHILTRHEQGAIHAAEGYARITGNPGVVIATS
GPGATNVITGLADAMIDSLPLVVFTGQVATTLIGSDAFQEADIMGLTMPVTKHNYQVRKASDLPRIIKEAFHIARTGRPG
PVVIDLPKDMVVEQGERCSDVQMDLPGYNPNYEPNLLQINKLLQAIQVANKPLILAGAGVLHAKASKELTSFARKYEIPV
VHTLLGLGGFPPDDELFLGMGGMHGSYTANMALYECDLLINIGARFDDRLTGNLAYFAKEATVAHIDIDPAEIGKNVPTE
IPIVASAKQALQVLLQPEGSKENHHEWISLLKNRKEKYPFSYKRNSESIKPQYAIDMLYEITKGEAIVTTDVGQHQMWAA
QYYPLKTPDKWVTSGGLGTMGFGFPAAIGAQIAKPDELVIAIVGDAGFQMTLQELSVLKEHSLPVKVFILNNEALGMVRQ
WQDEFYNQRYSHSLLSCQPDFVALANAYGIKGVRIDDPLLAKKQLQHAIQLQEPVVIDCRVLQSEKVMPMVAPGKGVHQM
EGVEKR

Sequences:

>Translated_566_residues
MSSKTEEKLATGAQLLLEALEKEGVEVIFGYPGGAVLPLYDALYDCEIPHILTRHEQGAIHAAEGYARITGNPGVVIATS
GPGATNVITGLADAMIDSLPLVVFTGQVATTLIGSDAFQEADIMGLTMPVTKHNYQVRKASDLPRIIKEAFHIARTGRPG
PVVIDLPKDMVVEQGERCSDVQMDLPGYNPNYEPNLLQINKLLQAIQVANKPLILAGAGVLHAKASKELTSFARKYEIPV
VHTLLGLGGFPPDDELFLGMGGMHGSYTANMALYECDLLINIGARFDDRLTGNLAYFAKEATVAHIDIDPAEIGKNVPTE
IPIVASAKQALQVLLQPEGSKENHHEWISLLKNRKEKYPFSYKRNSESIKPQYAIDMLYEITKGEAIVTTDVGQHQMWAA
QYYPLKTPDKWVTSGGLGTMGFGFPAAIGAQIAKPDELVIAIVGDAGFQMTLQELSVLKEHSLPVKVFILNNEALGMVRQ
WQDEFYNQRYSHSLLSCQPDFVALANAYGIKGVRIDDPLLAKKQLQHAIQLQEPVVIDCRVLQSEKVMPMVAPGKGVHQM
EGVEKR
>Mature_565_residues
SSKTEEKLATGAQLLLEALEKEGVEVIFGYPGGAVLPLYDALYDCEIPHILTRHEQGAIHAAEGYARITGNPGVVIATSG
PGATNVITGLADAMIDSLPLVVFTGQVATTLIGSDAFQEADIMGLTMPVTKHNYQVRKASDLPRIIKEAFHIARTGRPGP
VVIDLPKDMVVEQGERCSDVQMDLPGYNPNYEPNLLQINKLLQAIQVANKPLILAGAGVLHAKASKELTSFARKYEIPVV
HTLLGLGGFPPDDELFLGMGGMHGSYTANMALYECDLLINIGARFDDRLTGNLAYFAKEATVAHIDIDPAEIGKNVPTEI
PIVASAKQALQVLLQPEGSKENHHEWISLLKNRKEKYPFSYKRNSESIKPQYAIDMLYEITKGEAIVTTDVGQHQMWAAQ
YYPLKTPDKWVTSGGLGTMGFGFPAAIGAQIAKPDELVIAIVGDAGFQMTLQELSVLKEHSLPVKVFILNNEALGMVRQW
QDEFYNQRYSHSLLSCQPDFVALANAYGIKGVRIDDPLLAKKQLQHAIQLQEPVVIDCRVLQSEKVMPMVAPGKGVHQME
GVEKR

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=555, Percent_Identity=23.963963963964, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI21361361, Length=513, Percent_Identity=24.1715399610136, Blast_Score=140, Evalue=2e-33,
Organism=Escherichia coli, GI87081685, Length=566, Percent_Identity=45.9363957597173, Blast_Score=518, Evalue=1e-148,
Organism=Escherichia coli, GI1790104, Length=561, Percent_Identity=41.1764705882353, Blast_Score=432, Evalue=1e-122,
Organism=Escherichia coli, GI1786717, Length=541, Percent_Identity=34.9353049907579, Blast_Score=315, Evalue=6e-87,
Organism=Escherichia coli, GI1787096, Length=544, Percent_Identity=27.5735294117647, Blast_Score=195, Evalue=7e-51,
Organism=Escherichia coli, GI1788716, Length=484, Percent_Identity=24.3801652892562, Blast_Score=139, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI17531299, Length=485, Percent_Identity=29.0721649484536, Blast_Score=168, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI17531301, Length=485, Percent_Identity=29.0721649484536, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17542570, Length=558, Percent_Identity=24.1935483870968, Blast_Score=137, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6323755, Length=573, Percent_Identity=44.6771378708551, Blast_Score=479, Evalue=1e-136,
Organism=Saccharomyces cerevisiae, GI6320816, Length=495, Percent_Identity=25.4545454545455, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6323163, Length=490, Percent_Identity=24.4897959183673, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19922626, Length=570, Percent_Identity=25.0877192982456, Blast_Score=153, Evalue=4e-37,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 61869; Mature: 61738

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSKTEEKLATGAQLLLEALEKEGVEVIFGYPGGAVLPLYDALYDCEIPHILTRHEQGAI
CCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCCCHHHHCCCCCCE
HAAEGYARITGNPGVVIATSGPGATNVITGLADAMIDSLPLVVFTGQVATTLIGSDAFQE
EECCCEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEECHHHHHHHCCCHHCC
ADIMGLTMPVTKHNYQVRKASDLPRIIKEAFHIARTGRPGPVVIDLPKDMVVEQGERCSD
CCEEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHCCCCCCC
VQMDLPGYNPNYEPNLLQINKLLQAIQVANKPLILAGAGVLHAKASKELTSFARKYEIPV
CEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEEEHHHHHHHHHHHHHCCCHH
VHTLLGLGGFPPDDELFLGMGGMHGSYTANMALYECDLLINIGARFDDRLTGNLAYFAKE
HHHHHHCCCCCCCCCEEEECCCCCCCEECCEEEEEEEEEEECCCCCCCCCCCCEEEEEEC
ATVAHIDIDPAEIGKNVPTEIPIVASAKQALQVLLQPEGSKENHHEWISLLKNRKEKYPF
CEEEEEECCHHHHCCCCCCCCCEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCC
SYKRNSESIKPQYAIDMLYEITKGEAIVTTDVGQHQMWAAQYYPLKTPDKWVTSGGLGTM
CCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEECCCCCCCHHCCCCCCCCC
GFGFPAAIGAQIAKPDELVIAIVGDAGFQMTLQELSVLKEHSLPVKVFILNNEALGMVRQ
CCCCHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHH
WQDEFYNQRYSHSLLSCQPDFVALANAYGIKGVRIDDPLLAKKQLQHAIQLQEPVVIDCR
HHHHHHHHHHHCHHHCCCCCHHHHHHHCCCCCEEECCHHHHHHHHHHHHHCCCCEEEEEE
VLQSEKVMPMVAPGKGVHQMEGVEKR
EECCCCCCEEECCCCCCHHCCCCCCC
>Mature Secondary Structure 
SSKTEEKLATGAQLLLEALEKEGVEVIFGYPGGAVLPLYDALYDCEIPHILTRHEQGAI
CCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCCCHHHHCCCCCCE
HAAEGYARITGNPGVVIATSGPGATNVITGLADAMIDSLPLVVFTGQVATTLIGSDAFQE
EECCCEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEECHHHHHHHCCCHHCC
ADIMGLTMPVTKHNYQVRKASDLPRIIKEAFHIARTGRPGPVVIDLPKDMVVEQGERCSD
CCEEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHCCCCCCC
VQMDLPGYNPNYEPNLLQINKLLQAIQVANKPLILAGAGVLHAKASKELTSFARKYEIPV
CEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEEEHHHHHHHHHHHHHCCCHH
VHTLLGLGGFPPDDELFLGMGGMHGSYTANMALYECDLLINIGARFDDRLTGNLAYFAKE
HHHHHHCCCCCCCCCEEEECCCCCCCEECCEEEEEEEEEEECCCCCCCCCCCCEEEEEEC
ATVAHIDIDPAEIGKNVPTEIPIVASAKQALQVLLQPEGSKENHHEWISLLKNRKEKYPF
CEEEEEECCHHHHCCCCCCCCCEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCC
SYKRNSESIKPQYAIDMLYEITKGEAIVTTDVGQHQMWAAQYYPLKTPDKWVTSGGLGTM
CCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEECCCCCCCHHCCCCCCCCC
GFGFPAAIGAQIAKPDELVIAIVGDAGFQMTLQELSVLKEHSLPVKVFILNNEALGMVRQ
CCCCHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHH
WQDEFYNQRYSHSLLSCQPDFVALANAYGIKGVRIDDPLLAKKQLQHAIQLQEPVVIDCR
HHHHHHHHHHHCHHHCCCCCHHHHHHHCCCCCEEECCHHHHHHHHHHHHHCCCCEEEEEE
VLQSEKVMPMVAPGKGVHQMEGVEKR
EECCCCCCEEECCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377; 1577690; 9298659 [H]