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The map label for this gene is leuA [H]

Identifier: 52143944

GI number: 52143944

Start: 1378270

End: 1379790

Strand: Direct

Name: leuA [H]

Synonym: BCZK1285

Alternate gene names: 52143944

Gene position: 1378270-1379790 (Clockwise)

Preceding gene: 52143945

Following gene: 52143943

Centisome position: 26.0

GC content: 38.26

Gene sequence:

>1521_bases
ATGAAGAAAATTTTGTTCATGGATACGACGCTACGTGATGGCGAACAATCACCAGGAGTAAATTTAAATGAACAAGAGAA
ATTGCAAATTGCAAGGCAATTAGAGAGACTAGGGATTCATGTCATGGAAGCTGGCTTTGCAGCAGCTTCCGAAGGGGATT
TTCAATCGGTAAAGCGCATCGCAAATACAATTCAAAATGCTACAGTTATGAGTTTAGCAAGAGCGAAAGAAAGTGATATT
CGAAGAGCATATGAAGCTGTGAAAGGTGCGGTATCTCCGCGTTTACACGTATTTTTAGCTACGAGTGATATTCATATGAA
GTATAAACTTTGTATGTCAAAAGAAGATGTATTAGATAGTATTTATCGATCGGTTACACTTGGGAAATCGTTATTTCCGA
CAGTACAATTTTCAGCAGAAGATGCAACAAGAACAGCAAGAGATTTTTTAGCTGAAGCGGTAGAAGTTGCGATTCGTGCA
GGAGCGAATGTAATTAATATTCCAGATACAGTTGGGTATACGAATCCAGAAGAATATTATTCTCTTTTTAAATATTTACA
AGAATCCGTTCCTTCATATGAAAAAGCAATTTTCTCTTGTCATTGTCATGATGATCTTGGGATGGCGGTAGCAAATTCAT
TAGCTGCAGTTGAAGGCGGGGCGTTGCAAGTAGAAGGAACAATTAATGGAATTGGAGAAAGAGCAGGGAATGCGGCGTTA
GAAGAGGTTGCAGTTGCTCTTCATATTAGAAAAGATTTCTATAAAGCAGAGCCTTCTATGACGCTAAAAGAAATTAAAGC
GACAAGTACATTAGTAAGCAGATTAACAGGTATGGTTGTACCGAAAAATAAAGCGATTGTTGGAGCAAATGCATTCGCTC
ATGAATCAGGCATTCATCAAGATGGTGTATTAAAAGAAGTGACAACATATGAAATTATTGAACCAGCGCTAGTAGGCGAA
TCTCAAAATCTATTTGTACTTGGAAAACATTCTGGGCGTCACGCGTTTACAGAAAAAATGAAAGAACTTGGCTATGAATT
TACAAACGACGAGCGGGATGCAGTATTTGAAGCATTTAAAAAATTAGCTGATCGTAAGAAGGAAATTACGGAGGAAGATT
TACGAGCACTTATGCTTGGTGAAGCAGCATTTGCGGCTCAGCAATATAACATTACGCAATTGCAAGTACATTTCGTATCA
AATAGTACACAATGTGCGACAGTTGTACTAAAAGATGAGGAAGGAAATGTATTCGAAGATGCAGCTACTGGCTCTGGTAG
TATTGAAGCGATTTATAATGCAATCCAAAGAATTTTAGGATTGGAATGTGAATTGGCGGATTATCGCATACAATCTATTA
CACAAGGTCAAGATGCACTGGCTCATGTTCATGTTGAATTAAAAGAAGGAGCACATCAAGTATCAGGTTTTGGTGTTGCA
CAAGACGTATTGGAAGCGTCGGCAAGAGCATATGTCCACGCAGCAGGGAAATTGAAATCTTTTATCCAGCTTGTGAAATA
A

Upstream 100 bases:

>100_bases
AATGAGAAAGAAAATGAACATGAAATCGAAGTAGTTGGACGTAAATTACGTGAAATGATGCCTTTCGTACAGCCGCGAGT
AAAGGCAGGGGTCAAATAAT

Downstream 100 bases:

>100_bases
GTGTTTGATAGGTGAGAGGAGCTATTCTCACCTATCAAATTAAAGTTCTGAAAATTCAGTTAAAAGGAGTGTTGAAATTG
GAAAAACGTATTGTTTGTTT

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 506; Mature: 506

Protein sequence:

>506_residues
MKKILFMDTTLRDGEQSPGVNLNEQEKLQIARQLERLGIHVMEAGFAAASEGDFQSVKRIANTIQNATVMSLARAKESDI
RRAYEAVKGAVSPRLHVFLATSDIHMKYKLCMSKEDVLDSIYRSVTLGKSLFPTVQFSAEDATRTARDFLAEAVEVAIRA
GANVINIPDTVGYTNPEEYYSLFKYLQESVPSYEKAIFSCHCHDDLGMAVANSLAAVEGGALQVEGTINGIGERAGNAAL
EEVAVALHIRKDFYKAEPSMTLKEIKATSTLVSRLTGMVVPKNKAIVGANAFAHESGIHQDGVLKEVTTYEIIEPALVGE
SQNLFVLGKHSGRHAFTEKMKELGYEFTNDERDAVFEAFKKLADRKKEITEEDLRALMLGEAAFAAQQYNITQLQVHFVS
NSTQCATVVLKDEEGNVFEDAATGSGSIEAIYNAIQRILGLECELADYRIQSITQGQDALAHVHVELKEGAHQVSGFGVA
QDVLEASARAYVHAAGKLKSFIQLVK

Sequences:

>Translated_506_residues
MKKILFMDTTLRDGEQSPGVNLNEQEKLQIARQLERLGIHVMEAGFAAASEGDFQSVKRIANTIQNATVMSLARAKESDI
RRAYEAVKGAVSPRLHVFLATSDIHMKYKLCMSKEDVLDSIYRSVTLGKSLFPTVQFSAEDATRTARDFLAEAVEVAIRA
GANVINIPDTVGYTNPEEYYSLFKYLQESVPSYEKAIFSCHCHDDLGMAVANSLAAVEGGALQVEGTINGIGERAGNAAL
EEVAVALHIRKDFYKAEPSMTLKEIKATSTLVSRLTGMVVPKNKAIVGANAFAHESGIHQDGVLKEVTTYEIIEPALVGE
SQNLFVLGKHSGRHAFTEKMKELGYEFTNDERDAVFEAFKKLADRKKEITEEDLRALMLGEAAFAAQQYNITQLQVHFVS
NSTQCATVVLKDEEGNVFEDAATGSGSIEAIYNAIQRILGLECELADYRIQSITQGQDALAHVHVELKEGAHQVSGFGVA
QDVLEASARAYVHAAGKLKSFIQLVK
>Mature_506_residues
MKKILFMDTTLRDGEQSPGVNLNEQEKLQIARQLERLGIHVMEAGFAAASEGDFQSVKRIANTIQNATVMSLARAKESDI
RRAYEAVKGAVSPRLHVFLATSDIHMKYKLCMSKEDVLDSIYRSVTLGKSLFPTVQFSAEDATRTARDFLAEAVEVAIRA
GANVINIPDTVGYTNPEEYYSLFKYLQESVPSYEKAIFSCHCHDDLGMAVANSLAAVEGGALQVEGTINGIGERAGNAAL
EEVAVALHIRKDFYKAEPSMTLKEIKATSTLVSRLTGMVVPKNKAIVGANAFAHESGIHQDGVLKEVTTYEIIEPALVGE
SQNLFVLGKHSGRHAFTEKMKELGYEFTNDERDAVFEAFKKLADRKKEITEEDLRALMLGEAAFAAQQYNITQLQVHFVS
NSTQCATVVLKDEEGNVFEDAATGSGSIEAIYNAIQRILGLECELADYRIQSITQGQDALAHVHVELKEGAHQVSGFGVA
QDVLEASARAYVHAAGKLKSFIQLVK

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=493, Percent_Identity=45.2332657200811, Blast_Score=404, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6320071, Length=365, Percent_Identity=29.3150684931507, Blast_Score=148, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6320019, Length=361, Percent_Identity=29.3628808864266, Blast_Score=146, Evalue=6e-36,
Organism=Saccharomyces cerevisiae, GI6324225, Length=528, Percent_Identity=24.4318181818182, Blast_Score=137, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6324682, Length=529, Percent_Identity=24.1965973534972, Blast_Score=131, Evalue=2e-31,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 55401; Mature: 55401

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKILFMDTTLRDGEQSPGVNLNEQEKLQIARQLERLGIHVMEAGFAAASEGDFQSVKRI
CCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCHHHHHHH
ANTIQNATVMSLARAKESDIRRAYEAVKGAVSPRLHVFLATSDIHMKYKLCMSKEDVLDS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEHHHHCCHHHHHHH
IYRSVTLGKSLFPTVQFSAEDATRTARDFLAEAVEVAIRAGANVINIPDTVGYTNPEEYY
HHHHHHCCCHHCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHH
SLFKYLQESVPSYEKAIFSCHCHDDLGMAVANSLAAVEGGALQVEGTINGIGERAGNAAL
HHHHHHHHHCCHHHHHHEEEECCCHHHHHHHHHHHHCCCCCEEEEECHHHHHHHCCHHHH
EEVAVALHIRKDFYKAEPSMTLKEIKATSTLVSRLTGMVVPKNKAIVGANAFAHESGIHQ
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCEEECCCCEEEECCHHHHCCCCCC
DGVLKEVTTYEIIEPALVGESQNLFVLGKHSGRHAFTEKMKELGYEFTNDERDAVFEAFK
CCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHH
KLADRKKEITEEDLRALMLGEAAFAAQQYNITQLQVHFVSNSTQCATVVLKDEEGNVFED
HHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEEEEEEECCCCCEECC
AATGSGSIEAIYNAIQRILGLECELADYRIQSITQGQDALAHVHVELKEGAHQVSGFGVA
CCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHEEEEEEHHHCHHHHCCCHHH
QDVLEASARAYVHAAGKLKSFIQLVK
HHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MKKILFMDTTLRDGEQSPGVNLNEQEKLQIARQLERLGIHVMEAGFAAASEGDFQSVKRI
CCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCHHHHHHH
ANTIQNATVMSLARAKESDIRRAYEAVKGAVSPRLHVFLATSDIHMKYKLCMSKEDVLDS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEHHHHCCHHHHHHH
IYRSVTLGKSLFPTVQFSAEDATRTARDFLAEAVEVAIRAGANVINIPDTVGYTNPEEYY
HHHHHHCCCHHCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHH
SLFKYLQESVPSYEKAIFSCHCHDDLGMAVANSLAAVEGGALQVEGTINGIGERAGNAAL
HHHHHHHHHCCHHHHHHEEEECCCHHHHHHHHHHHHCCCCCEEEEECHHHHHHHCCHHHH
EEVAVALHIRKDFYKAEPSMTLKEIKATSTLVSRLTGMVVPKNKAIVGANAFAHESGIHQ
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCEEECCCCEEEECCHHHHCCCCCC
DGVLKEVTTYEIIEPALVGESQNLFVLGKHSGRHAFTEKMKELGYEFTNDERDAVFEAFK
CCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHH
KLADRKKEITEEDLRALMLGEAAFAAQQYNITQLQVHFVSNSTQCATVVLKDEEGNVFED
HHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEEEEEEECCCCCEECC
AATGSGSIEAIYNAIQRILGLECELADYRIQSITQGQDALAHVHVELKEGAHQVSGFGVA
CCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHEEEEEEHHHCHHHHCCCHHH
QDVLEASARAYVHAAGKLKSFIQLVK
HHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA