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The map label for this gene is hisZ [H]

Identifier: 52143940

GI number: 52143940

Start: 1383196

End: 1384458

Strand: Direct

Name: hisZ [H]

Synonym: BCZK1289

Alternate gene names: 52143940

Gene position: 1383196-1384458 (Clockwise)

Preceding gene: 161763542

Following gene: 52143938

Centisome position: 26.09

GC content: 34.76

Gene sequence:

>1263_bases
ATGACAAAATGGAAACGGGCAAACCCAAATGGAACGAGAGATTATTTATTCGAAGAATGTACGTTAATTGAAGAAGTGGA
GCAAAAATTAAGGCGTACTTTTTTAGAGAGAGGTTATGAAGAAATAAGGACACCAACAATTGAATTTTATGATGTGTTCG
CCTTTCAAAGCAGGCCGATAGATGAAGAAAAGATGTATAAATTTTTTGATGAAAAGGGGCGGATTATCGTATTGCGTCCA
GATATGACAATTCCTTTAGCGAGAGTGATGGGAACGCAGAGGTGTGATACGCCACTTAAAGTGACGTATAGCGGAAATGT
ATTTCGAGCGAATGAGTCTCTTGCTGGAAAATATAATGAGATTGTACAAAGTGGTATTGAAGTTATCGGTATTGATAATG
TAAGAGCTGAAATTGAATGTGTTATAAGCGTCATTCAATCACTTCAAAAGTTGAAGGTGCAATCTTTTACAATAGAGATT
GGGCAAGTACAGTTATATAAATGTATTGTCAAAAAGCTCTCCATTCATGAGGAAGAAGAGAAAGTTCTTAGAACATATAT
TGAAAGTAAAAATTATGCCGCTTTATCCAATTTTATTAGAGATAAGAAATTAGATCGATGTGATGAAACAGTAAAGTTAC
TGGAGAAATTACCGAGGTTATTTGGGAATTTAGAAGTGATTGAGGAAGCAGAAAAACTCGCCTCAAGTAATGAAATGAAA
ATGGCTATTACAAGAGTGAAAGAAATATATGAAGCAATTGAAAAACTAGGCTATGGATCTTACATATCAATTGATTTAGG
AATGATTCAACATTTGGACTATTATACAGGTGTTATTTTCAAAGGGTATATATATGAAATTGGAGAAGAAATTGTTAGTG
GAGGAAGATATGATGAGTTAATTGGAAATTTTGGAGAAATGTTACCGGCTGTTGGACTCGCGGTGCAAGTTAATCAAATT
GTAAAGGCGCTGCAAGAGCAGCAAGAGCCATATGAACGAAAGAGAATAGATATTATGATTCATTACGAGTTAAATAGATT
AGCAGAAGCGGAACGATTACGAAATTTACTTCAAAAGGACGGGAAAAAAGTAGCGCTATCTTTATTCTCTAATTTAAATG
ATACTTTTCAATTTGCTAGAAAAAATCAAATCGTAACGGTCGTTGAGGCAAAGAGCGAATCATTAGTGGAATATGTGTGG
AAAGAGAAATGGGTCGTCCAGAAAGAAGGGGAAACTTCATGCGTAACATTCAAATTGCGTTAA

Upstream 100 bases:

>100_bases
AGAGCCTTGTAGGTATATTTTATCTACAGGGCTCTTTTTATTTTCGGTTTAGGTGCATGAAATAAGCATACATTAGAAAG
GGAAAGGTGTGAGAATTTAG

Downstream 100 bases:

>100_bases
CGAAAGGGAGATTAGAAAAACATGTGATTCCGCTCTTTGAGCAGATTGGAATTGATTGTTCAGAGCTCAAAAATAAAGGA
AGAAAACTTGTCTTTCAAAG

Product: ATP phosphoribosyltransferase regulatory subunit

Products: AMP; diphosphate; L-histidyl-tRNA(His)

Alternate protein names: NA

Number of amino acids: Translated: 420; Mature: 419

Protein sequence:

>420_residues
MTKWKRANPNGTRDYLFEECTLIEEVEQKLRRTFLERGYEEIRTPTIEFYDVFAFQSRPIDEEKMYKFFDEKGRIIVLRP
DMTIPLARVMGTQRCDTPLKVTYSGNVFRANESLAGKYNEIVQSGIEVIGIDNVRAEIECVISVIQSLQKLKVQSFTIEI
GQVQLYKCIVKKLSIHEEEEKVLRTYIESKNYAALSNFIRDKKLDRCDETVKLLEKLPRLFGNLEVIEEAEKLASSNEMK
MAITRVKEIYEAIEKLGYGSYISIDLGMIQHLDYYTGVIFKGYIYEIGEEIVSGGRYDELIGNFGEMLPAVGLAVQVNQI
VKALQEQQEPYERKRIDIMIHYELNRLAEAERLRNLLQKDGKKVALSLFSNLNDTFQFARKNQIVTVVEAKSESLVEYVW
KEKWVVQKEGETSCVTFKLR

Sequences:

>Translated_420_residues
MTKWKRANPNGTRDYLFEECTLIEEVEQKLRRTFLERGYEEIRTPTIEFYDVFAFQSRPIDEEKMYKFFDEKGRIIVLRP
DMTIPLARVMGTQRCDTPLKVTYSGNVFRANESLAGKYNEIVQSGIEVIGIDNVRAEIECVISVIQSLQKLKVQSFTIEI
GQVQLYKCIVKKLSIHEEEEKVLRTYIESKNYAALSNFIRDKKLDRCDETVKLLEKLPRLFGNLEVIEEAEKLASSNEMK
MAITRVKEIYEAIEKLGYGSYISIDLGMIQHLDYYTGVIFKGYIYEIGEEIVSGGRYDELIGNFGEMLPAVGLAVQVNQI
VKALQEQQEPYERKRIDIMIHYELNRLAEAERLRNLLQKDGKKVALSLFSNLNDTFQFARKNQIVTVVEAKSESLVEYVW
KEKWVVQKEGETSCVTFKLR
>Mature_419_residues
TKWKRANPNGTRDYLFEECTLIEEVEQKLRRTFLERGYEEIRTPTIEFYDVFAFQSRPIDEEKMYKFFDEKGRIIVLRPD
MTIPLARVMGTQRCDTPLKVTYSGNVFRANESLAGKYNEIVQSGIEVIGIDNVRAEIECVISVIQSLQKLKVQSFTIEIG
QVQLYKCIVKKLSIHEEEEKVLRTYIESKNYAALSNFIRDKKLDRCDETVKLLEKLPRLFGNLEVIEEAEKLASSNEMKM
AITRVKEIYEAIEKLGYGSYISIDLGMIQHLDYYTGVIFKGYIYEIGEEIVSGGRYDELIGNFGEMLPAVGLAVQVNQIV
KALQEQQEPYERKRIDIMIHYELNRLAEAERLRNLLQKDGKKVALSLFSNLNDTFQFARKNQIVTVVEAKSESLVEYVWK
EKWVVQKEGETSCVTFKLR

Specific function: Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine [H]

COG id: COG3705

COG function: function code E; ATP phosphoribosyltransferase involved in histidine biosynthesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. HisZ subfamily [H]

Homologues:

Organism=Homo sapiens, GI6996014, Length=360, Percent_Identity=24.7222222222222, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI15029520, Length=347, Percent_Identity=25.0720461095101, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI71993693, Length=441, Percent_Identity=22.9024943310658, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71993686, Length=440, Percent_Identity=22.9545454545455, Blast_Score=86, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6325290, Length=361, Percent_Identity=22.1606648199446, Blast_Score=91, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24643061, Length=451, Percent_Identity=25.0554323725055, Blast_Score=115, Evalue=8e-26,
Organism=Drosophila melanogaster, GI24643059, Length=469, Percent_Identity=24.3070362473348, Blast_Score=108, Evalue=7e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004517
- InterPro:   IPR004516 [H]

Pfam domain/function: PF00587 tRNA-synt_2b [H]

EC number: 6.1.1.21

Molecular weight: Translated: 48801; Mature: 48670

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKWKRANPNGTRDYLFEECTLIEEVEQKLRRTFLERGYEEIRTPTIEFYDVFAFQSRPI
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCHHHHHEEHHCCCCC
DEEKMYKFFDEKGRIIVLRPDMTIPLARVMGTQRCDTPLKVTYSGNVFRANESLAGKYNE
CHHHHHHHHCCCCCEEEECCCCCCHHHHHHCCCCCCCCEEEEECCCEEECCCHHHHHHHH
IVQSGIEVIGIDNVRAEIECVISVIQSLQKLKVQSFTIEIGQVQLYKCIVKKLSIHEEEE
HHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHEEEECHHHHHHHHHHHHHCCHHHH
KVLRTYIESKNYAALSNFIRDKKLDRCDETVKLLEKLPRLFGNLEVIEEAEKLASSNEMK
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHH
MAITRVKEIYEAIEKLGYGSYISIDLGMIQHLDYYTGVIFKGYIYEIGEEIVSGGRYDEL
HHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
IGNFGEMLPAVGLAVQVNQIVKALQEQQEPYERKRIDIMIHYELNRLAEAERLRNLLQKD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCEEEEEEECHHHHHHHHHHHHHHHHC
GKKVALSLFSNLNDTFQFARKNQIVTVVEAKSESLVEYVWKEKWVVQKEGETSCVTFKLR
CHHHHHHHHHCCCHHHHHHHCCCEEEEEECCHHHHHHHHHHHCCEEEECCCCEEEEEEEC
>Mature Secondary Structure 
TKWKRANPNGTRDYLFEECTLIEEVEQKLRRTFLERGYEEIRTPTIEFYDVFAFQSRPI
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCHHHHHEEHHCCCCC
DEEKMYKFFDEKGRIIVLRPDMTIPLARVMGTQRCDTPLKVTYSGNVFRANESLAGKYNE
CHHHHHHHHCCCCCEEEECCCCCCHHHHHHCCCCCCCCEEEEECCCEEECCCHHHHHHHH
IVQSGIEVIGIDNVRAEIECVISVIQSLQKLKVQSFTIEIGQVQLYKCIVKKLSIHEEEE
HHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHEEEECHHHHHHHHHHHHHCCHHHH
KVLRTYIESKNYAALSNFIRDKKLDRCDETVKLLEKLPRLFGNLEVIEEAEKLASSNEMK
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHH
MAITRVKEIYEAIEKLGYGSYISIDLGMIQHLDYYTGVIFKGYIYEIGEEIVSGGRYDEL
HHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
IGNFGEMLPAVGLAVQVNQIVKALQEQQEPYERKRIDIMIHYELNRLAEAERLRNLLQKD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCEEEEEEECHHHHHHHHHHHHHHHHC
GKKVALSLFSNLNDTFQFARKNQIVTVVEAKSESLVEYVWKEKWVVQKEGETSCVTFKLR
CHHHHHHHHHCCCHHHHHHHCCCEEEEEECCHHHHHHHHHHHCCEEEECCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): 8520 [C]

Specific activity: 121.8

Km value (mM): 0.06 {His}} 0.046 {His}} 0.03 {His}} 0.02 {His}} 0.017 {His}} 0.011 {His}} 0.008 {His}} 0.0014 {tRNAHis}} 0.00056 {tRNAHis}} 0.0016 {U73tRNAHisGUG}} 0.00031 {U73tRNAHisGUG}} 0.89 {ATP}} 0.74 {ATP}} 0.655 {ATP}} 0.5

Substrates: ATP; L-histidine; tRNA(His)

Specific reaction: ATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 1, 2, 4-Triazole-3-alanine; D-Histidine; Diphosphate; L-Histidinol; Mg2+; N-Acetyl histidine; NEM; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA